49 results about "Sputum specimen" patented technology

The invention discloses a primer and a probe for quantitative determination of klebsiella pneumonia, and an application of the primer and the probe. Nucleotide sequences of the primer are a sequence 3 and a sequence 4 in a sequence table; a nucleotide sequence of the probe is a sequence 5 in the sequence table. The primer and the probe have the beneficial effects that the specific primer and probe sequences of the klebsiella pneumonia disclosed by the invention can be applied to qualitative and quantitative detection of the klebsiella pneumonia, the target of accurately and quantitatively determining the deoxyribonucleic acid (DNA) content of the klebsiella pneumonia in a specimen to be detected can be achieved by extracting the DNA in the sample to be detected and combining with a real-time fluorescence quantification polymerase chain reaction (PCR) detection technology, and the primer and the probe can be applied to food detection, scientific research and clinical diagnosis and used for carrying out qualitative and quantitative analysis on the klebsiella pneumonia DNA in samples such as a food sample, a suffer throat swab, nasopharyngeal secretion, people sputum specimens and blood samples. Thus, the primer and the probe have an important significance on judgment of klebsiella pneumonia infection, evaluation of treatment effectiveness and dynamic observation of the state of an illness, and simultaneously play an important role in the field of detection of clinical medicine.

The invention provides a sputum specimen homogenization method and preparation of a reagent for medical detection of a sputum specimen. The invention further provides a novel sputum digestion solution conforming to clinical detection, and the novel sputum digestion solution is prepared from 0.2% (w / v) of N-acetyl-L-cysteine, 0.1% (w / v) of trypsin, 0.4% (w / v) of triethanolamine, 0.2% (w / v) of potassium chloride, 1.5% (w / v) of potassium dihydrogen phosphate, 0.2% (w / v) of dipotassium hydrogen phosphate and a sterile water solvent.

The invention discloses a group of specific primers and a probe, which are designed and screened according to an IS6110 sequence of M. Tuberculosis (MTB), and a method for real-time fluorescent polymerase chain reaction (PCR) detection of DNA of the MTB by using the primers and the probe. A PCR reaction system comprises anti-pollution UNG enzyme, and belongs to the field of biotechnology. The real-time fluorescent PCR detection method established by the primers and the probe has high sensitivity and specificity, and particularly has higher tested positive rate for low MTB carried smear negative sputum specimens and blood samples than other similar detection methods.

A medical apparatus (FDA Classifications: “Sputum Specimen [In-Home] Self-Collection Device” and “In Vitro Medical Diagnostic Device”) and method for self-collection, transport, decontamination and storage preservation of expectorated sputum specimens, to optimize specimen quality and reliability of clinical laboratory stain interpretation, culture and molecular diagnostic tests, thereby improving laboratory support for pathogen-specific treatment (antibiotic stewardship).

The invention discloses an oscillator for uniformly mixing a sputum specimen and an operating method of the oscillator. The oscillator comprises a stand and a container which is arranged right above the stand, wherein a motor is arranged in the stand, the motor is connected with a central rotating shaft, the central rotating shaft is connected with the container, a supporting device is arranged outside the central rotating shaft, the supporting device is distributed between the stand and the container by adopting the central rotating shaft as the center, and the supporting device is respectively connected with the stand and the container; a reagent bottle is arranged in the container, a supporting plate is driven by the rotation of the motor to enable the container to circumferentially rotate, so that the container makes plane reciprocating vibration in operation, and the sputum specimen in the reagent bottle can be uniformly mixed. By adopting the oscillator and the operating method thereof, the labor intensity can be greatly alleviated, the uniformly mixed reagent which is obtained in mass production has the excellent characteristics that the uniformity in mixing is good, the flow standard is uniform, microorganisms in the reagent after the reagent is added into a heating system are adequately and uniformly mixed under an environment with the temperature being identical to the human body temperature, and the like.

