58 results about "Hplc fld" patented technology

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

The invention relates to a method for detecting the content of amino acid in a pesticide through pre-column derivatization liquid chromatography, and relates to the technical field of chemical analys.The method comprises the steps that a pesticide sample to be detected is extracted, and a solution containing amino acid is obtained; then, under the optimized alkaline pH and heating conditions, 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole (NBD-F) is adopted as a derivatization reagent to be subjected to a pre-column derivatization reaction with amino acid, and a stable high-fluorescence NBD-F derivative is formed; injecting the derivative liquid into a high performance liquid chromatography system provided with a phenyl bonded phase chromatographic column, and efficiently separating the NBD-F derivative by utilizing pi-pi interaction and hydrophobic interaction of the phenyl column; and finally, detecting and quantifying through a fluorescence detector. Through the synergistic effect of NBD-F derivation and the phenyl column, the separation selectivity and the detection sensitivity are remarkably improved, and the reaction efficiency and the derivative stability are guaranteed through optimized derivation conditions.

The invention discloses a fluorescence detector based on an off-axis parabolic mirror, and belongs to the technical field of liquid chromatographic analysis, the fluorescence detector comprises a light source light path, an excitation monochromatic light path, a flow cell light path, a fluorescence collection light path, a fluorescence emission light path and a detector which are arranged in sequence, and the light source light path generates and converges ultraviolet-visible light; the converged ultraviolet-visible light is converted into monochromatic light with different wavelengths through the monochromatic light excitation path, the monochromatic light irradiates a to-be-detected sample in the flow cell light path to generate fluorescence, the fluorescence collection light path collects and converges the fluorescence, the converged fluorescence is divided into monochromatic fluorescence with different wavelengths through the fluorescence emission light path, and the monochromatic fluorescence is finally detected by the detector. The light source light path and the fluorescence collection light path both adopt off-axis parabolic reflectors to replace lenses, so that the influence of chromatic aberration is eliminated, and the utilization rate of optical energy is improved; the two off-axis parabolic reflectors are matched with each other, so that high spectral resolution is realized, and a simulation imaging light spot is less than 20 microns.

The invention relates to the technical field of bacterial detection, in particular to a bacterial drug sensitivity detection assembly based on an ATP luciferase method and a using method.The bacterial drug sensitivity detection assembly comprises a supporting assembly, a sampling assembly, an ATP fluorescence detector and a 96-hole drug sensitivity plate, and a sealing block is arranged at the top of the inner wall of the ATP fluorescence detector; the supporting assembly comprises a base, a controller is arranged on one side of the base, and an electric push rod is arranged in the middle of the upper surface of the base. The device effectively realizes detection of bacterial drug sensitivity of a sample, is high in detection precision and good in detection effect, can also mix and stir the sample in the 96-hole drug sensitive plate, and avoids precipitation of bacteria in the 96-hole drug sensitive plate due to long-time standing; the sampling quantity of samples can be accurately detected, and the accuracy and the stability of subsequent detection are ensured; when the sample in the sampling tube is mixed with the reaction liquid, the flowing efficiency of the sample can be ensured, and the problems of high liquid feeding difficulty, poor mixing effect and the like caused by negative pressure are avoided.

The invention discloses a preparation method of an anti-ultraviolet light absorption coating based on photoresist and nanocarbon, which comprises the following steps: using a nanocarbon material and photoresist as raw materials; the light absorption coating with low fluorescent background, low reflectivity and deep ultraviolet light aging resistance under the deep ultraviolet condition is obtained through the steps of preparing mixed turbid liquid, coating, pre-baking, removing photoresist through optical etching, removing residual photoresist, post-baking to form more holes and the like. When the low-background light absorption coating is applied to a deep ultraviolet fluorescence detector, compared with a traditional coating material of an organic adhesive and nanocarbon, the low-background light absorption coating provided by the invention has the advantages that the baseline of the detector is reduced by 67%, the baseline drift is reduced by 75%, and the baseline change degree under deep ultraviolet long-term aging is lower than 0.5%; the material shows excellent deep ultraviolet light aging resistance and extremely low fluorescence background, and can be applied to space telescopes, night vision devices, microscopes and various fluorescence detectors / sensors based on the characteristic of low reflectivity so as to obtain lower background and noise.

