79 results about "Sputum sample" patented technology

The invention discloses a preparation method of Au (at) Au (at) Ag / Pt / M NPs and application of the Au (at) Au (at) Ag / Pt / M NPs in klebsiella pneumoniae detection, and belongs to the technical field of analysis and detection. The invention particularly relates to development of a signal probe and combination and assembly of the signal probe and lateral flow immunochromatography (LFIA) for klebsiella pneumoniae detection. Au (at) Au (at) Ag / Pt / MNPs is a multi-element noble metal nanoparticle with plasma resonance property, peroxidase-like activity and light stability, shows strong detection potential in three modes of colorimetric (CM), catalytic colorimetric (CL) and surface enhanced Raman scattering (SERS), and is loaded into LFIA to construct a multi-mode LFIA (CM / CL / SERS-LFIA). The kit is expected to be used for direct multimode detection of klebsiella pneumoniae in clinical sputum samples, and a new early detection strategy is provided for respiratory tract infection diseases such as klebsiella pneumoniae.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The utility model discloses an integrally formed sputum collector, which belongs to the technical field of medical instruments and comprises a collector, a valve body component and a drainage pipe fitting, the front end of the valve body component is communicated with a first suction tube, the tail end of the valve body component is communicated with a second suction tube, the drainage pipe fitting is arranged between the sputum collector and the valve body component, and the first suction tube is communicated with the first suction tube. Through the cooperation between the valve body assembly and the drainage pipe fitting, the communication state between the collector and the bronchofiberscope and the communication state between the suction device and the bronchofiberscope can be adjusted and controlled at any time, it is not needed that sampling is conducted again after the suction device sucks and collects sputum, and in the working process of the bronchofiberscope, sampling is conducted again. Sputum is sampled by the collector in a negative pressure state formed by the suction treatment of the suction device, so that a sputum collection sample independently collected in the suction device can be inspected, the sputum sample does not need to be obtained from a collection part in the suction device, the operation steps are reduced, and the detection efficiency is improved. And convenience is provided for surgical operation of medical personnel.

The present invention relates to a sputum sample microbial flora and nucleic acid DNA preservation solution, including the following components by mass: 0.5-2 parts of trishydroxymethylaminomethane, 0.2-1 parts of mucus digestive agent, 3-6 parts of N-octylpyridinium bromide, 20-50 parts of fixative, 0.6-1.5 parts of auxiliary fixative, 1-2 parts of metal ion chelating agent, 0.5-3 parts of inorganic salt, the metal ion chelating agent includes at least 50wt% of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; the auxiliary fixative is selected from at least one of sodium citrate and potassium citrate. The sputum sample microbial flora and nucleic acid DNA preservation solution using the technical solution of the present invention has the following advantages, can be stored and transported at room temperature for a long time, and is easy to operate; the sputum sample can be effectively liquefied by immersing the sputum sample in the protective solution, and the structural composition of the microorganism is stabilized at room temperature, protecting the diversity of its flora structure and DNA stability.

The invention relates to the technical field of component detection, in particular to a critical patient respiratory tract sputum component rapid detection system based on mass spectrum, which can realize the following steps through mutual cooperation of a plurality of modules: obtaining a target mass spectrum corresponding to a respiratory tract sputum sample of a patient to be detected, identifying a target characteristic peak in the target mass spectrum and an isotope peak cluster corresponding to the target characteristic peak; determining pathogen conformance indexes, protein flooding possibility and isotope peak distribution rationality corresponding to each target characteristic peak; screening out protein submerging characteristic peaks from all the target characteristic peaks; and segmenting a target pathogen peak from the protein submerging characteristic peak, and carrying out respiratory tract sputum component detection according to the target characteristic peak and the segmented target pathogen peak. By analyzing the protein submerging condition in the characteristic peak, the segmentation of the protein submerging characteristic peak is realized, so that the identification of the submerged pathogen is facilitated, and the accuracy of detecting the components of the sputum in the respiratory tract is further improved.

The three-dimensional propaganda and education model comprises a model main body, an identification assembly and a supporting base, the model main body is made of a transparent material, and an unqualified sputum specimen simulation unit (such as saliva and a polluted sputum specimen) and a qualified sputum specimen simulation unit (such as white viscous sputum, yellow viscous sputum and bloody sputum) are arranged in the model main body; each unit is of an independent cavity structure, and the sputum character is visually displayed by accurately controlling the color and viscosity and simulating particles (such as squamous epithelial cells or inflammatory cells); the identification assembly is used for clearly marking the type and the qualified standard through an identification board and a fluorescent coating; the supporting base provides stable placement. Through materialized comparison, the patient can quickly distinguish the sputum type, so that the retention quality is improved from the source, the percent of pass is remarkably improved, the nursing load is reduced, and the method has the advantages of intuition, standardization, high efficiency, safety and the like.

