Lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells
A technology of Lactobacillus and side cheese, applied in the field of microorganisms, can solve the problems of obvious adverse reactions
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Embodiment 1
[0059] Embodiment 1: the acquisition of Lactobacillus paracasei
[0060] Specific steps are as follows:
[0061] 1. Screening
[0062] Taking human feces from Wuxi, Jiangsu as samples, the samples were pretreated and stored in 20% glycerin in a -80°C refrigerator. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL to contain 0.05 Gradual dilution of 9 g / L normal saline with g / L cysteine, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, and pick typical colonies of Lactobacillus paracasei on the MRS solid Purify by streaking on the culture medium, pick a single colony and transfer it to MRS liquid medium (containing 0.05g / L cysteine) for enrichment, and preserve it in 30% glycerol to obtain bacterial strain CCFM1147; among them, the typical colony of Lactobacillus paracasei It is milky white, irregular, round and convex, and smooth.
[0063] 2. Identification
[0064] The genome of bacterial ...
Embodiment 2
[0065] Example 2: Effect of Lactobacillus paracasei on the proliferation of psoriasis-like cortex-forming cells
[0066] Specific steps are as follows:
[0067] After rewarming the frozen HaCaT cells at 37°C, centrifuge at 1000rpm for 5min to take the pellet; wash the pellet with DMEM containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL streptomycin After the medium was washed once, the cells were resuspended in DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin and counted to obtain a cell resuspension; Inoculate the resuspension of cells into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcultured ...
Embodiment 3
[0071] Example 3: Effect of Lactobacillus paracasei on psoriasis-like cortex-forming cells NF-κB nuclear translocation
[0072] Specific steps are as follows:
[0073] After rewarming the frozen HaCaT cells at 37°C, centrifuge at 1000rpm for 5min to take the pellet; wash the pellet with DMEM containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL streptomycin After the medium was washed once, the cells were resuspended in DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin and counted to obtain a cell resuspension; Inoculate the resuspension of cells into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcu...
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