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Quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis and detection method of kit

A technology of time-resolved fluorescence and reagent kit, which is applied in the field of medical and health care, and can solve problems such as positive tuberculin test

Inactive Publication Date: 2016-10-26
GUANGZHOU FENGHUA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, BCG vaccination, infection with non-pathogenic mycobacteria, and other factors can lead to a positive tuberculin test in non-TB-infected individuals

Method used

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  • Quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis and detection method of kit
  • Quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis and detection method of kit
  • Quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis and detection method of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Dry Reagent Rapid Time-Resolved Fluorescence Immunoassay Kit for Tuberculosis Infected T Cell Detection

[0043] A rapid time-resolved fluorescent immunoassay kit for dry reagent detection of tuberculosis-infected T cells, the kit comprising:

[0044] 1) Test cuvette, which is coated with streptavidin, linked with biotinylated γ-interferon monoclonal antibody, and contains europium-labeled γ-interferon monoclonal antibody;

[0045] 2) calibrator cuvette, which is coated with streptavidin, connected with biotinylated γ-interferon monoclonal antibody, and contains europium-labeled γ-interferon monoclonal antibody and calibrator of different concentrations; calibrator Containing 0, 6, 300, 15000pg / mL gamma-interferon antigen;

[0046] 3) Blank control culture tube, which contains 15IU / mL blood heparin sodium, and the inside is in a vacuum negative pressure state;

[0047] 4) The test culture tube contains a combination of 15 IU / mL blood heparin sodium and 5 μg / mL Rv387...

Embodiment 2

[0085] Dry Reagent Rapid Time-Resolved Fluorescence Immunoassay Kit for Tuberculosis Infected T Cell Detection

[0086] A rapid time-resolved fluorescent immunoassay kit for dry reagent detection of tuberculosis-infected T cells, the kit comprising:

[0087] 1) Test cuvette, which is coated with streptavidin, biotinylated γ-interferon monoclonal antibody and europium-labeled γ-interferon monoclonal antibody;

[0088] 2) calibrator cuvette, same as embodiment 1;

[0089] 3) Blank control culture tube, which contains 9.8IU / mL blood heparin potassium, and the inside is in a vacuum state;

[0090] 4) The test culture tube contains heparin potassium at 9.8IU / mL blood and chimeric recombinant antibodies of Rv3874 (CFP-10) and Rv3875 (ESAT-6) at 1 μg / mL, and the inside is in a vacuum state;

[0091] 5) Positive control culture tube, which contains 9.8 IU / mL blood heparin potassium and 1 μg / mL concanavalin (ConA), and the inside is in a vacuum state;

[0092] 6) experimental buff...

Embodiment 3

[0115] Dry Reagent Rapid Time-Resolved Fluorescence Immunoassay Kit for Tuberculosis Infected T Cell Detection

[0116] A rapid time-resolved fluorescent immunoassay kit for dry reagent detection of tuberculosis-infected T cells, the kit comprising:

[0117] 1) Test cuvette, which is coated with streptavidin, biotinylated γ-interferon monoclonal antibody and europium-labeled γ-interferon monoclonal antibody;

[0118] 2) calibrator cuvette, same as embodiment 1;

[0119] 3) Blank control culture tube, which contains 28IU / mL blood heparin sodium, and the inside is in a vacuum state;

[0120] 4) The test culture tube contains 28 IU / mL blood heparin sodium and 10 μg / mL Rv0733, and the inside is in a vacuum state;

[0121] 5) Positive control culture tube, which contains 28 IU / mL blood heparin sodium and 20 μg / mL concanavalin (ConA), and the inside is in a vacuum state;

[0122] 6) experimental buffer, with embodiment 1;

[0123] 7) concentrated lotion, with embodiment 1.

...

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Abstract

The invention discloses a quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis. The kit is characterized by comprising 1) a test reaction cup; 2) a calibrator reaction cup; 3) a blank control culture tube containing an anticoagulant substance inside; 4) a test culture tube; 5) a positive control culture tube containing an anticoagulant substance and cell agglutinin inside; 6) an experiment buffer solution and 7) a wash concentrate. The invention further discloses a detection method of the quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis. The kit adopts a gamma-interferon in-vitro release method, a biotin-avidin method and double-antibody sandwich time-resolved fluorescence immunoassay together, the experimental methods are simple and quick, automatic operation is realized, and the detection system is an open type operation system.

Description

technical field [0001] The invention belongs to the medical and health field, and in particular relates to a fast time-resolved fluorescent immunoassay kit suitable for detecting tuberculosis-infected T cells and a detection method thereof. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB), Mycobacterium bovis and Mycobacterium africanum in the Mycobacterium tuberculosis complex, which can affect various organs of the body. Among them, pulmonary tuberculosis is more common. Mycobacterium tuberculosis is generally transmitted from respiratory tuberculosis patients to new hosts through air droplets. The incubation period for tuberculosis is several weeks to several months, during which most infected people are asymptomatic. Tuberculosis seriously endangers public safety, and 2 million people die of tuberculosis every year in the world. In China, there are 1 million new tuberculosis patients every year. [...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/569
CPCG01N33/505G01N33/5695G01N2333/35
Inventor 谭玉华范主桥岑柏坚
Owner GUANGZHOU FENGHUA BIOENG
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