191 results about "Colorimetric analysis" patented technology

A cross-platform web-based system for water testing capable of measuring, analyzing and maintaining the quality of water using a plurality of different test strip readers each configured to obtain a digital image of a reagent test strip, normalize and analyze color information in the digital image by colorimetric analysis, and to transmit a set of colorimetric values corresponding to said digital image to a cross-platform cloud-based system for analysis. The cloud-based server(s) receive the colorimetric values, calculate analyte parts-per-million (ppm), and return the calculated analyte ppm with consistant results no matter which reader is used.

The invention relates to a method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules. The peroxidase simulated by manganese dioxide nanosheet can perform catalytic oxidation on substrates of 3,3',5,5'-tetramethyl benzidine TMB, 2,2-azino-di(3-ethyl-benzothiazoles-6-sulfonic acid) diammonium salt ABTS and o-phenylenediamine OPD, and changes the color from colorless to blue, green and orange respectively, at the same time, the manganese dioxide nanosheet can sensitively and selectively perform an oxidation reduction reaction with reductive biological molecules such as glutathione and ascorbic acid, oxidation product concentration of the substrates such as TMB, ABTS and OPD is changed, and then, the reductive biological molecules such as glutathione and ascorbic acid are subjected to quantitative determination through a colorimetric analysis method. The method has the characteristics of simple operation, high sensitivity, good reappearance and high selectivity; a detection linear scope of glutathione is 1-15 [mu]M, the detection limit is 0.3 [mu]M; the detection linear scope of ascorbic acid is 3-100 [mu]M, the detection limit is 0.8 [mu]M; and the method can be used for detecting various phenolic compounds.

The invention relates to fluorescent colorimetric analysis and discloses a colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment. The colorimetric analysis method comprises the following steps: reacting a copper ion solution and a Rhodamine fluorescent indicator synthesized in a lab to enhance fluorescence, and exciting the indicator through light with a particular wavelength of 365nm by adopting home-made fluorescent colorimetric equipment; catching fluorescence color change of the indicator before and after the copper ions are added by using electronic imaging equipment such as a scanner and a digital camera; extracting color change values of red, green and blue (RGB) channels in the image before and after color change, and establishing a standard curve between the copper ions and a concentration gradient which corresponds to the copper ions; and comparing the obtained color change values of the sample with values in the curve in a sample test process, thus quantitatively and selectively analyzing the copper ions in an unknown sample. According to the method, copper ion concentration of 10<-5>M can be detected, co-existing interference of nine ions can be effectively eliminated, and the method has high selectivity.

The invention relates to preparation and application of a paper-based microfluidic chip for colorimetric analysis; the preparation comprises the step of enabling a hydrophilic channel to be jointed on a hydrophobic substrate, wherein the hydrophilic channel is obtained by printing the pattern of the channel on a piece of filter paper and then shearing the pattern, and the hydrophobic substrate is obtained by treating the filter paper with a normal hexane solution containing methyl trichlorosilane and octadecyl trichlorosilane; the paper-based microfluidic chip is combined with a colorimetric analysis method, so that rapid quantitative detection can be realized. The preparation method is simple and fast, short in production cycle, low in cost, low in energy consumption and free from pollution. The preparation and the application of the paper-based microfluidic chip for colorimetric analysis are suitable for the fields such as clinical diagnosis, food hygiene, environmental monitoring and biochemistry, thus having very wide application scope.

The present invention provides an automatic analyzer making it possible to reduce the cost for serum information measurement and prevent a decrease in processing capacity. An automatic analyzer comprises a colorimetric analysis section provided with a reaction vessel to mix a sample with a reagent and a measurement section to measure the color change of the mixture in the reaction vessel; and an electrolyte measurement section to measure the concentrations of electrolytes in a sample, wherein the sample included in the reaction vessel is diluted to be measured in the measurement section of the colorimetric analysis section, and at least one of the degrees of hemolysis, lipemia and icterus in a sample is measured in the electrolyte measurement section.

