Organization engineering skin containing peripheral hematopoietic stem cells and method of preparing the same

A technology of peripheral blood stem cells and tissue-engineered skin, applied in medical science, prostheses, etc., can solve problems such as circulating fibroblasts and achieve the effects of improving healing quality, inhibiting excessive proliferation, and reducing scar hyperplasia

Inactive Publication Date: 2008-03-12
陕西艾尔肤组织工程有限公司
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Problems solved by technology

After searching, there are no reports on tissue engineered skin products and their applications involving circulating fibroblasts

Method used

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Embodiment Construction

[0021] The technical scheme of the present invention will be further described in detail in conjunction with experiments below.

[0022] Step 1. Obtain peripheral blood stem cells: Mix and dilute normal saline and heparin at a volume ratio of 2:1, take the patient's venous blood, mix it with the diluted heparin solution at a volume of 1:1, and use Percoll density gradient centrifugation. To isolate the peripheral blood stem cells in the blood, the specific operation is as follows:

[0023]Take 7ml of 1.073% (m / v) Percoll separation solution and add it to a centrifuge tube, absorb the diluted venous blood, and slowly add it dropwise on the surface of the Percoll liquid in an amount of 1:1 (V / V) to keep the interface between the two liquids clear , to avoid venous blood sedimentation at the bottom; after centrifugation at 2000 rpm for 20 minutes, the centrifuge tube is divided into four layers, including the uppermost layer of serum, the lower layer of mixed cells of monocytes a...

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Abstract

The present invention is a tissue engineering skin containing the peripheral blood stem cell and the preparation method. The present invention puts the peripheral blood stem cell and the skin fibroblast cell inside the biological frame. The active tissue engineering skin containing the peripheral blood stem cell is built by planting the epidermal cell on the surface. The tissue engineering skin is similar to the natural skin tissue in structure. Compared with the present skin replacing product, the present invention has properties of improving the blood supply in the wound and promoting the wound healing. The present invention can improve the elastic and toughness properties of the skin as well as the mechanical wear tolerance. The present invention can also reduce the scar proliferation and improve the healing quality. The present invention can improve the success rate of the artificial skin planting and can be effectively applied in repairing the skin defect, which is hard to heal.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a preparation method of tissue engineering skin containing peripheral blood stem cells. Background technique [0002] As a barrier between the human body and the outside world, the skin has many important functions. Skin defects caused by burns, trauma, chronic ulcers, etc. can cause infection at the slightest level, and can lead to serious consequences such as dehydration, electrolyte loss, decreased immune function, and even death. There are many insurmountable shortcomings in autologous skin transplantation used in traditional treatment, and the emergence of tissue engineered skin provides a new way for treatment. The selection of seed cells is one of the key issues in the current product research of tissue engineered skin. Due to the slow proliferation speed and long culture period of somatic cells in vitro, the curative effect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38A61L27/18
Inventor 金岩张勇杰陆伟刘鹏
Owner 陕西艾尔肤组织工程有限公司
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