Method for simultaneously detecting As < 3 + >, Pb < 2 + > and Hg < 2 + >

Abstract

Environmental and water pollution caused by high-toxicity heavy metals is always a serious problem faced by human beings. As < 3 + >, Pb < 2 + > and Hg < 2 + > are environmental pollutants which have serious toxicity and are widely distributed, so that the environment and human beings can be seriously threatened even at low concentration. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting As < 3 + >, Pb < 2 + > and Hg < 2 + > is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method capable of simultaneously detecting As < 3 + >, Pb < 2 + > and Hg < 2 + >, the method adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, so that the As < 3 + >, Pb < 2 + > and Hg < 2 + > can be simultaneously detected, the detection sensitivity is remarkably improved on the premise of ensuring high specificity, and meanwhile, a wider linear detection range is obtained.

Description

technical field

[0001] The present invention relates to As 3+ , Pb 2+ , Hg 2+ The simultaneous detection of, specifically relates to a simultaneous detection of As 3+ , Pb 2+ and Hg 2+ The nanocapsule-nucleic acid biomolecule complex based on exonuclease cycle amplification technology and biomolecular recognition technology and its preparation method and application belong to the field of environmental monitoring and nanobiotechnology. Background technique

[0002] Environmental and water pollution caused by highly toxic heavy metals has always been a major concern of people, and they can enter the food chain through air, water and soil and seriously endanger the ecosystem.

[0003] Arsenic is an element that exists widely in nature and has metal-like properties. Arsenic and its compounds have been recognized as carcinogens by many international authorities such as the International Agency for Research on Cancer under the World Health Organization (WHO) and the US Envi...

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