The invention relates to a colorimetric analysis method for measuring the content of glucose and the activity of glucose oxidase, which is an efficient, quick and sensitive colorimetric method for measuring the content of the glucose or analyzing the activity of the glucose oxidase in a sample. The evaluation system has the characteristics of simple and practical method, mild reaction system, friendly environment, less sample consumption and good analytical repeatability; and in addition, the condition required for analysis is not high, and accurate analysis can be realized only by utilizing the ordinary ultraviolet-visible spectrophotometer in a general laboratory. Compared with the conventional o-diaminobenzene method, the Fitton method and the electrochemical method, the colorimetric analysis method can avoid trivial operation steps effectively, reduce the consumption of time and manual labor, and reduce the influence of environmental factors to the sensitivity of detecting enzymatic activity effectively. Therefore, the novel glucose oxidase analysis method can be widely applied in the fields of food production, brewing, beverages, medical detection and eassay, biochemical research and the like.

The invention discloses a colorimetric analysis method for detecting kanamycin based on aptamer-modified magnetic beads and gold nanoparticles to simulate enzyme activity, and belongs to the technical field of analytical chemistry. In the present invention, the gold nanoparticles synthesized by tyrosine reduction of chloroauric acid simulate the peroxidase-like activity inherent in the enzyme AuNPs, and the gold nanoparticles modified by kanamycin-specific aptamers modify their complementary single-stranded cDNA The capture of nanoparticles, and the displacement of gold nanoparticles by the combination of kanamycin and aptamer, the peroxidation-like peroxidation of gold nanoparticles AuNPs contained in the supernatant after magnetic separation is related to the concentration of kanamycin The enzyme activity catalyzes the color reaction between the substrate tetramethylbenzidine (TMB) and H2O2, realizes the visual detection of kanamycin, and uses the linear relationship between the absorption value at 450 nm and the concentration of kanamycin to realize the detection of kanamycin. Colorimetric quantitative analysis of kanamycin. This method has the advantages of high sensitivity and good specificity, and is suitable for the quantitative analysis of kanamycin residues in food samples such as honey.

The invention relates to a method for detecting 1,5-dehydration glucitol in blood and a kit, and provides a new method for detecting the content of the 1,5-dehydration glucitol in the blood, which comprises the following steps of: catalyzing the 1,5-dehydration glucitol by using pyranose oxidase to generate 1,5-dehydration fructose and H2O2; generating quinine compounds by using 4-aminoantipyrine(4-AAP), 3-hydroxy-2,4,6-trihydroxybenzoic acid (HTIB) and H2O2 under the catalytic action of horse radish peroxidase; and determining the level of the 1,5-dehydration glucitol in the blood by colorimetric analysis. The invention also provides the kit for the method. The method and the kit are easy and convenient to operate safely, long in stable time and high in interference resistance, specificity and sensitivity, the interference of multiple glucoses in blood samples can be eliminated quickly, and the reliable foundation can be provided for the diagnosis and treatment of diabetes.

A method of detecting the amount of sulfur compounds in fuels such as ultra low sulfur diesel (ULSD) fuels is provided in which a fuel sample is reacted with a solvent and an oxidizing agent to produce a reaction product which may be analyzed by visual observation and / or in combination with spectrophotometric or colorimetric analysis. The oxidizing agent may be selected from potassium permanganate, sodium dichromate, nitric acid, hydrogen peroxide, cumene hydroperoxide, and sodium hypochlorite, and may be used in combination with an acid.

The invention discloses a method for measuring alkaline phosphatase activity. According to the method, L-ascorbic acid-2-phosphate is taken as a catalytic substrate of alkaline phosphatase, the substrate is hydrolyzed to form L-ascorbic acid under the catalysis of alkaline phosphatase, L-ascorbic acid can reduce bivalent copper into monovalent copper, a bright yellow bivalent copper-bathophenanthroline compound is converted into deep green monovalent copper-bathophenanthroline compound, the higher the alkaline phosphatase activity is, the more obviously the color of a detection liquid changes, and accordingly, semiquantitative colorimetric detection of the alkaline phosphatase activity is realized or quantitative detection is realized through an ultraviolet-visible spectrophotometer. By means of the method, alkaline phosphatase with limit of detection being 3.05 mU*mL<-1> can be detected. Compared with conventional methods, the method has high sensitivity and anti-interference capability and good repeatability, and is simple to operate, short in detection time and low in cost, and a simple, convenient, low-cost, high-resolution and sensitive colorimetric analysis method with stable performance is provided for detection of the alkaline phosphatase activity in a clinic sample.

