156results about How to "No effect on growth" patented technology

The invention discloses a concentric-ring sprayer structure for material vapor phase epitaxy, which solves the problem that the large-area deposition region provides a uniform flow field of a precursor gas mixture in a large-substrate or multi-substrate crystal growth process. The sprayer structure comprises more than one independent air inlet pipeline, wherein each air inlet pipeline is provided with a controller for monitoring and regulating inlet gas flow speed and flow rate; the bottom of the sprayer is provided with an air outlet baffle; more than one concentric ring is arranged in the sprayer; independent cavities are formed among the concentric rings and are mutually separated; the top end of each concentric ring is connected with one independent air inlet pipeline; and the air outlet baffle at the bottom end of each concentric ring is provided with one or more air outlets. The air sources are mutually separated and independently controlled; and the multi-sprayer integrated use mode obviously improves the quality of the large-area deposited grown crystal, and greatly enhances the production efficiency.

A composite herbicide for rape field contains the pyrimidine benzylamine kind of herbicide, at least one of quizalofop-ethyl, fluazifop-p-butyl, haloxyfop, fenoxaprop and sethoxydim, acetochlor, alachlor, napropamide, ehaprochlor, metolachlor, ethamet sulfuron and benazolin. Its advantages are high effect, broad spectrum, low dosage and high safety.

The invention relates to a feed, in particular to a wholewheat feed suitable for medium and big pigs of 50 kg before slaughter stage. The feed comprises the following components by mass percent of air dry matters: 0-2 percent of fish meal, 0-25 percent of bean pulp, 1-10 percent of rapeseed meal, 0-10 percent of bran coat, 0-30 percent of corn DDGS (distillers dried grains with soluble), 0-15 percent of whole milk rice bran, 0-60 percent of wheat, 0.3-1.5 percent of calcium carbonate, 0.5-1 percent of secondary calcium phosphate, 0-2 percent of soybean oil, 2 percent of premix and additive and 0 / 01-0.02 percent of wheat complex enzyme. The wholewheat feed has the benefits that firstly, corn is completely replaced by wheat through optimizing the feed formula, the growth of medium and big pigs is not influenced and the meat quality is remarkably improved; secondly, the feed active ingredients all conform to the feed hygienic standard and national standard, so that adverse effects can not be caused on the nutritional value of pork pigs; and thirdly, the pork pig raising cost is effectively lowered.

The invention relates to a heavy metal trapping agent in the field of environment protection. The heavy metal trapping agent is prepared from polymer containing a polyethylene diamine tetra-acetic acid unit, wherein by weight percentage, the content of the polyethylene diamine tetra-acetic acid unit is 45-100% of polymer; the polymer is homopolymer or copolymer; the polyethylene diamine tetra-acetic acid unit is a form of sodium salt, potassium salt or ammonium salt. The defect that a chelate compound of existing ethylenediamine tetra-acetic acid and the heavy metal is generally soluble is overcome, and the provided heavy metal trapping agent has strong heavy metal chelating power, and the solubility can be controlled by pH of the solution; moreover, the trapping agent is free of toxicity.

The invention relates to a bottom mud repair and treatment material for an urban black and odorous river channel and a preparing method of the bottom mud repair and treatment material. The bottom mud repair and treatment material for the urban black and odorous river channel and the preparing method of the bottom mud repair and treatment material can be applied to the technical field of bottom mud repair of urban black and odorous river channels. Raw materials for preparing the filler include CaO2, MgO2, attapulgite clay, dry EM powder and nanometer titanium dioxide. All the raw materials are pretreated with specific steps respectively and then evenly mixed according to a specific proportion, and the powder material is formed. The repair material is evenly mixed with the bottom mud of the urban black and odorous river channel in situ, nitrogen, phosphate and organic matter in the bottom mud can be effectively removed, stinks can be eliminated, and the effect of controlling source pollution in the river channel can be achieved.

The invention relates to a convenient and rapid DNA extracting method of peanut health tissues and diseased tissues. The method comprises two steps of: sample materials preparation: testing the leaf and stem tissues, shell tissues or peanut cotyledons, the weight of which is 3-10 mg, adding the sample materials into a 1.5ml centrifugal tube preset with an alkaline lysate or enzymolysis liquid, and grinding by a grinding rod till the visible particles do not exist; DNA extracting liquid preparation: treating the ground sample materials by an alkaline decoction method or a enzymolysis method to obtain the DNA extracting liquid. The method is also suitable for peanut health tissues and diseased tissues. Compared with the DNA extracting method, the invention has short required time, the required time of single sample by the alkaline decoction method does not exceed 20min, and the required time of single sample by the enzymolysis method does not exceed 0.5h; the dosage of the sample is little, and each sample only needs 3-10mg; the method has no need of liquid nitrogen to grind, therefore the cost is low, and the requirements of peanut molecule marker auxiliary breeding and transgene breeding are satisfied. The invention has no influence on the plant growth or seed germination, and has significant meaning on the practicality of peanut molecule breeding technology.

