32 results about "Sputum sample" patented technology

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The utility model discloses an integrally formed sputum collector, which belongs to the technical field of medical instruments and comprises a collector, a valve body component and a drainage pipe fitting, the front end of the valve body component is communicated with a first suction tube, the tail end of the valve body component is communicated with a second suction tube, the drainage pipe fitting is arranged between the sputum collector and the valve body component, and the first suction tube is communicated with the first suction tube. Through the cooperation between the valve body assembly and the drainage pipe fitting, the communication state between the collector and the bronchofiberscope and the communication state between the suction device and the bronchofiberscope can be adjusted and controlled at any time, it is not needed that sampling is conducted again after the suction device sucks and collects sputum, and in the working process of the bronchofiberscope, sampling is conducted again. Sputum is sampled by the collector in a negative pressure state formed by the suction treatment of the suction device, so that a sputum collection sample independently collected in the suction device can be inspected, the sputum sample does not need to be obtained from a collection part in the suction device, the operation steps are reduced, and the detection efficiency is improved. And convenience is provided for surgical operation of medical personnel.

The invention relates to the technical field of component detection, in particular to a critical patient respiratory tract sputum component rapid detection system based on mass spectrum, which can realize the following steps through mutual cooperation of a plurality of modules: obtaining a target mass spectrum corresponding to a respiratory tract sputum sample of a patient to be detected, identifying a target characteristic peak in the target mass spectrum and an isotope peak cluster corresponding to the target characteristic peak; determining pathogen conformance indexes, protein flooding possibility and isotope peak distribution rationality corresponding to each target characteristic peak; screening out protein submerging characteristic peaks from all the target characteristic peaks; and segmenting a target pathogen peak from the protein submerging characteristic peak, and carrying out respiratory tract sputum component detection according to the target characteristic peak and the segmented target pathogen peak. By analyzing the protein submerging condition in the characteristic peak, the segmentation of the protein submerging characteristic peak is realized, so that the identification of the submerged pathogen is facilitated, and the accuracy of detecting the components of the sputum in the respiratory tract is further improved.

The three-dimensional propaganda and education model comprises a model main body, an identification assembly and a supporting base, the model main body is made of a transparent material, and an unqualified sputum specimen simulation unit (such as saliva and a polluted sputum specimen) and a qualified sputum specimen simulation unit (such as white viscous sputum, yellow viscous sputum and bloody sputum) are arranged in the model main body; each unit is of an independent cavity structure, and the sputum character is visually displayed by accurately controlling the color and viscosity and simulating particles (such as squamous epithelial cells or inflammatory cells); the identification assembly is used for clearly marking the type and the qualified standard through an identification board and a fluorescent coating; the supporting base provides stable placement. Through materialized comparison, the patient can quickly distinguish the sputum type, so that the retention quality is improved from the source, the percent of pass is remarkably improved, the nursing load is reduced, and the method has the advantages of intuition, standardization, high efficiency, safety and the like.

The present application relates to the technical field of component detection, in particular to a rapid detection system for respiratory tract sputum components of severe patients based on mass spectrometry. The system can realize the following steps through the cooperation between multiple modules: obtaining a target mass spectrum corresponding to a respiratory tract sputum sample of a patient to be detected, and identifying a target characteristic peak and its corresponding isotope peak cluster in the target mass spectrum; determining a pathogen compliance index, protein flooding possibility and isotope peak distribution rationality corresponding to each target characteristic peak; screening protein flooding characteristic peaks from all target characteristic peaks; segmenting target pathogen peaks from the protein flooding characteristic peaks, and detecting respiratory tract sputum components according to the target characteristic peaks and the segmented target pathogen peaks. The present application realizes the segmentation of protein flooding characteristic peaks by analyzing the protein flooding condition in the characteristic peaks, thereby facilitating the identification of the flooded pathogen, and further improving the accuracy of respiratory tract sputum component detection.

