31 results about "Bovine Species" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

The present invention relates to a method of immune activation which is effective for eliciting a non-antigen-specific immune response in a member of the bovine species. The method is particularly effective for protecting a member of the bovine species from infectious disease and treating animals inflicted with infectious disease.

A method of indentifying brucella natural infection or immunifaction for livestock is provided. According to the method, Omp31 protein or recombinant Omp31 protein containing a His label is adopted as interspecific difference identification protein, and Bp26 protein or recombinant Bp26 protein containing a His label is adopted as positive control protein. For livestock positive to a brucella infection serum SAT, the Bp26 protein or the recombinant Bp26 protein containing the His label reacts with serums infected by different strains of brucella, while the Omp31 protein or the recombinant Omp31 protein containing the His label only reacts with serums infected by brucella except the bovine brucella, and therefore the livestock which is positive to the SAT is naturally infected or is subjected to immunifaction. Through Western Blot detection, the two kinds of protein can identify the bovine brucella immune serums and non-bovine brucella immune serums, and can identify natural infection and immunifaction of bovine. The Omp31 protein and the Bp26 protein can be used for indentifying nonspecific agglutination reactions of other microorganisms with a brucella SAT antigen.

The invention discloses a brucella molecular marker vaccine strain for bovine species and an application thereof. The brucella molecular marker vaccine strain for bovine species, which is provided by the invention, is obtained by replacing the coding gene of a protein bp26 of brucella BH1 with the coding gene of a BLS protein and the coding gene of a protein L7/L12 and inactivating the coding gene of a wboA protein of brucella. Experiments prove that the molecular marker vaccine strain BH1deltabp26deltawboA-BL has good immunoprotection effects on brucellosis, has lower toxicity and obviously improved safety, can be used for immunoprophylaxis of brucellosis of cattle and sheep, can distinguish wild strain infected animals from immune animals by adopting the immunological technique, has great significance in monitoring, diagnosing, purifying and controlling brucellosis and has extensive application value.

The invention relates to a cattle breeding method. According to the method, cattle breeds are selected, a mating time control point is managed, GnRH is injected, a calving time control point is managed, nutriments are supplied, and a weaning time control point is managed; breeding matching measures are taken between the mating time control point, the calving time control point and the weaning timecontrol point; healthy bulls are selected to be subjected to insemination mating with cows, wherein the mating time point is July to September in the current year; GnRH is injected to the cows 12-24hours before insemination mating; and during the pregnancy period, the cows are fed with fetus protection feed in which multivitamins, a polysaccharide-iron complex, choline chloride, folic acid, carrots and wheat are combined. The technology and method are comprehensive, the cattle breeding method is convenient to operate, the breeding potential of cows capable of breeding can be fully played, the number of the cattle breeds can be increased, and the breeding efficiency of the cattle breeds is effectively improved.

Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk. The invention also includes transgenic bovine species capable of producing recombinant polypeptides in transgenic milk as well as the milk from such transgenic bovine species and food formulations containing one or more recombinant polypeptide. Methods are also provided for producing transgenic non-human mammals having a desirable phenotype. The method comprises first methylating a transgene followed by introduction into fertilized oocytes. The oocytes are then cultured to form pre-implantation embryos. Thereafter, at least one cell is removed from each of the pre-implantation embryos and the DNA digested with a restriction endonuclease capable of cleaving the methylated transgene but incapable of cleaving the unmethylated form of the transgene. Those pre-implantation embryos which have integrated the transgene contain DNA which is resistant to cleavage by the restriction endonuclease in the region containing the transgene.

