Preparation method of tissue engineering skin containing appendant organs

Abstract

The invention relates to a preparation method of tissue engineering skin containing appendant organs. In the invention, the preparation method comprises the following steps of: inoculating amniotic mesenchyme stem cells, amniotic mesenchyme stem cells induced to the directions of hair papillae, amniotic epithelial cells and amniotic epithelial cells induced to the directions of the epithelia of the sweat gland into a gel solution, then inoculating the amniotic epithelial cells and the amniotic epithelial cells induced by keratinocyte in a warp direction on the surface of a structure of the hair follicle and the sweat gland after culturing by a dermal layer by induced culture forming the structure of the hair follicle and the sweat gland, and obtaining the tissue engineering skin with the structure of the hair follicle and the sweat gland after culturing. The skin has elasticity, toughness, vigorous cellular metabolism, strong multiplication capacity, sufficient secretion of extracellular matrices, tight linkage of the cuticular layer, the inophragma and the cells of the dermal layer and little possibility of falling off, enhances the success rate of transplantation, can promote the healing of a wound surface, enhances the effects of restoration and replacement and has the function of regulating the immunological rejection of a receptor; by the contained structure of the hair follicle and the sweat gland, the function of the skin is more complete; the adopted amniotic tissues are postpartum wastes and have extensive source, strong cell multiplication capability and multiplepassage frequency; and the invention is beneficial to the mass production of seed cells and lowers the industrialization cost.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of medical biological materials, and in particular relates to a method for constructing tissue-engineered skin with appendages by using cells derived from amniotic membrane. Background technique [0002] The skin is the largest organ of the human body. It has the functions of protecting the body, regulating body temperature, and excreting waste. It is an important tissue that covers and protects the body surface and internal organs. Defects and abnormalities of the skin and mucous membranes are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital malformations. Normal skin contains a well-differentiated epidermis and dermis. An ideal healing wound should have a complete epidermis, dermis, hair follicles, sweat glands and other appendage structures; the epidermis can act as a barrier and protect, preventing harmful substances from passing through the ...

AI Technical Summary

Problems solved by technology

[0005] Because epidermal cells tend to lose their keratinization characteristics during in vitro culture, the epidermal layer of the prepared tissue-engineered skin becomes more brittle and cannot function normally. This is also a technical problem that has plagued the field of tissue-engineered skin for many years.