6results about How to "Inhibit hyperproliferation" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to a rapid tissue propagation method for radix ranunculi ternati. The rapid propagation method for the radix ranunculi ternati tissue comprises the following steps: (1) seed selection and disinfection: selecting vigorous seeds, cleaning the seeds, soaking the seeds with mercury bichloride, disinfecting the seeds with alcohol, and finally washing the seeds with sterile water; (2) preparing a primary induction culture medium: preparing the primary induction culture medium; (3) seed induction and proliferation: inoculating the disinfected seeds on a primary induction culture medium, and culturing; after 4-6 weeks of culture, the seeds germinate into plantlets; transferring the plantlets to a subculture induction medium for multiplication culture, and subculturing once every 3-4 weeks; (4) rooting culture: transferring the proliferation seedlings to a rooting culture medium, and culturing for 2-3 weeks until the adventitious buds can root to form complete plants; and (5) seedling hardening and transplanting. The method can improve the rooting rate and shorten the propagation time at the same time.

The present application relates to an optimization method suitable for Agrobacterium transient infection conditions of Phalaenopsis, the explant of Phalaenopsis after Agrobacterium infection treatment is placed in the first co-culture condition for culture; the cut around, tissue edge and surface state of the explant are observed; when the cut around appears browning, and at least one of the accompanying tissue edge water spot or surface loss of luster, the host defense threshold is determined; after reaching the host defense threshold, the residual Agrobacterium liquid on the surface of the explant is removed, and the explant is transferred to the second co-culture condition for continuous culture. Realize the precise regulation of co-culture, improve the accuracy of threshold identification, and optimize the matching degree of Agrobacterium infection. Adopt hierarchical film removal, asymmetric co-culture and partition suction and other operations, inhibit the excessive proliferation of Agrobacterium, accurately remove the residual bacterial liquid, while ensuring the activity of the explant, significantly improve the infection efficiency, expression stability and method repeatability.

The application provides a fusion protein for inhibiting a Wnt signal path and application thereof, and relates to the field of biological medicines, and comprises the following: a first peptide segment, wherein the first peptide segment comprises a FU1 fragment; a second peptide segment, wherein the second peptide segment comprises a DRPB fragment; and the first peptide segment is connected with the second peptide segment. The prepared fusion protein can effectively inhibit the Wnt signal path, can inhibit excessive proliferation of cells under a pathological condition of abnormal activation of Wnt, and can effectively regulate the Wnt signal path.

The application discloses a 3-hydroxypyrrolidine derivative and a preparation method and application thereof. The structural formula of the 3-hydroxypyrrolidine derivative is shown in formula (I) or formula (II). The 3-hydroxypyrrolidine derivatives shown in formula (I) and formula (II) are non-diastereoisomers. The 3-hydroxypyrrolidine derivative provided by the application can inhibit the proliferation of RAW264.7 cells, and further inhibit the excessive proliferation of macrophages in immune cells to a certain extent. The 3-hydroxypyrrolidine diastereoisomers are obtained through one-pot reaction of a suitable reaction substrate under the action of a copper catalyst, the reaction condition is simple, and a good yield is obtained, so that the problems of needing to pass through multiple synthesis steps, high reaction risk, low reaction efficiency and low yield in the prior art are solved, and a new green synthesis route for the preparation of the 3-hydroxypyrrolidine derivative is provided.

The utility model discloses a sample constant-temperature preservation device for collecting pathogenic microorganisms, and particularly relates to the technical field of medical instruments, the sample constant-temperature preservation device comprises a moving assembly, the rear part of the moving assembly is fixedly connected with a fixed assembly, the front part of the moving assembly is rotatably connected with a placing assembly, and the moving assembly is fixedly connected with the fixed assembly. A containing plate is slidably connected to an inner cavity of the moving assembly, a constant-temperature assembly is fixedly connected to the inner cavity of the moving assembly, and a scraping assembly is rotationally connected to the front portion of the constant-temperature assembly. According to the sample constant-temperature storage device for collecting pathogenic microorganisms, the interior of the first shell can be cooled through the designed power assembly and the constant-temperature assembly, so that the interior of the first shell is always kept at a low temperature, and when the sample is placed for a long time, the sample cannot be deteriorated, so that the sample is prevented from losing the effect; the specimen for microbiological examination can be preserved at low temperature for 24 hours at most, and the temperature range is beneficial to keeping the activity of microorganisms.

This invention discloses a collagen-based anti-corrosion coating with tissue repair properties, its preparation method, and its applications, belonging to the field of medical coating technology. This invention designs a novel self-healing anti-corrosion coating and loads collagen onto the coating using excipients, achieving a deep integration of anti-corrosion protection and biological function. The prepared collagen-based anti-corrosion coating is firmly bonded to a magnesium alloy substrate via covalent bonds. After 4 weeks of in vivo implantation, no cracking or detachment was observed, the stent structure maintained good integrity, and it provides continuous radial support, offering sufficient time for vascular repair. This provides a feasible solution for the clinical translation of next-generation biodegradable magnesium-based vascular stents.