The application provides a lung cancer intelligent screening model construction method and system based on sputum direct mass spectrum data. The method comprises the following steps: S1, obtaining the original mass spectrum data of the sputum sample detected by neutral desorption electrospray extraction ionization mass spectrometry, preprocessing including mass spectrum peak detection, peak alignment and intensity normalization to form an initial feature matrix; S2, extracting a core metabolic fingerprint feature set from the initial feature matrix; S3, constructing and training a heterogeneous integrated diagnosis model; S4, optimizing and verifying the hyperparameters of the heterogeneous integrated diagnosis model, and packaging the optimized heterogeneous integrated diagnosis model and the screening rules of the core metabolic fingerprint feature set into a lung cancer screening deployment module. The application realizes high-robustness, high-precision and high-explainability lung cancer non-invasive intelligent screening.

The invention relates to a physical host removal method for a respiratory tract sample, and belongs to the technical field of pathogen detection. The invention aims to combine a physical method to remove hosts and a chemical method to remove hosts, establish an efficient and broad-spectrum respiratory tract sample host removing method by optimizing physical homogenization parameters and combining the chemical method, and break through the bottleneck in the prior art. The detection method disclosed by the invention has the advantages that 1) the detection method can be suitable for sputum samples without adding treatment steps; 2) the human source removal efficiency is high, and compared with a chemical method for removing hosts widely applied at present, the host removal efficiency is high, and the number of microbial sequences is high; and 3) the operation is simple and convenient, and hosts can be removed within a short time.

The invention belongs to the technical field of tuberculosis detection, and provides a microRNA combination for bimodal differentiation of tuberculosis infection, a kit and a detection system. Nine specific microRNAs and one specific internal reference microRNA are taken as markers, the microRNAs in plasma are rapidly released by adopting a direct splitting method, the relative expression quantity of the markers is synchronously detected in combination with a tailing method reverse transcription technology, a four-group primer grouping amplification technology and a fluorescent PCR technology, a comprehensive risk value is calculated through a built-in algorithm, and latent tuberculosis infection, active infection and a negative state are synchronously distinguished. According to the invention, sputum samples are not needed, the detection is rapid and efficient, the detection sensitivity and specificity of active tuberculosis infection reach 92%, the detection sensitivity and specificity of latent tuberculosis infection reach 93%, the detection specificity reaches 90%, the operation is convenient, and the application range is wide.

The invention discloses a sputum sample pretreatment device for microbial etiology inspection, and relates to the technical field of microbial etiology inspection. Comprising a machine shell, a bottom plate is arranged on the bottom wall of an inner cavity of the machine shell, a lifting motor unit and an ultrasonic unit are arranged on the bottom plate, a positioning plate is connected to the lifting motor unit, a proximity switch unit is arranged on the positioning plate, the proximity switch unit is electrically connected with the ultrasonic unit, a placing block is arranged on the ultrasonic unit, and the placing block is electrically connected with the ultrasonic unit. A sputum taking cup containing cavity is formed in the containing block, a clamping mechanism is arranged on the outer side of the sputum taking cup containing cavity, and the clamping mechanism is connected with the lifting motor unit through a connecting rod. The ultrasonic unit generates ultrasonic waves to quickly and effectively homogenize the phlegm reducing liquid, so that the detection efficiency of microbial etiology inspection is improved, and the pretreatment cost is reduced.

The invention relates to a sputum sample collecting device and method for tuberculosis detection, and relates to the technical field of auxiliary detection instruments, the sputum sample collecting device comprises a transparent container and a floating plugging mechanism, the interior of the transparent container is divided into a sampling bin and a waste liquid bin which are communicated through a shunting channel, and the side wall of the transparent container is provided with first, second and third scales; the third scale is flush with the lower edge of the opening of the shunting channel sampling bin; a sample inlet is formed in the top end of the transparent container; when the liquid level of the sampling bin reaches a first scale, the floating plugging mechanism opens the shunting channel, and liquid at the upper part of the sampling bin flows into the waste liquid bin; when the liquid level drops to the third scale, the floating plugging mechanism closes the channel, and the second scale is used for indicating the final sample liquid level in the sampling bin when sampling is completed. Through linkage of the floating plugging mechanism and the shunting channel, impurities are effectively shunted, only deep sputum is reserved, impurity mixing is reduced, and the specimen quality is guaranteed.