The invention provides a sputum exfoliated cell preservation liquid with capacity of effectively improving a sputum specimen retention method and a slide preparation method as well as a preparation method of the sputum exfoliated cell preservation liquid. The sputum exfoliated cell preservation liquid is mainly prepared from polyethylene glycol-400 as a cell protecting agent, dithiothreitol as a mucus decomposer, ethyl alcohol as a cell stabilizer and the like. With the application of the sputum exfoliated cell preservation liquid, sputum can be preserved once spit, so that the sputum retention method is regulated, sputum collection time and storage time are prolonged, and cells in the sputum are prevented from autolysis and denaturation; meanwhile, the sputum treated with the preservation liquid can be applied to preparation of slides from liquid based thin-layer cells, therefore, the collection quantity of the cells is increased, the slide preparation effect is improved, and positive rate of sputum exfoliated cell detection is effectively increased. After application of the technology, sputum exfoliative cytologic examination can be simpler, more convenient, easier, safer and more economical and can be more easily popularized and used, and the sputum exfoliated cell preservation liquid and the preparation method thereof can be one of main methods for early diagnosis and screening of lung cancer, thereby effectively increasing early diagnosis rate and recovery rate of lung cancer and bringing benefits to humans.

The invention discloses sputum cell treatment liquid and an application thereof. The sputum cell treatment liquid comprises a solution A and a solution B, wherein the solution A is mainly prepared from polyethylene glycol-400 and ethanol in a mixing manner; tendril-leaved fritillary bulb and peeled black bean particles are also soaked into the solution A; the solution B is prepared from the following components in parts by weight: 0.1-0.2 part of 1,4-dimercapto-2,3-butanediol, 17-20 parts of sodium chloride, 7-10 parts of disodium hydrogen phosphate, 0.5-1.5 parts of sodium dihydrogen phosphate, 0.5-1.5 parts of thymol crystals, 8-12 parts of glacial acetic acid and 1900-2,000 parts of distilled water; the solution A and the solution B are separately stored; the solution A is firstly utilized to process a sputum specimen, and then the solution B is utilized to process the sputum specimen when the sputum cell treatment liquid is used. The sputum cell treatment liquid disclosed by the invention is simple in sheet fabrication method, high in sheet fabrication quality, good in detection effect, and low in cost, and can be applied to large-scale production.

The invention discloses an automatic sputum specimen smearing instrument. The automatic sputum specimen smearing instrument comprises a shell and a controller, wherein a glass slide accommodating chamber, a smearing chamber and a staining chamber are arranged inside the shell from the center to the edge in sequence, a slide outlet chamber is arranged outside the shell, a glass slide accommodating disc is arranged in the glass slide accommodating chamber, and a first lifting device is arranged between the bottom of the glass slide accommodating disc and the shell; an annular accommodating disc and a smearing device are arranged in the smearing chamber, a staining fluid bottle, a water pipe, a staining fluid collecting box and a drying device are arranged in the staining chamber, and a first pair of sliding rails, a second pair of sliding rails, a third pair of sliding rails and a fourth pair of sliding rails matched with one another are arranged in the glass slide accommodating chamber, the smearing chamber, the staining chamber and the slide outlet chamber respectively. By the adoption of the automatic sputum specimen smearing instrument, smearing, staining, drying and cleaning of a sputum specimen can be achieved automatically, operation is easy and convenient, and time is saved; opening and closing of a bottle cap and smearing are achieved through a smearing device, operation is easy and convenient, and cost is saved.

The invention discloses a sputum pot with a smearing function. The sputum pot comprises a small cover, a duck-mouth type large cover, a bottle body, a ball and an elastic bracket, wherein the small cover is detachably connected to the top of the duck-mouth type large cover in a sealing manner; the bottom of the duck-mouth type large cover is detachably connected to an opening of the bottle body in a sealing manner; a flat mouth part with an inner hole which gradually becomes small is arranged at the top of the duck-mouth type large cover; a coating surface with a liquid outlet is arranged on the top end of the mouth part; the elastic bracket is mounted in the mouth part; a concave pit is formed in the top of the elastic bracket; the bottom of the ball is supported by the concave pit; one small part of the ball is exposed out of the liquid outlet of the coating surface; the inner diameter of the liquid outlet is smaller than the diameter of the ball. On one hand, the efficiency of homogenized smearing is improved and the risk of contacting a sputum specimen by an operator is reduced; on the other hand, the amount of the smeared sputum specimens in each time is in a certain range and the smearing is uniform so that the smearing operation can easily realize standardization, the random errors are reduced and the production efficiency is improved.

The invention provides a method and a reagent for homogenizing a sputum specimen. The reagent is prepared from 0.2% (w / v) of N-acetyl-L-cysteine, 0.1% (w / v) of trypsin, 0.4% (w / v) of triethanolamine, 0.2% (w / v) of potassium chloride, 1.5% (w / v) of monopotassium phosphate, 0.2% (w / v) of dipotassium hydrogen phosphate and a sterile water solvent.

