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Application of Hfq protein of staphylococcus aureus in preventing and treating staphylococcus aureus infection

A Staphylococcus aureus and protein technology, applied in the field of RNA chaperone molecules, can solve the problems affecting the translation of SigB protein and the transcription of crtMN

Inactive Publication Date: 2010-05-26
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Sequence analysis results showed that ssrA 138-154 This segment is complementary to the SD sequence of sigB, while gel retardation experiments confirmed that ssrA 138-154 and the 5`UTR of sigB, while ssrA 132-147 There is no such effect, indicating that ssrA may affect the translation of SigB protein, thereby affecting the transcription of crtMN

Method used

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  • Application of Hfq protein of staphylococcus aureus in preventing and treating staphylococcus aureus infection
  • Application of Hfq protein of staphylococcus aureus in preventing and treating staphylococcus aureus infection
  • Application of Hfq protein of staphylococcus aureus in preventing and treating staphylococcus aureus infection

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Embodiment

[0035] 1. Construction of Staphylococcus aureus mutant strains

[0036] Primers were designed according to the 500-800bp sequences of the upstream and downstream of the to-be-mutated gene (see Table 1), and then the two sequences were amplified by PCR; the two sequences were amplified into 1,000bp sequences by overlapping PCR and Introduced restriction site Kpn I. The Kpn I digested Kana gene was inserted into the above sequence, and then this sequence was ligated into the shuttle vector pAUL-A through EcoR I and Sal I to generate plasmid pAUL-A-Δ. S. aureus RN4220 was electrotransformed with pAUL-A-Δ, cultured at 30°C first, then cultured at 42°C, and screened for kana resistance to obtain RN4220-ΔRN4220 with the same genome. Using phage 11 Lyse the ΔRN4220 culture, add the lysed supernatant to the S.aureus 8325-4 culture, incubate at 37°C for 1 hour, and then further screen at 42°C and kana resistance to obtain double crossover of the S.aureus 8325-4 genome recombinant b...

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Abstract

The invention belongs to the field of biotechnology, relating to the application of Hfq protein of staphylococcus aureus. Used as chaperone molecule of tmRNA-ssrA in staphylococcus aureus, the Hfq protein Hfq protein participates in the synthesis of adjusting and controlling staphylococcus aureus superficial pigment, thereby affecting the capability of graft-versus-host immunity of the staphylococcus aureus. Thus, the Hfq protein has good application prospect in the field of preventing and treating the staphylococcus aureus infection.

Description

Field of invention: [0001] The invention belongs to the field of biotechnology, and relates to the application of Staphylococcus aureus Hfq protein, specifically, the invention relates to Hfq protein as an RNA chaperone molecule, which participates in regulating the synthesis of surface pigments of Staphylococcus aureus, thereby affecting the ability of Staphylococcus aureus to resist host immunity . Therefore, the Hfq protein has a good application prospect in the prevention and treatment of Staphylococcus aureus infection. Background technique: [0002] Staphylococcus aureus (Staphylococcus aureus) is an important human pathogen, which can cause pneumonia, endocarditis, burn and war wound infection, sepsis, toxic shock and other serious infections. What's more serious is that the abuse of antibiotics has led to the increasing drug resistance of S. aureus, such as methicillin-resistant S. Glycopeptide-resistant Staphylococcus aureus (GISA) of peptide antibiotics, and vanc...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K39/085A61K48/00A61P31/04C12N15/31C12R1/445
Inventor 杨光刘玉吴娜董洁高亚萍邵宁生
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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