The application discloses a sputum liquefaction method and a special liquefaction reagent thereof, and belongs to the technical field of biotechnology. The sputum liquefaction method comprises the following steps: mixing the sputum liquefaction reagent with a sputum sample, and then performing high-temperature treatment or high-temperature and ultrasonic combined treatment. The sputum can be completely liquefied and nucleic acid can be released from pathogens in one step, the nucleic acid of the pathogens can be protected from degradation at the same time, the operation is simple and convenient, the time consumption is short, the liquefaction efficiency of the sputum sample can be greatly improved, and the positive detection rate can be obviously improved. The sputum liquefaction reagent has simple composition and low component concentration, and the production cost can be greatly reduced.

This invention provides a sputum sampling device, belonging to the field of medical device technology. The sample tube has a sealing cap at the top. A first one-way exhaust valve and a first one-way intake valve are respectively installed at both ends of the negative pressure airbag. The two ends of the negative pressure tube are connected to the sample tube and the first one-way exhaust valve, respectively. The sputum collection tube includes a first tube segment and a second tube segment connected in sequence. One end of the first tube segment has a sputum collection tip, and one end of the second tube segment is connected to the inner cavity of the sample tube. The backflush assembly includes a three-way switching valve, a backflush airbag, and a backflush tube. The three-way switching valve is located at the end of the second tube segment near the first tube segment. A second one-way exhaust valve and a second one-way intake valve are respectively installed at both ends of the backflush airbag. The backflush tube is connected to the second one-way exhaust valve and the three-way switching valve. This device can collect sputum from patients and simultaneously backflush some of the sputum sample remaining in the sputum collection tube back into the sample tube, reducing sputum sample waste and ensuring the accuracy of the test results.

This invention relates to the field of biological sample preservation technology, specifically to a room-temperature preservation solution for bacterial RNA from sputum samples of tuberculosis patients. Each 100 mL of the RNA preservation solution comprises the following ingredients: 2-5 g tris(hydroxymethyl)aminomethane, 0.5-1.3 g mucin digester, 20-30 g guanidine isothiocyanate, 0.1-0.3 g hexadecylpyridine chloride, 0.5-1.2 g ethylhexene dicysteine, 20-40 mL fixative, 1-5 g stabilizer, 0.1-0.5 g inorganic salt, 0.3-0.6 g surfactant, and 0.1-0.5 g preservative. The preservation solution provided by this invention can effectively liquefy sputum samples at room temperature, meeting the requirements for long-distance sample transportation. The RNA quality of sputum samples treated with the preservation solution of this invention is suitable for various subsequent molecular detection methods, possessing significant application value and promising prospects for widespread application.

A system and method for analyzing a sputum sample from a subject suspected of having lung cancer comprising obtaining a plurality of cells from the sputum sample from the subject, marking the plurality of cells with i) a plurality of cell lineage specific marker compositions, ii) a cell viability composition and iii) a tetra (4-carboxyphenyl) porphyrin (TCPP) composition; analyzing with the flow cytometer the plurality of cells marked with i-iii to obtain a subpopulation selected for cell size from the plurality of cells based upon an automatically selected bead size exclusion gate; from the cell size selected subpopulation, selecting a viable singlet population of cells using an automated non-debris gate and an automated singlets gate; from the viable singlet population of cells, obtaining flow cytometer values based upon the plurality of cell lineage specific marker compositions, the viability marker and the TCPP marker; applying a trained classifier to meta data from the subject and the flow cytometric values obtained; and generating, based upon the application of the trained classifier, a classification for the sputum sample wherein the classification is selected from a plurality of classification options comprising cancer and non-cancer.

The invention belongs to the technical field of medical instruments, and particularly relates to a sputum collection device for thoracic surgery nursing, which comprises a machine body, and a box cover is arranged at the upper end of the machine body; the sampling part comprises a suction tube, and a sealing plug and a liquid outlet are arranged in the suction tube; the sampling part comprises a sampling tube, the sampling tube is communicated with a sputum suction tube, and the sputum suction tube is provided with a contact rod and a pull rope; the regulation and control part comprises a fixed rod, a sliding block and a movable ring are arranged on the outer ring of the fixed rod, and a clamping block is arranged in an inner cavity of the movable ring; and the positioning part comprises a rotating ring, and an inner ring of the rotating ring is connected with an insertion block. According to the sputum suction device, sputum samples can be sampled in the sputum suction process, the contact rod can be controlled to be unfolded outwards during sputum collection, the situation that a suction part is directly attached to the inner wall of the respiratory tract and hurts a patient is avoided, the outward extending length of the contact rod can be controlled, and the situation that suction of sputum attached to the wall of the respiratory tract is affected due to the fact that the width of a gap is fixed is avoided.