The invention discloses an anti-splash sputum collecting device, and belongs to the technical field of medical items. The anti-splash sputum collecting device comprises a face mask, a sputum collecting cup, a first sleeve and a second sleeve; a flow-guiding tube is arranged in a position, corresponding to a mouth, on the face mask; the sputum collecting cup is connected with the bottom end of theflow-guiding tube; and the flow-guiding tube is sleeved with the top ends of both the first sleeve and the second sleeve, the top ends of the first sleeve and the second sleeve are connected with an outer side wall of the flow-guiding tube separately, and drawstrings are arranged at the bottom ends of the first sleeve and the second sleeve respectively. When a sputum specimen is collected by the anti-splash sputum collecting device, the face of a patient is covered with the face mask, after collection is completed, the first sleeve is turned up so that the face mask can be sleeved with the first sleeve, sealing is achieved through the corresponding drawstring, an opening in the bottom end of the flow-guiding tube is sleeved with the second sleeve, and sealing is achieved through the corresponding drawstring, so that the situation that in a collection process and after collection is completed, droplets, aerosols and the like from an airway of a patient float in the air to cause virus infection is avoided.

The invention discloses a sputum pretreatment method for quickly carrying out nucleic acid detection. The sputum pretreatment method comprises the following steps of a first step, adding a NaOH solution with a concentration of 4 to 30 percent into a collected sputum specimen, wherein the amount of the NaOH solution is 4 times that of the collected sputum specimen, carrying out vortex oscillation to liquefy the collected sputum specimen, and standing for 5min to 10min at a room temperature; a second step, adding a citric acid solution with a concentration of 20 to 80 percent into specimen liquid treated in the first step, and uniformly oscillating and mixing; a third step, adding thiourea with a concentration of 5 to 14 percent into specimen liquid treated in the second step, uniformly oscillating and mixing, then standing at the room temperature, and removing a deposit. The sputum pretreatment method has the advantages that the method is simple and quick, as the entire pretreatment process does not need centrifugation, the operation is convenient, and the consumed time is shorter (less than 15 minutes); the specimen liquid pretreated by the method provided by the invention is higher in sensitivity; 30 bacteria per milliliter can be lowest detected; the specificity is good, and according to a clinical specimen verification result, the negative coincidence rate can reach 100 percent.

The invention discloses a method for fast screening Mycobacterium tuberculosis, comprising the following steps: firstly, liquefying specimens: adding liquefaction processing reagents in sputum specimens of patients and fully liquefying the sputum specimens; secondly, enriching and purifying specimens: coating specific antibodies of the Mycobacterium tuberculosis on a magnetic microballon, then adding the specific antibodies of the Mycobacterium tuberculosis in the liquefied specimens of patients, fully stirring the mixture to cause the mixture fully to be combined on an immune magnetic microballon and obtaining the well processed magnetic microballon to be tested after the washing of washing liquor; thirdly, foaming test: diluting and uniformly mixing the magnetic microballon to be tested using phosphate buffer solution to carry out the foaming test; if the specimens do not generate foams at room temperature, the specimens are negative tuberculosis; if the specimens generate foams at room temperature, and generate foams after being processed for 20 minutes at 68 DEG C after the foaming test, the specimens are negative; and if the specimens do not generate foams, the specimens are positive Mycobacterium tuberculosis. The whole detection process is simple, easily-operated, and highly precise and ensures the timely treatment of tuberculosis patients.

The invention discloses a nocardia asteroid selective medium for a sputum specimen, and belongs to the field of inspective microorganism culture. The nocardia asteroid selective medium is characterized in that 1000ml of the medium comprises beef liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferroporphyrin, calcium lactate, nalidixic acid, argutone, vitamin K1, sterile defibered sheep blood and the balance of distilled water. Compared with the prior art, the nocardia asteroid selective medium has the characteristics of reliable detection and high nocardia asteroid separation rate.

The invention relates to a sputum specimen preparation staining method which comprises the following steps: collecting a to-be-detected sputum specimen, mixing the sputum specimen and sputum treatmentliquid, and shaking to completely decompose the mixture; transferring the mixture into a test tube to perform oscillatory centrifugation after the sputum solution is completely decomposed, removing the supernatant, injecting cell preservation liquid into the test tube, and performing preparation by an automatic preparation staining machine, wherein the sputum treatment liquid comprises the following components in percentage by weight: 2-8% of phosphate, 2-3% of phenol, 2-3% of bromhexine, 20-40% of ethanol and 46-74% of distilled water. The sputum specimen preparation staining method providedby the invention is simple in process and small in usage amount of the sputum treatment liquid, and when the method is matched with the automatic preparation staining machine, qualified staining sections can be prepared on a large scale at a time and are stable in quality, and the single speed can reach 2 sections per minute.

