Closed full-automatic nucleic acid extraction and detection system based on CRISPR technology

A detection system, fully automatic technology, applied in the determination/inspection of microorganisms, laboratory containers, bioreactors/fermenters for specific purposes, etc., can solve the problems of nucleic acid cross-contamination, human safety risks, and long time. , to achieve rapid replacement, avoid nucleic acid cross-contamination, and reduce biosafety risks

Pending Publication Date: 2020-08-25
SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI +1
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Problems solved by technology

[0003] At present, commercial nucleic acid extraction and detection equipment are all open, which is easy to cause cross-contamination of nucleic acid, and there are still risks to human safety when detecting pathog

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  • Closed full-automatic nucleic acid extraction and detection system based on CRISPR technology

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[0016] The present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be emphasized that the following description is only exemplary and is not intended to limit the scope and application of the present invention.

[0017] This embodiment provides a closed automatic nucleic acid extraction and detection system based on CRISPR technology, which includes the following steps: pre-installing the lysis solution and magnetic beads in the nucleic acid extraction chamber; pre-preparing the cleaning solutions No. 1, No. 2 and No. 3 respectively Installed in No. 1, No. 2 and No. 3 cleaning solution chambers; pre-loaded the eluent in the eluent chamber; pre-loaded the amplification solution in the amplification chamber; pre-loaded the CRISPR reaction solution in the CRISPR detection chamber , Constructed into a kit for nucleic acid extraction and detection.

[0018] First, add the target sampl...

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Abstract

The invention discloses a closed full-automatic nucleic acid extraction and detection system based on a CRISPR technology. The closed full-automatic nucleic acid extraction and detection system comprises a bag type biological chip and a control detector, wherein the bag type biological chip comprises a nucleic acid extraction cavity, a No.1 cleaning liquid cavity, a No.2 cleaning liquid cavity, aNo.3 cleaning liquid cavity, an eluent cavity, a waste liquid cavity, an amplification cavity and a CRISPR detection cavity which are respectively used for storing various liquids required by nucleicacid extraction and detection, and the cavities are connected through micro-channels; and the control detector comprises a push rod controller, a controllable alternating electromagnet and a fluorescence detector, wherein the push rod controller is used for controlling a flow direction of liquid, the controllable alternating electromagnet is used for stirring and adsorbing magnetic beads, and thefluorescence detector is used for CRISPR luminescence detection of nucleic acid. According to the closed full-automatic nucleic acid extraction and detection system based on the CRISPR technology, nucleic acid cross contamination during detection can be avoided, in addition, the bag type biological chip and the control detector are relatively separated, a detection sample can be rapidly replaced,and practical detection application is facilitated.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection, in particular to a closed automatic nucleic acid extraction and detection system based on CRISPR technology. Background technique [0002] Nucleic acid detection is the gold standard for identifying biological species and an important means for the detection and identification of viruses, bacteria and other microorganisms. As a gene editing technology, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology can accurately identify nucleic acid fragments and can be widely used in nucleic acid detection. In nucleic acid detection, it is usually necessary to extract nucleic acid, concentrate it and then perform nucleic acid detection, which helps to improve the sensitivity of nucleic acid detection. [0003] At present, commercial nucleic acid extraction and detection equipment are all open, which is easy to cause cross-contamination of nucleic acid, and there are still r...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/00
CPCB01L3/5027C12Q1/6806C12Q1/6844C12Q2523/308C12Q2521/327C12Q2525/161C12Q2563/107C12Q2565/629
Inventor 陆卫郑伟波王少伟刘方武孙聊新丁昆江舸张涛黄行许孙浩袁永春张寅
Owner SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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