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Nanocapsule-nucleic acid biomolecule compound capable of simultaneously detecting Hg2+and Ag+ and preparation method of nanocapsule-nucleic acid biomolecule compound

A nanocomposite and biomolecule technology, applied in the field of environmental monitoring and nanobiology, to achieve the effect of excellent performance, easy synthesis and wide application range

Pending Publication Date: 2021-08-13
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, nanocapsule-nucleic acid biomolecular complexes have been used to simultaneously detect Hg based on enzymatic cycle amplification technology, using hollow porous nanomaterials, and utilizing the biorecognition effect of base mismatches on two metal ions. 2+ and Ag + technology has not yet been reported in the literature

Method used

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  • Nanocapsule-nucleic acid biomolecule compound capable of simultaneously detecting Hg2+and Ag+ and preparation method of nanocapsule-nucleic acid biomolecule compound

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Embodiment 1

[0028] A preparation method proposed by the present invention that can simultaneously detect Hg 2+ and Ag + The method for the nanocapsule-nucleic acid biomolecule complex based on exonuclease cycle amplification technology and biomolecular recognition technology comprises the following steps:

[0029] (1) By selecting different types and numbers of bases, two kinds of nucleic acid biorecognition molecules were designed and synthesized to ensure that they are respectively and only for the target metal ion Hg 2+ or Ag + Have a specific response, but no response to any other coexisting ions and interference ions;

[0030] (2) Wash 20 μL magnetic beads with MOPS buffer solution with pH=7.0, add 400 μL nano-gold carrier solution with hollow and porous structure, add 200 μL 11.664 mg / mL polydiallyldimethylammonium chloride solution, 37 After 10 hours at ℃, magnetically separate and wash with MOPS buffer solution with pH=7.0;

[0031] (3) Add 2 μL of rhodamine B solution (final ...

Embodiment 2

[0038] A method that can detect Hg at the same time proposed by the present invention 2+ and Ag + Hg 2+ and Ag + The simultaneous detection method is as follows:

[0039] (1) 20 μL containing Hg 2+ and Ag + The solution added to the present invention can detect Hg simultaneously 2+ and Ag + In the nanocapsule-nucleic acid biomolecule complex based on exonuclease cycle amplification technology and biomolecular recognition technology, dilute to 200 μL with MOPS buffer solution with pH=7.0, mix well, add 1 μL EXOⅢ shearing enzyme ( 20U), put it into a shaker for 2h, the reaction temperature is 37°C, due to the Hg 2+ and Ag + In the presence of the nucleic acid biomolecules assembled on the surface of the nanocapsules, the Hg 2+ and Ag + A specific response occurs, and the two ions are specifically combined with their corresponding nucleic acid biomolecules, causing their conformation to change, falling off the surface of the nanocapsule, and opening the blocked orifice,...

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Abstract

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention discloses a nanocapsule-nucleic acid biomolecule compound capable of simultaneously detecting Hg2+ and Ag+ and based on an exonuclease cyclic amplification technology and a preparation method of the nanocapsule-nucleic acid biomolecule compound. The Hg2+ and the Ag+ can be simultaneously detected by adopting the nanocapsule-nucleic acid biomolecule compound provided by the invention, and on the premise of ensuring high specificity, the detection sensitivity is obviously improved; and meanwhile, a wider linear detection range for Hg2+ and Ag+ is further obtained.

Description

technical field [0001] The present invention relates to Hg 2+ and Ag + Simultaneous detection, specifically related to a simultaneous detection of Hg 2+ and Ag + The nanocapsule-nucleic acid biomolecule compound and its preparation method based on exonuclease cycle amplification technology and biomolecular recognition technology belong to the field of environmental monitoring and nanobiotechnology. Background technique [0002] Mercury ion (Hg 2+ ) and silver ions (Ag + ) are two metal ions with severe toxicity and are also widely distributed environmental pollutants, which pose serious threats to the environment and human beings even at low concentrations, causing severe and permanent damage. More seriously, through polluted water bodies, Hg 2+ and Ag + It can continuously accumulate in agricultural and aquatic products and enter the human food chain. If prolonged exposure to Hg 2+ and Ag + In a polluted environment, it will lead to the slow occurrence of degenera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/53
CPCG01N21/6486G01N33/5308
Inventor 王卫唐凯董传诚李欣宋志灵罗细亮
Owner QINGDAO UNIV OF SCI & TECH
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