Closed full-automatic nucleic acid extraction and detection system based on CRISPR technology

Abstract

The invention discloses a closed full-automatic nucleic acid extraction and detection system based on a CRISPR technology. The closed full-automatic nucleic acid extraction and detection system comprises a bag type biological chip and a control detector, wherein the bag type biological chip comprises a nucleic acid extraction cavity, a No.1 cleaning liquid cavity, a No.2 cleaning liquid cavity, aNo.3 cleaning liquid cavity, an eluent cavity, a waste liquid cavity, an amplification cavity and a CRISPR detection cavity which are respectively used for storing various liquids required by nucleicacid extraction and detection, and the cavities are connected through micro-channels; and the control detector comprises a push rod controller, a controllable alternating electromagnet and a fluorescence detector, wherein the push rod controller is used for controlling a flow direction of liquid, the controllable alternating electromagnet is used for stirring and adsorbing magnetic beads, and thefluorescence detector is used for CRISPR luminescence detection of nucleic acid. According to the closed full-automatic nucleic acid extraction and detection system based on the CRISPR technology, nucleic acid cross contamination during detection can be avoided, in addition, the bag type biological chip and the control detector are relatively separated, a detection sample can be rapidly replaced,and practical detection application is facilitated.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection, in particular to a closed automatic nucleic acid extraction and detection system based on CRISPR technology. Background technique [0002] Nucleic acid detection is the gold standard for identifying biological species and an important means for the detection and identification of viruses, bacteria and other microorganisms. As a gene editing technology, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology can accurately identify nucleic acid fragments and can be widely used in nucleic acid detection. In nucleic acid detection, it is usually necessary to extract nucleic acid, concentrate it and then perform nucleic acid detection, which helps to improve the sensitivity of nucleic acid detection. [0003] At present, commercial nucleic acid extraction and detection equipment are all open, which is easy to cause cross-contamination of nucleic acid, and there are still r...

AI Technical Summary

Problems solved by technology

[0003] At present, commercial nucleic acid extraction and detection equipment are all open, which is easy to cause cross-contamination of nucleic acid, and there are still risks to human safety when detecting pathogenic bacteria or viruses

On the other hand, currently commonly used nucleic acid detection is based on PCR amplification, isothermal amplification or gene sequencing, but the above detection methods take a long time

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