46results about How to "Low inhibition rate" patented technology

A magnetic field device and method for inhibiting angiogenesis and retarding growth rates of cancerous tumors present in mammals. The apparatus includes a frame, and a wire consisting of electrically conducting material. The wire is wrapped around the frame to form a coil. A source of AC current is connected to a transformer to vary the AC voltage. The AC current is passed through a bridge rectifier and then to the coil of wire enabling a DC rectified wave magnetic field to be produced therefrom. The method employs the use of an apparatus which is capable of producing a magnetic field of a particular nature which has been proven in animal studies to affect angiogenesis and retard the growth rate of cancerous tumors.

Enzyme immunoassay method for detecting Kebaiwei includes (1) synthesizing half antigen and artificial antigen; (2) preparing polyclonal antibody, monoclonal antibody, or gene engineering antibody of possessing specificity to Kebaiwei; (3) preparing enzyme labeled antigen; (4) using second antibody to coat enzyme labeled plate and close the plate to save Kebaiwei; reaction is carried out between anti Kebaiwei antibody and second antibody and fixed on the enzyme labeled plate; (5) washing out free object, adding sample to be tested, and enzyme labeled antigen or Kebaiwei labeled sample, and enzyme labeled antigen; (6) washing out free object, adding substrate of enzyme and developer. Intensity of enzyme promoting color reaction is proportional to quantity of enzyme labeled antigen bound on antibody, and is inversely proportional to content of Kebaiwei in sample. Features are: long term storage, increasing sensitivity, accuracy, simple and quick test operation.

The invention discloses a nanometer antibody resistant to pseudomonas aeruginosa ectotoxin A and application of the nanometer antibody. The nanometer antibody has 3 unique complementary determining regions CDR1, CDR2 and CDR3, and the application relates to application of the nanometer antibody in preparation of a drug resistant to the pseudomonas aeruginosa ectotoxin A. The nanometer antibody resistant to the pseudomonas aeruginosa ectotoxin A has specific identification and combination capacity to the pseudomonas aeruginosa ectotoxin A, and can effectively neutralize the toxic effect of thepseudomonas aeruginosa ectotoxin A, thereby playing a role in protecting normal cells.

The invention discloses a strain of bacillus amyloliquefaciens with an inhibiting effect on fusarium oxysporum and application of the bacillus amyloliquefaciens. The fusarium oxysporum (FON) is pathogenic bacteria of watermelon fusarium wilt, and biological control is a crucial method for controlling the watermelon fusarium wilt. A bacterial strain for inhibiting the FON is screened from wheat rhizosphere, and the bacterial strain is Bacillus amyloliquefaciens LZN01 through authentication. Experimental results show that the antibacterial rate of the Bacillus amyloliquefaciens LZN01 to the FONis 57.07%, and the spore germination inhibition ratio of the Bacillus amyloliquefaciens LZN01 to the FON is 86.21%. Under the conditions that the temperature is 20-60 DEG C and the pH value is 4-9, the antibacterial effect of a fermented supernatant fluid of the Bacillus amyloliquefaciens LZN01 is stable, and antibacterial substances of the supernatant fluid contain Myriocin, Sphingofungin E, Sphingofungin F, Sphingofungin C and Gabapentin. Therefore, the Bacillus amyloliquefaciens LZN01 has a better inhibiting ability to the FON, the antibacterial effect is stable, and the development and application value is larger in biological control of the watermelon fusarium wilt.

Disclosed is a light source combination method. The light source combination method comprises the steps of (1) providing a plurality of light-emitting components, and driving the light-emitting components to give out multiple colored lights; (2) determining whether a Planck curve in a CIE coordinate graph is surrounded by multiple CIE coordinate positions corresponding to the multiple colored lights or not; if so, executing a step (3), and if not, executing the step (1) repeatedly; (3) mixing the multiple colored lights into one mixed light, wherein the mixed light has a specific CIE coordinate; (4) determining whether the specific CIE coordinate is positioned above the Planck curve in the CIE coordinate graph or not; if so, executing a step (6), and if not, executing a step (5); (5) driving the light-emitting components to adjust light intensity of one or more colored lights, and executing the step (3) repeatedly; and (6) performing further integration on the light-emitting components to form a light emitting unit or a light emitting module, wherein the light emitting unit or the light emitting module can give out a high-quality light source. By virtue of the method and the process, the light emitting unit which can give out the high-quality light source can be combined and manufactured by an engineer favorably.

