27results about How to "Reduce Biosecurity Risks" patented technology

The invention discloses a nano-carrier for gene editing of aquatic animals. The nano-carrier comprises a) TNP nano-particles and b) Cas9 mRNA and sgRNA. The invention also discloses an application of the TNP nano-particle as an mRNA delivery carrier and a gene editing method based on the nano-carrier, according to the method, the TNP nano-particle with good biocompatibility is used for packaging Cas9 mRNA and sgRNA, and an efficient and low-damage gene editing delivery mode is realized. The nano-carrier is delivered into an embryo of an aquatic animal in a non-invasive manner, so that the transfection efficiency and the specificity of gene editing are optimized. In addition, the method can improve the gene editing efficiency and precision and enhance the embryo survival rate, is simple, convenient and safe to operate and wide in applicability, can support customized gene editing, and is beneficial to promoting the application of gene editing in aquatic product germplasm genetic improvement.

The invention relates to a PPR antibacterial pipeline and a preparation method thereof, and belongs to the technical field of pipeline processing. The sea-buckthorn extract is used as an auxiliary medium, green synthesis of the silver nanoparticles can be promoted, use of traditional toxic reducing agents and stabilizing agents is avoided, natural active ingredients contained in the sea-buckthorn extract and the silver nanoparticles generate a synergistic effect, the antibacterial efficiency is remarkably enhanced, and the dosage and biotoxicity of the silver nanoparticles are reduced. Meanwhile, the nano silicon dioxide is added, so that the coating can be kept stable in a high-temperature environment. The carboxymethyl chitosan endows the pipeline with excellent antibacterial performance, and forms a synergistic effect with the silver nanoparticles, so that the antibacterial ability of the pipeline is further enhanced. Through the modification of the silane coupling agent KH560, the interface bonding force between the nano silicon dioxide and carboxymethyl chitosan and the interface bonding force between the nano silicon dioxide and the main component PPR of the pipeline are enhanced, so that the reinforcing agent can be more uniformly and stably dispersed in a PPR matrix, and the overall performance of the pipeline is improved.

The invention discloses a virus-like plasmid and application thereof. The virus-like plasmid is characterized in that mIL-21 and 4-1BBL coding genes are inserted into a lentivirus plasmid; the amino acid sequences of the coding genes of the mIL-21 and the 4-1BBL are respectively as shown in SEQ ID NO. 1 and SEQ ID NO. 2. Virus-like particles (VLPs) prepared from the virus-like plasmids are added into an NK cell culture solution, and the VLPs can synergistically stimulate the NK cells, promote the rapid growth of the NK cells (the amplification multiple reaches 1850 times after 18 days of culture), and can improve the purity (about 92%) and the killing rate (when the efficiency-target ratio is 2: 1, up to 80%) of the NK cells.

The invention discloses a method for optimizing and improving the productivity of chromium-contaminated soil by using irrigation and fertilization period and dosage combination, and the steps are: selecting a crop variety of silage corn, sowing according to the dense planting sowing amount, randomly sowing and synchronously applying compound fertilizer and humic acid fertilizer as base fertilizer, irrigating water during the sowing period; irrigating again during the jointing period or the large trumpet period of the crop, and simultaneously flushing and applying humic acid fertilizer for combined regulation; irrigating again during the heading and filling period of the crop. The method of the present invention applies humic acid fertilizer during the sowing period, jointing period or the large trumpet period, uses river water for irrigation during the sowing period, jointing period or the large trumpet period, and uses well water for irrigation during the heading and filling period to promote a significant increase in corn biomass, reduce the chromium content of the crop, and reduce the residual amount of total chromium and effective chromium in the root layer soil at the same time, and uses well water for irrigation during the heading and filling period to effectively block the transportation of effective chromium in the soil to the crop plant body, thereby achieving the improvement of chromium-contaminated farmland soil productivity and green restoration of soil chromium pollution.

