Extraction method of bovine tendon collagen

A technology of tendon collagen and collagen, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the defects of mechanical properties and biological stability of collagen materials, undisclosed process parameters, and unreachable Effectiveness and other issues, to achieve the effect of strong hydrophilicity, high extraction efficiency, and good product elasticity

Inactive Publication Date: 2016-04-27
FUJIAN HUAGENE BIOSCI CO LTD
View PDF9 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned method does not disclose specific process parameters, such as what kind of enzyme treatment and treatment time, and the existing preparation method has the following defects: trypsin, papain and pepsin are used in the enzymolysis process, and the reaction time is long. The yield is low, the purity is low, and the produced collagen material has defects in mechanical properties, biological stability, etc., which limit its practical application
In order to overcome the above shortcomings, the high voltage pulse method is also used to extract, but still can not achieve satisfactory results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method of bovine tendon collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] All operations in Example 1 were carried out in a sterile 10,000-level purification area, and the subpackaging and freeze-drying operations were all carried out in a local 100-level purification area, and the operations performed all met the operating requirements of the clean area.

[0035] (1) Take 2kg bovine tendon;

[0036] (2) Stir and wash the tendon with 2 times the volume of 0.3% (V / V) polysorbate 80 aqueous solution for 10 minutes, and rinse the tendon with water until the solution is clear;

[0037] (3) Stir and clean the tendon with 2 times the volume of 0.005M sodium hydroxide solution for 10 minutes, and rinse the tendon with water until the solution is neutral;

[0038] (4) Soak the tendon in water, and remove the adherent film on the tendon with curved scissors;

[0039] (5) Rinse with water until there is no debris;

[0040] (6) Arrange the cleaned tendons neatly, and freeze at -30°C for 30 minutes;

[0041] (7) Take out the tendon, cut it into 1-2mm ...

Embodiment 2

[0053] All operations in Example 2 were carried out in a sterile ten-thousand-grade purification area, wherein subpackaging and freeze-drying operations were all carried out in a local one-hundred-grade purification area, and the operations performed all met the operating requirements of the clean area.

[0054] (1) Take by weighing 5kg bovine tendon;

[0055] (2) Stir and wash the tendon with 2 times the volume of 0.3% (V / V) polysorbate 80 aqueous solution for 10 minutes, and rinse the tendon with water until the solution is clear;

[0056] (3) Stir and clean the tendon with 2 times the volume of 0.005M sodium hydroxide solution for 10 minutes, and rinse the tendon with water until the solution is neutral;

[0057] (4) Soak the tendon in water, and remove the adherent film on the tendon with curved scissors;

[0058] (5) Rinse with water until there is no debris;

[0059] (6) Arrange the cleaned tendons neatly, and freeze at -30°C for 30 minutes;

[0060] (7) Take out the ...

Embodiment 3

[0072] All operations in Example 3 were carried out in a ten thousand-class purification area, wherein subpackaging and freeze-drying operations were all carried out in a local one-hundred-class purification area, and the operations performed all met the operating requirements of the clean area.

[0073] (1) Take by weighing 10kg bovine tendon;

[0074] (2) Stir and wash the tendon with 2 times the volume of 0.3% (V / V) polysorbate 80 aqueous solution for 10 minutes, and rinse the tendon with water until the solution is clear;

[0075] (3) Stir and clean the tendon with 2 times the volume of 0.005M sodium hydroxide solution for 10 minutes, and rinse the tendon with water until the solution is neutral;

[0076] (4) Soak the tendon in water, and remove the adherent film on the tendon with curved scissors;

[0077] (5) Rinse with water until there is no debris;

[0078] (6) Arrange the cleaned tendons neatly, and freeze at -30°C for 30 minutes;

[0079] (7) Take out the tendon,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an extraction method of bovine tendon collagen. The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. The extraction method has the beneficial effects that the cold-adapted protease MCP-01 is used for hydrolyzing the bovine tendon in a buffer liquid system, so that collagen helix molecules of collagen micro fiber are exposed; collagen filaments expand and loose, so that the hydrolysis is fast and sufficient; and the yield and the product purity are further improved.

Description

technical field [0001] The invention relates to the field of medical bioengineering, in particular to a method for extracting bovine tendon collagen. Background technique [0002] Collagen is a biological polymer substance, a white, opaque, unbranched fibrous protein, mainly distributed in the connective tissue of mammals, and it is harmful to animal and human skin, blood vessels, bones, tendons, etc. Tendons, teeth, and cartilage are all important in the formation of these connective tissues. [0003] Most collagen is insoluble in water in its natural state and exists in the form of collagen fibrils or collagen fibers in the body. The basic structural unit of collagen fibrils is the procollagen molecule, and the peptide chains (subunits) are intertwined into a unique three-strand right-handed helical structure. Because the above structure is very stable, collagen is difficult to be biologically degraded, and only a few proteases can hydrolyze collagen fibers under specifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
CPCC12P21/06C07K14/78
Inventor 郑登忠黄发灿赵晶黄宏宝
Owner FUJIAN HUAGENE BIOSCI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap