12results about How to "Reduce Biosecurity Risks" patented technology

The utility model discloses a mixed specimen cup for sample collection, which belongs to the field of medical detection, and comprises a cup body and a cup cover matched with the open end of the cup body, the cup cover is provided with a liquid storage cabin extending into the cup body, the lower end of the liquid storage cabin is open, the open part is sealed by a sealing film, a puncture part is arranged in the liquid storage cabin, and the lower end of the liquid storage cabin is connected with the puncture part. A sharp part is arranged at the lower end of the puncture part, the upper end of the puncture part is connected with a pressing part, and the pressing part is arranged on the cup cover. The utility model aims to provide a mixed specimen cup for sample collection, which can improve the detection efficiency.

The invention belongs to the technical field of medical instruments, and particularly discloses a urine cup with a humidity indication color-changing window, which comprises a cup body, the cross section of the cup body is of an inverted L-shaped structure, a threaded ring is arranged in the middle of the upper end of the cup body, a cup cover is arranged on the outer wall of the threaded ring in a threaded manner, and a sealing gasket is arranged at the upper end of the inner wall of the cup cover. The lower end of the sealing gasket is connected with the upper end of the threaded ring, a color developing mechanism is arranged on the upper portion of the outer wall of the cup body and comprises a clamping ring, a clamping groove is formed in the position, corresponding to the clamping ring, of the cup body, the clamping ring is clamped into the clamping groove, a first color developing ring is arranged on the outer wall of the clamping ring, and a locking mechanism is slidably arranged on the outer wall of the first color developing ring. The comprehensive protection of real-time visualization of the pollution state, rapid isolation of the pollution area and transfer unsealing prevention is achieved, and the biological safety and reliability of the whole-process operation of the urine sample are remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to preparation and application of a swine A type FMDV VP1 antigen and single factor positive serum. The sequence of the expression gene of the swine A type FMDV VP1 antigen is as shown in SEQ ID NO. 1. Through the integral technical scheme of combining sequence-driven conservative site screening, expression and immunological verification, the key problems of antigen mismatch, difficult recombinant protein expression, poor diagnostic reagent universality and the like in the prior art are solved; and the effectiveness and generalizability of the vaccine, the diagnostic reagent and the monitoring means in practical application are obviously enhanced.

The application discloses a portable laboratory cold storage box with good protection, and belongs to the field of laboratory cold storage boxes. The portable laboratory cold storage box with good protection comprises a box body, a box cover hinged to the box body and locked to the box body through a lock buckle, a handle hinged to the position close to the top end of the side surface of the box body, and backpack straps fixed symmetrically on the side of the box body close to the hinged end of the box cover. An isolation frame is arranged in the box body, and the isolation frame divides the box body into three refrigeration cavities for placing ice blocks or ice bags, namely a bottom refrigeration cavity located at the bottom of the isolation frame. The portable laboratory cold storage box with good protection replaces the built-in refrigeration structure with an external cold source, significantly reduces the weight of the equipment, and cooperates with the combined design of the handle and the backpack straps to adapt to various scenes such as single-person operation, short-distance carrying or long-time carrying, and solves the problem that the traditional cold storage box is heavy and difficult to carry.

The invention discloses a totally-enclosed avian influenza H5, H7, H9 and universal virus quadruple detection kit. The kit comprises primers and probes of a universal avian influenza virus M gene, an H5 subtype avian influenza virus HA gene, an H7 subtype avian influenza virus HA gene, an H9 subtype avian influenza virus HA gene and a reference gene LconR. According to the invention, the ligand-dependent nuclear receptor corepressor protein is introduced as a reference gene, and the gene widely exists in mammals and birds and is very suitable for detection of multi-species infection epidemic diseases such as avian influenza. The internal reference can participate in nucleic acid extraction and amplification processes, strictly controls the quality of each detection step, fundamentally avoids false negative results, and significantly improves the reliability of detection results. The internal reference also expands the species application range of the detection method, so that avian influenza detection is not limited to poultry any more, and the method can be widely applied to all mammals possibly infected with avian influenza.

An integrated microbial reagent plate of a microfluidic channel integrally comprises seven parts, namely a self-sealing sample inlet (1), a tree-shaped fractal flow channel plate (2), reaction holes (3), an air bag compensation cavity (4), sponge (5), a pre-cutting groove reagent plate covering film (6) and a plate placing frame (7), and the standard inclination plate placing frame (7) is matched with the integrated microbial reagent plate for use. The integrated microbial reagent plate of the microfluidic channel is simple in structure and convenient to operate, the cost is greatly reduced, and the pollution risk can be avoided.

