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Method for planting esoderma/endothelial cell on inner surface of artificial blood vessel

A technology of artificial blood vessels and endothelial cells, which is applied to human tubular structure devices, tissue cell/virus culture devices, blood vessels, etc., to achieve the effect of inhibiting excessive proliferation and increasing adhesion

Inactive Publication Date: 2005-01-05
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These conditions are quite different from those of static cultured endothelial cells in vitro

Method used

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  • Method for planting esoderma/endothelial cell on inner surface of artificial blood vessel
  • Method for planting esoderma/endothelial cell on inner surface of artificial blood vessel

Examples

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Effect test

example 1

[0034] Example 1. Static inoculation on mouse carotid artery vascular graft material depleted of cell matrix, followed by stepwise increasing fluid shear

[0035] stress while the vessel rotates about its central axis to culture endothelial cells

[0036] The carotid artery of Wistar rats whose endothelial cells were removed by freezing method was inoculated at 10 dyns / cm 2 Rabbit primary arterial endothelial cells acclimated to shear stress in the intima were inoculated as follows: the intima of the mouse carotid artery from which the endothelial cells were removed by freezing was washed three times with PBS solution, and the cultured primary rabbit endothelial cells were washed with 0.1 % trypsinization, collect the cells, the cell concentration is about 1×10 6 cells / cm 2 , inoculated the collected cells on the intima of blood vessels at 37°C, 5% CO 2 Static culture in the incubator for about 3 days, after the cells have fused into a single layer, then, the inoculated end...

example 2

[0037] Example 2. Inoculation on the mouse carotid artery vascular graft material with the cell matrix removed and simultaneously applying a stepwise increase in fluid shear stress, while the blood vessel was rotated around its central axis to cultivate endothelial cells

[0038] Take the carotid artery of Wistar rats whose endothelial cells were removed by freezing method, and inoculate rabbit primary arterial endothelial cells on the intima. The cultured primary rabbit endothelial cells were digested with 0.1% trypsin, the cells were collected, and the cell concentration was about 1×10 6 cells / cm 2 , the collected cells were inoculated into the intima of the blood vessel, and at the same time, a stepwise increase of fluid shear stress was applied to the endothelial cells, and the initial value of the shear stress was about 5dyns / cm 2 , the step is to increase 0.1dyns / cm every 1h 2 , the required time is about 12h, and then change the step to increase 1.0dyns / cm every 1h 2...

example 3

[0039] Example 3. Inoculation and simultaneous application of pulsating, step-wise increasing fluid shear stress on goat carotid artery graft material without cell matrix, while the vessel rotates around its central axis to cultivate endothelial cells

[0040] Take the goat carotid artery from which endothelial cells have been removed by enzymatic digestion, and inoculate the primary cultured human umbilical vein endothelial cells on the intima. , digest the cultured primary human umbilical vein endothelial cells with 0.1% trypsin, collect the cells, the cell concentration is about 1×10 6 cells / cm 2 , the collected cells were inoculated into the intima of the blood vessel, and a pulsating, stepwise increasing fluid shear stress was applied to the endothelial cells at the same time, the pulsation frequency was 80 Hz, and the initial value of the loading shear stress was about 5 dyns / cm 2 , the step is to increase 0.3dyns / cm every 1h 2 , the time required is about 12h, and the...

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PUM

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Abstract

A method for implanting the endothelial cells on the inner surface of artificial blood vessel features that a biomechanical method is used, that is, an external pulsive fluid shearing force increased step by step acts on the planted endothelial cells to make them be attached well on the inner surface of artificial blood vessel in advance.

Description

technical field [0001] The invention relates to a method for planting endothelial / endothelial-like cells on the inner surface of artificial blood vessels. Background technique [0002] Cardiovascular system disease is one of the diseases with the highest incidence rate in the world, and it is also the leading cause of death at present. The main treatment method is vascular transplantation. Every year in the United States alone, more than 570,000 coronary artery bypass grafts are performed. In addition, the implementation of bypass surgery also requires a large number of peripheral vascular grafts. This has resulted in a significant demand for small caliber (<6 mm) vascular grafts. [0003] The purpose of vascular tissue engineering is to construct an ideal vascular graft in vitro, without immune rejection after transplantation, and to maintain the long-term patency of the grafted vessel lumen. Cardiovascular surgery requires vascular grafts of various diameters as repai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06A61K48/00C12M3/00C12N1/00
Inventor 潘君王远亮叶志义蔡绍皙覃素华吴科达
Owner CHONGQING UNIV
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