The invention provides an in vivo method whereby female offsprings can be produced in mammals, especially in bovine species said method comprising the step of administering a therapeutically effective amount of a material comprising acetic acid, or its pharmaceutically acceptable derivatives to female mammals just after or before insemination

The invention discloses a method for detecting bovine brucellosis by a competence ELISA (Enzyme-Linked Immunosorbent Assay) kit, belonging to the field of biotechnical detection. The method comprises the steps: coating bovine brucella A99 subjected to ultrasonic splitting by an ELISA enzyme-labeled plate; then adding an anti-brucella specific monoclonal antibody bru-5C10 and a diluted to-be-detected serum sample in plate holes of the ELISA coated plate; and simultaneously respectively adding the anti-brucella specific monoclonal antibody bru-5C10 and known negative and positive serum in the plate holes of the ELISA enzyme-labeled plate, washing with PBST (polybutylene terephthalate) after water bathing, respectively adding an anti-mouse IgG (Intravenous Gamma Globulin) enzyme-labeled antibody in each plate hole, performing a chromogenic reaction after incubation and washing, measuring an OD450 value of each plate hole, and calculating an inhibition rate of each plate. According to the method, yersinia enterocolitica O9 and escherichia coli O157 high-immunity positive serum are detected by using an established cELISA method, a PI (inhibition rate) value is lower than 30 percent, and the generation of a false positive result can be effectively eliminated.

The invention provides specific primers for detecting the mRNA expression level of bovine LPL (lipoprotein lipase) genes. The nucleotide sequence of the forward primer is 5'-CCGCAGACAGGATTACAG-3'; and the nucleotide sequence of the reverse primer is 5'-AGGAATGAGGTGGCAAGT-3'. The invention further provides a corresponding detecting kit. The kit fulfills the purpose of simply and quickly detecting the transcription level of the LPL genes, and can be used for monitoring the mRNA expression state of different bovine species (including dairy cow, cattle and yak), different tissues and different periods. The kit has the advantages of high sensitivity, good stability and low experimental cost on the aspect of detecting the mRNA expression level of the genes. The kit can be used for monitoring the mRNA expression level of the LPL genes in the fat deposition process of different bovine species, interpreting the fat deposition principle and identifying the correlation between the genes and the animal fat deposition and meat quality.

The invention belongs to the technical field of beef cattle fattening, particularly relates to a beef cattle fattening feeding method, and aims to solve the problems that beef cattle are very easy to get sick and the breeding benefit is not high due to the lack of the existing beef cattle feeding and management technology by farmers, the following scheme is provided: the method comprises the following steps: S1, selecting a proper site to build a cattle house; s2, selecting a proper cattle breed; s3, isolated feeding; s4, beef cattle fattening; and S5, breeding management: in the step S1, an environment with high terrain, good ventilation and good daylighting is selected to build a cowshed, a free site is built behind the cowshed, daylighting and cowshed drying are facilitated, and in the step S2, 3-6-month-old beef cattle which are uniform in structure, thick and strong in four limbs, long in body, wide in back and good in fur luster are selected. The beef cattle breeding method is convenient to operate, a good and comfortable environment can be provided for beef cattle, the sickness probability of the beef cattle can be reduced, the growth speed of the beef cattle can be increased, and breeding benefits can be improved.

An influenza C virus has been isolated from a bovine species. Influenza C virus polynucleotides and polypeptides have also been identified. Immunogenic compositions are also described, as well as diagnostic kits and methods of detection.

The invention discloses a bovine brucellosis polysaccharide conjugate vaccine and application thereof. An exogenous O-glycosylation system is expressed in O:9 serogroup yersinia enterocolitica, so that O-antigen polysaccharide of the O:9 serogroup yersinia enterocolitica is covalently linked to a substrate protein cholera toxin B subunit, and host bacteria are taken as glycosylation engineering bacteria to synthesize the polysaccharide conjugate vaccine. The polysaccharide structure of the glycoprotein is the same as that of bovine brucellosis, and the glycoprotein is expected to have a protective effect on bovine brucellosis in immune animals. Yersinia enterocolitica of the O:9 serogroup belongs to a secondary biosafety hazard organism and is easier to operate and culture than bovine brucellosis, so that the Yersinia enterocolitica is selected as a host bacterium to avoid large-scale culture of more dangerous bovine brucellosis, and the production process is safer and more efficient.