The invention discloses a severe pulmonary infection sputum sample sampling device, and relates to the technical field of medical sampling instruments.The severe pulmonary infection sputum sample sampling device comprises a sampling tank and a negative pressure pipe, a mounting cabin is fixedly installed on the sampling tank, a filtering module used for filtering sputum sample particles is further arranged outside the sampling tank, and a detection module used for detecting a sputum sample is further arranged on the sampling tank; the sampling tank is connected with the detection module through a transfer pipe, the filtering module comprises a filtering box installed on the mounting cabin in a sealed mode, a sampling pipe used for sucking a sputum sample is arranged on the filtering box, a sputum suction head is fixedly installed at the end, extending out of the filtering box, of the sampling pipe, and filtering holes distributed in an arrayed mode are formed in the side, close to the sputum suction head, of the filtering box. The filter holes are formed in the filter box, and the filter membrane is arranged at the joint of the sampling tank and the mounting cabin, so that secondary filtration of a sputum sample is realized, large particles and fine impurities in the sputum sample can be effectively removed, and a pure sample can be obtained without additional filtration steps.

The present application relates to the technical field of component detection, in particular to a rapid detection system for respiratory tract sputum components of severe patients based on mass spectrometry. The system can realize the following steps through the cooperation between multiple modules: obtaining a target mass spectrum corresponding to a respiratory tract sputum sample of a patient to be detected, and identifying a target characteristic peak and its corresponding isotope peak cluster in the target mass spectrum; determining a pathogen compliance index, protein flooding possibility and isotope peak distribution rationality corresponding to each target characteristic peak; screening protein flooding characteristic peaks from all target characteristic peaks; segmenting target pathogen peaks from the protein flooding characteristic peaks, and detecting respiratory tract sputum components according to the target characteristic peaks and the segmented target pathogen peaks. The present application realizes the segmentation of protein flooding characteristic peaks by analyzing the protein flooding condition in the characteristic peaks, thereby facilitating the identification of the flooded pathogen, and further improving the accuracy of respiratory tract sputum component detection.

The present invention discloses a method for identifying Mycobacterium tuberculosis in fluorescence microscopic images of sputum samples, comprising: performing multi-focal plane scanning on the sputum sample using an optical microscope to obtain a continuous fluorescence slice image sequence; processing the fluorescence slice image sequence using a three-dimensional volume reconstruction algorithm to generate three-dimensional fluorescence volume data containing spatial coordinates and fluorescence intensity information; converting the bacterial structure in the three-dimensional fluorescence volume data into a graph model, wherein nodes correspond to bacterial feature points and carry spatial coordinates and fluorescence parameters, and edges connect adjacent feature points and reflect spatial relationships; and performing feature analysis on the graph model using a graph neural network. In the present invention, high recognition accuracy is achieved, and the use of three-dimensional imaging and graph neural network technology can comprehensively obtain multi-dimensional information of the bacteria, construct a three-dimensional model and convert it into a graph model for feature analysis, effectively solving the problem of two-dimensional viewing angle limitation and greatly improving the accuracy of Mycobacterium tuberculosis identification.

The present invention discloses a sputum suction catheter for thoracic surgery nursing, which belongs to the field of sputum suction technology. It includes a sputum sorting bin, on the outer edge surface of which is installed a side sliding positioning mechanism and a switching mechanism for sliding sleeves on the side sliding positioning mechanism, wherein the side sliding positioning mechanism includes an arc sleeve frame, and a reserved opening penetrating the sputum sorting bin is provided on the side of the arc sleeve frame surface away from the interface of the sputum suction insertion tube, and through the temporary storage and sorting function of the sputum sorting bin, the sputum adsorbed by the sputum suction insertion tube can be reprocessed, and the sputum collection mechanisms of different interfaces only need to be switched to the reserved openings on the outer side of the sputum sorting bin through the switching mechanism, and the sputum collection mechanisms on different endpoints can be aligned as needed to adsorb sputum in different states, and the impurity liquid in the oral cavity or nasal cavity can be sorted and collected, solving the problem that the conventional sputum suction catheter in the prior art cannot effectively separate and collect qualified sputum specimens.

