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Anti-adhesin based passive immunoprophlactic

a technology of anti-adhesin and immunoprophlactic, which is applied in the direction of antibacterial agents, drug compositions, antibody medical ingredients, etc., can solve the problem of not currently licensed effective vaccines against

Inactive Publication Date: 2006-11-30
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a method for preventing diarrhea caused by Escherichia coli (E. coli) by using a specific type of antibody found in cow's milk. This antibody can be administered as a food supplement or through a recombinant adhesin polypeptide given to cows. The technical effect of this invention is to provide a specific and effective way to protect against E. coli diarrhea until vaccines can be developed.

Problems solved by technology

However, there are no currently licensed effective vaccines against ETEC.

Method used

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Examples

Experimental program
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Effect test

example 1

Production of anti-CfaE Bovine Immunoglobulin

[0036] As mentioned above, the highly conserved nature of the amino-terminal β strand of CfaB and its homologs, together with other structure / function studies in type 1 fimbrial subunits, suggested this structure as a good candidate for the donor β strand that interlocks CFA / I subunits. In order to test this hypothesis with respect to the minoradhesive subunit, a plasmid was engineered to express a CfaE variant containing a C-terminal extension consisting of a flexible hairpin linker (DNKQ, SEQ ID No. 10) followed by an amino acid sequence of CfaB (FIG. 2). It was found that a CfaB donor strand length of at least 12 to as many as 19 amino acids was necessary to obtain a measurable recovery of CfaE. In studies using constructs containing a 12 to 19 amino acid donor strand, where mutations were introduced to break the β strand, it was demonstrated that the β strand is important to the observed stability achieved by the C-terminal amino aci...

example 2

Production of Anti-CfaD (CS17) Bovine Immunoglobulin

[0049] Use of other class 5 fimbrial adhesins are also contemplated as eliciting protective passive antibody production. As a further illustration, results of inhibition by antibody to CS17 (i.e., CsbD) is presented in FIG. 5. The antigen used to elicit antibody was a CsbD polypeptide (SEQ ID No. 22) expressing construct. The construct was engineered similar to that for CfaE, in Example 1, above but with a nucleotide sequence encoding CsbD (SEQ ID No. 19). The construct was designated dsc19CsbD[His]6. The donor strand consisted of 19 amino acids of CsbA (SEQ ID No. 7).

[0050] As can be seen in FIG. 5, like that for CfaE, antibody to CsbD was highly efficient at inhibiting MRHA. Also, like that observed for CfaE, anti-CsbD antibody also afforded cross-protection against CS4 and CS2.

[0051] The functional activity of BIgG to CS17 and CsbD was also evaluated, as in FIG. 4. These results are illustrated in FIG. 6. Like that observed f...

example 3

Specific Regions of ETEC Fimbrial Adhesin are Important for Immunoreactivity and Stability

[0052] Crystollgraphic analysis of the dscCfaE reveals that fimbrial adhesin is composed of two domains, an adhesin domain, formed by the amino-terminal segment of the adhesin molecule and a C-terminal pilin domain. The two domains are separated by a three amino acid linker. In an attempt to understand those regions of fimbrial adhesin, amino acid substitutions where made and the ensuing immunoreactivity analyzed. It was found that replacement of arginine 67 or arginine 181 with alanine, on CfaE abolishes the in vitro adherence phenotype of the molecule. These amino acids positions are located on exposed regions of the molecule with residue Arg 181 located on the distal portion of the amino-terminus of the domain. Therefore, this region of CfaE and the comparable region of the other fimbrial adhesins, is important for efficacious immune induction. Table 3 summarizes the positions in the eight ...

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Abstract

The invention relates to an immunogenic composition and method of the immunogenic composition for the production and administration of a passive immunoprophylactic against enterotoxigenic Escherichia coli. The immunoprophylactic is made collecting anti-adhesin in the colostrum or milk of vaccinated domesticated animals such as cows. The immunoprophylactic is administered either as a dietary supplement or in capsular or tablet form.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to U.S. Provisional Application No. 60 / 683,787 filed May 24, 2005.FIELD OF INVENTION [0002] This inventive subject matter relates to a pharmaceutical useful in conferring passive protection against diarrhea caused by enterotoxigenic Escherichia coli. SEQUENCE LISTING [0003] I hereby state that the information recorded in computer readable form is identical to the written sequence listing. BACKGROUND OF INVENTION [0004] Enterotoxigenic Escherichia coli (ETEC) are a principal cause of diarrhea in young children in resource-limited countries and also travelers to these areas (1, 2). ETEC produce disease by adherence to small intestinal epithelial cells and expression of a heat-labile (LT) and / or heat-stable (ST) enterotoxin (3). ETEC typically attach to host cells via filamentous bacterial surface structures known as colonization factors (CFs). More than 20 different CFs have been described, a minority of w...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/40A61K35/20
CPCA61K39/0258C07K2316/96C07K16/1232A61K2039/505C07K2317/76A61P1/12A61P31/04Y02A50/30
Inventor SAVARINO, STEPHEN
Owner THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
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