Disclosed are a water quality analyzer and a method for analyzing water quality. The water quality analyzer includes a first disc system, a second disc system, a colorimetric system, a cleaning system, a mechanical sampling system, an analysis system and a central control display. The first disc system and the second disc system are axially rotatable. A plurality of sample locating positions and a chemical locating positions are provided on the first disc system along a circumference of the first disc system. A plurality of colorimetric cuvette locating positions are provided on the second disc system, and the colorimetric system is arranged at a circumference edge of the second disc system. The cleaning system and the mechanical sampling system are provided between the first disc system and the second disc system. The method includes water sampling, water sample injection, cleaning, reagent extraction, reagent injection, cleaning and colorimetric analysis.

The invention discloses a colorimetric analysis method for simply and rapidly detecting mercury ions in an aqueous solution. According to the method, the characteristic of high light absorption coefficient of nanogold is utilized, the nanogold is used as a chromogenic signal component, and simultaneously mercaptoethylamine is used as a specificity identification component. The colorimetric analysis method has the advantages of being low in cost, simple in operation, convenient and rapid. The modified nanogold is stable, high in resistance to high concentration salt ions, slightly interfered by external factors and suitable for being popularized and applied.

The invention provides an automatic sample feed system and a control method thereof. The automatic sample feed system comprise an automatic sample feed device and a control circuit thereof, wherein the automatic sample feed device comprises a sample pool, an agent pool, a three-way connector, a three-way electromagnetic valve, a sample mixing pool device and relative driving and controlling devices. According to a preset control program of the control circuit, the water sample of the sample pool and the agent of the agent pool are fed by peristaltic pumps to pass the three-way connector and the three-way electromagnetic valve to be filled into a sample mixing pool of the sample mixing pool device, and the injection pump head of the sample mixing pool device feeds the reacted mixture sample of the mixing sample pool via the three-way electromagnetic valve to enter into a detection device to process trace element and nutrient colorimetric analysis tests. The invention relates to the technical problems of the prior art such as automatic accurate proportioning of the trace elements and the nutrients of water in on-site online automatic colorimetric detection, sample feed and mixing reaction, to realize online detection on the trace elements and the nutrients of water.

The invention discloses a water quality analyzer. The water quality analyzer comprises a first disc system, a second disc system, a colorimetry system, a cleaning system, a mechanical sampling system, an analysis system and a center control display screen. The first disc system and the second disc system can rotate axially, several sample installation sites and agent installation sites are arranged on the circumference of the first disc system, several colorimetry ware installation sites are arranged on the second disc system, the colorimetry system is arranged at the circumference edge of the second disc system, the cleaning system and the mechanical sampling system are arranged between the first disc system and the second disc system, and the analysis system is connected with the colorimetry system and used for transmitting water quality parameters onto the center control display screen. The invention further discloses a water quality analysis method. The water quality analysis method includes the steps of water sample drawing, water sample injection, cleaning, agent taking, agent injection, cleaning, colorimetric analysis and the like. The water quality analyzer and the water quality analysis method have the advantages of high water quality analysis speed and large analysis parameter quantity and are suitable for application and popularization.

The invention relates to measurement of peroxide values, and in particular relates to an edible oil peroxide value measurement combined reagent and a detection method. The edible oil peroxide value measurement combined reagent comprises a polarity organic solvent, a reducing agent, a color development reagent and a color development stabilizing agent, wherein the polarity organic solvent comprises ethanol with the mass concentration of 237-710 g / L in percentage by mass; the reducing agent comprises ferrous sulfate with the mass concentration of 1-20 g / L in percentage by weight; the color development reagent is a sulfosalicylic acid solution with the mass concentration of 300-1,000 g / L; and the color development stabilizing agent is a pyridoxamine dihydrochloride solution with the mass concentration of 20-800 g / L. By a colorimetric analysis method, the operation is quick and simple, and interference caused by heating on a detection result is reduced; the types of the needed detection reagents is few, and the reagents are convenient to combine and carry; and the edible oil peroxide value measurement combined reagent can develop and detect a plurality of oil samples at the same time, is uniform in developing, is low in toxicity and even non-toxic, and is safe and environmentally-friendly.