The invention relates to a method and a kit for detecting a rifampicin drug resistance gene of mycobacterium tuberculosis. The kit comprises polynucleotide primers as shown in SEQ ID NO.1-SEQ ID NO.7. The detection method comprises the following steps: step 1, collecting a sample: collecting a sputum sample of a patient as a test sample; step 2, extracting DNA: extracting DNA of a test sample by using a kit method or a lysis method; 3, preparing a reaction system, and carrying out reaction amplification, namely preparing an amplification reaction system under the guidance of the special primer for the kit by taking the sample DNA in the step 2 as a template, and carrying out specific amplification; step 4, result analysis: in the specific amplification process, due to the difference of amplification target sequences, the basic group contents of amplification products are different; the change of a single basic group can cause the change of the melting temperature of amplified target double chains; the method comprises the following steps: monitoring the change of a fluorescence signal in a temperature rise process in real time, performing data integration and image drawing on a detection result to generate a target product melting curve, and judging the difference of a gene sequence in a target product according to the difference of the melting curve.

The sputum sample cup comprises a cup body, a first cup cover, a second cup cover and a stirring rod, a first connecting piece is integrally formed at the top of the cup body, the first cup cover is movably connected with the first connecting piece, a second connecting piece is arranged in the center of the inner wall of the first cup cover, and the second cup cover is movably connected with the second connecting piece. A flow guide channel is formed in the center of the stirring rod, a first through hole is formed in the center of the bottom of the stirring rod, a connector is fixedly connected to the top of the stirring rod, a second through hole is formed in the center of the top of the connector, and the stirring rod penetrates through the middle of the first cup cover and is inserted into the cup body; according to the novel sputum suction cup, sputum can be directly sucked out of the cup body through the stirring rod, diluent can also be directly guided into the bottom of the cup body through the stirring rod, the sputum and the diluent can be prevented from making contact with workers, and the safety of the operation process is greatly improved.

The application provides a lung cancer intelligent screening model construction method and system based on sputum direct mass spectrum data. The method comprises the following steps: S1, obtaining the original mass spectrum data of the sputum sample detected by neutral desorption electrospray extraction ionization mass spectrometry, preprocessing including mass spectrum peak detection, peak alignment and intensity normalization to form an initial feature matrix; S2, extracting a core metabolic fingerprint feature set from the initial feature matrix; S3, constructing and training a heterogeneous integrated diagnosis model; S4, optimizing and verifying the hyperparameters of the heterogeneous integrated diagnosis model, and packaging the optimized heterogeneous integrated diagnosis model and the screening rules of the core metabolic fingerprint feature set into a lung cancer screening deployment module. The application realizes high-robustness, high-precision and high-explainability lung cancer non-invasive intelligent screening.

The invention relates to a physical host removal method for a respiratory tract sample, and belongs to the technical field of pathogen detection. The invention aims to combine a physical method to remove hosts and a chemical method to remove hosts, establish an efficient and broad-spectrum respiratory tract sample host removing method by optimizing physical homogenization parameters and combining the chemical method, and break through the bottleneck in the prior art. The detection method disclosed by the invention has the advantages that 1) the detection method can be suitable for sputum samples without adding treatment steps; 2) the human source removal efficiency is high, and compared with a chemical method for removing hosts widely applied at present, the host removal efficiency is high, and the number of microbial sequences is high; and 3) the operation is simple and convenient, and hosts can be removed within a short time.

The present invention discloses a sputum specimen collection robot, wherein a track is preset along the walking route of the device, a matching track wheel is arranged on the track, the wheel axle of the track wheel is fixed in the bearing of a base, a power motor for driving the wheel axle to rotate is arranged on the bottom surface of the base, a constant temperature box is fixed on the base, and at least one group of sampling mechanisms is arranged in the constant temperature box; the present invention has the advantages of high automation, medical personnel can complete the acquisition of sputum samples without contacting patients, and can pre-treat and crush the sputum samples, and can inactivate viruses at the same time, so that subsequent laboratory testing work will not cause external pollution and infection.