The present invention discloses a method for detecting porcine Japanese encephalitis virus based on CRISPR-Cas13d system, and belongs to the field of molecular biological diagnosis technology. The method of the present invention can successfully detect clinical positive JEV samples, and can perform on-site visual detection of JEV in combination with RNA fluorescent reporter molecules, and only uses a blue light transmission instrument for irradiation, and observes whether the amplified product of the reaction system presents fluorescence to determine the test result, or uses a qPCR instrument to perform quantitative detection on it, and the result can be determined within 1h. The primers and crRNA of the present invention are highly specific, highly sensitive, and simple to operate, fast to detect, and visualized results, so that the method for detecting JEV based on RAA and CRISPR-Cas13d technology established by the present invention can be used for on-site visual detection of JEV, so as to improve the level of JEV detection in the pig farming industry and reduce the biosafety risk of farms. Provide technical means.

The utility model discloses a mixed specimen cup for sample collection, which belongs to the field of medical detection, and comprises a cup body and a cup cover matched with the open end of the cup body, the cup cover is provided with a liquid storage cabin extending into the cup body, the lower end of the liquid storage cabin is open, the open part is sealed by a sealing film, a puncture part is arranged in the liquid storage cabin, and the lower end of the liquid storage cabin is connected with the puncture part. A sharp part is arranged at the lower end of the puncture part, the upper end of the puncture part is connected with a pressing part, and the pressing part is arranged on the cup cover. The utility model aims to provide a mixed specimen cup for sample collection, which can improve the detection efficiency.

The invention relates to the technical field of handheld non-contact long-distance seed selection marking, in particular to a handheld non-contact long-distance seed selection marking device and a using method thereof.The handheld non-contact long-distance seed selection marking device comprises a grip, a ventilation switch, a multi-stage nozzle and a connecting flexible joint, the ventilation switch is installed at the upper end of the grip and connected with a gun body, and the multi-stage nozzle is connected with the gun body; the side, away from the grip, of the gun body is connected with a multi-stage nozzle through a connecting flexible joint, a positioning bent plate is installed at the upper end of the multi-stage nozzle, the other end of the positioning bent plate is fixedly connected with an auxiliary positioning unit, a control bent rod is installed on the other side of the auxiliary positioning unit, the other end of the control bent rod is fixedly connected with a half gear, and the half gear is installed in an adjusting unit. According to the pig marking device, in the process of remotely marking a pig, the multi-stage nozzles can be aligned to the center of the pig, the pig moves at the moment of marking, paint can be sprayed to the pig, and the pig marking accuracy and the marking success rate can be further improved.

The present invention relates to the technical field of medical intelligent robots, and specifically discloses a specimen receiving method, which includes: after the specimen delivery application is reviewed and approved by the specimen receiving unit server, receiving the specimen receiving instruction from the specimen receiving unit server; running to a preset position to wait for specimen reception according to the specimen receiving instruction; starting the specimen receiving program; and after the specimen receiving program is completed, sending specimen receiving completion information to the specimen receiving unit server. The present invention also discloses a specimen receiving device, a specimen receiving robot, and a specimen receiving system. The specimen receiving method provided by the present invention can complete the sample delivery without direct contact between the sample delivery personnel and the transport personnel and the specimen during the sample delivery and transportation process. This specimen receiving method not only has high transportation efficiency, but also can effectively reduce biosafety risks.

The invention discloses a construction method and application of a novel coronavirus animal infection model, and relates to the fields of molecular biology technology and animal gene engineering, the virus replicon prepared by the invention can complete multiple rounds of replication only in specific cells stably expressing novel coronavirus SARS-CoV-2N protein, but cannot be replicated in other cells, and the virus replicon can be used as a novel coronavirus animal infection model. The biological safety risk is greatly reduced, and the system can be operated in a BSL-2 laboratory. Besides, the invention successfully constructs a safe novel coronavirus SARS-CoV-2 animal infection model capable of being used in a BSL-2 laboratory by utilizing an SARS-CoV-2 trans complementary replication subsystem without an N gene and a mouse model for expressing SARS-CoV-2N protein in infected tissue specificity or specific time. The method can be applied to screening, research and development of novel coronavirus resisting drugs.

