30 results about "Brucella antibody" patented technology

The invention discloses a Brucella antibody immune colloidal-gold detection test strip, wherein, staphylococcus aureus A proteion (SPA) labeled colloidal-gold is made into the colloidal-gold pad of the colloidal-gold antibody detection test strip. L7/L12 protein is a Brucella protein with high specificity and immunogenicity. L7/L12 gene is cloned from bovine Brucella genome and is connected to pET-28a to construct the prokaryotic expression recombinant plasmid, then the plasmid is transferred into the Escherichia coli to express L7/L12 protein, after the protein is purified, the purified protein is used as antigen to coat the nitrate cellulose film to be used as the check line (T line), and the check line and the colloidal-gold pad are assembled to the immune colloidal-gold detection teststrip of the invention. The test strip of the invention can be used for the detection of Brucella antibody in animal serum with strong specificity, high sensitivity, and good stability, and has considerable meaning and actual application value for the monitoring, diagnosis, purification, and control of the Brucella.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention discloses an animal brucella antibody colloidal gold test paper film detection kit, which bases on immunology basic principle of antigen and antibody specific combination, utilizes colloidal gold immunity chromatography technique and silver dye reinforcement technique, uses combined golden yellow staphylococcus A protein (SPA) as gold marking antibody, uses high purity brucella LPS as peridium antigen, and utilizes goat anti-bovine IgG as quality control line to prepare colloidal gold test paper tape. The invention can accurately differentiate species of animal brucella and can synchronously detect animal brucella disease of cattle, sheep and pig species with no crossing reaction and low false negative proportion, negative and positive coincidence ratio of the invention and conventional separation and culture is 100%, detection result can be obtained in 5 min, and the kit need no assistant complicated detection instrument.

The invention discloses a test paper card for testing a Brucella antibody through a competition method and belongs to the technical field of immunodetection. The test paper card is composed of an outer shell and a test paper strip in the outer shell. The outer shell is composed of an upper plate and a lower plate. The upper plate is provided with a testing window and a sample adding hole. The testing paper strip is composed of a base plate and a sample absorption pad, a colloidal gold signing pad and a testing reaction zone which are adhered to the base plate in sequence. The sample absorption pad is aligned to the sample adding hole and the testing reaction zone is aligned to the testing window. The invention further discloses a method for testing the Brucella antibody through the testing paper strip. The Brucella antibody in-site rapid testing paper card is high in sensitivity and repetitive rate, strong in specificity, good in stability, low in fake positive rate and fake negative rate, convenient to use, easy to observe and distinguish and suitable for clinical rapid diagnosis and epidemiological investigation large-scale application in a base level region, countryside and the like.

The invention discloses a brucella antibody gold-labeled fast detection kit, and belongs to the field of clinical medical detection. The kit consists of a card box and test strips, wherein the card box comprises a box cover (4) and a box bottom (7), and the detection test strips are arranged in the card box. A feeding hole (1) and a peep window (2) are formed in the front side of the card box, and a feeding hole marker (5), a result judgment interpretation (6) and an information record column (3) are printed on the front side of the box cover. The 'indirect method' detection kit is made by applying a colloidal gold immuno-chromatography technology, using an engineering expressed brucella BP26, OMP25 or OMP31 antigen as a detection antigen and using an efficient anti-human IgG monoclonal antibody as a capture antibody; the kit can be used for detecting antibodies of brucella in human serum, plasma or whole blood, and has the advantages that the detection method is simple, the result display is accurate and fast, and special instruments are not needed.