Semi-quantitative colorimetric analysis of a fibrous matrix disposed on a support, and quantitative analysis of liquid color, are described. Cyanide and a halogenating reagent are reacted to produce a cyanogen halide, and Konig reagents are reacted with the cyanogen halide to yield a colorimetrically analyzable complex. A beneficial photometric method is also described. In addition, a reagent delivery device that includes a beneficial fibrous matrix for rapid delivery of reagents and rapid liquid color analysis, is described.

The invention provides substituted tricarbonal chloride [2-(2-pyridyl) benzimidazole] co-rhenium complexes or substituted tricarbonal chloride (2,2'-dipyridyl amine) co-rhenium, which are tricarbonal chloride [1-(4-5'-phenyl-1,3,4-oxadiazole phenyl)-2-(2-pyridyl) benzimidazole] co-rhenium (I), tricarbonal chloride [1-(4-N-carbazolyl phenyl)-2-(2-pyridyl) benzimidazole] co-rhenium (I), tricarbonal chloride [N-(4-5'-phenyl-1,3,4-oxadiazole phenyl)-2,2'-dipyridyl amine] rhenium co (I) and tricarbonal chloride [N-(4-N-carbazolyl phenyl)-2,2'-dipyridyl amine] co-rhenium (I). The complexes have a structural formula as follows. The tricarbonal rhenium (I) complexes having the advantages of simple synthesis, help to purification, high yield and stable properties in air can be applied to molecular catalysis, solar energy conversion, colorimetric analysis, molecular recognition, supramolecular assembly, optical information storage, photoluminescence probes in organisms, and other fields. The invention discloses a preparation method for the complexes.

The invention discloses a multi-cuvette automatic analysis system and method and application of the system and the method. The multi-cuvette automatic analysis system comprises a power device, a colorimetric device, a sample adding device, a cleaning device and a control device, wherein the power device is used for providing power for the multi-cuvette automatic analysis system; the colorimetric device is used for performing colorimetric analysis on samples; the sample adding device is used for adding the samples into cuvettes; the cleaning device is used for cleaning the cuvettes; the control device is used for regulating the parameters of each device; and the colorimetric device comprises the cuvettes of which the light paths are 1cm. The light paths of the adopted cuvettes are 1cm, the light paths are lengthened, and the colorimetric analysis of the low-concentration samples is realized.

The invention belongs to the technical field of mercury ion detection, and in particular, relates to a paper chip colorimetric analysis system for mercury ion detection and a construction method and an application thereof. The construction of the paper chip colorimetric analysis system includes the steps of preparation of a paper chip plastic bearing substrate, and design and preparation of the paper chip; the paper chip is similar to a micro reaction vessel and is used for platinum nanoparticle catalysis of a TMB / H2O2 developing reaction; the construction of a simple optical fiber analysis device is used for representation of the intensity of a color in the paper chip in a numerical value form. A principle comprises that platinum nanoparticles have excellent activity similar to catalase and can rapidly catalyze a TMB / H2O2 substrate to generate a blue precipitate, while when mercury ions exist, PtNPs can quickly undergo a reaction with the mercury ions to inhibit self enzyme activity, and finally, the recognition and quantitation of the mercury ions in the solution are achieved through changes of the color after the reaction. The system has the advantages of excellent specificity and selectivity, and has wide application prospect.

The invention relates to colorimetric analysis and provides a filtration colorimetric analysis method used for highly sensitive and selective detection of trace nickel ions in a solution. According to the invention, a nickel ion indicator reacts with a solution containing trace nickel ions so as to produce precipitate suspendingly dispersing in the solution, the solution containing the precipitate is pumped into a flow cell by a peristaltic pump, and the solution is filtrated after passing through a microporous membrane stuck in the middle of the flow cell; an electronic imaging device is used to capture colors of the membrane before and after the precipitate is filtrated; change values of a green channel in three channels, i.e., a red channel, the green channel and a blue channel, of images obtained before and after the color of the membrane is changed are extracted, and a concentration gradient standard curve between nickel ions and the change values of the green channel is constructed; in the process of testing of a sample, obtained change values of a green channel of the sample are compared to values in the curve, thereby realizing quantitative selective analysis of nickel ions in the unknown sample. The method provided in the invention can detect nickel ions with a concentration as low as nM-grade, can effectively suppress coexistence-interference of as many as 18 kinds of ions and has high selectivity.