The invention discloses a fluorescence detector for analyzing ultracentrifugation. The fluorescence detector comprises an optical system, a metal panel and a vacuum stepping motor system, the optical system is fixed on the metal panel and is used for collecting a fluorescence signal generated by laser excitation of a sample in the ultracentrifugal sample pool through the first focusing lens; the vacuum stepping motor system comprises a sliding table, a radial stepping motor and an axial stepping motor, the metal panel is installed on the sliding table, the radial stepping motor drives the sliding table to move, and radial scanning is achieved; the axial stepping motor is connected with the first focusing lens and drives the first focusing lens to move up and down in the axial direction, and focusing adjustment is achieved. Through the design of an optical system, vacuum integration design and a vacuum stepping motor system, high-precision and stable fluorescence signal acquisition can be realized, and the device and the method can be widely applied to fluorescence detection for ultracentrifugation analysis.

Aspects of the present invention include methods for phase correcting signals from a light detection system (e.g., in a flow cytometer). Methods according to certain embodiments include detecting light from a sample having particles in a flow stream using a light detection system, the light detection system including a brightfield photodetector configured to generate a brightfield data signal and a fluorescence detector configured to generate a fluorescence data signal, and calculating a phase correction for the fluorescence detector based on the relative phase between the brightfield data signal and the fluorescence data signal. A system is also described having a processor having a memory operably coupled to the processor, the processor having instructions stored thereon that, when executed by the processor, cause the processor to calculate a phase correction for the fluorescence detector based on the brightfield data signal and the fluorescence data signal from the fluorescence detector. An integrated circuit device (e.g., a field programmable gate array) having programming for implementing the method is also provided.

The application provides a preparation method and application of a near-infrared two-region fluorescent sensor, and chiral single-walled carbon nanotubes are separated by using a double water phase extraction method; materials in the separation process include deionized water, polyethylene glycol, dextran, sodium dodecyl sulfate, sodium deoxycholate and cholic acid sodium hydrate; the chiral single-walled carbon nanotubes are dispersed and mixed with 4-nitrobenzene tetrafluoroboric acid diazonium salt to obtain the fluorescent sensor; high-purity (6,5)-SWCNT is efficiently and simply separated by using the double water phase extraction technology, the prepared SWCNT-NBD nanomaterial has good optical performance, including bright light emission in the near-infrared two-region, has good penetration capacity on biological tissues, the fluorescent detector (SWCNT-NBD) has low detection line, wide linear range, good selectivity on DA, and is convenient to prepare and low in cost.

The invention provides a fluorescence detector three-dimensional scanning method based on automatic sample injection and flow velocity control, and relates to the technical field of fluorescence detection.The fluorescence detector three-dimensional scanning method comprises the following steps that S1, first sample injection is conducted, and the flow velocity of a liquid chromatography pump is set to be constant F1; s2, the fluorescence detector recognizes the sample peak with the maximum response value of the first sample injection, the recognition time of the sample peak with the maximum response value is T1, and the wavelengths of the sample peak with the maximum response value are EX1 and EM1; s3, carrying out second sample introduction, and setting the flow rate of the pump to be constant F1; s4, monitoring the change of the response value of the fluorescence detector, and when the response value reaches the highest peak of the currently detected substance, setting the flow rate of the pump to be 0, so that the fluorescence detector performs detailed three-dimensional scanning; and S5, the data system automatically generates a 3D contour map or a three-dimensional curved surface map. The detection time is remarkably shortened through the automatic sample injection and rapid scanning functions, and meanwhile fluorescence characteristic scanning after mixture separation is achieved.