The application discloses a preparation method of a drug sustained-release hydrogel support and a three-dimensional osteogenic induction application of hiPSCs. Firstly, a CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere of a core-shell structure is prepared, then the freeze-dried GelMA powder is dissolved in a PBS solution containing a photoinitiator LAP, the CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere is added after stirring and cooling, and a drug-loaded hydrogel precursor is obtained; the precursor is injected into a mold and irradiated by a specific wavelength light source to initiate free radical polymerization of a methacryl group into glue, and a drug-loaded GelMA porous hydrogel support is obtained after demolding, freezing and drying; the drug-loaded GelMA porous support is covalently grafted with VN polypeptide through EDC / NHS chemical coupling to obtain a VN-GelMA drug-loaded porous hydrogel support. The application simulates the signal time sequence regulation law in the embryonic bone formation process and significantly improves the osteogenic differentiation efficiency and mineralization quality by constructing a biomimetic three-dimensional osteogenic microenvironment. The VN polypeptide enhances the specific recognition of stem cells and significantly improves the three-dimensional cell compatibility. The application can reduce the dependence on exogenous induction factors and is safe, low in cost and stable in induction.

The utility model relates to a kind of storage tank and its automatic spading device.The automatic spading device includes: push plate assembly, it includes fixed part and push plate, at least two push plates are spaced apart along the length direction of the fixed part;Drive assembly, it includes guide rail, sliding member and driving part, sliding member is slidably connected with the guide rail, and is connected with the fixed part;The driving part is drivingly connected with the sliding member, to drive the sliding member moves along the guide rail.The utility model moves by cylinder drive push plate assembly, replaces artificial spading, improves loading efficiency and reduces manpower cost and labor intensity;At the same time, by setting multiple push plates, it is realized to increase the pushing area of push plate assembly when slider moves the same distance, to increase the single pushing capacity and pushing distance of push plate assembly, to further facilitate shorten loading time and improve tank fullness coefficient.In addition, automated operation reduces artificial contact, reduces biological safety risk, reduces additional disinfection demand brought by artificial operation, reduces operating cost.

The application discloses a light-weight large-flux sterilization transfer device and method for experimental animal facilities, and belongs to the technical field of disinfection and sterilization transfer, which comprises a sterilization cabin, a loading cabin and an unloading cabin, and the loading cabin and the unloading cabin are arranged on the two sides of the sterilization cabin respectively; the sterilization cabin comprises a plastic film and a supporting frame, and the inside of the supporting frame is provided with a circulating pipeline. The application has the beneficial effects that: the supporting frame and the airtight plastic film are adopted to construct the sterilization cabin, a large flexible light-weight airtight space is formed, the device is light and easy to assemble, the vaporized hydrogen peroxide pipe is injected into the heat-insulating circulating pipeline of the supporting frame, the heat loss of the vaporized hydrogen peroxide pipe can be avoided, the vaporized hydrogen peroxide can uniformly act on the surface of the object at a stable concentration through the setting of the air inlet hole and the air return hole in the sterilization cabin, the air exchange efficiency is improved, the sterilization effect is improved, and then the vaporized hydrogen peroxide which is not fully reacted can be returned through the circulating pipeline, so that the rapid circulation is realized.

The invention discloses a microcapsule and a preparation method thereof, and self-repairing polyurethane and a preparation method and application thereof, relates to the field of polyurethane materials, and solves the problems that the self-repairing process of the self-repairing polyurethane in the prior art depends on non-physiological stimulation conditions, the cytotoxicity is high, the biosafety is insufficient, and the application requirements in the field of biomedical instruments are difficult to meet. The invention discloses a preparation method of self-repairing polyurethane, which comprises the following steps: blending a prepolymer I, a prepolymer II and a solvent, adding an Arabic gum aqueous solution and a chain extender, and adding triethanolamine to obtain a suspension containing microcapsules; and washing, carrying out solid-liquid separation, and freeze-drying to obtain the microcapsule. Adding the microcapsules into the polyurethane dioxane solution, uniformly stirring, pouring into a mold, and heating or freeze-drying in vacuum to obtain the self-repairing polyurethane. The microcapsule provided by the invention has excellent biocompatibility, solvent resistance and mechanical properties, the self-repairing condition of the self-repairing polyurethane is mild, and the application requirements in the field of biomedical instruments are met.

The invention discloses a polydopamine (PDA)-coated polylactic acid-glycolic acid (PLGA) targeted sustained release microsphere (Hep-coated PLGA-PDA MPs) loaded with heparin sodium, which is characterized in that the polylactic acid-glycolic acid microsphere (Hep-coated PLGA-PDA MPs) loaded with the drug heparin sodium is an inner core microsphere, and the outer part of the microsphere is also coated with a PDA layer. The invention also discloses a preparation method of the Hep-coated PLGA MPs, the microspheres have no burst release phenomenon within 2 hours of drug release, the release proportion of heparin sodium is 45.7-57.2% in 24 hours, and the microspheres have the characteristic of slow release. The microsphere disclosed by the invention can be used for developing an intravenous injection sustained-release agent for targeted therapy of thrombus.

A kind of secondary polishing type rodent breeding cage box, it is related to barrier environment experimental animal technical field, including cage box, the inside of the cage box is equipped with inner cavity, secondary polishing box main body is installed in the inner cavity, and secondary polishing box main body is vertically inserted with cage box and is limited, the upper end surface of secondary polishing box main body is equipped with integrally formed flange, for separating secondary polishing box main body with cage box, one side of the cage box is provided with clamping mechanism, for reinforcing installation to secondary polishing box main body, the present scheme improves animal daily feeding efficiency, while it can minimize staff operation and working hours input, avoid the problem of secondary pollution in cage box cleaning and disinfection process.