The invention belongs to the technical field of tuberculosis detection, and provides a microRNA combination for bimodal differentiation of tuberculosis infection, a kit and a detection system. Nine specific microRNAs and one specific internal reference microRNA are taken as markers, the microRNAs in plasma are rapidly released by adopting a direct splitting method, the relative expression quantity of the markers is synchronously detected in combination with a tailing method reverse transcription technology, a four-group primer grouping amplification technology and a fluorescent PCR technology, a comprehensive risk value is calculated through a built-in algorithm, and latent tuberculosis infection, active infection and a negative state are synchronously distinguished. According to the invention, sputum samples are not needed, the detection is rapid and efficient, the detection sensitivity and specificity of active tuberculosis infection reach 92%, the detection sensitivity and specificity of latent tuberculosis infection reach 93%, the detection specificity reaches 90%, the operation is convenient, and the application range is wide.

A system and method for analyzing a sputum sample from a subject suspected of having lung cancer comprising obtaining a plurality of cells from the sputum sample from the subject, marking the plurality of cells with i) a plurality of cell lineage specific marker compositions, ii) a cell viability composition and iii) a tetra (4-carboxyphenyl) porphyrin (TCPP) composition; analyzing with the flow cytometer the plurality of cells marked with i-iii to obtain a subpopulation selected for cell size from the plurality of cells based upon an automatically selected bead size exclusion gate; from the cell size selected subpopulation, selecting a viable singlet population of cells using an automated non-debris gate and an automated singlets gate; from the viable singlet population of cells, obtaining flow cytometer values based upon the plurality of cell lineage specific marker compositions, the viability marker and the TCPP marker; applying a trained classifier to meta data from the subject and the flow cytometric values obtained; and generating, based upon the application of the trained classifier, a classification for the sputum sample wherein the classification is selected from a plurality of classification options comprising cancer and non-cancer.

The invention discloses a sputum sample pretreatment device for microbial etiology inspection, and relates to the technical field of microbial etiology inspection. Comprising a machine shell, a bottom plate is arranged on the bottom wall of an inner cavity of the machine shell, a lifting motor unit and an ultrasonic unit are arranged on the bottom plate, a positioning plate is connected to the lifting motor unit, a proximity switch unit is arranged on the positioning plate, the proximity switch unit is electrically connected with the ultrasonic unit, a placing block is arranged on the ultrasonic unit, and the placing block is electrically connected with the ultrasonic unit. A sputum taking cup containing cavity is formed in the containing block, a clamping mechanism is arranged on the outer side of the sputum taking cup containing cavity, and the clamping mechanism is connected with the lifting motor unit through a connecting rod. The ultrasonic unit generates ultrasonic waves to quickly and effectively homogenize the phlegm reducing liquid, so that the detection efficiency of microbial etiology inspection is improved, and the pretreatment cost is reduced.

The invention relates to a sputum sample collecting device and method for tuberculosis detection, and relates to the technical field of auxiliary detection instruments, the sputum sample collecting device comprises a transparent container and a floating plugging mechanism, the interior of the transparent container is divided into a sampling bin and a waste liquid bin which are communicated through a shunting channel, and the side wall of the transparent container is provided with first, second and third scales; the third scale is flush with the lower edge of the opening of the shunting channel sampling bin; a sample inlet is formed in the top end of the transparent container; when the liquid level of the sampling bin reaches a first scale, the floating plugging mechanism opens the shunting channel, and liquid at the upper part of the sampling bin flows into the waste liquid bin; when the liquid level drops to the third scale, the floating plugging mechanism closes the channel, and the second scale is used for indicating the final sample liquid level in the sampling bin when sampling is completed. Through linkage of the floating plugging mechanism and the shunting channel, impurities are effectively shunted, only deep sputum is reserved, impurity mixing is reduced, and the specimen quality is guaranteed.

The invention discloses a sputum sample pretreatment medical examination device, and relates to the field of medical examination equipment, the sputum sample pretreatment medical examination device comprises a water tank seat and a mounting plate, the top of the water tank seat is provided with a vortex device, the top of the vortex device is provided with a mixing cup, one side of the mounting plate is provided with a rotating mechanism, and the output end of the rotating mechanism is connected with a fixing ring; a fixing mechanism in the fixing ring is used for fixing the test tube, a mounting seat is arranged at the position, located above the vortex device, of one side of the mounting plate, and a rotating ring is rotationally arranged on the inner wall of the mounting seat. A test tube filled with a sputum sample is rotated to the position above the mixing cup through the rotating mechanism and the fixing mechanism and falls into the mixing cup, meanwhile, a reagent liquid sprayed out of the spray head is mixed with the sputum sample, the mixing effect of the reagent liquid and the sputum sample is effectively improved, the interior of the test tube can be cleaned through the reagent liquid, and the cleaning efficiency is improved. The pollution of the sputum sample to the surrounding environment is effectively prevented.

