4 results about "Cold adapted" patented technology

This invention discloses a method for screening thermosensitive UDG rational mutation sites based on strain culture temperature. The method first divides strains into heat-tolerant and cold-adapted groups according to temperature thresholds based on the culture temperature of the strain preservation database, constructing corresponding UDG sequence sets and three-dimensional structure sets. Then, a first candidate mutation site set is generated through multiple sequence alignment and amino acid distribution difference analysis. A second candidate mutation site set is generated through rigid body alignment and functional protection rules under a unified coordinate system. Finally, multiple-source indicators such as structural dispersion, contact and salt bridge networks, and kinetic coupling are calculated for the second candidate mutation site set. Combined with effect-safety dual-dimensional scoring, spatial clustering, and cross-validation of the first candidate mutation site set, a preferred mutation site set is output. This invention achieves a systematic transformation from temperature ecotags to engineered sites, improving the reproducibility and interpretability of site screening without requiring a large-scale mutation library.

This invention discloses the application of the OsCAX1a gene in the breeding of cold-adapted rice varieties, belonging to the field of plant genetic engineering. The amino acid sequences of the two different spliceosome translation proteins encoded by the OsCAX1a gene are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively, and the CDS sequences are shown in SEQ ID NO.3 and SEQ ID NO.4, respectively. This invention reveals that OsCAX1a is a negative regulator of rice cold stress adaptation. Knocking out the OsCAX1a gene inhibits rice growth and development in the early stages, but as the heading stage lengthens and the plant enters the low-temperature growth period, the mutant exhibits significantly better cold adaptation, ultimately manifested in an increased number of tillers and increased grain yield per plant. By selecting varieties with low expression levels of this gene or by mutating the gene through gene editing, superior cold-adapted rice varieties tolerant to low-temperature stress can be cultivated, showing promising application prospects.

This invention discloses a cold-adapted xylose isomerase mutant, its preparation method, and its applications. Compared to the amino acid sequence of the wild-type xylose isomerase shown in SEQ ID NO: 1, the cold-adapted xylose isomerase mutant has a mutation at position 30, where alanine is replaced with glycine. This invention obtains a cold-adapted xylose isomerase mutant through rational design guided by ΔΔG on a heat-sourced xylose isomerase. This cold-adapted xylose isomerase mutant exhibits significantly enhanced activity at the fermentation temperature of *Saccharomyces cerevisiae*.

The present disclosure provides compositions and methods for manufacturing and obtaining a live attenuated Influenza vaccine (LAIV) composition that can be delivered intranasally to provide protection against influenza virus infection. Said LAIV strains are based on cold adapted, temperature sensitive and attenuated phenotypes of master donor viruses (MDVs) containing the surface glycoprotein genes of the wild type pandemic or seasonal influenza strains. Also, said LAIV strains are further adapted to grow in MDCK cells (Madin Darby canine kidney cells). The use of eggs is avoided in large scale vaccine manufacturing. The purification process is devoid of chromatography steps. The said LAIV composition includes one or more live attenuated influenza vaccine virus and is devoid of polymers and surfactants.