The invention belongs to the technical field of medical instruments, and particularly discloses a urine cup with a humidity indication color-changing window, which comprises a cup body, the cross section of the cup body is of an inverted L-shaped structure, a threaded ring is arranged in the middle of the upper end of the cup body, a cup cover is arranged on the outer wall of the threaded ring in a threaded manner, and a sealing gasket is arranged at the upper end of the inner wall of the cup cover. The lower end of the sealing gasket is connected with the upper end of the threaded ring, a color developing mechanism is arranged on the upper portion of the outer wall of the cup body and comprises a clamping ring, a clamping groove is formed in the position, corresponding to the clamping ring, of the cup body, the clamping ring is clamped into the clamping groove, a first color developing ring is arranged on the outer wall of the clamping ring, and a locking mechanism is slidably arranged on the outer wall of the first color developing ring. The comprehensive protection of real-time visualization of the pollution state, rapid isolation of the pollution area and transfer unsealing prevention is achieved, and the biological safety and reliability of the whole-process operation of the urine sample are remarkably improved.

The invention relates to the technical field of biological medicine detection, and discloses a tetanus antibody titer determination method, which comprises: 1, diluting a standard substance; 2, diluting a sample to be detected; step 3, adding a sample and incubating, adding the R1 reagent and the standard substance / sample, incubating, and measuring an absorbance value A1; 4, after incubation is completed, a reagent R2 is added for incubation, and the absorbance value A2 is measured; and 5, calculating an absorbance difference value A = A2-A1, sorting the absorbance value of the standard substance, fitting an absorbance-titer function by using statistical software, and calculating the titer content of the tetanus antibody according to the absorbance value of the sample and the fitting function. According to the invention, the tetanus human immune globulin national standard product is used as a calibration product to be calibrated on a biochemical analyzer, and then the tetanus antibody titer of a test sample is determined by using the tetanus antibody detection reagent solution, so that the titer of the test sample can be rapidly and accurately determined, and the cost is far lower than that of a mouse method.

The application is suitable for the field of medical equipment, and discloses a sample analyzer. The sample analyzer comprises a machine body, a first loading component, a first buffer component, a first transfer component, an output component, a detection component and a controller. The first buffer component is used for buffering first consumables to be used and coming from the first loading component; the controller is configured to: in the process that the first loading component performs a task of loading the first consumables, control the detection component to perform at least part of a process in a sample detection item on a sample by using the first consumables buffered by the first buffer component, control the output component to output first operation prompt information according to a first residual time in which the first consumables buffered by the first buffer component can support the detection component to perform the sample detection item, so as to remind to complete the operation of loading the first consumables in the first loading component within the first residual time. The sample analyzer can meet the design requirements of loading consumables without stopping and small biological safety risk under a small volume.

The invention discloses a pasteurella multocida capsule mutant strain and application thereof, and belongs to the field of microorganisms, the pasteurella multocida capsule mutant strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20242234. A mouse immune challenge test and a detection test of a Pm strain antibody detection indirect ELISA method show that the RPmF2019s has a better immune effect and has more advantages as a coating antigen of the Pm detection ELISA method.

The utility model discloses an integrated urine collecting cup which comprises a folding funnel, a handle is fixedly arranged on one side of the folding funnel, and the bottom of the folding funnel is connected with a small test tube in a sealing mode. The other end of the small test tube is hermetically connected with a knob cover, the lower end of the knob cover is hermetically connected with the large test tube, and the knob cover is used for controlling communication and separation between the small test tube and the large test tube; and a spare cover is also arranged on the end surface of the large test tube. A first rotating part and a second rotating part which are attached up and down are arranged in a knob cover, and a first notch and a second notch are formed in the first rotating part and the second rotating part respectively, so that connection and separation of test tube containers connected to the upper end and the lower end of the knob cover are controlled by rotating the knob cover; therefore, midstream urine or initial urine can be collected under the condition that urine collection is not interrupted, the risk that the urine spills to the hand of a patient is eliminated, the operation link of transferring the urine from a urine cup to a test tube is reduced, the biological safety risk is reduced, and the urine is convenient to take.