The invention discloses a novel one-pot RPA-CRISPR method for detecting monkey pox virus based on a sucrose-assisted multiphase solution system, sucrose is added in RPA reaction to increase the density of the RPA reaction, the RPA reaction is placed at the bottom of a tube, and the CRISPR reaction with lower density is kept in a water phase and located at the top of the RPA reaction; in a one-tube reaction process, newly accumulated RPA amplicons trigger non-specific cutting of Cas12a for ssDNA-FQ, so that a fluorophore is released, a real-time fluorescence signal is detected by using a fluorescence detector, and end point fluorescence can be visualized under a blue light source; compared with the prior art, the one-pot RPA-CRISPR method of the multiphase solution system is constructed by using the sucrose to increase the RPA reaction density, rapid, convenient and reliable detection of the monkey pox virus is realized, prevention and control of the monkey pox epidemic situation are facilitated, and a good example is provided for actual field detection application of the sucrose-assisted multiphase solution system.

Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.

This paper provides a method for detecting low concentrations of amphoteric compounds in a sample, comprising: (a) preparing an analytical sample mixture for capillary isoelectric focusing (CIEF) detection using the sample; and (b) detecting the analytical sample mixture using a fluorescence detector via a CIEF system; wherein the concentration of the low concentration amphoteric compound in the analytical sample mixture is less than 0.5 μg / μL. This method is more resistant to background noise interference and can sensitively detect and accurately quantify low concentrations of amphoteric compounds present in the sample.

The invention discloses a fluorescent algae analysis system and method, electronic equipment and a storage medium. The fluorescent algae analysis system comprises a light source driving circuit, a turbidity light source, a plurality of fluorescent light sources, a reference detector, a fluorescence detector, a turbidity detector and a data calculation module, the light source driving circuit is respectively connected with the turbidity light source and each fluorescent light source and is used for driving the turbidity light source and each fluorescent light source to work; the reference detector is used for detecting light intensity data of the turbidity light source or setting the fluorescence light source; the turbidity detector is used for detecting the turbidity of a water body to be detected; the fluorescence detector is used for detecting data of excited fluorescence with different intensities; the data calculation module is used for calculating the turbidity data and the fluorescence data to obtain algae analysis data. According to the fluorescent algae analysis system and method, the electronic equipment and the storage medium provided by the invention, the data stability and accuracy can be improved, and the influence of turbidity on a measurement result is eliminated.

The invention discloses a fluorescent probe for detecting arginine aminopeptidase as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of biological medicines. The fluorescent probe (DDAR) provided by the invention can be hydrolyzed by arginine aminopeptidase (RAP) at high selectivity to generate a hydrolysate of which the fluorescence attribute is remarkably changed, so that high-specificity detection can be realized through a fluorescence detector. Moreover, the probe reaction system can be used for quantitatively evaluating the activity and function of the arginine aminopeptidase in an intestinal microorganism sample, and can be used for screening an arginine aminopeptidase inhibitor. The DDAR provided by the invention has long fluorescence emission wavelength, can well weaken interference of biological background fluorescence, and has high sensitivity. The fluorescent probe for detecting arginine aminopeptidase provided by the invention can be prepared through a simple chemical synthesis process, the preparation process is simple and easy to implement, the detection cost of a fluorescent method is low, and the fluorescent probe has a very good application value.

The invention relates to an optical flow cytometer for fluorescence and scatter measurements, comprising: - a non-coherent light source intended to generate an illumination beam; - a beam truncation device comprising at least a first passage allowing a first portion of the illumination beam having a first divergence to pass, the first portion being dedicated to scatter measurements, and a second passage allowing a second portion of the illumination beam having a second divergence to pass, the second portion being dedicated to fluorescence measurements, the second divergence being greater than the first divergence, the first passage and the second passage being separated by a region of the beam truncation device blocking the illumination beam; - at least one focusing lens for focusing the first portion and the second portion of the illumination beam to a flow cell comprising an optical interrogation zone; - a flow cell intended to contain particles flowing through the optical interrogation zone; - a scatter detector for receiving light scattered from the first portion of the illumination beam when a particle passes through the optical interrogation zone; - a fluorescence detector for receiving fluorescence emitted by a particle passing through the optical interrogation zone.

