142 results about "Hplc fld" patented technology

Microfluidic assay detectors and microfluidic assay detection methods are disclosed. A microfluidic chip is coupled to a light emitting device, emission filters and excitation filters. Excited fluorescent light is detected by a camera and a lens. The correspondent reading allows parallel detection of features such as antigens and biomarkers. A microfluidic filter and related methods are also disclosed. The filter can be used with on-chip fluid filtration such as whole blood filtration for microfluidic blood analysis. The filter is able to filter the necessary volume of fluid and in particular blood in an acceptable time frame.

An apparatus and method for medical practitioners to detect the presence of abnormal cells including cancerous and pre-cancerous cells by using a transport capsule containing an imaging apparatus including UV sources and fluorescence detectors for obtaining images and fluorescence data of biological cells and tissue. The method includes the steps of scanning biological tissue using an ultra-violet (UV) source to obtain fluorescence data, transferring fluorescence data and/or images using a radio frequency (RF) or other suitable means to a personal computer (PC) system, analyzing the image and/or fluorescence data in the PC, identifying -tissues with precancerous and cancerous cells, and optionally determining their precise location, and assessing the accuracy of the calculated fluoroscopic images.

Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.

System and method for detecting and measuring chemical perturbations in a sample. The system and method are useful for non-invasive pH monitoring of blood or blood products sealed in storage bags.

A blood analyzer, comprising a sample preparing part; a flow cell; a light source; a scattered light detector for detecting scattered light from a measurement sample irradiated by the light source; a fluorescence light detector comprising an avalanche photo diode for detecting fluorescence light from the measurement sample irradiated by the light source; a signal processing part for processing a first detection signal from the scattered light detector and a second detection signal from the fluorescence light detector, wherein the signal processing part reduces high frequency noise included in an amplified second detection signal; and a analysis part for classifying white blood cells in the blood into groups based on the first and the second detection signals processed by the signal processing part is disclosed. A blood analyzing method is also disclosed.

A system that incorporates teachings of the present disclosure may include, for example, an apparatus having a collimator having at least one aperture and a fluorescence detector. The collimator can be positioned next to a compound. The compound can emit fluorescence X-rays when impacted by an X-ray beam generated by an X-ray source. The collimator can absorb at least a first portion of the fluorescence X-rays emitted by the compound and release at least a second portion of the fluorescence X-rays at the at least one aperture. The second portion of the fluorescence X-rays released by the at least one aperture have known directional information based on a position of the collimator. The fluorescence detector can detect the second portion of the fluorescence X-rays released by the at least one aperture. A three-dimensional (3-D) rendering of an elemental distribution of the compound can be determined from the fluorescence X-rays detected and the directional information. Additional embodiments are disclosed.

The invention discloses a rapid detection method for production process quality control of yellow wine, belonging to the technical field of food analysis and food safety, comprising the following steps: (1) carrying out dilution and determining capacity on a yellow wine sample with absolute ethyl alcohol; (2) mixing the diluted yellow wine sample with a 9-hydroxy-xanthene solution in a hydrochloric acid solution and reacting for 60 min at room temperature; and (3) carrying out component separation on the derivative yellow wine sample with high performance liquid chromatography, and calculating the content of urea and ethyl carbamate (EC) in the sample with the external standard method. According to the invention, by setting different parameters at different time periods for a fluorescence detector, the technical difficulty of simultaneously detecting the content of EC and urea in yellow wine is solved, a HPLC-FLD method for simultaneously detecting EC and urea in yellow wine is established, thus a rapid, convenient, accurate and effective monitoring method for yellow wine safety production is provided.

A measurement apparatus and method are provided for determining the material composition of a sample. An X-ray fluorescence detector (412) detects fluorescent X-rays coming from said sample under irradiation with incident X-rays. A laser source (301) is adapted to produce a laser beam. Focusing optics (302) focus said laser beam into a focal spot on a surface of said sample. An optical sensor (312) detects optical emissions coming from particles of said sample upon being exposed to said laser beam at said focal spot. A gas administration subsystem (104, 105, 106, 107, 108) is adapted to controllably deliver gas to a space (101) around said focal spot.

A liquid chromatograph, comprising a first analysis column, which separates a component in a sample guided by a first mobile phase; first detection device for detection of the component; a fractionation flow path, which fractionates and holds in an isolation portion the component detected by the first detection device; a trap flow path, which sends the component held in the isolation portion into a trap column, and causes capture and concentration of the component in the trap column; a second analysis column, which separates the component which has been captured and concentrated in the trap column and is eluted from the trap column by a second mobile phase; and second detection device for detection of the component separated in the second analysis column, wherein the first and second detection devices have a detector selected from a group consisting of a photodiode array detector, an infrared detector, a radioisotope detector, and a fluorescence detector.

The invention discloses a method for measuring main phenolic compounds in electronic cigarette liquid. The method comprises the steps of preparing a sample solution to be measured by an ultrasonic extracting method, preparing a mixed standard working solution with give concentration gradients, analyzing the solution by a high-efficient liquid-phase chromatography and a fluorescent detector, and quantizing by an external standard method. The result shows that the method is simple in preprocessing, simple to operate, short in analysis time, high in sensitivity, good in recovery rate and precision and suitable for measurement on the content of phenolic compounds in the electronic cigarette liquid.

