Automatic nucleic acid extraction detector and detection method thereof

Abstract

The invention provides an automatic nucleic acid extraction detector and a detection method thereof. The automatic nucleic acid extraction detector comprises a chip and a hardware system, wherein the chip comprises a supporting body and a plurality of containing cavities with elastic outer walls, the containing cavities are formed in the supporting body, the containing cavity comprises a sample pool, a cracking pool, a washing solution pool, an LAMP buffer solution pool, an amplification detection pool and a waste liquid pool, and the hardware system comprises a side extrusion device for inserting the chip, a temperature control module for adjusting a temperature of each containing cavity, a magnetic force control element for controlling a movement direction of magnetic beads, a fluorescence detection module for detecting nucleic acid and a microcontroller for regulating and controlling operation of the automatic nucleic acid extraction detector. The nucleic acid detection method comprises steps of chip insertion, lysis, washing, elution, amplification, detection and the like. According to the automatic nucleic acid extraction detector and the detection method, cross contamination during nucleic acid detection is avoided, a detection sample can be rapidly replaced, and automatic detection of nucleic acid is facilitated.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a nucleic acid extraction and detection method. Background technique [0002] Nucleic acid detection is a method to identify biological species, and an important means for the detection and identification of viruses, bacteria and other microorganisms, and has been widely used in the field of biomedical research. Nucleic acid detection steps generally include extracting nucleic acid from a sample, purifying nucleic acid, concentrating and then performing nucleic acid detection, etc. [0003] At present, the above steps are mainly completed by nucleic acid extractors and detection equipment. Due to the complexity of the operation steps of the above instruments and strong professionalism, they can only be handled by specific staff, which is time-consuming, laborious and inflexible. At the same time, most of the currently used nucleic acid extractors and testing equipment are ope...

AI Technical Summary

Problems solved by technology

[0003] At present, the above steps are mainly completed by nucleic acid extractors and detection equipment. Due to the complexity of the operation steps of the above instruments and strong professionalism, they can only be handled by specific staff, which is time-consuming, laborious and inflexible.

At the same time, most of the currently used nucleic acid extractors and testing equipment are open, which can easily cause cross-contamination of nucleic acids and affect the accuracy of test results.

When nucleic acid detection is performed on pathogenic bacteria or viruses, it is easy to affect the health of the staff and there are potential safety hazards

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