2 results about "Alpha-toxin" patented technology

The invention relates to clostridium perfringens in excrement as a molecular marker for grading and judging fatty liver diseases related to metabolic dysfunction and application of the clostridium perfringens, the clostridium perfringens contains alpha toxin CPA according to the types of main toxins generated by the clostridium perfringens, the coding gene of the alpha toxin CPA is plc gene, and the content of the alpha toxin CPA is determined by quantitatively detecting the plc gene. The molecular level detection of the clostridium perfringens in the excrement sample is realized; besides, the clostridium perfringens further contain nirA genes related to nitrogen metabolism, the nirA genes participate in the metabolic process related to microbial ammonia production, the functional state of the intestinal ammonia-producing microorganisms is reflected by detecting the expression level of the nirA genes, stable acquisition of the molecular characteristics of the intestinal microorganisms related to the MASLD is achieved, and the application prospect is broad. A molecular basis is provided for analyzing the correlation between the abnormal ammonia metabolism and the occurrence and development of the MASLD.

This invention belongs to the field of pathogen and toxin detection technology, specifically relating to a polyclonal antibody colloidal gold test strip for detecting Clostridium perfringens α toxin and its application. The polyclonal antibody colloidal gold test strip includes a PVC base plate, on which a sample pad, a gold-labeled pad, a chromatography membrane, and an absorbent pad are sequentially overlapped. The gold-labeled pad is coated with colloidal gold particles labeled with polyclonal antibodies against Clostridium perfringens α toxin. A detection line and a control line are sequentially arranged on the chromatography membrane. The detection line is coated with polyclonal antibodies against Clostridium perfringens α toxin, and the control line is coated with goat anti-rabbit IgG (H+L) antibody. The amino acid sequence of the recombinant protein of Clostridium perfringens α toxin is shown in SEQ ID NO.3. The polyclonal antibody colloidal gold test strip provided by this invention can be used for the detection of Clostridium perfringens α toxin, with a detection limit of 31.25 μg / mL.