49 results about "Sputum specimen" patented technology

The invention discloses a primer and a probe for quantitative determination of klebsiella pneumonia, and an application of the primer and the probe. Nucleotide sequences of the primer are a sequence 3 and a sequence 4 in a sequence table; a nucleotide sequence of the probe is a sequence 5 in the sequence table. The primer and the probe have the beneficial effects that the specific primer and probe sequences of the klebsiella pneumonia disclosed by the invention can be applied to qualitative and quantitative detection of the klebsiella pneumonia, the target of accurately and quantitatively determining the deoxyribonucleic acid (DNA) content of the klebsiella pneumonia in a specimen to be detected can be achieved by extracting the DNA in the sample to be detected and combining with a real-time fluorescence quantification polymerase chain reaction (PCR) detection technology, and the primer and the probe can be applied to food detection, scientific research and clinical diagnosis and used for carrying out qualitative and quantitative analysis on the klebsiella pneumonia DNA in samples such as a food sample, a suffer throat swab, nasopharyngeal secretion, people sputum specimens and blood samples. Thus, the primer and the probe have an important significance on judgment of klebsiella pneumonia infection, evaluation of treatment effectiveness and dynamic observation of the state of an illness, and simultaneously play an important role in the field of detection of clinical medicine.

The invention provides a sputum specimen homogenization method and preparation of a reagent for medical detection of a sputum specimen. The invention further provides a novel sputum digestion solution conforming to clinical detection, and the novel sputum digestion solution is prepared from 0.2% (w/v) of N-acetyl-L-cysteine, 0.1% (w/v) of trypsin, 0.4% (w/v) of triethanolamine, 0.2% (w/v) of potassium chloride, 1.5% (w/v) of potassium dihydrogen phosphate, 0.2% (w/v) of dipotassium hydrogen phosphate and a sterile water solvent.

The invention discloses a group of specific primers and a probe, which are designed and screened according to an IS6110 sequence of M. Tuberculosis (MTB), and a method for real-time fluorescent polymerase chain reaction (PCR) detection of DNA of the MTB by using the primers and the probe. A PCR reaction system comprises anti-pollution UNG enzyme, and belongs to the field of biotechnology. The real-time fluorescent PCR detection method established by the primers and the probe has high sensitivity and specificity, and particularly has higher tested positive rate for low MTB carried smear negative sputum specimens and blood samples than other similar detection methods.

A medical apparatus (FDA Classifications: “Sputum Specimen [In-Home] Self-Collection Device” and “In Vitro Medical Diagnostic Device”) and method for self-collection, transport, decontamination and storage preservation of expectorated sputum specimens, to optimize specimen quality and reliability of clinical laboratory stain interpretation, culture and molecular diagnostic tests, thereby improving laboratory support for pathogen-specific treatment (antibiotic stewardship).

The invention provides a sputum exfoliated cell preservation liquid with capacity of effectively improving a sputum specimen retention method and a slide preparation method as well as a preparation method of the sputum exfoliated cell preservation liquid. The sputum exfoliated cell preservation liquid is mainly prepared from polyethylene glycol-400 as a cell protecting agent, dithiothreitol as a mucus decomposer, ethyl alcohol as a cell stabilizer and the like. With the application of the sputum exfoliated cell preservation liquid, sputum can be preserved once spit, so that the sputum retention method is regulated, sputum collection time and storage time are prolonged, and cells in the sputum are prevented from autolysis and denaturation; meanwhile, the sputum treated with the preservation liquid can be applied to preparation of slides from liquid based thin-layer cells, therefore, the collection quantity of the cells is increased, the slide preparation effect is improved, and positive rate of sputum exfoliated cell detection is effectively increased. After application of the technology, sputum exfoliative cytologic examination can be simpler, more convenient, easier, safer and more economical and can be more easily popularized and used, and the sputum exfoliated cell preservation liquid and the preparation method thereof can be one of main methods for early diagnosis and screening of lung cancer, thereby effectively increasing early diagnosis rate and recovery rate of lung cancer and bringing benefits to humans.