The invention discloses an application of lipopolysaccharide extracted from a brucella ovis vaccine strain M5 as a human brucellosis diagnosis antigen. According to the invention, the reactivity of anLPS antigen extracted from the brucella ovis vaccine strain M5 as a diagnosis antigen is obviously superior to that of an LPS antigen extracted from a brucella cattle strain S19 and a brucella pig strain S2 commonly used in a brucella antibody rapid detection kit on the market at present, therefore, the brucella ovis vaccine strain M5 can be used as a preferred source strain of a new brucella gold-labeled diagnosis LPS antigen, the extracted LPS has a good diagnosis value on human brucellosis, and a new way is provided for selection of a human brucellosis infection diagnosis antigen. The invention also finds that the antigenicity of the LPS antigen extracted from the brucella ovis vaccine strain M5 subjected to innocent treatment is not influenced.

The invention belongs to the technical field of biological detection, and discloses a brucella latex microsphere antibody detection card and a preparation method thereof. The brucella latex microsphere antibody detection card comprises a shell and a sample diluent used in cooperation with the shell, wherein a test strip is assembled in the shell and comprises a PVC bottom plate, a sample pad, a marking pad, a coating film and a water absorption pad are adhered to the PVC bottom plate, and the marking pad is a glass cellulose film and is coated with a coupling marker of recombinant streptococcus protein G and latex microspheres; and a coating membrane is a nitrocellulose membrane, and the coating membrane is coated with a detection line coated with brucella lipopolysaccharide and a quality control line coated with a mouse anti-His tag monoclonal antibody. The brucella latex microsphere antibody detection card is low in preparation cost, is convenient and rapid, has the advantages of no need of equipment and instruments and no need of professionals, can be simultaneously suitable for detecting brucella antibodies in serum or blood samples of cattle, sheep and pigs, and widens the application range.

The invention discloses a Brucella antibody gel detection kit. The kit comprises a kit body; a detection reagent card box with a built-in detection reagent card, a brucella antigen reagent bottle, a positive quality control reagent bottle, a negative quality control reagent bottle, a diluent reagent bottle and a multiple dilution plate are arranged in the kit body; and microsphere gel and an anti-human globulin antibody AHG are arranged in the detection reagent card. Compared with the prior art, the Brucella antibody gel detection kit has the following advantages: the anti-human globulin microsphere gel method in the brucella antibody gel reagent monitoring box enables the defect that incomplete antibodies cannot be detected by SAT and RB methods to be overcome; meanwhile, the cumbersome operation of washing and centrifuging for multiple times by a Coobs method is avoided; interpretation results are objective and standardized and are not affected by skills of operators, and the Brucella antibody gel reagent monitoring kit can detect whether a Brucella antibody exists in serum to be detected or not within one hour and further judge whether Brucella infection exists or not, which cannot be achieved by an immune capture agglutination method.

The invention discloses an ELISA kit for detecting a Brucella antibody. Engineering bacteria is recombined by Omp31 gene construction, expression Omp31 recombination protein is used as a coating antigen to fabricate protein coating plate, and an ELISA antibody detection kit is built. The kit has the advantages of good specificity, high sensitivity and low detection cost and is suitable for promotion. An important method is provided for aspects of antibody level detection of disease, epidemiological investigation, disease identification and effective prevention measurement taking. The kit mainly comprises an Omp31 protein coating plate, positive control serum, negative control serum, an enzyme-labeled rabbit-anti polyclonal antibody, a substrate liquid, a stop liquid, a diluent liquid, a washing liquid and a serum attenuation plate.

The invention relates to a diagnostic test strip for serological identification of Brucella antibody, which belongs to the field of immunology test. The invention comprises a locking shell and a teststrip. The locking shell comprises a locking cover and a locking seat which are fastened to each other. The test strip comprises a bottom plate, and an absorbent pad, a detection pad, a fluorescent microsphere pad 1, a fluorescent microsphere pad 2 and a sample pad which are successively lapped and adhered to the bottom plate. The invention is characterized in that: the test pad is a nitrocellulose membrane provided with a quality control line C, a detection line T1 and a detection line T2; the quality control line C is coated with anti-LPS polyclonal antibodies; the detection line T1 is coated with LPS antigens; the detection line T2 is coated with NH antigens; and the fluorescent microsphere pad 1 and the fluorescent microsphere pad 2 are NH antigens labeled with time-resolved fluorescent microspheres and glass cellulose membranes of LPS antigens respectively. The invention provides a new method for systematically, conveniently and standardizedly detecting the immune antibodies of the S2 vaccine and the infectious antibodies of the disease.