The invention discloses a dry farming direct seeding planting method of rice, which comprises the steps of preparing sprinkling irrigation equipment: the lifting micro-sprinkling irrigation equipment is buried under to-be-planted ground for 30-40cm; processing a field: straws of last stubbles of crops on the to be planted ground are cut into sections, the lengths of the straw sections are 2-20cm, the straw sections are broadcast in soil, a base fertilizer is applied on the soil, and the soil is subjected to ploughing and rotary tillage; preparing rice seeds and seeding: the selected rice seeds are sown on the processed soil uniformly, and then the soil is compacted; and performing sprinkling irrigation: the lifting micro-sprinkling irrigation equipment buried under the ground is used for irrigating the soil sown with the rice seeds, and the water content of the surface of the soil is 40-60%. Therefore, the precision irrigation to the crops is realized; excess water accumulation in the rice field is avoided; and the utilization ratio of water resources is increased. In addition, the straws are turned into organic fertilizers with the passage of time; straw turnover is realized indeed; the method contributes to improving the yield and quality of grains; and environmental pollution due to straw burning is avoided.

The invention relates to an arrangement method of an ecological gabion chamber revetment type, and belongs to a revetment structure type in river training works. A stepped sandstone cushion layer is laid on the revetment of a river; a sinuous ecological gabion chamber is arranged on the stepped sandstone cushion layer; and plants are planted in the sinuous ecological gabion chamber. The arrangement method has the advantages of novel structure, reliable operation, wide application range, capabilities of improving the engineering safety and favorably improving water eco-environments of rivers and lakes and great application prospect.

The invention relates to a building method of genetic engineering acetic acid bacteria of recombinantly expressing coenzyme PQQ (pyrroloquinoline quinone) synthetic proteins. The PQQ biosynthetic proteins are PqqA, PqqB, PqqC, PqqD, PqqE, and functional equivalents thereof. An alcohol dehydrogenase promoter from acetobacter pasteurianus and a coenzyme PQQ synthetic protein gene cluster pqqABCDE sequence are orderly connected into plasmids which can be stably copied in the acetic acid bacteria, so as to recombine pBBR-padh-pqq; recombinant plasmid pBBR-padh-pqq is transferred into the acetic acid bacteria, so as to obtain the genetic engineering acetic acid bacteria of overexpressing the coenzyme PQQ synthetic proteins. Thus, the concentration of in vivo coenzyme PQQ of the acetic acid bacteria in the fermentation process of acetic acid is improved. Acetic acid fermentation is carried out by using the genetic engineering bacteria disclosed by the invention, the fermentation delay phase can be shortened, and the fermentation rate of the acetic acid is increased. Thus, the production cost is reduced.

The invention discloses an individual labeling method for selective breeding of tilapia. Firstly, an appropriate plastic pin, a tagging gun, a closed loop plastic tag and so on, are selected; secondly, Tricaine methane sulphonate with a concentration of 0.33g / L is used to anesthetize the tilapia; and then a fish body is labeled. The tilapia is kept a posture of swimming during the labeling and faces toward the left side; an insertion point is positioned at the front end on the back of the fish body; the closed loop plastic tag is sleeved on a gun needle; the plastic pin directly passes through the fish body to fix a label, and the length of the plastic pin exposed outside the fish body is between 2 and 4cm; and finally the labeled tilapia is placed into a 10 percent povidone iodine solution for soaking disinfection. The method has the advantages of simple operation, low cost, and high automation, has less damage degree to the fish body, ensures that the retention rate of the label is more than 90 percent and the effective recognition rate is more than 90 percent, can not affect the normal activity of the tilapia, and can meet the need of large-scale labeling of selective breeding of the tilapia.

Provided is a trimming method for elongated and upright trunks of pear trees. The method comprises following steps: creating a hedge frame mesh, filed planting of nursery stocks, cutting, bud picking, vertically binding lignified parts of new shoots to the mesh, bud-notching, basal angles and waist angles opening, thinning and branching, promoting formation of flower buds, and management of continued branchs of central branches and lateral branches. The trimming method for elongated and upright trunks of pear trees helps to realize high-density of plantation pear trees.