The invention discloses substituted-pyrrolidinyl-contained thiomorpholine compounds, a preparation method thereof and applications thereof. In concrete, the invention relates to compounds having a general formula (I), stereoisomers of the compounds and pharmaceutical acceptable salts of the compounds, wherein R is shown in the specification. The invention also relates to pharmaceutical compositions comprising the compounds, and the applications of the compounds in preparing medicines used for treating and/or preventing a disease or a symptom which are related to over-high activity of DPP-IV or over-expression of the DPP-IV, and also relates to a method of using the compounds for treating related diseases. The compounds have activity for effectively inhibiting the DPP-IV.

The invention provides an application of echinacoside to preparation of a product for treating pruritus by inhibiting a transient receptor potential vanillin receptor 3 channel. The inhibiting effectof the echinacoside on the transient receptor potential vanillin receptor 3 channel is verified by a calcium fluorescence method and a manual patch clamp technique, and the median inhibitory concentration IC50 of the echinacoside for the transient receptor potential vanillin receptor 3 channel is 14.7+/-0.6 micrometers. The echinacoside is a specific inhibitor of the transient receptor potential vanillin receptor 3 channel. The anti-pruritus activity of the echinacoside is evaluated by a mouse skin pruritus model, and the echinacoside has an anti-pruritus effect on mouse acute pruritus causedby histamine. The echinacoside serving as the inhibitor of the transient receptor potential vanillin receptor 3 channel can be used for preparing the product for treating pruritus by inhibiting the transient receptor potential vanillin receptor 3 channel.

The invention discloses pulse stimulation equipment capable of effectively avoiding pacing in an atrial vulnerable period. The pulse stimulation equipment comprises an atrial sensing module, a pacing control module, an atrial pacing module, a clock/timing module and a storage module, the atrium sensing module senses and sends an atrium activity signal to the pace-making control module; when the pace-making control module recognizes an atrial activity signal as an atrial perception event in a non-stress period, the clock/timing module is controlled to time a T1 period and a T2 period which are stored in the storage module and adjacent in time sequence according to the T1 period and the T2 period which are stored in the storage module and adjacent in time sequence; the pacing control module inhibits the atrial pacing module from issuing an atrial pacing event when it is determined that the expected atrial pacing event occurs at the T2 period. The problem of atrial fibrillation danger caused by pacing in the atrial vulnerable period is solved.

The invention discloses a method for high-flux screening of drugs used for pseudorabies viruses. The method comprises the following steps of 1, inoculating a white optical-bottom 96-well cell plate with IBRS-2 cells, and carrying out culture in an incubator containing 5% of CO2 and having a temperature of 37 DEG C, 2, removing a growth solution, adding a maintenance media into the culture product, adding compounds needing to be screened into the culture product, orderly carrying out coubling dilution, carrying out uniform mixing, adding a pseudorabies virus Hubei A strain into each one of the wells of the white optical-bottom 96-well cell plate, and arranging a control group without the pseudorabies viruses and the compounds, and a control group with the pseudorabies viruses and without the compounds on the same white optical-bottom 96-well cell plate, wherein the compounds are dissolved in dimethylsulfoxide, 3, carrying out continue culture in the incubator containing 5% of CO2 and having a temperature of 37 DEG C, adding a Celltiterglo reagent into the culture, carrying out detection in a high-flux chemical luminescent detector, and calculating inhibition ratios of the compounds, and 4, utilizing the compound having a high inhibition rate as a potential drug for resisting pseudorabies viruses. The method is simple and fast, has a low cost, is convenient for high flux operation, can be used for screening aiming at the whole proliferation process of pseudorabies viruses in cells, and has a high success rate.

The invention discloses application of an aspartic protease gene in improvement of beauveria bassiana variety. The yield and toxicity of conidia of the beauveria bassiana are improved by knocking out the aspartic protease gene BbASP, or the high-temperature tolerance of the beauveria bassiana is improved by over-expressing the aspartic protease gene BbASP, and the nucleotide sequence of the aspartic protease gene BbASP is as shown in SEQ ID NO. 20; if the BbASP knockout strain is cultured for 10 d at 26 DEG C, the conidium yield is 72.57% higher than that of a wild type, if the BbASP knockout strain is cultured for 20 d at 26 DEG C, the conidium yield is 59.43% higher than that of a wild type, the survival rate of a host is reduced, the half lethal time is shortened, the time that entomogenous thalli penetrate out of the dead host is shortened, and the growth rate of the BbASP over-expression strain is increased under the high-temperature stress of 32 DEG C; and after the conidia are stressed at the high temperature of 43 DEG C for 2 h and 4 h, the germination rates are respectively increased by 7% and 11.34%.