The invention belongs to the technical field of biology, and particularly relates to preparation and application of a swine A type FMDV VP1 antigen and single factor positive serum. The sequence of the expression gene of the swine A type FMDV VP1 antigen is as shown in SEQ ID NO. 1. Through the integral technical scheme of combining sequence-driven conservative site screening, expression and immunological verification, the key problems of antigen mismatch, difficult recombinant protein expression, poor diagnostic reagent universality and the like in the prior art are solved; and the effectiveness and generalizability of the vaccine, the diagnostic reagent and the monitoring means in practical application are obviously enhanced.

The purpose of the present invention is to provide a method for detecting O-type foot-and-mouth disease virus, that is, on the basis of screening a group of RT-RAA primer probes for highly efficient and specific detection of O-type foot-and-mouth disease virus, RT-RAA technology and CRISPR / Cas13a technology are combined to achieve high sensitivity, high specificity and low complexity visual detection of FMDV-O clinical samples. The present invention also provides a detection method with simple operation, sensitive detection, visual results and low auxiliary equipment requirements, so as to improve the detection of O-type foot-and-mouth disease virus in the pig breeding industry, solve the key technical problem that it is difficult to diagnose FMDV at the disease and quarantine site due to the similar clinical symptoms of vesicular epidemics, and need to be sent to the laboratory for differential diagnosis, reduce biosafety risks, provide technical means, and provide a basis for early diagnosis and timely prevention and control of epidemics in grassroots veterinary departments, which is of great significance for preventing and controlling the occurrence and spread of the epidemic.

The application discloses a portable laboratory cold storage box with good protection, and belongs to the field of laboratory cold storage boxes. The portable laboratory cold storage box with good protection comprises a box body, a box cover hinged to the box body and locked to the box body through a lock buckle, a handle hinged to the position close to the top end of the side surface of the box body, and backpack straps fixed symmetrically on the side of the box body close to the hinged end of the box cover. An isolation frame is arranged in the box body, and the isolation frame divides the box body into three refrigeration cavities for placing ice blocks or ice bags, namely a bottom refrigeration cavity located at the bottom of the isolation frame. The portable laboratory cold storage box with good protection replaces the built-in refrigeration structure with an external cold source, significantly reduces the weight of the equipment, and cooperates with the combined design of the handle and the backpack straps to adapt to various scenes such as single-person operation, short-distance carrying or long-time carrying, and solves the problem that the traditional cold storage box is heavy and difficult to carry.

The invention discloses a totally-enclosed avian influenza H5, H7, H9 and universal virus quadruple detection kit. The kit comprises primers and probes of a universal avian influenza virus M gene, an H5 subtype avian influenza virus HA gene, an H7 subtype avian influenza virus HA gene, an H9 subtype avian influenza virus HA gene and a reference gene LconR. According to the invention, the ligand-dependent nuclear receptor corepressor protein is introduced as a reference gene, and the gene widely exists in mammals and birds and is very suitable for detection of multi-species infection epidemic diseases such as avian influenza. The internal reference can participate in nucleic acid extraction and amplification processes, strictly controls the quality of each detection step, fundamentally avoids false negative results, and significantly improves the reliability of detection results. The internal reference also expands the species application range of the detection method, so that avian influenza detection is not limited to poultry any more, and the method can be widely applied to all mammals possibly infected with avian influenza.

An integrated microbial reagent plate of a microfluidic channel integrally comprises seven parts, namely a self-sealing sample inlet (1), a tree-shaped fractal flow channel plate (2), reaction holes (3), an air bag compensation cavity (4), sponge (5), a pre-cutting groove reagent plate covering film (6) and a plate placing frame (7), and the standard inclination plate placing frame (7) is matched with the integrated microbial reagent plate for use. The integrated microbial reagent plate of the microfluidic channel is simple in structure and convenient to operate, the cost is greatly reduced, and the pollution risk can be avoided.