A method for automated detection, monitoring and treatment of dysplasia by analyzing 3D reconstructed images of cells obtained from a specimen includes a biological specimen classifier that classifiescells from the sputum specimen as normal or abnormal. If abnormal cells are detected, then the abnormal cells are further classified as pre-cancerous or cancerous. Pre-cancerous cells are further sub-classified as being of glandular origin or squamous origin (dysplastic cells). This information would be used to define patient therapy so that if the cells are classified as dysplastic, then a cancer chemoprevention pharmaceutical like iloprost is administered to the subject over a predetermined time period to achieve a therapeutic dosage, and if only malignant cells were found or malignant andpre-cancerous cells were found, then next steps would involve patient triage to biopsy and surgery and possibly use of a cancer chemoprevention pharmaceutical.

The invention relates to a method for extracting small-molecular alpha-defensin polypeptide from sputum of a patient, which comprises the following steps: 1. collecting and preserving the sputum of the patient; 2. adding acetic acid into the sputum specimen, preparing a homogenate, stirring and leaching, centrifugating the homogenate, and taking the supernatant to prepare the crude extract; 3. adding DTT into the crude extract, and evenly mixing to obtain a mixture; 4. loading the mixture to a Pullulan gel column, eluting and purifying with acetic acid at normal temperature, preparing an elution curve, monitoring the collection of the eluate, sampling the extract, and carrying out mass spectrometric detection; 5. carrying out ultrafiltration concentration, carrying out vacuum freeze-drying, and storing; and 6. carrying out protein amino acid sequencing on the extract, and detecting the antimicrobial activity of the extract for Escherichia coli by using an agarose dispersion process. The method has the advantages of simple preparation process, low cost and high yield; the obtained product is natural alpha-defensin with high purity and high antimicrobial bioactivity; and the method provided by the invention is obviously better than the common chemical synthesis and recombinant protein method.

The invention relates to the technical field of biology, in particular to a preserving solution for sputum and a preparation method. The preserving solution of each 1000ML comprises the following components: 0.5-3.5g of dithiothreitol, 5.0-30g of tris(hydroxymethyl)methyl aminomethane, 0.1-10.5g of sodium chloride, 0.01-2.0g of phosphorus trichloride, 0.01-2.5g of disodium hydrogen phosphate, 0.01-2.5g of potassium dihydrogen phosphate, 0.2-3.5g of guanidinium isothiocyanate and the balance nucleic acid-free water, and the pH value is 5.5-8.5. The preserving solution prepared by the inventioncan be stored and applied at room temperature, has a digesting / liquefying function, can effectively digest / liquefy specimens (sputum), does not need to digest the specimens before detection, and meanwhile, has an inactivation effect on specimen (sputum) viruses, so that the biosafety risk of specimen preservation, transportation, processing and detection is lowered; after being collected, sputum specimens collected by using the preserving solution can be stored for 72 hours at 2-8 DEG C, and can be stored for 10 days or more at ultra-low temperature (-70 DEG C); and moreover, the components ofthe preserving solution are easy to obtain, and the preparation cost is low.

The invention discloses a multifunctional sputum sample treatment device. The multifunctional sputum sample treatment device comprises a cup body, wherein the upper end of the cup body is connected with a cup cover through a cup mouth; the conical cup mouth is arranged at the upper part of the cup cover and is provided with a small upper part and a large lower part; a filter net is arranged in a pipeline in the front end of the conical cup mouth; the cup body is a telescopic flexible pipe. The invention also discloses a multifunctional sputum sample treatment method. The multifunctional sputumsample treatment method comprises the following steps of collecting a sample, treating the sample, distributing the sample, and the like. The multifunctional sputum sample treatment device has the advantages that the effect of one sample with multiple functions is realized, and the effect of one sample safely and effectively meeting different detection requirements is realized.