The invention belongs to the technical field of organic compound analysis and detection, and particularly discloses an N-isobutyl-4-chloropyrrolidone analysis method which comprises the following steps: pretreating a sample to be analyzed; separating the to-be-derived sample liquid A by adopting two-dimensional chromatography; carrying out derivatization on the sample solution B to be derivatized; carrying out combined detection on the derivatization sample liquid through a fluorescence detector and a high-resolution mass spectrum; the N-isobutyl-4-chloropyrrolidone and the degraded impurities of the N-isobutyl-4-chloropyrrolidone are automatically identified and analyzed through CNN-LSTM double models. According to the analysis method of the N-isobutyl-4-chloropyrrolidone, the N-isobutyl-4-chloropyrrolidone containing chlorine and secondary amine groups is subjected to high-sensitivity and high-selectivity analysis in a complex matrix, so that the sensitivity and the selectivity of the N-isobutyl-4-chloropyrrolidone are improved, and the sensitivity and the selectivity of the N-isobutyl-4-chloropyrrolidone are improved. The method can be widely applied to medical intermediate quality control, organic synthesis process monitoring and functional material purity detection scenes.

The utility model provides an adjusting device for a microbiological detection light path, which comprises a laser, a flow chamber, a scattered light detector and a fluorescence detector, and a light spot shaping system, a scattered light collecting system and a fluorescence capturing system are respectively arranged between the flow chamber and the laser, between the flow chamber and the scattered light detector and between the flow chamber and the fluorescence detector. The flow chamber is arranged in the central block, the laser is arranged on the laser mounting seat, a first adjusting structure capable of adjusting the position of the light spot shaping system is arranged between the central block and the laser mounting seat, and a second adjusting structure capable of adjusting the positions of the scattered light detector and the scattered light collecting system is arranged between the scattered light detector and the central block. And a third adjusting structure capable of adjusting the positions of the fluorescence detector and the fluorescence capturing system is arranged between the fluorescence detector and the central block. The optical path structure is high in concentricity, convenient to adjust and high in overall optical path performance, and the optical path structure can be adjusted according to needs.

The application provides a flow analysis system signal processing method and device, equipment and a storage medium, and the method comprises the following steps: acquiring a detection signal of scattered light emitted by a detected particle under laser irradiation by a scattered light detector, to obtain an electric signal; analyzing the electric signal, excluding interference through a preset threshold, to obtain a starting time and an ending time of the detected particle passing through a detection area; acquiring a detection signal of fluorescence emitted by the detected particle under laser irradiation by a fluorescence detector of each fluorescence channel, to obtain a light pulse signal; recording generation conditions of the light pulse signal in the starting and ending intervals and analyzing to obtain a peak value and an area of the channel as a detection result of the channel signal. For the detection result of the photon counting detector, the method provided by the application can simply and quickly extract the area and height parameters of the event signal, can be compared with the detection and processing result of the traditional flow cytometer using an analog detector, and the method has strong compatibility.

A lamp lighting device is used to light a discharge lamp provided as a light source in a fluorescence detector. The discharge lamp has a configuration in which an anode and a cathode are arranged to be opposite to each other in a discharge container. The lamp lighting device includes a lamp driver, a lamp-voltage detector and a deterioration determiner. The lamp driver drives the discharge lamp. The lamp-voltage detector detects a lamp voltage in a monitoring period until a discharge state reaches a predetermined stable state after a discharge lamp in an unlit state is lit due to electrical breakdown. The deterioration determiner determines a deterioration state of the discharge lamp based on a detected lamp voltage.

The invention provides a flow analysis system signal processing method and device, equipment and a storage medium, and the method comprises the following steps: obtaining a detection signal of scattered light emitted by a scattered light detector to detected particles under laser irradiation, and obtaining an electric signal; analyzing the electric signal, eliminating interference through a preset threshold value, and obtaining starting time and ending time of the detected particles passing through the detection area; acquiring a detection signal of the fluorescence detector of each fluorescence channel on the fluorescence emitted by the detected particles under the irradiation of the laser to obtain a light pulse signal; and recording the generation condition of the optical pulse signal in the starting and ending intervals, and analyzing to obtain the peak value and the area of the channel as the detection result of the channel signal. Aiming at the detection result of the photon counting detector, the method provided by the invention can simply and quickly extract the area and height parameters of the event signal, and can be similar to the detection processing result of a simulation detector adopted by a traditional flow cytometer, so that the method has relatively strong compatibility.

