Bifidobacterium animalis capable of relieving psoriasis and application of bifidobacterium animalis
A technology of animal bifidobacteria and microbial strains, applied in the field of microorganisms, can solve problems such as obvious adverse reactions
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Embodiment 1
[0060] Embodiment 1: the acquisition of animal bifidobacterium
[0061] Specific steps are as follows:
[0062] 1. Screening
[0063] Taking human feces from Wuxi, Jiangsu as samples, the samples were pretreated and stored in 20% glycerin in a -80°C refrigerator. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL to contain 0.05 Gradual dilution of 9 g / L normal saline with g / L cysteine, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies of Bifidobacterium animalis to the MRS solid Purify by streaking on the medium, pick a single colony and transfer it to MRS liquid medium (containing 0.05g / L cysteine) for enrichment, and preserve it in 30% glycerol to obtain strain CCFM1148; among them, the typical colony of Bifidobacterium animalis It is milky white, irregular, round and convex, and smooth.
[0064] 2. Identification
[0065] The genome of bacterial strain CCFM1148 was e...
Embodiment 2
[0066] Example 2: Effect of Bifidobacterium animalis on proliferation of psoriasis-like cortex-forming cells
[0067] Specific steps are as follows:
[0068] After rewarming the frozen human immortalized cortex-forming cells at 37°C, centrifuge at 1000rpm for 5min, and take the pellet; the pellet was washed with 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL chain After washing once with DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin, the cells were resuspended and counted to obtain cell weight. Suspension: Inoculate the cell resuspension into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcultured wh...
Embodiment 3
[0072] Example 3: Effect of Bifidobacterium animalis on NF-κB nuclear translocation in psoriasiform cortex-forming cells
[0073] Specific steps are as follows:
[0074] After rewarming the frozen human immortalized cortex-forming cells at 37°C, centrifuge at 1000rpm for 5min, and take the pellet; the pellet was washed with 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL chain After washing once with DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin, the cells were resuspended and counted to obtain cell weight. Suspension: Inoculate the cell resuspension into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were su...
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