The application provides a lung adenocarcinoma marker circ_0006949 and application thereof in preparation of a lung adenocarcinoma screening kit or a treatment monitoring kit. The circ_0006949 in a sputum sample of a lung adenocarcinoma patient is significantly higher than that in a normal person, and the application first finds that the circ_0006949 is a biomarker for early diagnosis of lung adenocarcinoma; the sputum sample is non-invasive, and the detection price is low, so that the application is suitable for popularization and application.

The utility model provides an auxiliary accessory of a disposable bronchoscope without a dosing port, and relates to the technical field of bronchoscopes, the auxiliary accessory comprises a sputum collector, the sputum collector comprises a collector bottle body and a bottle body sealing cover, and the bottle body sealing cover is provided with a negative pressure suction tube and a sputum suction tube which are communicated with the interior of the collector bottle body; a first opening of the three-way valve assembly is used for being connected with an operation channel opening of the bronchoscope, a second opening of the three-way valve assembly is connected with the end, away from the collector bottle body, of the sputum suction pipe, and a third opening of the three-way valve assembly serves as a medicine feeding opening. According to the technical scheme, in practical application, connection with the bronchoscope does not need to be disconnected during administration, and only the branch needs to be switched and conducted through the three-way valve assembly, so that the risk that a sputum sample is polluted can be effectively reduced, and operation convenience is improved for nurses.

The invention provides an in-situ inactivation stabilization and rapid mass spectrometry detection method for a sputum sample, which comprises the following steps: mixing the sputum sample collected in a sputum collector with a stabilizer on a sample collection site, and sealing and uniformly shaking to obtain a viscous sample mixed solution; the stabilizer is an ethanol solution with the volume percent of 75-95%; the sealed sputum collector is stored and transferred at the environment temperature of 4-30 DEG C; the transferred viscous sample mixed solution is directly fed into a neutral desorption electrospray extraction ionization ion source, the viscous sample mixed solution is atomized into neutral aerosol plume, the neutral aerosol plume and the charged electrospray extraction plume are subjected to cross collision in space, and molecules in the sample are directly desorbed and ionized under normal pressure; the method comprises the following steps: acquiring a mass spectrum fingerprint spectrogram of a sputum sample through mass spectrum detection, inputting the mass spectrum fingerprint spectrogram into a database, and classifying and distinguishing the mass spectrum fingerprint spectrogram by utilizing a preset mathematical model. The method is simple in process, low in cost and easy to standardize.

The utility model discloses a medical examination equipment technical field's a novel sputum sample automatic inoculation appearance, including the box, the inner side fixed mounting of box has the baffle, the bottom fixed mounting of baffle has the motor, the top limiting of baffle rotates and is equipped with the support shaft, the top fixed mounting of support shaft has the placing disc, the surface of placing disc is around the disc center circle and is equipped with multiple sets of limit slot, the inner side of limit slot is provided with sample test tube, the bottom of support shaft is fixedly connected with the top output end of motor, the inner side bottom of box is installed respectively first digestive juice storage jar, second digestive juice storage jar and third digestive juice storage jar, through the lifting type ultrasonic wave breaking head, centrifugal rotation placing disc, vibrator, multistage digestive liquid jar, six axle mechanical arm and petri dish of setting, realize to sputum sample automatic processing - centrifugal - inoculation integrated design, reduce the manual intervention link, improve the use effect of device.

1. Name of the product in this design: Sputum Sample Collection Box. 2. Purpose of this design: This design is used for storing and transporting sputum samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key features: the front view.