The application discloses a preparation method of a drug sustained-release hydrogel support and a three-dimensional osteogenic induction application of hiPSCs. Firstly, a CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere of a core-shell structure is prepared, then the freeze-dried GelMA powder is dissolved in a PBS solution containing a photoinitiator LAP, the CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere is added after stirring and cooling, and a drug-loaded hydrogel precursor is obtained; the precursor is injected into a mold and irradiated by a specific wavelength light source to initiate free radical polymerization of a methacryl group into glue, and a drug-loaded GelMA porous hydrogel support is obtained after demolding, freezing and drying; the drug-loaded GelMA porous support is covalently grafted with VN polypeptide through EDC / NHS chemical coupling to obtain a VN-GelMA drug-loaded porous hydrogel support. The application simulates the signal time sequence regulation law in the embryonic bone formation process and significantly improves the osteogenic differentiation efficiency and mineralization quality by constructing a biomimetic three-dimensional osteogenic microenvironment. The VN polypeptide enhances the specific recognition of stem cells and significantly improves the three-dimensional cell compatibility. The application can reduce the dependence on exogenous induction factors and is safe, low in cost and stable in induction.

The invention relates to the technical field of risk early warning, and discloses a high biosafety risk workshop liquid leakage alarm method and device, and the method comprises the steps: arranging a plurality of detection wires in a cofferdam, and enabling the detection wires to cover and exceed the orthographic projection area of a virus bioreactor, so as to monitor the possible virus liquid leakage condition; when virus liquid leakage is detected by the detection lead, liquid leakage signals are collected, processed and analyzed; a sound-light alarm is triggered to inform field operators and operators on duty in a central monitoring room to respond to potential safety risks in time; the sound-light alarm information is transmitted to a server of a central monitoring room, and the server is used for displaying the equipment state and storing and analyzing leakage alarm records. The system comprises a detection lead, a PLC controller, a sound-light alarm module and a central monitoring room. According to the invention, efficient monitoring and response of virus liquid leakage are realized, and the safety and scientificity of workshop management are improved. And a perfect monitoring and response mechanism is formed.

The invention discloses a preparation method of an absorbable composite material for an interface screw and the interface screw, comprising: S1, adding a premix 1 mixed with polylactic acid, part of polyethylene glycol and part of hydroxyapatite to the main feeding port of a double-side feeding twin-screw extruder; S2, adding a premix 2 mixed with polylactic acid-glycolic acid copolymer, part of polyethylene glycol and part of hydroxyapatite to the first side feeding port; S3, adding a premix 3 mixed with part of polyethylene glycol and part of hydroxyapatite to the second side feeding port, and preparing the mixture in a twin-screw extruder at a temperature of 180-220° C.; the weight ratio of hydroxyapatite in steps S1, S2 and S3 is (1-3): (1-4): 1; the weight ratio of polyethylene glycol in steps S1, S2 and S3 is (2-4): (1-3): 1. In this way, the formula components of the polylactic acid / HA composite material are simplified, and the processing performance of the polylactic acid / HA composite material is improved.

The utility model relates to a kind of storage tank and its automatic spading device.The automatic spading device includes: push plate assembly, it includes fixed part and push plate, at least two push plates are spaced apart along the length direction of the fixed part;Drive assembly, it includes guide rail, sliding member and driving part, sliding member is slidably connected with the guide rail, and is connected with the fixed part;The driving part is drivingly connected with the sliding member, to drive the sliding member moves along the guide rail.The utility model moves by cylinder drive push plate assembly, replaces artificial spading, improves loading efficiency and reduces manpower cost and labor intensity;At the same time, by setting multiple push plates, it is realized to increase the pushing area of push plate assembly when slider moves the same distance, to increase the single pushing capacity and pushing distance of push plate assembly, to further facilitate shorten loading time and improve tank fullness coefficient.In addition, automated operation reduces artificial contact, reduces biological safety risk, reduces additional disinfection demand brought by artificial operation, reduces operating cost.

