156results about How to "No effect on growth" patented technology

The invention discloses a concentric-ring sprayer structure for material vapor phase epitaxy, which solves the problem that the large-area deposition region provides a uniform flow field of a precursor gas mixture in a large-substrate or multi-substrate crystal growth process. The sprayer structure comprises more than one independent air inlet pipeline, wherein each air inlet pipeline is provided with a controller for monitoring and regulating inlet gas flow speed and flow rate; the bottom of the sprayer is provided with an air outlet baffle; more than one concentric ring is arranged in the sprayer; independent cavities are formed among the concentric rings and are mutually separated; the top end of each concentric ring is connected with one independent air inlet pipeline; and the air outlet baffle at the bottom end of each concentric ring is provided with one or more air outlets. The air sources are mutually separated and independently controlled; and the multi-sprayer integrated use mode obviously improves the quality of the large-area deposited grown crystal, and greatly enhances the production efficiency.

A composite herbicide for rape field contains the pyrimidine benzylamine kind of herbicide, at least one of quizalofop-ethyl, fluazifop-p-butyl, haloxyfop, fenoxaprop and sethoxydim, acetochlor, alachlor, napropamide, ehaprochlor, metolachlor, ethamet sulfuron and benazolin. Its advantages are high effect, broad spectrum, low dosage and high safety.

The invention relates to a feed, in particular to a wholewheat feed suitable for medium and big pigs of 50 kg before slaughter stage. The feed comprises the following components by mass percent of air dry matters: 0-2 percent of fish meal, 0-25 percent of bean pulp, 1-10 percent of rapeseed meal, 0-10 percent of bran coat, 0-30 percent of corn DDGS (distillers dried grains with soluble), 0-15 percent of whole milk rice bran, 0-60 percent of wheat, 0.3-1.5 percent of calcium carbonate, 0.5-1 percent of secondary calcium phosphate, 0-2 percent of soybean oil, 2 percent of premix and additive and 0/01-0.02 percent of wheat complex enzyme. The wholewheat feed has the benefits that firstly, corn is completely replaced by wheat through optimizing the feed formula, the growth of medium and big pigs is not influenced and the meat quality is remarkably improved; secondly, the feed active ingredients all conform to the feed hygienic standard and national standard, so that adverse effects can not be caused on the nutritional value of pork pigs; and thirdly, the pork pig raising cost is effectively lowered.

The invention relates to a heavy metal trapping agent in the field of environment protection. The heavy metal trapping agent is prepared from polymer containing a polyethylene diamine tetra-acetic acid unit, wherein by weight percentage, the content of the polyethylene diamine tetra-acetic acid unit is 45-100% of polymer; the polymer is homopolymer or copolymer; the polyethylene diamine tetra-acetic acid unit is a form of sodium salt, potassium salt or ammonium salt. The defect that a chelate compound of existing ethylenediamine tetra-acetic acid and the heavy metal is generally soluble is overcome, and the provided heavy metal trapping agent has strong heavy metal chelating power, and the solubility can be controlled by pH of the solution; moreover, the trapping agent is free of toxicity.

The invention discloses a dry farming direct seeding planting method of rice, which comprises the steps of preparing sprinkling irrigation equipment: the lifting micro-sprinkling irrigation equipment is buried under to-be-planted ground for 30-40cm; processing a field: straws of last stubbles of crops on the to be planted ground are cut into sections, the lengths of the straw sections are 2-20cm, the straw sections are broadcast in soil, a base fertilizer is applied on the soil, and the soil is subjected to ploughing and rotary tillage; preparing rice seeds and seeding: the selected rice seeds are sown on the processed soil uniformly, and then the soil is compacted; and performing sprinkling irrigation: the lifting micro-sprinkling irrigation equipment buried under the ground is used for irrigating the soil sown with the rice seeds, and the water content of the surface of the soil is 40-60%. Therefore, the precision irrigation to the crops is realized; excess water accumulation in the rice field is avoided; and the utilization ratio of water resources is increased. In addition, the straws are turned into organic fertilizers with the passage of time; straw turnover is realized indeed; the method contributes to improving the yield and quality of grains; and environmental pollution due to straw burning is avoided.

The invention discloses a method of constructing selection-marker-free self-luminescent mycobacterium abscessus and establishment of a corresponding in-vitro high-flux medicine-screening model. During the construction process, a key factor is the construction of a recombinant plasmid pOPAI1B, which contains a fused gene including a luminescent required enzyme gene LuxCDABE, a resistance screening gene Apr and an integrase gene Int; direct repeat sequences DifR and DifL at the two ends of the fused gene respectively; and a phage integration site attP and a replicon. The recombinant plasmid pOPAI1B is transformed into the mycobacterium abscessus and then is screened to obtain the selection-marker-free self-luminescent mycobacterium abscessus of which a resistance gene and the integrase gene are both lost. The self-luminescent mycobacterium abscessus can screen drugs in a high flux, can determine sterilizing or bacteria-resistant effects of the drug by means of detection of luminescent situation of bacteria, can be used for in-vitro drug activity detection, is free of any substrates, can continuously detect the same sample, is high in sensitivity and is strong in repeatability.

