30 results about "Biomolecular complex" patented technology

The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.

The invention provides a method for identifying a key module and a key node by fusing multiple methods based on a topological structure of a biomolecular complex network such as a protein interaction network, a gene expression regulation network, a biological metabolism network, an epigenetic network, a phenotype network or a signal conduction network. The method comprises the following main steps of performing module division on the network based on the biomolecular network, and comprehensively and quantitatively identifying the key module and the key node from multiple angles by adopting multiple measurement methods based on the topological structure of the network for the network or each module.

In order to solve the problems that some biological products are poor in stability, cannot be stored at normal temperature, cannot be well stored easily and are not convenient to use in emergency circumstances such as a disaster, the invention relates to a porous frame material creatively applied to biomolecule storage, and the stability and other performances are improved. The porous frame material and biomolecules are used for forming a porous frame material / biomolecule compound, the biomolecule is wrapped, and therefore the protection effect is achieved. On the premise of keeping the biomolecule activity, the system can achieve efficient separation and recycling of porous materials and biomolecules. While a biological product protecting system is developed, various performances are comprehensively detected and evaluated, the technical purposes of synthesis, storage, release and the like are solved, the good technical effect is achieved, the biomolecules can be effectively protected,and the material is used for storing a biological agent and preparing novel preparations.

The present application pertains to improved methods of detecting biomolecules in a biological sample (or system), In particular, embodiments discussed herein allow for the detection of biomolecule complexes. The embodiments enable for the first time the elucidation of the significance of biomolecule complexes for certain disease states, which in turn enables the diagnosis of disease states based on the identity and complexing level of a biomolecule complex in a particular biological sample.

A method for identifying a key module and a key node of a biomolecular complex network by fusing multiple methods on the basis of a topological structure of the biomolecular complex network which may be such as a protein-protein interaction network, a gene expression regulation network, a biological metabolism network, an epigenetic network, a phenotypic network or a signaling network, comprising the following main steps: according to the biomolecular network and a module division for the network, comprehensively and quantitatively identifying the key module and key node on the basis of the topological structure by various measuring methods from multi-angle.

The invention relates to a photothermal preparation based on biliverdin, comprising biliverdin, biliverdin gel, biliverdin- Metal chelates, biliverdin-Biomolecular complex, biliverdin-Biomolecule-Metal functional assembly. The photothermal preparation has the following advantages: 1) near-infrared absorption and good tissue penetration depth, The preparation of this series of biliverdin-based photothermal preparations is of great significance for the development of multifunctional phototherapeutic drugs, and has great potential applications in tumor, dermatosis (such as pigment diseases), vascular diseases and so on. 3) Biocompatibility and metabolic mechanism are clear.

Provided herein is a one-step method for chelating actinium-225 to a construct comprising a chelator linked to a bio-molecule, such as, an antibody or monoclonal antibody, via a bifunctional ligand in, for example, a 3-arm configuration. Also provided are methods for increasing the radiochemical yield of an actinium-225-chelant-biomolecule complex and for producing a high specific activity actinium-225 complex. The chelation is performed at a physiological temperature, about 37° C. Also provided are high specific activity actinium-225 complexes, that is, actinium-225 chelated to the chelator-biomolecule construct and pharmaceutical compositions thereof. Further provided are methods of treating a neoplastic disease or disorder with the actinium-225 complexes.

The present application pertains to improved methods of detecting biomolecules in a biological sample (or system), In particular, embodiments discussed herein allow for the detection of biomolecule complexes. The embodiments enable for the first time the elucidation of the significance of biomolecule complexes for certain disease states, which in turn enables the diagnosis of disease states based on the identity and complexing level of a biomolecule complex in a particular biological sample.

The invention relates to compositions of DOTA derivative compounds, lanthanoid-DOTA derivative molecular complex, and lanthanoid-complex encapsulated solid lipid particles or capsules, and methods of making and using the compositions. The solid lipid particles or capsules contain micelle cores stabilized by a hyperbranched polymer shell based from a crosslinked DOTA derivative compound or crosslinked lanthanoid-DOTA derivative complex. These solid lipid particles or capsules can be used in various applications, such as contrast agents or drug delivery vehicles.