The application discloses a sputum liquefaction method and a special liquefaction reagent thereof, and belongs to the technical field of biotechnology. The sputum liquefaction method comprises the following steps: mixing the sputum liquefaction reagent with a sputum sample, and then performing high-temperature treatment or high-temperature and ultrasonic combined treatment. The sputum can be completely liquefied and nucleic acid can be released from pathogens in one step, the nucleic acid of the pathogens can be protected from degradation at the same time, the operation is simple and convenient, the time consumption is short, the liquefaction efficiency of the sputum sample can be greatly improved, and the positive detection rate can be obviously improved. The sputum liquefaction reagent has simple composition and low component concentration, and the production cost can be greatly reduced.

The invention discloses a primer, a probe and a microarray chip for detecting mycobacterium tuberculosis and drug resistance of the mycobacterium tuberculosis, the screened primer and the probe can be used for detecting the drug resistance of the mycobacterium tuberculosis and isoniazide and rifampicin through PCR (Polymerase Chain Reaction) amplification and fluorescence scanning by taking sputum as a sample; the screened probe can be specifically combined with a corresponding target gene. The microarray chip prepared by utilizing the screened probe can be specifically combined with a corresponding target gene, is relatively strong and stable in hybridization signal, can take a sputum sample as a detection object, and does not need invasion or anesthesia. The invention establishes a microarray chip detection method which is good in specificity, high in sensitivity, strong in stability, time-saving and labor-saving.

The utility model discloses a sputum specimen collecting device. The sputum specimen collecting device comprises a cup body, the cup body is connected with a cleaning assembly; the cup body is connected with an anti-sputtering assembly; the cleaning assembly comprises a uniform spraying cover, a water outlet hole and a water inlet pipe; the anti-sputtering assembly comprises a collecting cover, a slope, an inlet, a protective cover, a limiting rod and an L-shaped groove. The cup body is placed in the cleaning pool, external cleaning liquid enters the water inlet pipe through the pipeline and is collected in the uniform spraying cover, and the cleaning liquid is uniformly sprayed to the cup body and the anti-sputtering assembly through the water outlet holes, so that cleaning of the cup body and the anti-sputtering assembly is achieved, germ leakage is avoided, and health of medical staff is protected. The protective cover is pressed downwards from the top of the collecting cover, the protective cover is rotated clockwise, when the limiting rod is limited in the L-shaped groove, rotation is stopped, a patient spits towards the slope, splashed sputum is blocked by the protective cover and flows to the slope and the inlet along the protective cover, and therefore virus spreading caused by sputum splashing is prevented.

This invention provides a sputum sampling device, belonging to the field of medical device technology. The sample tube has a sealing cap at the top. A first one-way exhaust valve and a first one-way intake valve are respectively installed at both ends of the negative pressure airbag. The two ends of the negative pressure tube are connected to the sample tube and the first one-way exhaust valve, respectively. The sputum collection tube includes a first tube segment and a second tube segment connected in sequence. One end of the first tube segment has a sputum collection tip, and one end of the second tube segment is connected to the inner cavity of the sample tube. The backflush assembly includes a three-way switching valve, a backflush airbag, and a backflush tube. The three-way switching valve is located at the end of the second tube segment near the first tube segment. A second one-way exhaust valve and a second one-way intake valve are respectively installed at both ends of the backflush airbag. The backflush tube is connected to the second one-way exhaust valve and the three-way switching valve. This device can collect sputum from patients and simultaneously backflush some of the sputum sample remaining in the sputum collection tube back into the sample tube, reducing sputum sample waste and ensuring the accuracy of the test results.

This invention relates to the field of biological sample preservation technology, specifically to a room-temperature preservation solution for bacterial RNA from sputum samples of tuberculosis patients. Each 100 mL of the RNA preservation solution comprises the following ingredients: 2-5 g tris(hydroxymethyl)aminomethane, 0.5-1.3 g mucin digester, 20-30 g guanidine isothiocyanate, 0.1-0.3 g hexadecylpyridine chloride, 0.5-1.2 g ethylhexene dicysteine, 20-40 mL fixative, 1-5 g stabilizer, 0.1-0.5 g inorganic salt, 0.3-0.6 g surfactant, and 0.1-0.5 g preservative. The preservation solution provided by this invention can effectively liquefy sputum samples at room temperature, meeting the requirements for long-distance sample transportation. The RNA quality of sputum samples treated with the preservation solution of this invention is suitable for various subsequent molecular detection methods, possessing significant application value and promising prospects for widespread application.