The invention discloses sputum cell treatment liquid and an application thereof. The sputum cell treatment liquid comprises a solution A and a solution B, wherein the solution A is mainly prepared from polyethylene glycol-400 and ethanol in a mixing manner; tendril-leaved fritillary bulb and peeled black bean particles are also soaked into the solution A; the solution B is prepared from the following components in parts by weight: 0.1-0.2 part of 1,4-dimercapto-2,3-butanediol, 17-20 parts of sodium chloride, 7-10 parts of disodium hydrogen phosphate, 0.5-1.5 parts of sodium dihydrogen phosphate, 0.5-1.5 parts of thymol crystals, 8-12 parts of glacial acetic acid and 1900-2,000 parts of distilled water; the solution A and the solution B are separately stored; the solution A is firstly utilized to process a sputum specimen, and then the solution B is utilized to process the sputum specimen when the sputum cell treatment liquid is used. The sputum cell treatment liquid disclosed by the invention is simple in sheet fabrication method, high in sheet fabrication quality, good in detection effect, and low in cost, and can be applied to large-scale production.

The invention provides a method and a reagent for homogenizing a sputum specimen. The reagent is prepared from 0.2% (w/v) of N-acetyl-L-cysteine, 0.1% (w/v) of trypsin, 0.4% (w/v) of triethanolamine, 0.2% (w/v) of potassium chloride, 1.5% (w/v) of monopotassium phosphate, 0.2% (w/v) of dipotassium hydrogen phosphate and a sterile water solvent.

The invention discloses a method for fast screening Mycobacterium tuberculosis, comprising the following steps: firstly, liquefying specimens: adding liquefaction processing reagents in sputum specimens of patients and fully liquefying the sputum specimens; secondly, enriching and purifying specimens: coating specific antibodies of the Mycobacterium tuberculosis on a magnetic microballon, then adding the specific antibodies of the Mycobacterium tuberculosis in the liquefied specimens of patients, fully stirring the mixture to cause the mixture fully to be combined on an immune magnetic microballon and obtaining the well processed magnetic microballon to be tested after the washing of washing liquor; thirdly, foaming test: diluting and uniformly mixing the magnetic microballon to be tested using phosphate buffer solution to carry out the foaming test; if the specimens do not generate foams at room temperature, the specimens are negative tuberculosis; if the specimens generate foams at room temperature, and generate foams after being processed for 20 minutes at 68 DEG C after the foaming test, the specimens are negative; and if the specimens do not generate foams, the specimens are positive Mycobacterium tuberculosis. The whole detection process is simple, easily-operated, and highly precise and ensures the timely treatment of tuberculosis patients.

The invention discloses a nocardia asteroid selective medium for a sputum specimen, and belongs to the field of inspective microorganism culture. The nocardia asteroid selective medium is characterized in that 1000ml of the medium comprises beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, argutone, vitamin K1, sterile defibered sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the characteristics of reliable detection and high nocardia asteroid separation rate.

The invention relates to a sputum specimen preparation staining method which comprises the following steps: collecting a to-be-detected sputum specimen, mixing the sputum specimen and sputum treatmentliquid, and shaking to completely decompose the mixture; transferring the mixture into a test tube to perform oscillatory centrifugation after the sputum solution is completely decomposed, removing the supernatant, injecting cell preservation liquid into the test tube, and performing preparation by an automatic preparation staining machine, wherein the sputum treatment liquid comprises the following components in percentage by weight: 2-8% of phosphate, 2-3% of phenol, 2-3% of bromhexine, 20-40% of ethanol and 46-74% of distilled water. The sputum specimen preparation staining method providedby the invention is simple in process and small in usage amount of the sputum treatment liquid, and when the method is matched with the automatic preparation staining machine, qualified staining sections can be prepared on a large scale at a time and are stable in quality, and the single speed can reach 2 sections per minute.

A method for automated detection, monitoring and treatment of dysplasia by analyzing 3D reconstructed images of cells obtained from a specimen includes a biological specimen classifier that classifiescells from the sputum specimen as normal or abnormal. If abnormal cells are detected, then the abnormal cells are further classified as pre-cancerous or cancerous. Pre-cancerous cells are further sub-classified as being of glandular origin or squamous origin (dysplastic cells). This information would be used to define patient therapy so that if the cells are classified as dysplastic, then a cancer chemoprevention pharmaceutical like iloprost is administered to the subject over a predetermined time period to achieve a therapeutic dosage, and if only malignant cells were found or malignant andpre-cancerous cells were found, then next steps would involve patient triage to biopsy and surgery and possibly use of a cancer chemoprevention pharmaceutical.

