Preparation method and application of amino-rich stent material modified by copper 4-carboxyphenyl porphyrin, blood vessel stent material and application

A technology of copper carboxyphenylporphyrin and copper phenylporphyrin, which is applied in the field of surface modification of biomaterials, can solve problems such as increasing the risk of late thrombosis, and achieve the effect of inhibiting the proliferation of smooth muscle cells and promoting the adhesion and proliferation of endothelial cells

Active Publication Date: 2019-04-23
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The second-generation drug-eluting stents have solved the problem of in-stent restenosis very well, but the drug-eluting stents release drugs to inhibit intimal hyperplasia and at the same time inhibit the healing of the endothelial cell layer, thereby increasing the risk of late thrombosis. risk

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  • Preparation method and application of amino-rich stent material modified by copper 4-carboxyphenyl porphyrin, blood vessel stent material and application
  • Preparation method and application of amino-rich stent material modified by copper 4-carboxyphenyl porphyrin, blood vessel stent material and application
  • Preparation method and application of amino-rich stent material modified by copper 4-carboxyphenyl porphyrin, blood vessel stent material and application

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preparation example Construction

[0036] The invention provides a method for preparing an amino-rich scaffold material modified by copper tetracarboxyphenylporphyrin, comprising:

[0037] Dissolve tris-hydroxymethylaminomethane (Tris-base) in single distilled water (RO) to form a 1.2 mg / ml Tris solution. RO water is also called pure water, that is, water filtered through a reverse osmosis membrane. The pore size of the reverse osmosis membrane is generally between 10A and 100A, so it can remove more than 95% of ionic impurities. The water used in the embodiment of the present invention is RO water, which ensures the stable progress of each reaction.

[0038] The weighed dopamine (PDA) and hexamethylenediamine (HD) were dissolved in a Tris solution to prepare a PDA@HD solution. The @ in PDA@HD means PDA and HD. In some embodiments of the present invention, the dosage ratio of dopamine, hexamethylenediamine and Tris solution is 1 mg:2.5 mg:1 mL. The PDA@HD solution can ensure oxidative cross-linking on the su...

Embodiment 1

[0048] The invention provides a tetracarboxyphenylporphyrin copper-modified amino-rich scaffold material, which is mainly prepared through the following steps:

[0049] The dimethylformamide (DMF) of 15ml is blended with the single distilled water (RO) of 35ml to prepare an organic solvent for subsequent use;

[0050] Weigh 15mg copper tetracarboxyphenyl porphyrin (CuTCPP) and dissolve it in the organic solution made in the first step of 15ml, then adjust the pH value with ammonia water to make the pH 7 ± 0.5, and filter off the insoluble tetracarboxyphenyl porphyrin Porphyrin copper, makes tetracarboxyphenyl porphyrin copper solution;

[0051] Weigh 0.0257g of N-hydroxysuccinimide (NHS) and 0.0887g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and dissolve in 5ml of In single distilled water (RO), the activator was obtained;

[0052] Add 1.5ml of the activator prepared in the third step to 15ml of tetracarboxyphenylporphyrin copper solution, then put ...

Embodiment 2

[0055] The invention provides a tetracarboxyphenylporphyrin copper-modified amino-rich scaffold material, which is mainly prepared through the following steps:

[0056] The dimethylformamide (DMF) of 15ml is blended with the single distilled water (RO) of 30ml to prepare the organic solvent for subsequent use;

[0057] Weigh 15mg copper tetracarboxyphenyl porphyrin (CuTCPP) and dissolve it in the organic solution made in the first step of 10ml, then adjust the pH value with ammonia water to make the pH 7±0.5, filter off the insoluble tetracarboxyphenyl porphyrin Porphyrin copper, makes tetracarboxyphenyl porphyrin copper solution;

[0058] Weigh 0.0257g of N-hydroxysuccinimide (NHS) and 0.0887g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and dissolve in 5ml of In single distilled water (RO), the activator was obtained;

[0059] Add 3ml of the activator prepared in the third step to 15ml of tetracarboxyphenylporphyrin copper solution, then put the ami...

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Abstract

The invention relates to a preparation method and application of an amino-rich stent material modified by copper 4-carboxyphenyl porphyrin, a blood vessel stent material and application, and belongs to the technical field of biological material surface modification. The preparation method of the amino-rich stent material modified by copper 4-carboxyphenyl porphyrin includes the steps that copper 4-carboxyphenyl porphyrin is dissolved in an organic solvent, the pH value of a solution is adjusted to be 7+ / -0.5, so that a copper 4-carboxyphenyl porphyrin solution is obtained. After an activatingagent and copper 4-carboxyphenyl porphyrin are mixed, the amino-rich stent material is put into a mixed solution for a reaction, taken out, flushed and sterilized. The surface of the blood vessel stent material is modified with copper 4-carboxyphenyl porphyrin, in this way, good biological compatibility is realized, repairing of endothelial cells can be promoted, and excessive proliferation of smooth muscle cells can be inhibited.

Description

technical field [0001] The invention relates to the technical field of surface modification of biological materials, and in particular to a preparation method and application of an amino-rich stent material modified by copper tetracarboxyphenylporphyrin, and a vascular stent material and its application. Background technique [0002] Cardiovascular disease has become one of the main causes of abnormal death in human beings. Stent intervention is the main method to treat atherosclerosis, but there are still many problems after stent intervention. The first generation of bare metal stents mainly faced the problem of in-stent restenosis. The second-generation drug-eluting stents have solved the problem of in-stent restenosis very well, but the drug-eluting stents release drugs to inhibit intimal hyperplasia and at the same time inhibit the healing of the endothelial cell layer, thereby increasing the risk of late thrombosis. risk. [0003] Therefore, multifunctional stent ma...

Claims

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Application Information

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IPC IPC(8): A61L31/16
CPCA61L31/16A61L2300/216A61L2300/416A61L2400/18
Inventor 杨苹向攀陈江戴胜何双廖玉珍刘鲁英江婷黄楠
Owner SOUTHWEST JIAOTONG UNIV
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