A method for detecting zearalenone toxin in traditional Chinese medicines in different matrices, including extraction, purification and detection of Chinese medicine samples, said samples are extracted homogeneously at high speed with methanol-water (80:20, V/V), pH7. 0 Tween-PBS buffer solution dilution, glass fiber membrane filtration, immunoaffinity column purification. The determination method includes: high performance liquid chromatography-fluorescence detection method, high performance liquid chromatography-diode array detection method determination, spectrum and high performance liquid chromatography-mass spectrometry confirmation. Liquid chromatography conditions are: mobile phase ratio methanol: acetonitrile: water (8:46:46, V/V/V), fluorescence detector wavelength: excitation wavelength: 270nm, emission wavelength: 440nm; diode array detector wavelength: 236nm . Liquid quality conditions: Methanol-0.1% ammonium acetate water (75:25, V/V) as mobile phase, atmospheric pressure chemical ionization positive ion mode, drying gas flow rate 6.00L/min, drying gas temperature 340°C, fragmentation voltage 150V, Full scan mode scan, scan range 200-400m/z. The invention has the characteristics of accuracy, precision, reliability and the like.

A handheld fluorescence detector that includes a handheld data processing system and a UV light source connected to the data processing system is disclosed. The UV light source illuminates an object to be scanned with light having a UV illumination wavelength. A safety mechanism inhibits the light from the UV light source from reaching an eye of a person in the vicinity of the UV light source at an intensity that would damage the eye. A fluorescence detector senses fluorescent light generated by the object in response to the illumination. The fluorescence detection can utilize a photodetector or a human observer. The detector can be included in a cellular telephone or PDA. Safety mechanisms that utilize baffles or total internal reflection to protect the user are described. In addition, interlock mechanisms that prevent the UV light source from being activated when no object is present can be incorporated.

A fluorescence microscopy imaging system is used for detecting a fluorescence signal of a sample, and includes a module for detecting fluorescence and a module for focusing control. The module for detecting fluorescence includes a fluorescence excitation light source generator (FELSG) and a fluorescence detector. The FELSG is capable of generating an excitation light beam having a first wavelength to excite the sample to emit fluorescence. The fluorescence detector is used to read the fluorescence signal of the sample. The module for focusing control generates a servo light beam having a second wavelength. A servo light beam reflecting film disposed on an observation plane is used to reflect the servo light beam. A return beam signal is analyzed using a focusing detection method. An actuator is used to move the objective for focusing, so as to enable the fluorescence excitation light beam to excite the sample to emit fluorescence.

The invention discloses an HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco. The HPLC-FLD pre-column derivatization method comprises the following steps: performing water-bath ultrasonic extraction on a tobacco powder sample by use of a methanol solution, filtering, diluting the filtrate and enabling the diluted filtrate to pass through an immunoaffinity column, performing twice drip washing on the immunoaffinity column by use of pure water, next, eluting four aflatoxins enriched on the immunoaffinity column by use of methanol, performing blow-drying on the eluted solution by use of nitrogen, then, adding trifluoroacetic acid and normal hexane for derivatization at the constant temperature being 45 DEG C for 40 minutes, adding a small quantity of methanol solution and carrying out HPLC-FLD analysis on the taken low-layer solution. The HPLC-FLD pre-column derivatization method can be applied to simultaneous determination of the aflatoxins B1, B2, G1 and G2 in tobacco and tobacco products, and is capable of completing determination in 20 minutes; the four targets can be well separated, the linear relation is good and the detection limit is low; besides, the HPLC-FLD pre-column derivatization method has good recovery rate and reproducibility, and is suitable for detecting the aflatoxins in tobacco and tobacco products.

Utilizing a linear relationship between concentration of formaldehyde and CdTe quantum dot fluorescence intensity, the invention provides, designs and develops a novel fluorescence detector for determination of formaldehyde content of water. The detector mainly comprises a peristaltic pump, a light source, a sample pool, a detector, and an output display, can realize fast and accurate detection of formaldehyde content of water, and has advantages of simple structure, low cost, small volume, low energy consumption, convenient operation, high sensitivity and wide detection range of detection.

The invention provides a method for measuring the contents of tyrosine, tryptophan and 5-hydroxytryptamine in blood serum by adopting a high-efficiency liquid phase chromatography-fluorescence method. The method is accurate and rapid, and comprises the following steps of: removing proteins from blood serum with perchloric acid with the concentration of 5 percent and detecting by adopting a high-efficiency liquid phase chromatography technology, wherein a chromatography condition is that: a chromatography column is an Atlantis C18 column of the specification of 4.6 millimeters*150 millimeters ID; mixing 0.1 mol/L of KH2PO4 with methanol in the volume ratio 85:15 at the flow rate 1.0 ml/min to obtain a mixed solution serving as a moving phase, wherein the excitation wavelength and the emitting wavelength of a fluorescence detector are 228 nanometers and 306 nanometers respectively within 0-4minutes, 278 nanometers and 338 nanometers within 4-7.5 minutes and 285 nanometers and 353 nanometers after 7.5 minutes; and adjusting the wavelengths within a specific period of time to perform separate detection on the tyrosine, the 5-hydroxytryptamine and the tryptophan.