The invention discloses a method of constructing selection-marker-free self-luminescent mycobacterium abscessus and establishment of a corresponding in-vitro high-flux medicine-screening model. During the construction process, a key factor is the construction of a recombinant plasmid pOPAI1B, which contains a fused gene including a luminescent required enzyme gene LuxCDABE, a resistance screening gene Apr and an integrase gene Int; direct repeat sequences DifR and DifL at the two ends of the fused gene respectively; and a phage integration site attP and a replicon. The recombinant plasmid pOPAI1B is transformed into the mycobacterium abscessus and then is screened to obtain the selection-marker-free self-luminescent mycobacterium abscessus of which a resistance gene and the integrase gene are both lost. The self-luminescent mycobacterium abscessus can screen drugs in a high flux, can determine sterilizing or bacteria-resistant effects of the drug by means of detection of luminescent situation of bacteria, can be used for in-vitro drug activity detection, is free of any substrates, can continuously detect the same sample, is high in sensitivity and is strong in repeatability.

The invention discloses a mixed microbial agent for soil remediation, and a preparation method and application thereof. The mixed microbial agent comprises photosynthetic bacteria, lactobacillus bulgaricus, bacillus subtilis and beer saccharomycete biomass by certain ratio. The method comprises the following steps: uniformly mixing the lactobacillus bulgaricus, the bacillus subtilis and the beer saccharomycete biomass to server as a main agent, and then uniformly mixing the photosynthetic bacteria with an appropriate amount water to serve as an auxiliary agent. The mixed microbial agent has the characteristics of simple constituents, high easiness in preparation, low cost and good stability, and can effectively remove heavy metal cadmium ion in polluted soil. The preparation method is simple, convenient to operate, green and environmentally friendly, without three wastes pollution. The mixed microbial agent can reduce the activity of heavy metal cadmium in the soil, reduce or interdict the probability that the heavy metal cadmium enters crops, and reduce the harm to human body by the heavy metal cadmium. Compared with a blank control group, the content of the heavy metal cadmium in a mulberry leaf production base treated by the mixed microbial agent is reduced by 20.3 percent.

The invention discloses a telomeric protein polypeptide fragment with tumor cell killing activity and an application thereof. The polypeptide provided by the invention is (a) or (b) as follows: (a) polypeptide composed of amino acid sequence as shown in sequence 1 of a sequence table; and (b) polypeptide which is derived from sequence 1 relative to the tumor cell killing activity and replacing and / or missing and / or adding one or more amino acid residues for the amino acid sequence of the sequence 1. The experiment shows that the recombinant adenovirus which expresses the polypeptide provided by the invention can inhibit the growth of the tumor cell telomerase positive, induces the apoptosis of the tumor cell and inhibits the growth of tumors in vitro and vivo. The polypeptide provided by the invention has broad spectrum and specific telomerase positive tumor cell killing activity; and meanwhile, the polypeptide does not affect the growth and survival of normal human cell; therefore, the telomeric protein polypeptide fragment and the application thereof have significant meaning on the research of novel broad spectrum specific anticancer drugs.

The invention relates to a disinfectant for a pond water body, comprising the following components in parts by weight: 10-100 parts of potassium ferrate, 100-300 parts of polymeric aluminium, 200-500 parts of polymeric ferric sulphate and 100-700 parts of clinoptilolite. Ferrate used in the invention is taken as a non-chlorine water treatment agent, bacteria, viruses and algaes in water can be rapidly killed, organic matters, inorganic matters and heavy metal ions in the water also can be removed, and the effects of disinfection, decolourization, deodorization and coagulation assistant can be realized. The disinfectant for the pond water body disclosed by the invention has reasonable components and low cost, is simple to prepare and is convenient to use, development and application curative effects are assured, toxic and side effects are small, and application range is wide.

The invention relates to the technical field of water-based colour paste and provides water-based colour paste which is environmentally friendly, non-toxic, good in dispersing performance, strong in tinting strength and resistant to light, does not become thick, has anti-ultraviolet ray property, good weatherability and resistance to chemical attack and does not get mildew. The water-based colour paste is prepared from the following raw materials in parts by weight: 50-60 parts of pigment, 4-6 parts of wetting agent, 9-12 parts of dispersing agent, 6-10 parts of antioxidant, 0.3-0.45 part of antiseptic, 0.3-0.5 part of mildew preventive, 0.5-0.7 part of water-based defoaming agent, 6-8 parts of thickening and anti-settling agnet, 6-10 parts of ultraviolet light absorber and 30-60 parts of deionized water.

