The invention discloses a submicron bone
pathological sample three-dimensional
reconstruction method based on integration of
cutting, measurement and analysis, and the method comprises the following steps: step 1, carrying out an integral
dyeing scheme on hard bone tissues which are not decalcified so as to realize uniform marking of cells and matrix components in a
full thickness range of a sample; step 2, carrying out
dehydration and
resin embedding treatment on the
bone tissue sample subjected to integral
dyeing, so that the
bone tissue sample becomes a firm block suitable for ultrathin
cutting; 3, ultra-precise continuous
cutting and in-situ imaging are carried out in a cutting-measuring-imaging integrated in-situ
machining mode; 4, after the images of all slice
layers are acquired, performing digital
processing and three-dimensional reconstruction on a large amount of acquired two-dimensional image data; 5, after the high-resolution three-dimensional reconstruction model of the
bone tissue is obtained, quantitative analysis and visual display are conducted on the high-resolution three-dimensional reconstruction model of the bone tissue. According to the method, the resolution and continuity of bone tissue
pathological analysis are greatly improved, and the defects of traditional
pathological two-dimensional diagnosis are effectively overcome.