The invention belongs to the technical field of nucleic acid detector equipment, and particularly relates to an isothermal amplification nucleic acid detector. The detector comprises a shell, a constant temperature control module, an optical detection module and a control module, wherein the shell comprises an outer shell and a shell upper cover; the constant temperature control module comprises a test tube bearing plate, a heat conducting plate, a heating plate, a cooling fin, a cooling fan and a temperature sensor; the optical detection module comprises a light source generator, a lens, an optical filter, a total reflection mirror and a fluorescence detector; and the control module comprises a control circuit and a data processor. The instrument can quickly perform nucleic acid amplification detection in real time at a constant temperature, has the characteristics of low energy consumption, small size, convenience in carrying, simplicity in operation and the like, and can be applied to multiple fields such as nucleic acid molecule laboratories, medical diagnosis, field detection and the like.

An ultra small fluorescence detector capable of detecting in real time reaction undergoing in a micro chamber having a predetermined volume and disposed on a microfluid chip is provided. The fluorescence detector for detecting in real time PCR amplification undergoing in the microfluid chip having a micro chamber with a predetermined volume includes a light source generating an excitation beam, a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber, a first detector, and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber to the first detector. Accordingly, the fluorescence detector is designed such that light emitted by a light source is focused between a first mirror and an objective lens. Therefore, the spot size of an excitation beam transmitted by the objective lens is largely formed so that the excitation beam can be irradiated on the whole micro chamber of the microfluid chip, thereby detecting a fluorescent beam on a broader area.

The invention provides a method for determining the amount of residual florfenicol in an aquatic product by using a high-efficiency liquid chromatogram fluorescence method. In the method, florfenicolin an aquatic product sample is extracted by ethyl acetate, degreased by normal hexane, concentrated and purified by C18 solid extraction column, determined by a liquid chromatograph and a fluorescence detector and quantitated by an external standard method. The method can determine the residual florfenicol in the aquatic product quickly, sensitively and accurately.

The invention relates to a method for analyzing and determining four heterocyclic pesticides in a water sample, and belongs to the field of pesticide detection. The method specifically comprises the following steps: preparing a metal organic framework material MIL-53 and an MIL-53 membranes; and analyzing four phenoxy carboxylic acid pesticides in the water sample by using solid-phase extraction via the metal organic framework material MIL-53 in combination of high performance liquid chromatography. The organic framework material MIL-53 with high adsorption efficiency for the four phenoxy carboxylic acid pesticides is used, and serves as a membrane solid-phase extraction material, a high performance liquid chromatography-ultraviolet fluorescence detector is adopted, the analysis method ofthe four phenoxy carboxylic acid pesticides in the water sample is established, the method is simple and convenient to operate, the analysis time and cost are greatly reduced, the extraction effect isgood, the analysis result is accurate, and the reproducibility is good; and raw materials are cheap and easy to obtain, the material preparation process is simple, the reaction conditions are mild, the application environment is friendly, and the market prospect is wide.

In a fluorescence-microscopic method of examining a sample with high spatial resolution, the sample is first cooled to a temperature of below 5° C.; next the cooled sample is transferred out of a ground state into a fluorescent state within an area captured by a detector using an excitation beam of light; next the cooled sample is subject to de-excitation of excited molecules by stimulated emission in the area captured by the detector, except at desired measuring points, using an de-exciting beam of light, the de-exciting beam of light having a spatial intensity distribution comprising a zero point located at the desired measuring points, and the excited sample being transferred back into its ground state by the de-exciting beam of light; and then fluorescence light spontaneously emitted by the cooled sample is measured with the detector, the detector detecting fluorescence light that is emitted only from the measuring points.

The invention discloses a high performance liquid chromatography-fluorescence detection method for 3-MCPD. The method comprises the following steps: (1) treating a 3-MCPD aqueous solution, and cracking the 3-MCPD into chloroacetaldehyde; (2) removing excessive periodate, and eliminating side reaction; (3) carrying out fluorescence derivation reaction, namely enabling the chloroacetaldehyde to react with a fluorescence derivation reagent so as to generate a target material with a fluorescence effect; and (4) performing HPLC-FLD measurement, namely separating the target material, and quantifying the 3-MCPD according to the chromatographic peak area, wherein the fluorescence derivation reagent refers to an o-amino binary N-heterocyclic compound. According to the method disclosed by the invention, a type of novel fluorescence derivation reagents with convenient and readily available materials, high selectivity, high fluorescence efficiency and high hydrophobicity can be used for high performance liquid chromatography-fluorescence detection of 3-MCPD, so that the target product has a good retention behavior in the reversed phase liquid chromatography, and the accuracy and sensitivity of the test method are improved.