The invention discloses a sputum suction tube bag with a sputum collector, which comprises a first suction connecting tube, a second suction connecting tube and the sputum collector, the sputum collector comprises a sputum collecting and storing bag which can be contracted or pulled up, and the sputum collecting and storing bag is made of a transparent material. The sputum collecting and storing bag is in a contracted state in a standby state and is in a pulled-open state to facilitate sputum collection in a use state, one end of the first suction connecting pipe and one end of the second suction connecting pipe are both communicated with the interior of the sputum collecting and storing bag, and the other end of the second suction connecting pipe serves as a suction device connecting end. The other end of the first suction connecting tube serves as a patient suction end, and the sputum collecting and storing bag is provided with containing marks and a sputum character label plate used for marking sputum characters. According to the sputum suction device, sputum at different parts can be completely sucked clinically only through one sputum suction device, the hospitalization cost of a patient is effectively saved, and meanwhile, sputum samples can be conveniently collected and reserved at any time during clinical sputum suction operation.

A system and method for analyzing a sputum sample from a subject suspected of having lung cancer comprising obtaining a plurality of cells from the sputum sample from the subject, marking the plurality of cells with i) a plurality of cell lineage specific marker compositions, ii) a cell viability composition and iii) a tetra (4-carboxyphenyl) porphyrin (TCPP) composition; analyzing with the flow cytometer the plurality of cells marked with i-iii to obtain a subpopulation selected for cell size from the plurality of cells based upon an automatically selected bead size exclusion gate; from the cell size selected subpopulation, selecting a viable singlet population of cells using an automated non-debris gate and an automated singlets gate; from the viable singlet population of cells, obtaining flow cytometer values based upon the plurality of cell lineage specific marker compositions, the viability marker and the TCPP marker; applying a trained classifier to meta data from the subject and the flow cytometric values obtained; and generating, based upon the application of the trained classifier, a classification for the sputum sample wherein the classification is selected from a plurality of classification options comprising cancer and non-cancer.

The invention belongs to the technical field of medical instruments, and particularly relates to a sputum collection device for thoracic surgery nursing, which comprises a machine body, and a box cover is arranged at the upper end of the machine body; the sampling part comprises a suction tube, and a sealing plug and a liquid outlet are arranged in the suction tube; the sampling part comprises a sampling tube, the sampling tube is communicated with a sputum suction tube, and the sputum suction tube is provided with a contact rod and a pull rope; the regulation and control part comprises a fixed rod, a sliding block and a movable ring are arranged on the outer ring of the fixed rod, and a clamping block is arranged in an inner cavity of the movable ring; and the positioning part comprises a rotating ring, and an inner ring of the rotating ring is connected with an insertion block. According to the sputum suction device, sputum samples can be sampled in the sputum suction process, the contact rod can be controlled to be unfolded outwards during sputum collection, the situation that a suction part is directly attached to the inner wall of the respiratory tract and hurts a patient is avoided, the outward extending length of the contact rod can be controlled, and the situation that suction of sputum attached to the wall of the respiratory tract is affected due to the fact that the width of a gap is fixed is avoided.

The application provides a lung adenocarcinoma marker circ_0006949 and application thereof in preparation of a lung adenocarcinoma screening kit or a treatment monitoring kit. The circ_0006949 in a sputum sample of a lung adenocarcinoma patient is significantly higher than that in a normal person, and the application first finds that the circ_0006949 is a biomarker for early diagnosis of lung adenocarcinoma; the sputum sample is non-invasive, and the detection price is low, so that the application is suitable for popularization and application.

The utility model provides an auxiliary accessory of a disposable bronchoscope without a dosing port, and relates to the technical field of bronchoscopes, the auxiliary accessory comprises a sputum collector, the sputum collector comprises a collector bottle body and a bottle body sealing cover, and the bottle body sealing cover is provided with a negative pressure suction tube and a sputum suction tube which are communicated with the interior of the collector bottle body; a first opening of the three-way valve assembly is used for being connected with an operation channel opening of the bronchoscope, a second opening of the three-way valve assembly is connected with the end, away from the collector bottle body, of the sputum suction pipe, and a third opening of the three-way valve assembly serves as a medicine feeding opening. According to the technical scheme, in practical application, connection with the bronchoscope does not need to be disconnected during administration, and only the branch needs to be switched and conducted through the three-way valve assembly, so that the risk that a sputum sample is polluted can be effectively reduced, and operation convenience is improved for nurses.