14 results about "Hplc fld" patented technology

The invention relates to the technical field of bacterial detection, in particular to a bacterial drug sensitivity detection assembly based on an ATP luciferase method and a using method.The bacterial drug sensitivity detection assembly comprises a supporting assembly, a sampling assembly, an ATP fluorescence detector and a 96-hole drug sensitivity plate, and a sealing block is arranged at the top of the inner wall of the ATP fluorescence detector; the supporting assembly comprises a base, a controller is arranged on one side of the base, and an electric push rod is arranged in the middle of the upper surface of the base. The device effectively realizes detection of bacterial drug sensitivity of a sample, is high in detection precision and good in detection effect, can also mix and stir the sample in the 96-hole drug sensitive plate, and avoids precipitation of bacteria in the 96-hole drug sensitive plate due to long-time standing; the sampling quantity of samples can be accurately detected, and the accuracy and the stability of subsequent detection are ensured; when the sample in the sampling tube is mixed with the reaction liquid, the flowing efficiency of the sample can be ensured, and the problems of high liquid feeding difficulty, poor mixing effect and the like caused by negative pressure are avoided.

The invention discloses a fluorescence detector for analyzing ultracentrifugation. The fluorescence detector comprises an optical system, a metal panel and a vacuum stepping motor system, the optical system is fixed on the metal panel and is used for collecting a fluorescence signal generated by laser excitation of a sample in the ultracentrifugal sample pool through the first focusing lens; the vacuum stepping motor system comprises a sliding table, a radial stepping motor and an axial stepping motor, the metal panel is installed on the sliding table, the radial stepping motor drives the sliding table to move, and radial scanning is achieved; the axial stepping motor is connected with the first focusing lens and drives the first focusing lens to move up and down in the axial direction, and focusing adjustment is achieved. Through the design of an optical system, vacuum integration design and a vacuum stepping motor system, high-precision and stable fluorescence signal acquisition can be realized, and the device and the method can be widely applied to fluorescence detection for ultracentrifugation analysis.

Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.

This paper provides a method for detecting low concentrations of amphoteric compounds in a sample, comprising: (a) preparing an analytical sample mixture for capillary isoelectric focusing (CIEF) detection using the sample; and (b) detecting the analytical sample mixture using a fluorescence detector via a CIEF system; wherein the concentration of the low concentration amphoteric compound in the analytical sample mixture is less than 0.5 μg / μL. This method is more resistant to background noise interference and can sensitively detect and accurately quantify low concentrations of amphoteric compounds present in the sample.

The invention discloses a fluorescent algae analysis system and method, electronic equipment and a storage medium. The fluorescent algae analysis system comprises a light source driving circuit, a turbidity light source, a plurality of fluorescent light sources, a reference detector, a fluorescence detector, a turbidity detector and a data calculation module, the light source driving circuit is respectively connected with the turbidity light source and each fluorescent light source and is used for driving the turbidity light source and each fluorescent light source to work; the reference detector is used for detecting light intensity data of the turbidity light source or setting the fluorescence light source; the turbidity detector is used for detecting the turbidity of a water body to be detected; the fluorescence detector is used for detecting data of excited fluorescence with different intensities; the data calculation module is used for calculating the turbidity data and the fluorescence data to obtain algae analysis data. According to the fluorescent algae analysis system and method, the electronic equipment and the storage medium provided by the invention, the data stability and accuracy can be improved, and the influence of turbidity on a measurement result is eliminated.

The invention relates to an optical flow cytometer for fluorescence and scatter measurements, comprising: - a non-coherent light source intended to generate an illumination beam; - a beam truncation device comprising at least a first passage allowing a first portion of the illumination beam having a first divergence to pass, the first portion being dedicated to scatter measurements, and a second passage allowing a second portion of the illumination beam having a second divergence to pass, the second portion being dedicated to fluorescence measurements, the second divergence being greater than the first divergence, the first passage and the second passage being separated by a region of the beam truncation device blocking the illumination beam; - at least one focusing lens for focusing the first portion and the second portion of the illumination beam to a flow cell comprising an optical interrogation zone; - a flow cell intended to contain particles flowing through the optical interrogation zone; - a scatter detector for receiving light scattered from the first portion of the illumination beam when a particle passes through the optical interrogation zone; - a fluorescence detector for receiving fluorescence emitted by a particle passing through the optical interrogation zone.

The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.

The invention belongs to the technical field of analytical chemistry, and discloses an analysis method for determining the dissolution amount of HMDI in a medical instrument containing a polyurethane material, and the method comprises the following specific steps: 1, preparing a solution, namely preparing a mobile phase, a diluent, a derivatization reagent, a derivative solvent, a standard stock solution, a series of standard curve solutions, a process blank solution and an accuracy solution; the specific analysis method for the dissolution amount of the HMDI in the medical instrument containing the polyurethane material is established, the blank in the prior art is filled, the problem that the HMDI residue detection in the medical instrument industry lacks a unified standard is solved, and a reliable technical means is provided for product safety evaluation; meanwhile, 9-(methylaminomethyl) anthracene is adopted as a derivatization reagent and can be subjected to a specific reaction with the HMDI to generate a stable fluorescent derivative, and a fluorescent detector is combined for detection, so that the sensitivity of the method is remarkably improved, and the detection requirement of the dissolution amount of the low-concentration HMDI can be met.