The invention provides a sputum specimen treatment solution which comprises the following components in percentage by weight: 2-8% of phosphate, 2-3% of phenol, 2-3% of bromhexine, 20-40% of ethanol and 46-74% of distilled water. The preparation method comprises the following steps: dissolving weighed phosphate into redistilled water, sequentially adding an ethanol solvent, phenol and bromhexine into the solution, and uniformly mixing, thereby obtaining the product. The sputum specimen treatment solution contains the sterilizing agent phenol, the polysaccharide cellulose decomposing agent bromhexine and the ethanol capable of achieving effects of disinfecting and dissolving, has the advantages of stable property, low cost and the like and has excellent competitive advantages compared withlike products in the market.

A sputum sample collection device, the pressure reducer is connected to the sputum storage bottle, the sputum storage bottle is connected to the device tube, the device tube is connected to the connecting tube, the connecting tube is connected to the electric suction device, the pressure reducer is connected to the nipple connecting tube, and the nipple connecting tube is connected to the sputum suction tube, This technology greatly reduces the frequency of damage to the respiratory mucosa of patients and reduces the work pressure of medical staff.

The invention discloses a sputum cup with reminding and timing functions. The sputum cup comprises a sputum cup body and a cup cover, wherein a bottom of the sputum cup body is a thin film and is in aloose state in a natural state, a connecting body is arranged at the bottom of the sputum cup body, and the connecting body is buckled with a reminding device; the reminding device includes a base block, one side of the base block is sequentially provided with a gravity sensor, a partition groove and a connecting groove from the center to the outside, a spring is arranged in the connecting groove, one end of the spring is fixedly connected with the bottom of the connecting groove, the other end of the spring is fixedly connected with a backing ring, connecting columns are symmetrically arranged in the connecting groove of the base block in a direction from the middle to two sides, a timer is embedded in one side of the base block and the connecting body is provided with a clamping groovematched with the connecting columns; and the gravity sensor is connected with the timer, and the gravity sensor is wirelessly connected with an external alarm device. According to the sputum cup, waiting time of a medical staff is shortened, a problem that sampling is not timely is avoided, sampling time is automatically recorded, a qualification of sputum specimens is conveniently judged, the reminding device can be recycled, and resource wastes are avoided.

The invention discloses a method for making an earthworm specimen. The preparation method comprises the following steps: after pre-cultivating the earthworms, soaking them in ethanol solution, filling and fixing the soaked earthworms with pentanediol, and sealing them with a preservation solution, etc. The method materials adopted in the invention are easy to obtain and do not contain toxic substances such as toluene, are environmentally friendly and safe, can maintain the original physiological structure and appearance of earthworms, have a simple manufacturing process, and are suitable for popularization.

The invention discloses a detachable liquid-collecting type sputum suction device, which comprises a liquid-collecting bottle, a negative-pressure suction tube and a collecting tube. The negative-pressure suction tube is connected with the negative-pressure device and the liquid-collecting bottle. The suction port of the bronchoscope is connected, and the liquid collection bottle includes an upper cover, a lower cover, a collection bottle and a sampling test tube; Semi-closed type, the cavity between the outer bottle and the isolator is sealed at the lower ends of the two, and the unsealed space at the lower end of the outer bottle is used for the passage of the sampling test tube; the sampling test tube is nested in the collection bottle The inside of the isolation body, the lower end of the sampling test tube is sealed, and the outer wall of the lower end of the sampling test tube is attached to the lower end of the outer bottle; Cap the test tube connected to the sampling test tube. The invention integrates common sputum suction and sample collection, and achieves the purpose of collecting sputum samples accurately without pollution.

The invention discloses a sputum pot with a smearing function. The sputum pot comprises a small cover, a duck-mouth type large cover, a bottle body, a ball and an elastic bracket, wherein the small cover is detachably connected to the top of the duck-mouth type large cover in a sealing manner; the bottom of the duck-mouth type large cover is detachably connected to an opening of the bottle body in a sealing manner; a flat mouth part with an inner hole which gradually becomes small is arranged at the top of the duck-mouth type large cover; a coating surface with a liquid outlet is arranged on the top end of the mouth part; the elastic bracket is mounted in the mouth part; a concave pit is formed in the top of the elastic bracket; the bottom of the ball is supported by the concave pit; one small part of the ball is exposed out of the liquid outlet of the coating surface; the inner diameter of the liquid outlet is smaller than the diameter of the ball. On one hand, the efficiency of homogenized smearing is improved and the risk of contacting a sputum specimen by an operator is reduced; on the other hand, the amount of the smeared sputum specimens in each time is in a certain range and the smearing is uniform so that the smearing operation can easily realize standardization, the random errors are reduced and the production efficiency is improved.