Methods and devices for continuous flow monitoring of a liquid sample for presence of an airborne microbial pathogen are provided. The liquid sample can be derived from environmental air. The methods and devices provide for continuous labeling of a targeted pathogen in the liquid sample with a fluorescent probe. A customized fluorescence detector is provided that can detect labeled pathogens during continuous flow.

The invention relates to rock core oiliness evaluation equipment, an oiliness evaluation method and a storage medium, and relates to the technical field of oil exploration. The rock core oiliness evaluation equipment comprises a frame body; the conveying platform is installed on the frame body, and the conveying platform can drive the rock core sample borne above the conveying platform to move in the detection direction; a positioner; the detection device comprises a detection assembly arranged towards the preset scanning area, and the detection assembly comprises a laser emitter and a fluorescence detector; and the analysis system comprises a fluorescence spectrometer connected with the fluorescence detector. The core sample can be induced to generate fluorescence through laser emitted by the laser emitter, the intensity of the fluorescence is improved on the premise that the core is not damaged, the fluorescence generated by each section of the core sample can be continuously obtained by the fluorescence detector, and a continuous fluorescence spectrum can be generated through the fluorescence spectrometer connected with the fluorescence detector. Therefore, the oiliness analysis of each section of the rock core sample is realized.

The invention belongs to the technical field of analysis and detection, and particularly discloses a capillary electrophoresis fluorescence immunoassay method for detecting abrus precatorius toxin. A fluorescein isothiocyanate (FITC) labeled abrus precatorius toxin antibody probe is prepared, a non-competitive mode is adopted, a sample solution and an FITC labeled antibody are subjected to an incubation reaction, and the FITC labeled antibody is relatively excessive. The FITC labeled antibody-antigen compound and the excessive FITC labeled antibody in the mixed solution are divided into different zones in the separation capillary tube according to different migration rates, and fluorescence signal detection is performed through a window of a laser-induced fluorescence detector in sequence. Qualitative and quantitative analysis is carried out on the abrus precatorius toxin through the fluorescence signal peak and the intensity of the compound. According to the present invention, the detection method of the abrus precatorius toxin fluorescence immunocapillary electrophoresis is clarified, the detection automation is improved while the detection time is reduced compared with the traditional immunization method, the linear range of the abrus precatorius toxin standard solution detection is 75.8-606.4 nM, and the detection limit is 75.8 nM.

The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.

The invention discloses a food microorganism rapid detection device for food detection, relates to the technical field of food detection, and comprises a fluorescence detector, one side of which is fixedly connected with a fluorescence detection cabin. The food microorganism rapid detection device for food detection is provided with a test column, a swab and a protective film cover. When the test column and the swab are pulled out from the inside of a test tube, the swab can be moved to the vicinity of the food, and then the liquid bag part can be pinched to disconnect the connection between the breakable part and the limit block, thereby releasing the limit on the protective film cover, so that the protective film cover falls off from the outside of the swab, thereby effectively preventing the swab from being moved to the vicinity of the food when the swab is used, causing the swab to contact with the surface of other objects due to the user's carelessness, causing the swab to be contaminated, and then using the swab to collect food samples for food microorganism detection, which affects the accuracy of the detection result, affecting the accuracy of the food microorganism detection result.

The invention relates to the technical field of plant and crop detection and analysis, in particular to a non-contact chlorophyll detection method and system with distance adaptive compensation, a shell is of a cuboid structure, a display window and keys are arranged on the front side, a detection module is mounted at the front end, and a USB charging port is formed in the rear end. The detection module comprises a fluorescence detector (430 nm LED light emission and fluorescence signal reception), a distance sensor (distance measurement) and a temperature and humidity sensor (environment data acquisition). The microcontroller controls signal collection and calls a compensation model to calculate the chlorophyll concentration, the result is displayed on a window, and data can be transmitted through a USB. The system realizes non-contact automatic detection, avoids manual errors, improves precision, adapts to the environment and is convenient to operate. Therefore, the problems that existing chlorophyll detection is low in accuracy and online measurement cannot be achieved are solved.

