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Porous frame material for storing, transporting and preparing biological products

A technology for porous frameworks and biological products, applied in the fields of immunology, material chemistry and molecular biology, can solve problems such as peptide fragmentation, and achieve the effects of mild conditions, simple operation and improved stability.

Inactive Publication Date: 2018-08-28
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3) Due to the characteristics of proteins, there are main chains or branched chains with high flexibility, and proteins with relatively high rigidity are prone to peptide fragment breakage

Method used

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  • Porous frame material for storing, transporting and preparing biological products
  • Porous frame material for storing, transporting and preparing biological products
  • Porous frame material for storing, transporting and preparing biological products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of MOF-1 / human IgG complex:

[0029] Mix 50uL of PBS solution containing 10mg / mL Human IgG with 200μL of 200mM 2-imidazole aqueous solution, mix well, and then add 40mM 250μL zinc ion aqueous solution to the protein-imidazole aqueous solution. The system was placed at 4°C for 10 minutes. After the reaction, the reaction mixture was centrifuged at 6000rmp, and the supernatant was discarded to obtain a white solid, which was pre-frozen at -80°C and then lyophilized for 8 hours. The powder X-ray diffraction pattern and Fourier transform infrared transform spectrum of the MOF-1 / human IgG complex obtained from the reaction prove that the MOF-1 material is obtained, and the human IgG antibody has been wrapped into the MOF-1.

[0030] Preparation of MOF-1 / GOx complex:

[0031] Dissolve 5mg of GOx (glucose oxidase) in 2.5mL 160Mm imidazole solution, mix well, and then add 40mM 2.5mL zinc ion aqueous solution to the GOx-imidazole aqueous solution. The system was p...

Embodiment 2

[0035] The experiment of recovering the encapsulated IgG antibody and GOx enzyme from the MOF-1 / goat anti-BSA IgG complex and MOF-1 / GOx complex: the recovered lyophilized MOF-1 / goat anti-BSA IgG complex and Add the MOF-1 / GOx complex to the same 20mM EDTA solution as its reaction system and mix. After 15 minutes of reaction, use a palm centrifuge to briefly centrifuge for 5 seconds, and use an ultrafiltration tube to ultrafilter at 10,000g for 10-20 minutes (the specific time needs to be Consider the concentration and molecular weight of the protein biological product and the pore size of the ultrafiltration tube membrane used). After the ultrafiltration, the ultrafiltration membrane was washed with 1×PBS to recover the antibody. Use the A280 method to detect the concentration of the recovered antibody, and obtain the recovery data. After calculation, compare with the total amount of antibody added originally. The final recovery rate is not less than 80%. Recovery rate = the...

Embodiment 3

[0037] Antibody protective testing. Goat anti-BSA IgG solution (0.5mg / ml) and goat anti-BSA IgG / MOF-1 complex produced aggregates and degraded through three different accelerated stress methods. Heat stress was performed by incubating at 75°C for 20 min in 1.5 ml reaction tubes. Catalyzed oxidation of metal: mix the antibody sample to be tested with 8mM ascorbic acid and 0.08mM CuCl 2 Reaction at room temperature for 3h. Mechanical force: Extrude the goat anti-BSA IgG / MOF-1 complex with a pressure of 20MPa.

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Abstract

In order to solve the problems that some biological products are poor in stability, cannot be stored at normal temperature, cannot be well stored easily and are not convenient to use in emergency circumstances such as a disaster, the invention relates to a porous frame material creatively applied to biomolecule storage, and the stability and other performances are improved. The porous frame material and biomolecules are used for forming a porous frame material / biomolecule compound, the biomolecule is wrapped, and therefore the protection effect is achieved. On the premise of keeping the biomolecule activity, the system can achieve efficient separation and recycling of porous materials and biomolecules. While a biological product protecting system is developed, various performances are comprehensively detected and evaluated, the technical purposes of synthesis, storage, release and the like are solved, the good technical effect is achieved, the biomolecules can be effectively protected,and the material is used for storing a biological agent and preparing novel preparations.

Description

technical field [0001] The present invention relates to material chemistry, molecular biology and immunology, especially the application of porous framework materials in storage, transportation and delivery of biological products. Background technique [0002] With the continuous development of biotechnology, biological products have played an increasingly important role in the diagnosis and treatment of diseases. However, some biological products such as vaccines, antibodies, conventional proteins, and enzymes are easily affected by external factors and lose their activity. For example, vaccines, antibodies, and protein drugs are very sensitive to temperature and require stable storage and transportation conditions at low temperatures. In order to ensure low temperature, a lot of professional equipment must be equipped in the storage and transportation, and a lot of manpower and material resources are consumed, which also limits the application range and ways of these biolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/00C12N9/96
CPCC12N9/96C07K1/00C12N15/86C12N2710/10343A61K38/14C07K16/18C07K2317/92A61K38/43A61K38/443C12Y101/03004A61K35/761A61K39/3955A61K47/02A61K47/24
Inventor 陈瑶张振杰冯一帆
Owner NANKAI UNIV
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