The invention relates to a medical sample collection instrument, and discloses a mycobacterium tuberculosis nucleic acid detection sputum sample collection device which comprises a collection pipe, a filter membrane is arranged in the collection pipe, a sealing mechanism is installed at the top of the collection pipe, an extraction mechanism is slidably connected to the top of the sealing mechanism, and the sealing mechanism comprises an opening and closing assembly. The opening and closing assembly comprises a cam disc and a pin shaft, an arc-shaped groove position is formed in the surface of the cam disc, the pin shaft is slidably connected to the inner wall of the inner arc-shaped groove position, a baffle is fixedly connected to the bottom of the pin shaft, a chassis is slidably connected to the bottom of the baffle, a sliding rail is arranged on the surface of the top of the chassis, and the baffle moves along the sliding rail. By means of the arc-shaped groove position structure on the surface of the cam disc, the traction pin shaft slides synchronously, then the baffle is driven to move front and back along the sliding rail at the bottom of the chassis, operation steps are greatly simplified, loading and taking out of samples can be completed more efficiently, and convenience of clinical operation is remarkably improved.

The invention relates to the technical field of in-vitro diagnosis, in particular to a kit for extracting and purifying fungal nucleic acid in a sputum sample and a use method. The nucleic acid extraction and purification kit provided by the invention comprises a sputum treatment solution, a pre-lysis solution, a nucleic acid releasing agent, magnetic beads, a lysis solution, a rinsing solution I, a rinsing solution II and an eluent. The invention provides a method capable of quickly liquefying sputum and efficiently, simply and conveniently extracting fungal nucleic acid from the sputum, nucleic acid in fungi is released by providing a buffer environment and using a nucleic acid releasing agent to quickly break walls, and the nucleic acid is treated and purified step by step, so that amplification inhibitor residues can be removed, and the yield of the fungal nucleic acid is increased. The yield and the purity of nucleic acid can be greatly improved. The nucleic acid extraction and purification kit can be matched with an automatic nucleic acid extractor for nucleic acid extraction and purification, pollution is reduced, and the kit has important significance in nucleic acid extraction of respiratory tract infection samples.

The utility model relates to the technical field of sputum sample collection, and discloses a tuberculosis patient sputum sample collecting device which comprises a collecting container, a sealing cover is in threaded connection with the top end of the collecting container, a rubber ring is arranged at the joint of the bottom end of the sealing cover and the collecting container, a sealing mechanism is arranged in the middle of the sealing cover, and two communicating pipe ports are symmetrically and fixedly connected with the top end of the sealing cover. And the two communicating pipe ports are communicated with the collecting container. The sputum collecting device solves the technical problems that the sealing effect of a traditional sputum collecting device does not meet the actual use requirement, the detection result is finally affected, and the treatment effect of a patient is poor. By means of the closed mechanism, during sputum taking operation and after sputum taking operation, air tightness in the collecting container can be effectively guaranteed, the probability of interaction with the outside world is reduced, sampled sputum is prevented from being polluted, effectiveness of sample data is effectively improved, and therefore treatment strategies can be adjusted in a targeted mode in cooperation with detection results; the treatment effect is improved and the pain of patients is reduced.

The utility model relates to the technical field of medical instruments, and discloses a sputum sample collecting device which comprises a sample bottle and a bottle plug, the bottle plug is connected with a negative pressure pipe and an extraction pipe, the extraction pipe is connected with a saliva separator, and the negative pressure pipe is provided with a first branch pipe communicated with the saliva separator; the saliva separator comprises a connecting sleeve, an annular cavity is formed in a sleeve body of the connecting sleeve, the annular cavity and the connecting sleeve are coaxially arranged, and a plurality of filtering holes communicated with the annular cavity are further formed in the inner wall of the connecting sleeve; a second branch pipe communicated with the first branch pipe is arranged on the outer wall of the connecting sleeve, and the second branch pipe is communicated with the annular cavity. Saliva in the extracted sputum can be removed, the content of the saliva in the sputum is reduced, the difficulty of sample treatment is reduced, and the accuracy of sputum sample detection is improved.