The invention provides a method for detecting trace estradiol in a short time based on combination of dynamic oscillation extraction and HPLC-FLR (High Performance Liquid Chromatography-Flash Receptor), which comprises the following steps: sample pretreatment: putting a non-sheared drug coated sample into a container, adding trichloromethane, sealing, and carrying out dynamic oscillation extraction; the extraction process comprises at least one extraction period; tedious processes are reduced, and the extraction efficiency of target components with trace concentration and tight combination is remarkably improved. Performing chromatographic detection: analyzing the extracting solution by adopting a high performance liquid chromatography-fluorescence detector coupling technology; wherein the excitation wavelength of the fluorescence detector is 282nm, and the emission wavelength of the fluorescence detector is 315nm; mobile phases are water and acetonitrile. And other components do not fluctuate, so that the detection sensitivity and specificity are remarkably improved. According to the method disclosed by the invention, accurate and stable determination of estradiol in a concentration range of mu g / mL in a medicine coating is realized, so that strict quality control requirements are met.

According to some embodiments, a micro-Raman spectrometer includes a stage configured to hold a sample on a surface of which a fluorescent material is applied. The micro-Raman spectrometer includes a laser optical system configured to irradiate the sample with laser light. The micro-Raman spectrometer includes a Raman scattered light detector configured to detect Raman scattered light emitted from the sample. The micro-Raman spectrometer includes a fluorescence detector configured to detect fluorescence emitted from the fluorescent material. The micro-Raman spectrometer includes an analyzer configured to analyze a Raman signal of the Raman scattered light detector and a fluorescence signal of the fluorescence detector. The micro-Raman spectrometer includes a controller connected to the analyzer and configured to control the stage and the laser optical system.

The invention relates to the technical field of liquid chromatographs, in particular to a detection cell and a fluorescence detector. The detection pool comprises a pool body assembly, the pool body assembly is provided with an exposure area, a liquid inlet and a light inlet, the liquid inlet and the light inlet are formed in the two opposite ends of the exposure area in the axial direction, and the liquid inlet is used for allowing sample liquid to flow towards the exposure area; the light inlet is used for inserting an optical fiber, and light of the optical fiber is totally reflected in the exposure area. According to the detection pool, the light inlet of the optical fiber is opposite to the liquid inlet of the sample liquid, so that the liquid separation gap of the sample liquid flowing into the liquid inlet is eliminated, the diffusion volume of the liquid in the exposure area is eliminated, the influence of the liquid separation gap on the exposure area is eliminated, the peak shape broadening or trailing of a fluorescence analysis spectrogram is avoided, and the detection accuracy is improved. The peak shape and repeatability of the analysis spectrogram can be improved, so that the accuracy of an analysis result is improved.

The utility model relates to the technical field of biological detection, in particular to a fluorescence detection device based on quantum dots. The fluorescence detection device based on the quantum dots comprises a workbench, a side plate and a stand column, the side plate and the stand column are fixedly installed on the upper surface of the workbench, a fluorescence detector is fixedly installed on the side plate, two sets of ring frames are installed on the stand column, integrating spheres are fixedly installed on the two sets of ring frames, a light inlet pipe is installed at the top ends of the integrating spheres, and a light outlet pipe is installed at the top end of the light inlet pipe. A laser generator is installed at the top end of the light inlet pipe, a detection pipe is communicated with the side, close to the side plate, of the integrating sphere, the detection pipe is communicated with the detection end of the fluorescence detector through a guide pipe, a sample injection blind pipe is installed at the bottom end of the integrating sphere, and a sample injection groove is formed in the side, facing the stand column, of the sample injection blind pipe; and an automatic sample injection assembly matched with the sample injection groove is mounted on the stand column. The fluorescence detection device based on quantum dots provided by the utility model has the advantages of high detection efficiency and accurate detection.

The utility model discloses a kind of transferable antibody detection devices, it is related to antibody detection technical field, including bottom plate, detection component, control component and refrigeration storage component, the refrigeration storage component is fixedly installed at the top of bottom plate, the refrigeration storage component includes refrigerator, fixed plate, battery box and temperature indicating meter, the detection component includes fluorescence detector and wiring hole, the control component is fixedly installed at the top of detection component, the control component includes controller and control panel.The utility model uses the charger of adaptation and charging hole connection can charge battery box, battery box can be powered for refrigerator, it is convenient to long-distance transport antibody sample, without external power supply, improve the convenience when using, temperature indicating meter is convenient for staff to adjust the refrigeration temperature inside refrigerator, moving wheel is fixedly installed at the bottom of bottom plate, moving wheel is convenient for staff to transport a large number of test tubes once, improve work efficiency.

The invention belongs to the technical field of medicine quality control, and relates to a method for detecting a rhizoma atractylodis macrocephalae medicinal material in a five-ingredient spleen-tonifying mixture based on UPLC-FLR. The detection method of the rhizoma atractylodis macrocephalae medicinal material in the five-ingredient spleen-tonifying mixture comprises the following steps: mixing the five-ingredient spleen-tonifying mixture with a solvent, centrifuging, collecting supernate, air-drying the solvent in the supernate, adding the solvent for redissolving, and filtering to obtain a test solution; analyzing the test solution by adopting ultra-high performance liquid chromatography with a fluorescence detector; and determining that the rhizoma atractylodis macrocephalae medicinal material is detected in the five-ingredient spleen-tonifying mixture by using a chromatographic peak with the same retention time as that in the reference medicinal material. A mobile phase A of the ultra-high performance liquid chromatography is acetonitrile-phosphoric acid, and a mobile phase B of the ultra-high performance liquid chromatography is water-phosphoric acid; the excitation wavelength of the fluorescence detector is 270-290 nm, and the emission wavelength of the fluorescence detector is 440-460 nm. Through a selective excitation mechanism of a fluorescence detector in UPLC-FLR, matrix interference of non-fluorescent components in a compound is avoided, and detection of trace bighead atractylodes rhizome is realized.