The invention relates to a method for extracting small-molecular alpha-defensin polypeptide from sputum of a patient, which comprises the following steps: 1. collecting and preserving the sputum of the patient; 2. adding acetic acid into the sputum specimen, preparing a homogenate, stirring and leaching, centrifugating the homogenate, and taking the supernatant to prepare the crude extract; 3. adding DTT into the crude extract, and evenly mixing to obtain a mixture; 4. loading the mixture to a Pullulan gel column, eluting and purifying with acetic acid at normal temperature, preparing an elution curve, monitoring the collection of the eluate, sampling the extract, and carrying out mass spectrometric detection; 5. carrying out ultrafiltration concentration, carrying out vacuum freeze-drying, and storing; and 6. carrying out protein amino acid sequencing on the extract, and detecting the antimicrobial activity of the extract for Escherichia coli by using an agarose dispersion process. The method has the advantages of simple preparation process, low cost and high yield; the obtained product is natural alpha-defensin with high purity and high antimicrobial bioactivity; and the method provided by the invention is obviously better than the common chemical synthesis and recombinant protein method.

The invention discloses a multi-purpose sputum sample processing device, which includes a cup body, the upper end of the cup body is connected with a cup cover through the cup mouth, and the upper part of the cup cover is provided with a tapered cup mouth with a small upper part and a larger bottom part; The front-end internal pipeline of the tapered cup mouth has a filter screen, and the cup body is a flexible hose. The invention also discloses a multi-purpose sputum sample processing method, which includes the steps of collecting samples, processing samples, and distributing samples. The purpose of the present invention is to provide a multi-purpose sputum sample processing device, which can realize the requirement of one sample with multiple functions, and safely and effectively complete different tests on the same sample.

A sputum sample collection device, the pressure reducer is connected to the sputum storage bottle, the sputum storage bottle is connected to the device tube, the device tube is connected to the connecting tube, the connecting tube is connected to the electric suction device, the pressure reducer is connected to the nipple connecting tube, and the nipple connecting tube is connected to the sputum suction tube, This technology greatly reduces the frequency of damage to the respiratory mucosa of patients and reduces the work pressure of medical staff.

The invention discloses a sputum cup with reminding and timing functions. The sputum cup comprises a sputum cup body and a cup cover, wherein a bottom of the sputum cup body is a thin film and is in aloose state in a natural state, a connecting body is arranged at the bottom of the sputum cup body, and the connecting body is buckled with a reminding device; the reminding device includes a base block, one side of the base block is sequentially provided with a gravity sensor, a partition groove and a connecting groove from the center to the outside, a spring is arranged in the connecting groove, one end of the spring is fixedly connected with the bottom of the connecting groove, the other end of the spring is fixedly connected with a backing ring, connecting columns are symmetrically arranged in the connecting groove of the base block in a direction from the middle to two sides, a timer is embedded in one side of the base block and the connecting body is provided with a clamping groovematched with the connecting columns; and the gravity sensor is connected with the timer, and the gravity sensor is wirelessly connected with an external alarm device. According to the sputum cup, waiting time of a medical staff is shortened, a problem that sampling is not timely is avoided, sampling time is automatically recorded, a qualification of sputum specimens is conveniently judged, the reminding device can be recycled, and resource wastes are avoided.

The invention provides a sputum suction pipe. The sputum suction pipe comprises a sputum suction hose provided with an inlet and an outlet. The sputum suction pipe is characterized in that the sputum suction hose is provided with two protruding interfaces, one interface is connected with an inflation device in an inserted mode, the other interface is connected with a transparent liquid storage cavity in an inserted mode, and an exhaust hole is formed in the liquid storage cavity. A clamp is installed between the inlet and the interface nearest to the inlet, and a clamp is installed between the outlet and the interface nearest to the outlet. The sputum suction pipe is matched with opening and closing of the clamps so as to be used for sputum suction, a sputum specimen can be conveniently obtained, the structure is simple, and time and labor are saved during operation.

The pretreating liquid includes initial treating liquid and cracking liquid. The initial treating liquid is NaOH solution of 2-6 mol/L concentration. The cracking liquid consists of NP40, Tris-HCL, NaCl and MgCl2. The use of the pretreating liquid makes it possible for direct hybridization by use of DNA probe without PCR before horseradish peroxidase catalyzed reaction, such as chemical luminous self-developing detection. The present invention can obtain high sensitivity and high specificity and has simple operation, low cost and wide use.