Brassica napus Bna.A08IDD7 gene promoter and application thereof

Abstract

The invention discloses a brassica napus Bna.A08IDD7 gene promoter and application thereof, the nucleotide sequence of the promoter is shown as SEQ ID NO.5, and the promoter can be strongly expressedin roots, leaves, stems, flowers, siliques, seeds and other tissues of arabidopsis thaliana; therefore, it is indicated that the sequence has very strong expression activity and characteristics of a constitutive promoter, and can be used as a strong constitutive promoter to be applied to plant genetic engineering research. Therefore, the promoter creates excellent germplasm resources and other genetic engineering research aspects; the promoter has good application potential, can replace a cauliflower mosaic virus (CaMV) 35S promoter commonly used in current plant genetic engineering research,constructs a plant transgenic expression vector, is applied to plant genetic engineering, and can widely cultivate transgenic plants with high biosafety.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the Bna.A08IDD7 gene promoter of Brassica napus, and also relates to the application of the promoter. Background technique

[0002] my country is the world's largest producer of rapeseed (Brassica napus) and consumer of rapeseed oil. It can provide about 5.2 million tons of high-quality edible oil every year, accounting for 47% of domestic vegetable oil. However, at present, the supply situation of edible vegetable oil in my country is still relatively severe, and the self-production is insufficient, which seriously threatens the safety of edible vegetable oil in my country. Rapeseed production mainly utilizes fallow fields in winter, and does not compete with food crops for land. Currently, there are still 66hm of fallow fields in the Yangtze River Basin 2 All of the above have great potential for developing the rapeseed industry.

[0003] The plant gene promoter plays a key role ...

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