3 results about "Bacterial RNA" patented technology

Compounds useful as antibacterial agents, pharmaceutical compositions comprising the compounds, and methods of using the same. The compounds are believed to target bacterial RNA polymerase and NusG.

This invention relates to the field of biological sample preservation technology, specifically to a room-temperature preservation solution for bacterial RNA from sputum samples of tuberculosis patients. Each 100 mL of the RNA preservation solution comprises the following ingredients: 2-5 g tris(hydroxymethyl)aminomethane, 0.5-1.3 g mucin digester, 20-30 g guanidine isothiocyanate, 0.1-0.3 g hexadecylpyridine chloride, 0.5-1.2 g ethylhexene dicysteine, 20-40 mL fixative, 1-5 g stabilizer, 0.1-0.5 g inorganic salt, 0.3-0.6 g surfactant, and 0.1-0.5 g preservative. The preservation solution provided by this invention can effectively liquefy sputum samples at room temperature, meeting the requirements for long-distance sample transportation. The RNA quality of sputum samples treated with the preservation solution of this invention is suitable for various subsequent molecular detection methods, possessing significant application value and promising prospects for widespread application.

The invention relates to the technical field of biological sample preserving fluid, in particular to normal-temperature preserving fluid for bacterial RNA (Ribonucleic Acid) of a sputum sample of a tuberculosis patient. Every 100 mL of the RNA preserving fluid is prepared from the following raw materials: 2 to 5 g of tris (hydroxymethyl) aminomethane, 0.5 to 1.3 g of a mucus digesting agent, 20 to 30 g of guanidine isothiocyanate, 0.1 to 0.3 g of cetylpyridinium chloride, 0.5 to 1.2 g of ethylidene dicysteine, 20 to 40 mL of a fixing agent, 1 to 5 g of a stabilizing agent, 0.1 to 0.5 g of inorganic salt, 0.3 to 0.6 g of a surfactant and 0.1 to 0.5 g of a preservative. The preserving fluid provided by the invention can effectively liquefy a sputum sample under a normal temperature condition, and meets the long-distance transportation requirement of the sample. The RNA quality of a sputum sample treated by the preserving fluid is suitable for a plurality of subsequent molecule detection methods, and the preserving fluid has important application value and popularization prospect.