The invention relates to a sensor for molecular interaction dynamics detection, which comprises an optical waveguide needle made of a high light guide material; one end of the optical waveguide needle is a connection end, and the other end is a detection end; the connecting end is connected with the laser and the fluorescence detector through optical fibers, and the surface of the detection end is coated with a rare earth up-conversion nano material; an enhanced modification area is arranged on the surface of the outer side wall, close to the detection end, of the optical waveguide needle, and the enhanced modification area is coated with a rare earth up-conversion nano material or plated with a reflecting film for enhancing light reflection in the optical waveguide needle; the detection end is used for being immersed into a solution to be detected to detect the interaction between target molecules and ligand molecules in the solution to be detected. The detected signal is the emission light intensity which is different from the detection signal of SPR and BLI in the prior art, and the technical problems that an existing SPR sensor is expensive, the detection result is easily interfered by impurities or particles, irradiation light and reflected light are easily interfered in the existing BLI detection process, the detection sensitivity and the signal-to-noise ratio are low and the like are solved.

The present invention discloses a fluorescent probe for detecting methionine aminopeptidase 1, and its preparation method and application. The fluorescent probe structural formula is shown in formula (1). The present invention also discloses its preparation method and application, and discloses a method for quantitatively detecting the activity of methionine aminopeptidase 1 using the fluorescent probe. The specific fluorescent probe provided by the present invention has high specificity during application and can be detected by a fluorescence detector. At the same time, the DDAN-MT provided by the present invention has a long-wavelength fluorescence emission wavelength, which can better reduce the interference of biological background fluorescence and has high sensitivity. In addition, the DDAN-MT provided by the present invention can be obtained by simple chemical synthesis, the synthesis process is simple and easy, and the fluorescence method detection cost is low.

The invention relates to the technical field of fluorescence detection, and discloses a material for trinitrotoluene fluorescence detection and a portable device. The fluorescent carbon quantum dot solution is prepared from the following raw materials: an amine compound, persulfate, acid liquor and alkali liquor. The fluorescent carbon quantum dot solution provided by the invention takes an amine compound as a preparation raw material, a large number of electron-rich amino groups are distributed on the surface of a carbon nucleus due to doping of nitrogen, a large number of negative potential sites exist on the surface of the carbon nucleus, and the fluorescent carbon quantum dot solution can react with nitroaromatic explosives with positive potential to bring fluorescence change and realize detection of the explosives; according to the fluorescence detection film, the fluorescent carbon quantum dots in a liquid medium are loaded on a solid medium, so that the fluorescent carbon quantum dots have portability, and the fluorescence characteristics of the fluorescent carbon quantum dots are not influenced; the fluorescence detection device containing the fluorescence detection film can detect trinitrotoluene in a liquid phase mode and a solid phase mode, and is high in sensitivity, simple to operate, low in cost and good in portability.

The invention pertains to an advanced method and system for performing real-time polymerase chain reaction (qPCR) tests (6), utilizing a machine- learning algorithm (4) to enhance the speed and accuracy of pathogen detection in food matrices. During the qPCR process, fluorescence data (3) is collected in real-time and analyzed by a computer (7) equipped with software (5) implementing a machine-learning algorithm (4), potentially realized as a neural network. This algorithm, trained on historical fluorescence data (3), predicts the test outcome—positive, negative, or no-amp—after each cycle, providing immediate feedback. The system (6) adapts to environmental and sample variances, continuously improving prediction accuracy through feedback from previous predictions. The qPCR system (6) includes a fluorescence detector (2), a computer (7) for data analysis, and software (5) implementing the machine-learning model (4), offering remote access and analysis capabilities. This approach significantly reduces the time required for traditional qPCR analysis, enhancing workflow efficiency and sample throughput. The invention is particularly beneficial for rapid and reliable food pathogen detection, ensuring timely interventions in food safety and quality control.