The invention discloses preparation and application of a human serum albumin-NAMI-A ruthenium inorganic medicine compound. NAMI-A and the human serum albumin are incubated, the mixture is concentrated, the concentrated mixture is repeatedly washed by secondary deionized water till the DMSO content is smaller than 0.01 percent, then concentration is performed to obtain the human serum albumin-NAMI-A ruthenium inorganic medicine compound, and the compound is prepared into an injection, tablets, pills, capsules, a suspending agent or an emulsion to treat breast cancer, stomach cancer, lung cancer, colon cancer or liver cancer. The compound has high vascular permeability and retention rate, the medicine selectivity gathers in tumor cells while not influencing normal cells, the targeting of the ruthenium inorganic medicine is remarkably improved and the toxicity is greatly lowered.

The invention relates to a pyrrolopyrimidine ketone compound and a preparation method and application thereof, and belongs to the technical field of medicine. The pyrrolopyrimidine ketone compound having a structural feature of formula I, or a pharmaceutically acceptable salt thereof has a very good inhibition effect on DPP-IV, and almost no effect on activity of DPP-VIII and DPP-IX, can effectively control the blood glucose concentration of diabetic rats, and is safe and low in toxicity through the verification of pharmacological and toxicological tests. After being prepared into a medicine, the compound provided by the invention not only has an obvious efficacy but also has very small side effects, thus greatly improving the administration convenience and patient use compliance, therefore, the compound has obvious advantages compared with the same kind of medicine. The formula is shown in the specification.

The invention discloses sterilizing and acarus-killing compound essential oil. The essential oil comprises a solvent and essential oil. The essential oil comprises 0.02-0.04% of bergamot essential oil, 0.04-0.06% of lemon essential oil, 0.01-0.03% of rosemary essential oil, and 0.01-0.02% of pepper and mint essential oil; 0.01%-0.02% of eucalyptus essential oil; The solvent comprises the followingcomponents by mass: 8090% of water; 2.0-5.0% of a PPG-26-butanol polyether-26; 2.0-5.0% of propylene glycol; 1.0-2.0% of PEG-40 hydrogenated castor oil; 2.0-5.0% of 1, 2-hexanediol; 2.0-5.0% of 1, 3-propylene glycol; and 0.01-0.05% of capryloyl hydroxamic acid. The sterilizing and acarus-killing compound essential oil can effectively remove mites, has a good killing effect, is non-toxic and harmless, and has no side effects on the environment and human bodies.

The invention relates to a rice seed treatment agent, which comprises effective components and auxiliary agents, wherein the mass of the effective components accounts for 5-85% of the total mass of the rice seed treatment agent, the effective components are isoxadifen and gibberellin, and the auxiliary agents comprise three or more than three materials selected from a surfactant, a solvent, a suspending auxiliary agent and an anti-freezing agent. According to the present invention, the rice seed treatment agent can treat rice seeds, can improve the drug resistance on fenoxaprop-P-ethyl and other herbicides at the rice seedling stage, can improve the selectivity between rice and other malicious weeds such as weedy rice and the like of herbicides, can ensure the seedling emergence rate of rice, and can reduce the influence on the germination rate of rice seeds during the single treatment with isoxadifen.

The invention relates to a daphnoretin micelle and a preparation method, content detection and application thereof. The daphnoretin nano-micelle is prepared by selecting a safe and degradable material PEG-PLA, and daphnoretin is prepared into a polymer micelle so as to achieve the purposes of increasing solubility and prolonging in-vivo action time. Meanwhile, the glycyrrhetinic acid coupled PEG-PLA is used as a carrier, and the nano preparation which is loaded with daphnoretin and has the liver cancer targeting property is prepared. Through in-vitro cell research, in-vivo targeting evaluation and preliminary pharmacodynamic research on the two drug-loaded micelles, the liver targeting drug delivery performance and the anti-tumor effect of the daphnoretin nano-micelle drug system are proved, and a theoretical basis is laid for in-vivo liver cancer targeting research of daphnoretin in the future; and a new thought is provided for the development of a new daphnoretin dosage form and a novel liver cancer targeting preparation.

The invention provides a thienopyrimidinone compound or pharmaceutically-acceptable salt thereof and a preparation method and application thereof. The thienopyrimidinone compound or the pharmaceutically-acceptable salt thereof has a novel chemical structure, it is verified through in-vitro and in-vivo experiments that a very good selective inhibiting effect on DPP-IV is achieved, the activity of DPP-VIII and DPP-IX is barely affected while the activity of DPP-IV is effectively inhibited, meanwhile, the inhibition ratio of a potassium ion channel is low, and it can be predicted that the toxicity is low after the compound is developed into medicine. Compared with medicine trelagliptin orally taken once every week on the market, the thienopyrimidinone compound has fairly high or higher bioavailable efficiency, it can be predicted that after the compound is developed, the treatment effect that the compound is orally taken once for a long time, and the convenience and compliance of a patient are greatly improved. The preparation method is simple, the raw materials are easy to obtain, and the preparation method is suitable for industrial large-scale production.