The invention discloses a method for carrying out illegal cooking oil detection by using an ultraviolet spectroscopic analysis method. The method comprises the steps that: (1) an oil sample to be detected is subjected to pretreatment; (2) a standard curve is drawn by using a substrate standard addition method; a mixed oil product and a standard normal oil product are respectively pretreated by using the method provided by the step (1), and are scanned by using an ultraviolet spectrum colorimetric analysis method at wavelengths of 200-320nm; aqueous solution spectral absorption diagrams of the two samples are drawn and are adopted as standard curves of the two which are detection limits of illegal cooking oil detection; (3) a micro-emulsion liquid of oil in water molecules (W / O) is scanned by using an ultraviolet spectrum colorimetric analysis method at wavelengths of 200-320nm; an aqueous solution spectral absorption diagram of an oil sample to be detected is drawn; and whether the detected oil sample is illegal cooking oil is determined according to absorption value volume, spectral characteristics curve, and curve graphic similarity of the aqueous solution spectral absorption diagrams. Through many tests and validations, the success rate of the method can substantially reach 70-80%.

The invention relates to preparation and application of an aptamer colorimetric sensor for polychlorinated biphenyl 77 detection. An aptamer with a certain base sequence and specific PCB (polychlorinated biphenyl) 77 recognition capability and the colorimetric analysis technology are combined for constructing the PCB 77 aptamer colorimetric sensor. Due to adoption of the specific PCB 77 recognition aptamer as a recognition element, sensitivity and selectivity of the colorimetric sensor in PCB 77 detection are greatly improved. A PCB 77 detection linear range is 5*10<-10>-9*10<-7>mol / L, and a detection limit reaches 5*10<-11>mol / L. Compared with the prior art, the method has advantages of simplicity and convenience in operation, quickness in response, easiness in online detection and the like.

The invention relates to a color detection device and method of a portable paper-base micro fluidic chip; the paper-base chip is placed in a chip card slot, the chip card slot is arranged in the device; a LED light source emitted by a light emitter irradiates a reaction region of the paper-base chip to reflect out reflected light; a color detection sensor is connected with a signal amplifying unit, is used for receiving the reflected light, and converts a light signal into an electrical signal to sent into the digital amplifier unit for signal amplification processing; a digital-to-analog conversion unit is connected with the signal amplifying unit and a programmable controller, is used for receiving an amplified digital electrical signal, and converts the digital electrical signal into an analog electrical signal to sent into the programmable controller for data processing. The color detection device is a new microfluidic device designed on the basis of the principle of light reflection on paper, is used for colorimetric analysis of biochemical reaction related color to provide quantitative analysis data, and can be widely used in the fields of family disease rapid diagnosis, on-site instant detection and the like.

The invention relates to a method for rapidly detecting the total concentration of chromium in water. The method comprises the following steps that water to be detected is sampled, and the pH value of the water sample to be detected is detected through a pH test strip and is adjusted; 5 ml of the water sample to be detected is placed in a prefabricated tube containing 2 ml of potassium persulfate solution in advance, and 0.2 ml of copper sulfate solution is added to the water sample to be detected; a heating digester is switched on, the temperature is set to be 165 DEG C, after the temperature reaches 165 DEG C, the prefabricated tube containing the mixed water sample is inserted into a heating digestion groove, the prefabricated tube is sealed, and then digestion lasts for 10 minutes; after digestion, the prefabricated tube is taken out and cooled to the indoor temperature, 0.05 ml of phosphoric acid solution is added, and 0.2 ml of diphenylcarbazide color-developing solution is added after even mixing; after the mixed solution stands for 10 minutes, the prefabricated tube containing the mixed solution is inserted into a colorimetric groove of a photometer, a blank is used as a contrast, and colorimetric analysis is conducted at the position with the distance of 540 nm; the absorbance of the water sample to be detected is substituted into a total chromium concentration standard curve and is multiplied by dilution ratio, and then the total concentration of chromium is obtained. According to the method for rapidly detecting the total concentration of chromium in water, the detection speed is high, and the application quantity of a poisonous agent is small. The invention further provides a detection kit which is convenient to carry and use and is used for the method for rapidly detecting the total concentration of chromium in water.