The invention discloses an automatic sputum specimen smearing instrument. The automatic sputum specimen smearing instrument comprises a shell and a controller, wherein a glass slide accommodating chamber, a smearing chamber and a staining chamber are arranged inside the shell from the center to the edge in sequence, a slide outlet chamber is arranged outside the shell, a glass slide accommodating disc is arranged in the glass slide accommodating chamber, and a first lifting device is arranged between the bottom of the glass slide accommodating disc and the shell; an annular accommodating disc and a smearing device are arranged in the smearing chamber, a staining fluid bottle, a water pipe, a staining fluid collecting box and a drying device are arranged in the staining chamber, and a first pair of sliding rails, a second pair of sliding rails, a third pair of sliding rails and a fourth pair of sliding rails matched with one another are arranged in the glass slide accommodating chamber, the smearing chamber, the staining chamber and the slide outlet chamber respectively. By the adoption of the automatic sputum specimen smearing instrument, smearing, staining, drying and cleaning of a sputum specimen can be achieved automatically, operation is easy and convenient, and time is saved; opening and closing of a bottle cap and smearing are achieved through a smearing device, operation is easy and convenient, and cost is saved.

The invention provides a sputum suction pipe. The sputum suction pipe comprises a sputum suction hose provided with an inlet and an outlet. The sputum suction pipe is characterized in that the sputum suction hose is provided with two protruding interfaces, one interface is connected with an inflation device in an inserted mode, the other interface is connected with a transparent liquid storage cavity in an inserted mode, and an exhaust hole is formed in the liquid storage cavity. A clamp is installed between the inlet and the interface nearest to the inlet, and a clamp is installed between the outlet and the interface nearest to the outlet. The sputum suction pipe is matched with opening and closing of the clamps so as to be used for sputum suction, a sputum specimen can be conveniently obtained, the structure is simple, and time and labor are saved during operation.

The invention discloses a centrifugal sputum case for induced sputum tubercle bacillus detection and belongs to the technical field of medical treatment detecting appliances. An existing sputum case is small in size and cannot be sealed, the sputum liquid is easy to leak outside so as to lead to tubercle bacillus dispersion and affect the detection effect of tuberculosis; the centrifugal sputum case disclosed by the invention can overcome these problems. The centrifugal sputum case comprises a sputum remaining cup, a sputum storage pipe body, a cock base, an inner pipe device and a sputum storage pipe cap, wherein the sputum storage pipe body is an integral test pipe comprising a conical top and a cylindrical bottom part which are in circular arc transition connection; the circular arc transition part of the inner wall of the sputum storage pipe body is provided with the cock base; the sputum remaining cup is installed in an annular groove at the inner side of the cock base; the cock base is also provided with the inner pipe device; the sputum storage pipe cap can directly cover the sputum storage pipe. The centrifugal sputum case is scientific and reasonable in structure, is low in cost, is simple, convenient and rapid to operate, is high in practicability, integrates sputum remaining and detection, shortens the sputum remaining time greatly, enhances the detection efficiency, reduces the re-pollution rate of a sputum specimen, and reduces the probability of infecting workers with tuberculosis.

The invention discloses a preparation method of a secretory type Rpf factor based on a transformant and application thereof. On the basis of constructing an exogenous expression vector pPIC-Rpf, the exogenous expression vector is converted into Pichia methanolica to prepare a recombinant, and the Pichia methanolica recombinant of a secretory type Rpf protein is obtained by resistance screening. APichia methanolica expression system can persistently secrete the Rpf protein with biologic activity; the protein can promote the resuscitation of mycobacterium smegmatis dormant bacteria and the growth of Mycobacterium tuberculosis (MTB) H37Ra, promotes the growth of the MTB in the sputum specimens of clinical TB (tuberculosis) patients and can be used for the rapid diagnosis of clinical TB.

The pretreating liquid includes initial treating liquid and cracking liquid. The initial treating liquid is NaOH solution of 2-6 mol / L concentration. The cracking liquid consists of NP40, Tris-HCL, NaCl and MgCl2. The use of the pretreating liquid makes it possible for direct hybridization by use of DNA probe without PCR before horseradish peroxidase catalyzed reaction, such as chemical luminous self-developing detection. The present invention can obtain high sensitivity and high specificity and has simple operation, low cost and wide use.