Method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A manganese dioxide, biomolecular technology, applied in the fields of nano biomaterials and analytical chemistry, can solve the problems of volatile inactivation, poor stability of natural enzymes, etc.
Active Publication Date: 2016-11-09
XI AN JIAOTONG UNIV
View PDF8 Cites 34 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Therefore, the study of simulated enzymes can solve the problem...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0038] Based on the method of the present invention to detect reducing biomolecule-glutathione GSH
[0039] (1) Add 100μL of GSH solution of known concentration to 100μL of manganese dioxide nanosheet solution and 0.5mL of NaAc-HAc (0.2M) solution for 10 minutes at room temperature;
[0040] (2) Add the mixed solution in (1) to 200μL TMB solution (1.0mM) and react for 5min at room temperature;
[0041] (3) Detect the absorbance value of the solution in (2) at 650nm with a dual-beam UV-visible spectrophotometer, and mark it as A. Use ultrapure water instead of GSH solution as a blank experiment, and its absorbance value at 650nm is recorded as A 0 .
[0042] (4) With absorbance net signal △A=A 0 -A is used as a quantitative indicator to draw a GSH standard curve to obtain a linear equation (refer to figure 2 ) And detect the linear range (1μM-15μM) and detection limit (0.3μM) of GSH.
[0043] (5) Repeat the above steps (1), (2), (3) of the GSH solution to be tested to obtain the A valu...
Embodiment 2
[0046] Based on the method of the present invention to detect reducing biomolecules-ascorbic acid AA
[0047] (1) Add 100μL of AA solution of known concentration to 100μL of manganese dioxide nanosheet solution and 0.5mL of NaAc-HAc (0.2M) solution for 10 minutes at room temperature;
[0048] (2) Add the mixed solution in (1) to 200μL TMB solution (1.0mM) and react for 5min at room temperature;
[0049] (3) Detect the absorbance value of the solution in (2) at 650nm with a dual-beam UV-visible spectrophotometer, and mark it as A. Use ultrapure water instead of AA solution as a blank experiment, and its absorbance value at 650nm is recorded as A 0 .
[0050] (4) With absorbance net signal △A=A 0 -A is used as a quantitative indicator to draw the AA standard curve to obtain a linear equation (refer to image 3 ) And detection AA linear range (3-100μM) and detection limit (0.8μM).
[0051] (5) Repeat the above steps (1), (2), (3) of the AA solution to be tested to obtain the A value of A...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules. The peroxidase simulated by manganese dioxide nanosheet can perform catalytic oxidation on substrates of 3,3',5,5'-tetramethyl benzidine TMB, 2,2-azino-di(3-ethyl-benzothiazoles-6-sulfonic acid) diammonium salt ABTS and o-phenylenediamine OPD, and changes the color from colorless to blue, green and orange respectively, at the same time, the manganese dioxide nanosheet can sensitively and selectively perform an oxidation reduction reaction with reductive biological molecules such as glutathione and ascorbic acid, oxidation product concentration of the substrates such as TMB, ABTS and OPD is changed, and then, the reductive biological molecules such as glutathione and ascorbic acid are subjected to quantitative determination through a colorimetric analysis method. The method has the characteristics of simple operation, high sensitivity, good reappearance and high selectivity; a detection linear scope of glutathione is 1-15 [mu]M, the detection limit is 0.3 [mu]M; the detection linear scope of ascorbic acid is 3-100 [mu]M, the detection limit is 0.8 [mu]M; and the method can be used for detecting various phenolic compounds.
Description
Technical field [0001] The invention belongs to the technical field of nano-biological materials and analytical chemistry, and particularly relates to a method for detecting reducing biomolecules by manganese dioxide nano-sheet simulating oxidase. Background technique [0002] Enzymes are macromolecular substances with biocatalytic function, which have strong substrate specificity and high catalytic efficiency under mild conditions. However, the extraction and purification process of natural enzymes is time-consuming and complicated, high cost, and poor stability. At the same time, the catalytic activity of natural enzymes is easily denatured and inactivated by environmental influences, which severely restricts its further application. Mimic enzymes are substances with similar functions to natural enzymes, and can still maintain high stability under harsher conditions. Therefore, the research on mimic enzymes can solve the problems of poor stability and volatile inactivation of ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more