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Baicalin-modified amino-rich scaffold material as well as preparation method and application thereof

A scaffold material, the technology of baicalin, which is applied in the field of baicalin-modified amino-rich scaffold materials and its preparation, can solve the problems of difficult endothelial regeneration and intima tissue repair, stent stenosis, and increased risk of late thrombosis, achieving easy operation, The effect of wide application range and simple preparation process

Inactive Publication Date: 2018-06-01
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stent intervention is the main method for the treatment of atherosclerosis, but there are still many problems after stent intervention: the first generation of bare metal stents mainly faces the problem of in-stent restenosis
The second-generation drug-eluting stents have solved the problem of in-stent restenosis very well, but the drug-eluting stents release drugs to inhibit intimal hyperplasia and at the same time inhibit the healing of the endothelial cell layer, thereby increasing the risk of late thrombosis. risk
[0007] However, it is still difficult for such biomolecule-modified scaffolds to truly achieve the goal of radical endothelial regeneration and intimal tissue repair with normal functions in the face of complex diseased vascular microenvironment
The reason may be that in the functional design of stents, it is generally ignored that the working environment after stent injection is caused by the vascular microenvironment of oxidative stress.

Method used

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  • Baicalin-modified amino-rich scaffold material as well as preparation method and application thereof
  • Baicalin-modified amino-rich scaffold material as well as preparation method and application thereof
  • Baicalin-modified amino-rich scaffold material as well as preparation method and application thereof

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preparation example Construction

[0037] The embodiment of the present invention also provides a preparation method of the baicalin-modified amino-rich scaffold material. It comprises the following steps: mixing baicalin solution and activator, activating, mixing amino-rich scaffold material and activated baicalin solution, and grafting.

[0038] Preferably, the above-mentioned baicalin solution can be obtained through the following steps: mixing baicalin, water and ammonia water, and adjusting the pH value of the mixed solution system after mixing to 6.5-6.9. Preferably, the above-mentioned water is single distilled water (RO), that is, the water obtained after one distillation; the above-mentioned ammonia water is ammonia water diluted 400 times.

[0039] In the embodiment of the present invention, ammonia water is mixed with baicalin. The reason is that the molecules of baicalin are unstable and will decompose under alkaline conditions, while ammonia water is a weak base and has little effect on baicalin. It...

Embodiment 1

[0055] Dilute baicalin with distilled water and ammonia water 400 times until the pH of the system is 6.7 to obtain a baicalin solution with a concentration of 0.5 mg / mL.

[0056] Dissolve 0.0776g EDC and 0.0257g NHS in 5 mL of single distilled water as activator. The activator and baicalin solution were mixed in a volume ratio of 1:10, and activated at 25°C for 20 minutes.

[0057] Put amino-rich stainless steel with h of 1.5 mm and Φ of 10 mm into a Φ70 mm petri dish, then add 15 mL of baicalin solution activated for 20 min until it submerges the amino-rich stainless steel, and graft for 15 h under static conditions. The surface of the material was washed three times with RO water to wash off the unstable BCL on the ungrafted, and obtain the baicalin-modified amino-rich scaffold material.

Embodiment 2

[0059] Dilute baicalin with single distilled water and ammonia water diluted 400 times until the pH of the system is 6.5 to obtain a baicalin solution.

[0060] Dissolve 0.07 g EDC and 0.02 g NHS in 5 mL of single distilled water as activators. The activator and baicalin solution were mixed in a volume ratio of 1:8, and activated at 10°C for 25 minutes.

[0061] Put the amino-rich stainless steel whose h is 1 mm and Φ is 8 mm into a petri dish, then add 10 mL of baicalin solution activated for 25 minutes until it submerges the amino-rich stainless steel, and graft for 24 hours under static conditions. The surface of the material was washed three times with RO water to wash off the unstable BCL on the ungrafted, and obtain the baicalin-modified amino-rich scaffold material.

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Abstract

The invention relates to a baicalin-modified amino-rich scaffold material as well as a preparation method and an application thereof and belongs to the technical field of medical scaffolds. The preparation method of the baicalin-modified amino-rich scaffold material comprises steps as follows: a baicalin solution is mixed with an activating agent to be activated, an amino-rich scaffold material and the activated baicalin solution are mixed, and grafting is performed. The method adopts a simple process and is simple to operate and wide in application range. The baicalin-modified amino-rich scaffold material prepared with the method simultaneously has multiple functions of anti-oxidative stress, anti-inflammation, endothelial apoptosis inhibition, inhibition of excessive proliferation of smooth muscle cells and the like. The baicalin-modified amino-rich scaffold material is used for improving the microenvironment of blood vessels, can notably improve the biocompatibility of the scaffoldmaterial and can inhibit adverse reactions such as oxidative stress, inflammation, restenosis and the like after the scaffold is implanted into the body of a patient.

Description

technical field [0001] The invention relates to the field of medical scaffolds, and in particular to a baicalin-modified amino-rich scaffold material and a preparation method and application thereof. Background technique [0002] According to the statistics of the World Health Organization, cardiovascular disease has become one of the main causes of abnormal death of human beings, accounting for 30% of the number of deaths from various causes. Stent intervention is the main method for the treatment of atherosclerosis, but there are still many problems after stent intervention: the first generation of bare metal stents mainly faces the problem of in-stent restenosis. The second-generation drug-eluting stents have solved the problem of in-stent restenosis very well, but the drug-eluting stents release drugs to inhibit intimal hyperplasia and at the same time inhibit the healing of the endothelial cell layer, thereby increasing the risk of late thrombosis. risk. [0003] With...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/28A61L27/54
CPCA61L27/28A61L27/54A61L2300/232A61L2300/41A61L2300/416
Inventor 杨苹刘鲁英陈江廖玉珍黄楠
Owner SOUTHWEST JIAOTONG UNIV
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