A composite herbicide for rape field is prepared from propionylnitrofen or isopropionylnitrofen and at least one of quizalofop-P-ethyl, fluazifop-P-butyl, haloxyfop, Biaoma and sethoxydim. Its advantages are broad spectrum, delaying resistance, low dosage and high safety.

The invention relates to soil sample collecting equipment, in particular to a soil sample collector for collecting a soil sample in a plough layer for various conventional soil sample analysis. The soil sample collector comprises a handle and a drilling body, wherein the handle comprises two hollow nested steel tubes; a first spring is accommodated in the outer-layer steel tube; an open pore is arranged on the upper wall of the outer-layer steel tube; one end of the inner-layer steel tube can be nested in the outer-layer steel tube in a reciprocating moving manner; the first spring is positioned between the end part of the inner-layer steel tube and the inner wall of the outer-layer steel tube; a positioning device positioned in the open pore when the drilling body is opened is arranged on a tube body of the inner-layer steel tube positioned in the outer-layer steel tube; the drilling body is formed by hinging two parts with the same structure; and the two parts are respectively connected with the inner-layer steel tube and the outer-layer steel tube. The soil sample collector has small size; the handle and the drilling body are compactly combined; when the soil sample collector is free from influencing the growth of peripheral plants when being used for collecting the soil samples on the surface layer of soil, and the advantages are especially remarkable when the crop plants are higher and the planting density is larger; and the soil sample collector can be also used for transplanting plants at the seedling stage.

The invention discloses a biological preparation method of a fermentation medium and rhamnolipid. The biological preparation method comprises the following steps: (1) inoculating a rhamnolipid producing strain seed solution into an iron limited fermentation medium, carrying out fermentation culturing, and adding nanoparticles into the fermentation medium; and (2) extracting the rhamnolipid of obtained fermentation liquor by using an acid precipitation freeze-drying method. A mutant strain which is weak in pathogenic ability and does not have quorum sensing genes lasR is used, synthesis of the rhamnolipid is stimulated by the suitable nanoparticles under the suitable culture conditions, secretion of the rhamnolipid can be promoted, and moreover, health risks in a production process is greatly reduced. Meanwhile, the biological preparation method has the characteristics of low cost and simplicity in operation.

The invention relates to a marking method of a selective breeding individual of turbot. The method comprises the following steps of: firstly, selecting a glue needle, a hangtag gun and a mark board, wherein the glue needle is 10mm and in a transverse H shape, the model number of the glue needle is MUXP-25, the model number of the hangtag gun is SAGA 70L, the mark board is made of Solisep white polyester composite paper, the sign board has a length of 2 centimeters and a width of 0.8 centimeter, and a mark code is a 5-bit identifier; secondly, selecting a turbot juvenile fish the body length of which is longer than 5 centimeters, and keeping the fish to have a swimming posture during marking; positioning an inserting point at a part having more muscles behind the left eye on the head of the fish body; reserving a length of 2-8 millimeters of the glue needle in vitro; and finally, putting the marked fish in a 10ppm florfenicol medicated bath for 2 hours, carrying out the medicated bath once each day and continuously carrying out the medicated bath for three days. The method has simple operation, low cost, high automation degree and smaller damage to the fish body. The retention rate of a mark is higher than 90 percent, and the effective recognition rate is higher than 90 percent. The normal activity of the turbot cannot be influenced, and the method can meet the requirements of the large-scale breeding and marking of the turbot.

The invention designs compound separated and extracted by marine fusarium (Fusarium sp. QY010) and application thereof. The compound is the active compound which is obtained by fermenting, separating and purifying the fusarium QY010, wherein the chemical name of the active compound is 5-butyl pyridine formic acid, and the active compound is obtained by screening the pseudomonas aeruginosa screening model QSIS2 and the chromobacterium type bacterial strain CV026. Within the concentration range that the pathogenic bacteria can not be killed, the compound has the double-inhibitory action to the quorum-sensing system of the pseudomonas aeruginosa and the chromobacterium, can restrain the pathogenic behavior of the bacterial quorum sensing regulation, can obviously reduce the yield of the chromobacterium and the mycetin, can reduce the virulence factors of the quorum-sensing regulation of the pseudomonas aeruginosa, and can weaken the drug resistance of the pathogenic bacteria. The invention has the advantages of being easy to obtain the preparation raw materials, easy to ferment and cultivate the bacterial strain, high in yield, and simple and easy to control the technical process for preparing the compound. Furthermore, the analogues derived by the compound can be used for the research and the development of novel antibacterial agents.