The invention discloses a nanometer gold colorimetry method for rapidly detecting lead ions. According to the present invention, a boron cluster compound is used as a reducing agent and a stabilizer,a AuNPs-B12H12<2-> solution is prepared by using a chemical reduction method, nanometer gold is used as a coloring signal element due to the high absorption coefficient of the nanometer gold, the pH value of the AuNPs-B12H12<2-> solution is adjusted to 10-12, an electrolyte is added to make the AuNPs-B12H12<2-> solution in a metastable state, and then a solution to be detected is added, such thatthe Pb<2+> in the solution can be quickly, efficiently and specifically detected; and the colorimetric analysis method has advantages of simple preparation, no purification treatment, no requirement of further modification, long-term storage at the room temperature, low hetero-ion-induced interference, high Pb<2+> detection sensitivity and the like.

Methods for determining the concentration of nitrates in several types of water samples by photochemical reduction without the use of toxic materials such as cadmium or hydrazine, and having an approximately 100% reduction efficiency are described. A water sample mixed with a buffered aqueous solution including ammonium salts and EDTA is irradiated using ultraviolet light having wavelengths effective for photochemical conversion of nitrate and nitrite ions (NOx) to detectable species. The resulting species may be quantitatively determined by diazotization using sulfanilamide followed by coupling with N-(1-napthyl)ethylenediamine dihydrochloride which produces a water-soluble azo dye having a magenta color which may be colorimetrically measured at 540 nm from which the nitrate and nitrite concentration in the sample is determined. Nitrite present in the original sample may be colorimetrically analyzed using an unphotolyzed sample and the reaction with sulfanilamide followed by coupling with N-(1-napthyl)ethylenediamine dihydrochloride. When the nitrite concentration is subtracted from the total of the nitrate and nitrite concentration, the sample nitrate may be determined.

The invention provides a rail-type full-automatic biochemical analyzer with a plurality of reagent needles, which comprises a colorimetric cup, a movable platform for bearing the colorimetric cup, a sample cup rail for placing a sample cup, a sample adding device arranged above the movable platform, a reagent adding device and a colorimetric analysis device. The rail-type full-automatic biochemical analyzer with a plurality of reagent needles is characterized in that the rectangular movable platform is arranged on the rail, the motion direction of the sample cup rail is parallel to that of the movable platform, the reagent adding device comprises more than two reagent bottles, more than two precise reagent pumps and reagent needles, and the reagent needles comprise more than two reagent needle units. A circumferential motion mode is changed into a linear motion mode so that reagent addition, sample addition and colorimetric analysis are more convenient to control and the control precision is more convenient to keep. The working efficiency of the analyzer is improved by arranging more than two reagent needle units on the reagent needles.

The invention relates to a method for rapidly detecting organic phosphorus pesticide residues through a microchannel based on scanning and colorimetric analysis. The method comprises the following steps: preparing the organic phosphorus pesticide residue microchannel; adding a pesticide-residue rapidly-detecting reagent into the organic phosphorus pesticide residue microchannel; scanning to obtain the digital pictures for detecting the organic phosphorus pesticide residue microchannel; conducting colorimetrical analysis on the digital pictures of the microchannel for detecting organic phosphorus pesticide residues. Based on scanning and colorimetric analysis, the microchannel is used for rapidly detecting organic phosphorus pesticide residues. The microchannel has the advantages of multiple-flux detection, reusability and low cost, has high transparency, and greatly increases the precision of detection on organic phosphorus.

The invention discloses a clinical chemistry integrated and combined multi-reagent-strip detection device and method. The detection device comprises reagent strips, sampling needles, an optical detection system and a displacement device, the sampling needles are installed on the displacement device, each reagent strip is provided with multiple reagent cavities which are connected in parallel into a whole body, and the reagent cavities are located on the movement track of the displacement device. The method comprises the steps of sample suction, R1 reaction, R2 adding, colorimetric analysis, needle cleaning and the like. According to the clinical chemistry integrated and combined multi-reagent-strip detection device and method, the reagent strip structure that the multiple reagent cavities are connected in parallel, the detection procedure can be simplified, an automatic analyser can be compact in structure and high in reliability, and the detection for a small batch of samples is accelerated.