The application discloses a light-weight large-flux sterilization transfer device and method for experimental animal facilities, and belongs to the technical field of disinfection and sterilization transfer, which comprises a sterilization cabin, a loading cabin and an unloading cabin, and the loading cabin and the unloading cabin are arranged on the two sides of the sterilization cabin respectively; the sterilization cabin comprises a plastic film and a supporting frame, and the inside of the supporting frame is provided with a circulating pipeline. The application has the beneficial effects that: the supporting frame and the airtight plastic film are adopted to construct the sterilization cabin, a large flexible light-weight airtight space is formed, the device is light and easy to assemble, the vaporized hydrogen peroxide pipe is injected into the heat-insulating circulating pipeline of the supporting frame, the heat loss of the vaporized hydrogen peroxide pipe can be avoided, the vaporized hydrogen peroxide can uniformly act on the surface of the object at a stable concentration through the setting of the air inlet hole and the air return hole in the sterilization cabin, the air exchange efficiency is improved, the sterilization effect is improved, and then the vaporized hydrogen peroxide which is not fully reacted can be returned through the circulating pipeline, so that the rapid circulation is realized.

The invention discloses a microcapsule and a preparation method thereof, and self-repairing polyurethane and a preparation method and application thereof, relates to the field of polyurethane materials, and solves the problems that the self-repairing process of the self-repairing polyurethane in the prior art depends on non-physiological stimulation conditions, the cytotoxicity is high, the biosafety is insufficient, and the application requirements in the field of biomedical instruments are difficult to meet. The invention discloses a preparation method of self-repairing polyurethane, which comprises the following steps: blending a prepolymer I, a prepolymer II and a solvent, adding an Arabic gum aqueous solution and a chain extender, and adding triethanolamine to obtain a suspension containing microcapsules; and washing, carrying out solid-liquid separation, and freeze-drying to obtain the microcapsule. Adding the microcapsules into the polyurethane dioxane solution, uniformly stirring, pouring into a mold, and heating or freeze-drying in vacuum to obtain the self-repairing polyurethane. The microcapsule provided by the invention has excellent biocompatibility, solvent resistance and mechanical properties, the self-repairing condition of the self-repairing polyurethane is mild, and the application requirements in the field of biomedical instruments are met.

The invention discloses a polydopamine (PDA)-coated polylactic acid-glycolic acid (PLGA) targeted sustained release microsphere (Hep-coated PLGA-PDA MPs) loaded with heparin sodium, which is characterized in that the polylactic acid-glycolic acid microsphere (Hep-coated PLGA-PDA MPs) loaded with the drug heparin sodium is an inner core microsphere, and the outer part of the microsphere is also coated with a PDA layer. The invention also discloses a preparation method of the Hep-coated PLGA MPs, the microspheres have no burst release phenomenon within 2 hours of drug release, the release proportion of heparin sodium is 45.7-57.2% in 24 hours, and the microspheres have the characteristic of slow release. The microsphere disclosed by the invention can be used for developing an intravenous injection sustained-release agent for targeted therapy of thrombus.

A kind of secondary polishing type rodent breeding cage box, it is related to barrier environment experimental animal technical field, including cage box, the inside of the cage box is equipped with inner cavity, secondary polishing box main body is installed in the inner cavity, and secondary polishing box main body is vertically inserted with cage box and is limited, the upper end surface of secondary polishing box main body is equipped with integrally formed flange, for separating secondary polishing box main body with cage box, one side of the cage box is provided with clamping mechanism, for reinforcing installation to secondary polishing box main body, the present scheme improves animal daily feeding efficiency, while it can minimize staff operation and working hours input, avoid the problem of secondary pollution in cage box cleaning and disinfection process.