A mutagenic method with low-energy heavy ion radiation for culturing the seeds of Humi herb used to build the grazing land in shoal and nunja includes such steps as low-energy heavy ion radiation to the embryonic calli of Humi herb to lead and mutation of genetic material, differentiating to generate regenerated seedlings, growing in artificial greenhouse, screening excellent line by testing its characteristics including protein content, and comparing DNA variation to obtain a new variety.

The invention discloses a strain of bacillus amyloliquefaciens with an inhibiting effect on fusarium oxysporum and application of the bacillus amyloliquefaciens. The fusarium oxysporum (FON) is pathogenic bacteria of watermelon fusarium wilt, and biological control is a crucial method for controlling the watermelon fusarium wilt. A bacterial strain for inhibiting the FON is screened from wheat rhizosphere, and the bacterial strain is Bacillus amyloliquefaciens LZN01 through authentication. Experimental results show that the antibacterial rate of the Bacillus amyloliquefaciens LZN01 to the FONis 57.07%, and the spore germination inhibition ratio of the Bacillus amyloliquefaciens LZN01 to the FON is 86.21%. Under the conditions that the temperature is 20-60 DEG C and the pH value is 4-9, the antibacterial effect of a fermented supernatant fluid of the Bacillus amyloliquefaciens LZN01 is stable, and antibacterial substances of the supernatant fluid contain Myriocin, Sphingofungin E, Sphingofungin F, Sphingofungin C and Gabapentin. Therefore, the Bacillus amyloliquefaciens LZN01 has a better inhibiting ability to the FON, the antibacterial effect is stable, and the development and application value is larger in biological control of the watermelon fusarium wilt.

The invention discloses a method for combining large-diameter tree / tree house with multiple small trees and provides a method for combining small tree seedlings with a ground diameter of phi (1cm-3cm) into one tree and growing into a tree with a huge diameter in 5-10 years. Moreover, another function of the tree is that the tree is a new variety not existing in the nature. The method for combining large-diameter tree / tree house with multiple small trees comprises the technical procedure as follows: small tree seedlings with a ground diameter of phi (around 1cm-3cm) and a height of 1.5-3m are selected; then a pit is dug and a plurality of small tree seedlings are together placed in the pit; then two planes of two rind parts of 60mm sections of the trunks of two small tree seedlings in the most center which can be contacted are selected and flatly jointed; then 2-4 small holes are drilled in two contact points; crabsticks with a ground diameter of phi (1mm) are inserted into the holes; then the residual crabsticks are removed so that the crabsticks are aligned to an outer wall; then the crabsticks are fixed by thin ropes; 502 or white latex glue is coated between the crabsticks and the hole walls so that the crabsticks and the hole walls are firmly stuck; after fixation, the rope is unfastened; and so forth, a neighboring tree is placed closely and connected by crabsticks; afterthe connection is completed, the trees are bound by a soft rope; then the soft rope is removed in a half year so that the trees can grow naturally; after the trees grow higher, the height positions are connected by adopting the same method. The trunks are outwards bent at a required height and are fixed by bending plates with a bending angle of 120-150 degrees (as shown in diagram 3); then the bending plates are removed in a half year; and the trees are conventionally and carefully cultured; the crown is pruned; and the trees grow into a tree with a huge diameter in a plurality of years.

The invention relates to the technical field of aquatic organism selective breeding marking, and relates to a marking contrast method of an offshore intermediate breeding and development stage during a shellfish pedigree breeding process. The invention specifically relates to a shellfish pedigree breeding marking contrast method. According to the method, an environment-friendly plastic pad is adopted as a material for a geometric-shaped marker; different geometric holes are formed in the marker by cutting; and marking can be realized with the holes and the color of the marker. According to the method, the cost is low, and the marking is simple and clear. The marker can be accurately identified under a situation of foreign body adhesion. The recognition degree is high, and the marker meets production needs. The marker is convenient to manufacture, and is easy to carry. The marker can be widely and repeatedly applied. The marker has long preservation time and high preservation rate. Marking is carried out by tying the marker on a cultivation cage with a plastic tie, such that physiological damage to the shellfish is prevented, and the marker is stable and is prevented from falling and loss. Therefore, the stability of the marker is high. A marking rate and readable rate of the marker are both 100%, such that shellfish pedigree breeding large-scale industrialization development requirements are met. The method has an important significance in shellfish industry breeding.