The invention discloses a telomeric protein polypeptide fragment with tumor cell killing activity and an application thereof. The polypeptide provided by the invention is (a) or (b) as follows: (a) polypeptide composed of amino acid sequence as shown in sequence 1 of a sequence table; and (b) polypeptide which is derived from sequence 1 relative to the tumor cell killing activity and replacing and/or missing and/or adding one or more amino acid residues for the amino acid sequence of the sequence 1. The experiment shows that the recombinant adenovirus which expresses the polypeptide provided by the invention can inhibit the growth of the tumor cell telomerase positive, induces the apoptosis of the tumor cell and inhibits the growth of tumors in vitro and vivo. The polypeptide provided by the invention has broad spectrum and specific telomerase positive tumor cell killing activity; and meanwhile, the polypeptide does not affect the growth and survival of normal human cell; therefore, the telomeric protein polypeptide fragment and the application thereof have significant meaning on the research of novel broad spectrum specific anticancer drugs.

The invention relates to a disinfectant for a pond water body, comprising the following components in parts by weight: 10-100 parts of potassium ferrate, 100-300 parts of polymeric aluminium, 200-500 parts of polymeric ferric sulphate and 100-700 parts of clinoptilolite. Ferrate used in the invention is taken as a non-chlorine water treatment agent, bacteria, viruses and algaes in water can be rapidly killed, organic matters, inorganic matters and heavy metal ions in the water also can be removed, and the effects of disinfection, decolourization, deodorization and coagulation assistant can be realized. The disinfectant for the pond water body disclosed by the invention has reasonable components and low cost, is simple to prepare and is convenient to use, development and application curative effects are assured, toxic and side effects are small, and application range is wide.

A composite herbicide for rape field is prepared from propionylnitrofen or isopropionylnitrofen and at least one of quizalofop-P-ethyl, fluazifop-P-butyl, haloxyfop, Biaoma and sethoxydim. Its advantages are broad spectrum, delaying resistance, low dosage and high safety.

The invention relates to soil sample collecting equipment, in particular to a soil sample collector for collecting a soil sample in a plough layer for various conventional soil sample analysis. The soil sample collector comprises a handle and a drilling body, wherein the handle comprises two hollow nested steel tubes; a first spring is accommodated in the outer-layer steel tube; an open pore is arranged on the upper wall of the outer-layer steel tube; one end of the inner-layer steel tube can be nested in the outer-layer steel tube in a reciprocating moving manner; the first spring is positioned between the end part of the inner-layer steel tube and the inner wall of the outer-layer steel tube; a positioning device positioned in the open pore when the drilling body is opened is arranged on a tube body of the inner-layer steel tube positioned in the outer-layer steel tube; the drilling body is formed by hinging two parts with the same structure; and the two parts are respectively connected with the inner-layer steel tube and the outer-layer steel tube. The soil sample collector has small size; the handle and the drilling body are compactly combined; when the soil sample collector is free from influencing the growth of peripheral plants when being used for collecting the soil samples on the surface layer of soil, and the advantages are especially remarkable when the crop plants are higher and the planting density is larger; and the soil sample collector can be also used for transplanting plants at the seedling stage.

The invention relates to a marking method of a selective breeding individual of turbot. The method comprises the following steps of: firstly, selecting a glue needle, a hangtag gun and a mark board, wherein the glue needle is 10mm and in a transverse H shape, the model number of the glue needle is MUXP-25, the model number of the hangtag gun is SAGA 70L, the mark board is made of Solisep white polyester composite paper, the sign board has a length of 2 centimeters and a width of 0.8 centimeter, and a mark code is a 5-bit identifier; secondly, selecting a turbot juvenile fish the body length of which is longer than 5 centimeters, and keeping the fish to have a swimming posture during marking; positioning an inserting point at a part having more muscles behind the left eye on the head of the fish body; reserving a length of 2-8 millimeters of the glue needle in vitro; and finally, putting the marked fish in a 10ppm florfenicol medicated bath for 2 hours, carrying out the medicated bath once each day and continuously carrying out the medicated bath for three days. The method has simple operation, low cost, high automation degree and smaller damage to the fish body. The retention rate of a mark is higher than 90 percent, and the effective recognition rate is higher than 90 percent. The normal activity of the turbot cannot be influenced, and the method can meet the requirements of the large-scale breeding and marking of the turbot.

