SARS-CoV-2 inactivated vaccine and preparation method of vaccine
An inactivated vaccine, sars-cov-2 technology, applied in the field of SARS-CoV-2 inactivated vaccine and its preparation, to achieve the effects of reducing impurity content, simple operation, and reducing biosafety risks
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Embodiment 1
[0041]Embodiment 1, prepare SARS-CoV-2 virus inactivated vaccine example 1
[0042] (1) The virus seed adopts the new coronavirus strain 2019nCoVWIV04-1, and the cells used for production are Vero cells;
[0043] (2) Digest and disperse the resuscitated Vero cells with trypsin substitute TrypLE Select, add 199 culture medium containing 10% newborn bovine serum, mix evenly, add to the cell factory, and culture at 37°C until a uniform monolayer;
[0044] (3) After Vero cells were cultured in a 10-layer cell factory for 6 days, the cell density was 3×10 7 cells / ml, discard the cell culture medium and add 1.6L virus maintenance solution;
[0045] (4) The virus maintenance liquid that will contain working virus seed, working virus seed titer 8.00LgCCID 50 / ml, add 1.6L virus maintenance solution containing 0.01ml working virus seed to each cell factory, and the virus is cultured in the cell factory, the culture condition is 37℃±1℃;
[0046] (5) After the virus is cultured for 3 ...
Embodiment 2
[0052] Embodiment 2, prepare SARS-CoV-2 virus inactivated vaccine example 2
[0053] (1) The virus seed adopts the new coronavirus strain 2019nCoVWIV04-1, and the cells used for production are Vero cells;
[0054] (2) Digest and disperse the resuscitated Vero cells with trypsin substitute TrypLE Select, add 199 culture medium containing 10% newborn bovine serum, mix evenly, add to the cell factory, and culture at 37°C until a uniform monolayer of Vero cells is obtained. cell;
[0055] (3) After Vero cells were cultured in a 10-layer cell factory for 6 days, the cell density was 3×10 7 cells / ml, discard the cell culture medium and add 1.6L virus maintenance solution;
[0056] (4) Add the virus maintenance solution containing the working virus species into the cell factory, and the titer of the working virus species is 8.00LgCCID 50 / ml, add 1.6L virus maintenance solution containing 0.01ml working virus seed to each cell factory, and the virus is cultured in the cell factory...
Embodiment 3
[0063] Embodiment 3, prepare SARS-CoV-2 virus inactivated vaccine example 3
[0064] (1) The virus seed adopts the new coronavirus strain 2019nCoVWIV04-1, and the cells used for production are Vero cells;
[0065] (2) Digest and disperse the resuscitated Vero cells with trypsin substitute TrypLE Select, add 199 culture medium containing 10% newborn bovine serum, mix evenly, add to the cell factory, and culture at 37°C until a uniform monolayer of Vero cells is obtained. cell;
[0066] (3) After Vero cells were cultured in a 10-layer cell factory for 6 days, the cell density was 3×10 7 cells / ml, discard the cell culture medium and add 1.6L virus maintenance solution;
[0067] (4) Add the virus maintenance solution containing the working virus species into the cell factory, and the titer of the working virus species is 8.00LgCCID 50 / ml, add 1.6L virus maintenance solution containing 0.01ml working virus seed to each cell factory, and the virus is cultured in the cell factory...
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