The invention provides a quantum dot immunochromatographic test strip for rapid detection of Brucella antibodies, which is prepared by pasting a sample pad, a binding pad, a chromatographic membrane, and a water-absorbing pad on a PVC base plate in sequence by 1-1.5mm overlapping each other , the chromatographic membrane is a solid-phase nitrocellulose membrane composed of a detection line and a quality control line, the detection line is coated with Brucella whole bacteria protein, and the quality control line is coated with a secondary antibody, The binding pad is coated with quantum dot-labeled Brucella whole bacterial protein, and the present invention also provides a method for preparing the test strip, which greatly simplifies the labeling steps and improves the labeling efficiency; the prepared test strip shortens the The detection time is long, and it also has the advantages of strong specificity, good stability, and less serum consumption. It can realize the detection of Brucella antibodies in human and animal serum, and is of great importance for the rapid on-site screening and diagnosis of Brucellosis. significance.

The invention discloses a Brucella antibody rapid quantitative detection card and detection method. The Brucella antibody rapid quantitative detection card comprises a PVC bottom plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad and a dilution pad which are installed in a shell. The nitrocellulose membrane is fixed to the PVC bottom plate, the left end of the nitrocellulose membrane is in lap joint with the water absorption pad, and the right end of the nitrocellulose membrane is in lap joint with the dilution pad; a marker pad is arranged at the right position of the middle part of the nitrocellulose membrane, a sample pad is arranged on the marker pad, and a quality control line and a detection line are arranged between the water absorption pad and the marker pad on the nitrocellulose membrane; the upper shell is provided with window holes corresponding to the detection line and the quality control line, one side of each window hole is provided with a quantitative identification line, a sample adding hole is formed corresponding to the sample pad, and a cleaning hole is formed corresponding to the dilution pad. According to the invention, the immune binding solution is accurately controlled by adding the sample into the sample adding hole, adding the diluent into the cleaning hole and arranging the quantitative marking line, so that not only can the detection sensitivity and precision be improved, but also the characteristics of rapidness, simplicity and convenience of the detection method are retained.

The invention belongs to the technical field of Brucella antibody detection, and particularly relates to a novel Brucella antibody detection method. According to the method, colloidal gold and latex microspheres are utilized to bridge a brucellosis antibody, the particle size is changed, the maximum absorption and scattering light wavelength change are detected through a spectrophotometer to identify the brucellosis infection condition. Colloidal gold and latex microspheres are respectively used for marking brucellosis outer membrane proteins, and form a 'cross-linked bridge' by capturing brucellosis antibodies, on the basis of an agglutination experiment, the agglutination principle of capturing brucellosis antibodies by adopting the colloidal gold and the latex microspheres is adopted, and a spectrophotometer method is combined for testing results, so that the detection sensitivity is greatly improved, and a result can be obtained within 3-5 minutes. The method has the advantages of high sensitivity, objective interpretation and simple operation, and is convenient for detection and use by basic level personnel.

The invention discloses an ELISA kit for detecting a Brucella antibody. An Omp31 gene is used for constructing recombinant engineering bacteria, an Omp31 expression recombinant protein is used as a coated antigen for preparing a protein coated plate, and the ELISA antibody detecting kit is established. The kit is good in specificity, high in sensibility, low in detection cost, and suitable for popularization. An important method is provided for a disease in aspects of antibody level detection, epidemiological investigation, similar disease identification and effective control measure adoptionand the like. The kit comprises the following main components: the Omp31 protein coated plate, positive control serum, negative control serum, an enzyme labeling rabbit anti-bovine polyclonal antibody, substrate liquid, a stop solution, a diluent, washing liquid, and a serologecal plate.