3 results about "Passive immunity" patented technology

The invention discloses a preparation method and application of a monoclonal antibody based on recombinant goose circovirus Cap protein, and belongs to the technical field of biology. The monoclonal antibody prepared based on the recombinant goose circovirus Cap protein has excellent specificity and affinity, has excellent reactivity with the goose circovirus, and also has high specificity and sensitivity when being applied to preparation of diagnostic reagents. Besides, the prepared monoclonal antibody is applied to non-therapeutic purpose neutralization virus activity and passive immune activity, and the monoclonal body can block the infection mechanism by combining virus key protein (Cap protein); the antibody targets the neutralizing epitope of the virus capsid protein and inhibits virus replication; instant immune protection can be provided by directly injecting the monoclonal antibody, interaction with virus protein can be achieved, neutralizing epitopes can be accurately positioned, vaccine design can be guided, the monoclonal antibody can be used for quantitative detection of the antibody level after vaccine immunization, and the monoclonal antibody has important significance in virus diagnosis and illness state evaluation.

This invention relates to the field of bioengineering, specifically to a method, apparatus, electronic device, and storage medium for screening key immune-related genes in calves. The screening method includes: acquiring transcriptome data from peripheral blood samples before and after colostrum in different calf samples; screening differentially expressed gene data based on the transcriptome data from the pre-colostrum and post-colostrum peripheral blood samples, and performing enrichment analysis to obtain gene sets related to specific biological processes, functions, or pathways; and then using weighted co-expression network (WCGNA) analysis to obtain key genes. This invention studies the gene expression regulation patterns and metabolite expression changes in calves before and after colostrum, providing a basis and theoretical foundation for further understanding the establishment and response mechanisms of the calf immune system, when the passive immune system terminates, and how the autoimmune system is regulated at the molecular level.

The present application relates to the field of biomedical technology, and particularly relates to a method for screening immune-related metabolites of a calf based on non-targeted metabolomics. The method comprises the following steps: S1. collecting peripheral blood of the calf at different time periods from birth to establishment of the immune system; S2. performing non-targeted metabolomics analysis on the peripheral blood samples at the different time periods to obtain differential metabolites at the different time periods; S3. annotating, clustering, performing enrichment analysis and performing overall change analysis of metabolic pathways on the differential metabolites at the different time periods screened out through a KEGG database to obtain key metabolites of the calf during establishment of the immune system and response mechanisms. The present application studies the expression change patterns of metabolic products of the calf before and after colostrum, understands the establishment process and response mechanisms of the immune system of the calf, and provides evidence and theoretical basis for when the passive immune system of the calf ends and how the immune system of the calf is regulated at the molecular level.