The utility model relates to a sputum sample collecting and smearing device. The sputum sample collecting and smearing device comprises a liquid storage tube, a sample suction tube, a smearing head, a push rod head, a return spring, a push rod and a first plug. The first chock plug is ingeniously arranged in the middle of the push rod, the sample suction pipe is arranged on the side wall of the liquid storage pipe, pretreatment liquid is preset at the bottom of the liquid storage pipe, and the sealing film is arranged at the lower end of the liquid storage pipe, so that suction of a sputum sample can be completed through cooperation of the first chock plug and the liquid suction pipe; the sputum suction device has the advantages that the sputum suction device is simple in structure and convenient to use, a sucked sputum sample can be pretreated through pretreatment liquid, the viscosity of the sputum sample is reduced, smearing of the diluted sputum sample is facilitated, the sealing film can be punctured through the front end of the push rod, the containing cavity is communicated with the smearing head, efficient smearing of the diluted sputum sample on the glass slide is facilitated, and three purposes are achieved through one action.

The invention relates to the technical field of biological sample preserving fluid, in particular to normal-temperature preserving fluid for bacterial RNA (Ribonucleic Acid) of a sputum sample of a tuberculosis patient. Every 100 mL of the RNA preserving fluid is prepared from the following raw materials: 2 to 5 g of tris (hydroxymethyl) aminomethane, 0.5 to 1.3 g of a mucus digesting agent, 20 to 30 g of guanidine isothiocyanate, 0.1 to 0.3 g of cetylpyridinium chloride, 0.5 to 1.2 g of ethylidene dicysteine, 20 to 40 mL of a fixing agent, 1 to 5 g of a stabilizing agent, 0.1 to 0.5 g of inorganic salt, 0.3 to 0.6 g of a surfactant and 0.1 to 0.5 g of a preservative. The preserving fluid provided by the invention can effectively liquefy a sputum sample under a normal temperature condition, and meets the long-distance transportation requirement of the sample. The RNA quality of a sputum sample treated by the preserving fluid is suitable for a plurality of subsequent molecule detection methods, and the preserving fluid has important application value and popularization prospect.

The application provides a kind of whole automatic pretreatment and smear staining system of tuberculosis sputum specimen, and relates to the technical field of specimen processing.The whole automatic pretreatment and smear staining system of tuberculosis sputum specimen includes specimen receiving and bar code identification module, specimen quality automatic evaluation module, automatic liquefaction inactivation module, automatic centrifugal bacterium aggregation module, automatic smear module, automatic drop dyeing and staining module, automatic drying module, waste liquid and waste automatic treatment module and central control unit arranged in sequence:the specimen quality automatic evaluation module adopts image recognition technology combined with viscosity detection technology to grade evaluate the quality of sputum specimen;the automatic centrifugal bacterium aggregation module adopts gradient centrifugation technology.By realizing whole process closed automation, eliminating occupational exposure risk;Gradient centrifugation improves the detection rate;Standardized operation improves the consistency of results, automatic quality control, greatly improves the detection efficiency.

The invention relates to a sputum sample pretreatment solution and a preparation method thereof. A pretreatment reagent is prepared from the following components: a Tris buffer solution with the concentration of 4 to 8 mM, guanidine isothiocyanate with the concentration of 6 to 8 M, EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 2 M, lithium chloride with the concentration of 0.35 to 0.45 M, polyethylene glycol (PEG) with the concentration of 8 to 12 percent, and a proper freeze-drying reagent added into the components. The pretreatment reagent has the advantages of being short in liquefaction time, good in liquefaction effect and suitable for being used in a POCT card box, and can be suitable for detection of various pathogens.

Systems for sensitive and accurate detection of sarbecoviruses in samples from human sputum have been established. The systems can rapidly and effectively diagnose patients having suspected coronavirus infections. The systems employ Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) to identify and quantify nucleic acids specific to sarbecoviruses within samples from human patients. The systems identify viral RNAs specific for the N and ORF1 genes using a two-step real-time PCR and the SYBR Green detection method. Compositions and methods for reproducibly detecting and quantifying concentrations of sarbecoviruses with a dynamic range of at least seven orders of magnitude (2×10−4-2000 TCID50 / reaction) are described. The compositions and methods enable rapid early diagnosis of patients, as well as development of databases of patient viral titres and infectious dosages.