The invention provides methods of dynamically simulating molecular interactions between a target molecule and a plurality of ligand molecules. The ligand molecules may be presented in the model as a homogeneous set of identical ligands or as a heterogeneous set of different ligands, such as, for example, a set of structural variants of a ligand molecule. Typically, the ligand molecule will be a small organic compound, such as a drug or other small molecule, and the ligand will be a protein or a protein domain, a nucleic acid (i.e., DNA, RNA), or a biomolecular complex of proteins and / or nucleic acid molecules. Unlike all known molecular dynamics simulation methods, the invention provides ligand molecules to the simulation's interaction environment(s) in excess relative to the target molecule.

A novel composite biological agent based on a porous frame material, comprising porous frame materials and biomolecules. The porous frame materials cover a biological product, wherein the porous frame materials are metal-organic frame material (MOFs), covalent organic frame materials (COFs), and hydrogen-bonding organic frame materials (HOFs), and the biomolecules are any one or a combination of antibodies, enzymes, peptides, vaccines, nucleotides, and virus species. The composite biological agent uses the porous frame materials and biomolecules to form a porous frame material / biomolecule complex, and the biomolecules are coated to achieve the protection effect. Under the premise of remaining biomolecule activity, the system can achieve efficient separation and recovery of the porous materials and the biomolecules, so that the technical problems of synthesis, storage, release, etc. are solved, a good technical effect is achieved, and the biomolecules are effectively protected. The system is applied to the storage and transportation of biological agents and preparation of novel agents.

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

A method is described for obtaining antibodies binding specifically to an epitope resulting from in vivo modification of a biomolecule or biomolecular complex, where the in vivo modification results from expression of a transgene in an animal. An example of such a biomolecule or biomolecular complex is LDL and an example of such a transgene is human myeloperoxidase. Resulting antibodies and methods of using the antibodies are also described.

The present invention relates to complexes comprising one or more markers and one or more biomolecules for use as a contrast agent in positron emission tomography based imaging. The complexes according to the present invention preferably accumulate in a target compartment, such as the kidney. The present invention further relates to methods for generating the complexes and methods for using the complexes such as for evaluation of different aspects of kidney functionality e.g. for calculation of total and / or regional glomerular filtration rate in the kidney without the need for sampling blood or urine.

The present invention provides a biomolecular composite which is a ghost cell which has been emptied of all or substantially all of the cytosolic contents of the cell including the nucleus and nuclear contents, preferably further having been fused with exogenous amphipathic molecules, wherein said composite has a hydrodynamic diameter of less than 6 μm. The biomolecular composite may contain a cargo molecule and be capable of targeting a cell or tissue. Also provided are methods of preparing ghost cells, methods of preparing biomolecular composites and methods for their use.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention discloses a nanocapsule-nucleic acid biomolecule compound capable of simultaneously detecting Hg2+ and Ag+ and based on an exonuclease cyclic amplification technology and a preparation method of the nanocapsule-nucleic acid biomolecule compound. The Hg2+ and the Ag+ can be simultaneously detected by adopting the nanocapsule-nucleic acid biomolecule compound provided by the invention, and on the premise of ensuring high specificity, the detection sensitivity is obviously improved; and meanwhile, a wider linear detection range for Hg2+ and Ag+ is further obtained.

A method includes enriching biomolecules and removal of the biomolecules from a biological sample. In the presence of particles, an alginate solution and salts of divalent and / or polyvalent cations or an acid are added to a biological sample, and an alginate-gel-biomolecule-complex is formed on the particles. The complex is removed from the sample by separation of the particles, and from which subsequently the biomolecules or ingredients of the biomolecules are released. The biomolecules, which shall be enriched, include cell-free nucleic acids, viruses or subcellular microparticles.

The present invention relates to a photothermal preparation based on biliverdin, including: biliverdin, biliverdin gel, biliverdin-metal chelate, biliverdin-biomolecular complex, biliverdin-biomolecule-metal functional assembly. The prepared photothermal preparation has the following advantages: 1) near-infrared absorption and good tissue penetration depth; 2) high photothermal conversion efficiency; 3) biocompatibility and clear metabolic mechanism. The preparation of this series of biliverdin-based photothermal preparations is of great significance for the development of multifunctional phototherapeutic drugs, and has great application potential in tumors, skin diseases (such as pigmentary diseases), and vascular diseases.