The invention discloses an application of benzyl benzoate in preparation of bacterial colony induction activity inhibitors; in the method, an oceanobacillus sp. XC22919 fermented product is used for separation and extraction, and in a concentration range of 0-60 [mu]g / mL, bacterial growth of chromobacterium violaceum CV026 is not inhibited, but generation of chromobacterium violaceum purpurin canbe significantly decreased; and with gradual increase of the concentration, the effect of inhibiting generation of the purpurin is stronger; the effect of the compound in inhibiting colony induction is in a concentration dependence. In a concentration range of 0-100 [mu]g / mL, bacterial growth of pseudomonas aeruginosa PAO1 is not affected, but the expression of virulence factors of pseudomonas aeruginosa can be significantly reduced, the expression of pyocyanin, elastase, proteinase, biological body membranes and other virulence factors can be significantly reduced, and the effect of the compound in inhibiting colony induction is in the concentration dependence.

The invention relates to an interplanting method for selenium-enriched moringa oleifera and oil tea, and belongs to the technical field of crop cultivation. The interplanting method for selenium-enriched moringa oleifera and oil tea comprises the steps of (1) oil preparation and fertilizer applying, (2) interplanting, (3) fertilizer water management and (4) prevention of diseases and insects. Theinterplanting method adopts a '1+2' planting mode of moringa oleifera and oil tea, the land utilization rate is effectively increased, and stable and continuous high yield of the oil tea is promoted;the diseases and insects of the oil tea are greatly reduced, the growing environment of the oil tea is purified, the using of chemical pesticide is reduced, and the ecological environment is protected; the appearance of the oil tea planted by adopting the method is good, the oil tea is safe and pollution-free, the survival rate of the planted moringa oleifera is higher, and the moringa oleifera isselenium-enriched.

The invention discloses a DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii. The research object of the method is small in size, and does not need to be put to death; the economic character of the object can be bred at the earlier stage to enhance the breeding efficiency; the consumption of tissue materials for DNA extraction is extremely low; and the extracted DNA is good in quality, thereby meeting the requirements of biological research. The technical key points of the invention are as follows: the DNA in-vivo sampling method for young Hyriopsis cumingii comprises the following steps: 1) peeling mantle tissues of young Hyriopsis cumingii; 2) extracting the genome DNA of the peeled tissues; 3) detecting the extracted DNA; and 4) culturing the young Hyriopsis cumingii.

The invention belongs to the technical field of bioengineering, and particularly relates to a metabolic engineering strain for producing vitamin B6 as well as a construction method and application ofthe metabolic engineering strain. Two ways for synthesizing vitamin B6 from the beginning, which do not exist in the same organism in the nature, are organically integrated into escherichia coli, besides, enzyme PdxH playing a bridge role is knocked out, two parallel biosynthesis channels are formed, and the channel I is an endogenous way and is used for producing a product form of the vitamin B6;and the channel II is an exogenous way for producing the active form of the vitamin B6 for the vital movement of cells. In the constructed engineering strain, the expression of an exogenous pathway is moderately improved through a strong promoter and / or an optimized RBS sequence, so that the engineering strain has the same growth rate as an original strain. The capacity of the engineering strainfor producing the vitamin B6 through fermentation is greatly improved, and the engineering strain has high application and popularization value.

The invention discloses a dispersable oil suspending agent containing acetamiprid and carbosulfan and an application. Pesticide is mainly composed of active ingredients, additives and a dispensing medium. The active ingredients are composed of chloridized nicotine novel broad-spectrum pesticide acetamiprid and carbamic acid ester pesticide carbosulfan, wherein the weight ratio of the chloridized nicotine novel broad-spectrum pesticide acetamiprid to the carbamic acid ester pesticide carbosulfan is 1:3. Pests of fruits and vegetables such as citruses and multiple commercial crops such as corn, cotton, rice and sugarcane can be effectively prevented and treated through the combination, the insecticidal spectrum is wide, and the prevention and treatment effect on aphids is particularly excellent. The acetamiprid and the carbosulfan are compounded to form the dispersable oil suspending agent with 48% of effective ingredients, the prevention and control effect on cotton aphids is good, the fast-acting property is good, the lasting period is long, and no influence is caused to cotton growth.