The invention designs compound separated and extracted by marine fusarium (Fusarium sp. QY010) and application thereof. The compound is the active compound which is obtained by fermenting, separating and purifying the fusarium QY010, wherein the chemical name of the active compound is 5-butyl pyridine formic acid, and the active compound is obtained by screening the pseudomonas aeruginosa screening model QSIS2 and the chromobacterium type bacterial strain CV026. Within the concentration range that the pathogenic bacteria can not be killed, the compound has the double-inhibitory action to the quorum-sensing system of the pseudomonas aeruginosa and the chromobacterium, can restrain the pathogenic behavior of the bacterial quorum sensing regulation, can obviously reduce the yield of the chromobacterium and the mycetin, can reduce the virulence factors of the quorum-sensing regulation of the pseudomonas aeruginosa, and can weaken the drug resistance of the pathogenic bacteria. The invention has the advantages of being easy to obtain the preparation raw materials, easy to ferment and cultivate the bacterial strain, high in yield, and simple and easy to control the technical process for preparing the compound. Furthermore, the analogues derived by the compound can be used for the research and the development of novel antibacterial agents.

A mutagenic method with low-energy heavy ion radiation for culturing the seeds of Humi herb used to build the grazing land in shoal and nunja includes such steps as low-energy heavy ion radiation to the embryonic calli of Humi herb to lead and mutation of genetic material, differentiating to generate regenerated seedlings, growing in artificial greenhouse, screening excellent line by testing its characteristics including protein content, and comparing DNA variation to obtain a new variety.

The invention discloses a strain of bacillus amyloliquefaciens with an inhibiting effect on fusarium oxysporum and application of the bacillus amyloliquefaciens. The fusarium oxysporum (FON) is pathogenic bacteria of watermelon fusarium wilt, and biological control is a crucial method for controlling the watermelon fusarium wilt. A bacterial strain for inhibiting the FON is screened from wheat rhizosphere, and the bacterial strain is Bacillus amyloliquefaciens LZN01 through authentication. Experimental results show that the antibacterial rate of the Bacillus amyloliquefaciens LZN01 to the FONis 57.07%, and the spore germination inhibition ratio of the Bacillus amyloliquefaciens LZN01 to the FON is 86.21%. Under the conditions that the temperature is 20-60 DEG C and the pH value is 4-9, the antibacterial effect of a fermented supernatant fluid of the Bacillus amyloliquefaciens LZN01 is stable, and antibacterial substances of the supernatant fluid contain Myriocin, Sphingofungin E, Sphingofungin F, Sphingofungin C and Gabapentin. Therefore, the Bacillus amyloliquefaciens LZN01 has a better inhibiting ability to the FON, the antibacterial effect is stable, and the development and application value is larger in biological control of the watermelon fusarium wilt.

The invention relates to an interplanting method for selenium-enriched moringa oleifera and oil tea, and belongs to the technical field of crop cultivation. The interplanting method for selenium-enriched moringa oleifera and oil tea comprises the steps of (1) oil preparation and fertilizer applying, (2) interplanting, (3) fertilizer water management and (4) prevention of diseases and insects. Theinterplanting method adopts a '1+2' planting mode of moringa oleifera and oil tea, the land utilization rate is effectively increased, and stable and continuous high yield of the oil tea is promoted;the diseases and insects of the oil tea are greatly reduced, the growing environment of the oil tea is purified, the using of chemical pesticide is reduced, and the ecological environment is protected; the appearance of the oil tea planted by adopting the method is good, the oil tea is safe and pollution-free, the survival rate of the planted moringa oleifera is higher, and the moringa oleifera isselenium-enriched.

The invention belongs to the technical field of bioengineering, and particularly relates to a metabolic engineering strain for producing vitamin B6 as well as a construction method and application ofthe metabolic engineering strain. Two ways for synthesizing vitamin B6 from the beginning, which do not exist in the same organism in the nature, are organically integrated into escherichia coli, besides, enzyme PdxH playing a bridge role is knocked out, two parallel biosynthesis channels are formed, and the channel I is an endogenous way and is used for producing a product form of the vitamin B6;and the channel II is an exogenous way for producing the active form of the vitamin B6 for the vital movement of cells. In the constructed engineering strain, the expression of an exogenous pathway is moderately improved through a strong promoter and/or an optimized RBS sequence, so that the engineering strain has the same growth rate as an original strain. The capacity of the engineering strainfor producing the vitamin B6 through fermentation is greatly improved, and the engineering strain has high application and popularization value.

The invention discloses a dispersable oil suspending agent containing acetamiprid and carbosulfan and an application. Pesticide is mainly composed of active ingredients, additives and a dispensing medium. The active ingredients are composed of chloridized nicotine novel broad-spectrum pesticide acetamiprid and carbamic acid ester pesticide carbosulfan, wherein the weight ratio of the chloridized nicotine novel broad-spectrum pesticide acetamiprid to the carbamic acid ester pesticide carbosulfan is 1:3. Pests of fruits and vegetables such as citruses and multiple commercial crops such as corn, cotton, rice and sugarcane can be effectively prevented and treated through the combination, the insecticidal spectrum is wide, and the prevention and treatment effect on aphids is particularly excellent. The acetamiprid and the carbosulfan are compounded to form the dispersable oil suspending agent with 48% of effective ingredients, the prevention and control effect on cotton aphids is good, the fast-acting property is good, the lasting period is long, and no influence is caused to cotton growth.