Environmental and water pollution caused by high-toxicity heavy metals is always a serious problem faced by human beings. As < 3 + >, Pb < 2 + > and Hg < 2 + > are environmental pollutants which have serious toxicity and are widely distributed, so that the environment and human beings can be seriously threatened even at low concentration. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting As < 3 + >, Pb < 2 + > and Hg < 2 + > is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method capable of simultaneously detecting As < 3 + >, Pb < 2 + > and Hg < 2 + >, the method adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, so that the As < 3 + >, Pb < 2 + > and Hg < 2 + > can be simultaneously detected, the detection sensitivity is remarkably improved on the premise of ensuring high specificity, and meanwhile, a wider linear detection range is obtained.

Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and / or at least one molecular weight standard.

The subject matter of the invention is a method for conjugation of free thiol group(s) containing biomolecules, leading to the biomolecular complex formation, comprising a reaction to connect biomolecules using a gold-donor agent in which a —S—Au—S— bond is formed, characterised in that a gold-donor agent is halogen(triarylphosphine)gold (I). The subject matter of the invention is also the use of halogen(triarylphosphine)gold (I) molecules as the gold-donor agent in the method of biomolecular complex formation.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method for simultaneously detecting Hg2+ and Ag+ based on single excitation, the method utilizes a single excitation technology and adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, not only can realize simultaneous detection of Hg2+ and Ag+, but also obviously improves the detection sensitivity on the premise of ensuring high specificity, and meanwhile, obtains a wider linear detection range on Hg2+ and Ag+.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method capable of simultaneously detecting Hg2+ and Ag+. The method adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, the simultaneous detection of Hg2+ and Ag+ can be realized, the detection sensitivity is obviously improved on the premise of ensuring high specificity, and meanwhile, the detection sensitivity is greatly improved; and meanwhile, a wider linear detection range for Hg2+ and Ag+ is further obtained.

Environmental and water pollution caused by high-toxicity heavy metals is always a serious problem faced by human beings. As < 3 + >, Pb < 2 + > and Hg < 2 + > are environmental pollutants which have serious toxicity and are widely distributed, and can seriously threaten the environment and human health even at low concentration. The invention provides a method for simultaneous fluorescence imaging of As < 3 + >, Pb < 2 + > and Hg < 2 + > in cells by using a nanocapsule-nucleic acid biomolecule compound. The provided and constructed nanocapsule-nucleic acid biomolecule compound has the advantages of being simple in structure, good in biocompatibility, ingenious in design, easy to prepare, stable in performance, high in cell membrane infiltration capacity, high in intracellular release controllability, high in specificity, short in response time and the like and high-sensitivity and high-selectivity simultaneous fluorescence imaging of As < 3 + >, Pb < 2 + > and Hg < 2 + > in cells can be conveniently and quickly realized.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The invention provides a simultaneous fluorescence imaging method for Hg2+ and Ag+ in cells. The simultaneous fluorescence imaging method can sensitively and efficiently realize simultaneous fluorescence imaging for Hg2+ and Ag+ in cells by adopting a nanocapsule-nucleic acid biomolecule compound. The nanocapsule-nucleic acid biomolecule compound provided and constructed by the invention has the advantages of being simple in structure, good in biocompatibility, ingenious in design, simple to prepare, stable in performance, high in cell membrane infiltration capacity, high in intracellular release controllability, high in specificity to Hg2+ and Ag+, short in response time, convenient to monitor in real time and the like, and can realize high-sensitivity and high-selectivity simultaneous fluorescence imaging of Hg2+ and Ag+ in cells conveniently and quickly.

The invention discloses a method of conjugating a substrate comprising exchanging a counterion bound to a biomolecule with a lipophilic counterion to form a biomolecular complex, dispersing the biomolecular complex in a non-aqueous solvent, and The biomolecular complex is coupled to the substrate in the presence of an aqueous solvent. The substrate or solid support is a polymer particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counterion is tetrabutylammonium ion, and the non-aqueous solvent is N-methylpyrrolidone (NMP). Oligonucleotide-coated particles are used in nucleic acid isolation or nucleic acid sequencing reactions.