Bacillus amyloliquefaciens with inhibiting effect on fusarium oxysporum and application of bacillus amyloliquefaciens

Abstract

The invention discloses a strain of bacillus amyloliquefaciens with an inhibiting effect on fusarium oxysporum and application of the bacillus amyloliquefaciens. The fusarium oxysporum (FON) is pathogenic bacteria of watermelon fusarium wilt, and biological control is a crucial method for controlling the watermelon fusarium wilt. A bacterial strain for inhibiting the FON is screened from wheat rhizosphere, and the bacterial strain is Bacillus amyloliquefaciens LZN01 through authentication. Experimental results show that the antibacterial rate of the Bacillus amyloliquefaciens LZN01 to the FONis 57.07%, and the spore germination inhibition ratio of the Bacillus amyloliquefaciens LZN01 to the FON is 86.21%. Under the conditions that the temperature is 20-60 DEG C and the pH value is 4-9, the antibacterial effect of a fermented supernatant fluid of the Bacillus amyloliquefaciens LZN01 is stable, and antibacterial substances of the supernatant fluid contain Myriocin, Sphingofungin E, Sphingofungin F, Sphingofungin C and Gabapentin. Therefore, the Bacillus amyloliquefaciens LZN01 has a better inhibiting ability to the FON, the antibacterial effect is stable, and the development and application value is larger in biological control of the watermelon fusarium wilt.

Description

Technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens and its application, in particular to a Bacillus amyloliquefaciens with the effect of inhibiting watermelon-specific Fusarium oxysporum (FON) and its application. The invention belongs to the field of agricultural biotechnology. Background technique [0002] Watermelon occupies an important position in the world's horticultural production. China is the largest country in watermelon production and consumption, with an annual planting area of ​​2 million ha 2 , The annual output is about 70 million tons (Huang Chunyan et al., 2016). Watermelon fusarium wilt is one of the important factors restricting watermelon production. The pathogenic bacteria is Fusarium oxysporum f.sp.niveum (FON) (Yu Tianxiang, 2004). With the expansion of watermelon planting area year by year, continuous cropping has led to an increase in the number of pathogens and aggravated the occurrence of watermelon wilt. E...

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Problems solved by technology

At present, the disease-resistant varieties cultivated in China mainly include Xinong 8, Jingkang 1, and Xiuli (Ding Jiancheng et al., 2005). However, due to the extremely low genetic diversity of watermelon, the disease-resistant germplasm resources that can be selected for breeding Very limited, and the conventional breeding cycle is long, the progress is slow, and the coefficient of technical difficulty is high. The disease-resistant varieties bred are only relatively disease-resistant, have low vitality, and are easily affected by the surrounding environment (Wang Wancheng, 2008), which cannot fundamentally solve the problem. Problem: Chemical agents can play a significant role in the prevention and treatment of Fusarium wilt of watermelon (Yan Leiyan et al., 2016), but the drug residues they produce cause environmental pollution, harm non-target organisms, and even destroy the ecological balance, seriously restricting the green development of the watermelon industry. Development; grafting cultivation using the roots of plants such as gourds and wax gourds has a good effect on the control of watermelon wilt and has been widely used (Sakata et al., 2007), but the workload is large, which affects the taste and quality of the fruit; Physical control of Fusarium wilt of watermelon can achieve a certain control effect, but the workload is large and time-consuming (Sun Yichun et al., 2005)

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