72 results about "Colorimetric analysis" patented technology

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention provides a peritoneal dialysis solution detection data intelligent analysis method based on visual identification, and the method comprises the steps: obtaining a to-be-identified test paper image, carrying out the test paper region segmentation and color correction of the test paper image, and carrying out the brightness equalization processing of a test paper detection region, and obtaining a processed test paper detection region; obtaining a standardized color feature vector of a test paper color development area from the test paper detection area; carrying out mapping matching on the standardized color feature vector and a standard colorimetric database to obtain a colorimetric matching value, carrying out color abnormality identification according to the colorimetric matching value, comparing the color abnormality with a preset abnormal value to obtain a test paper colorimetric analysis result, and carrying out self-adaptive correction on the test paper colorimetric analysis result with abnormality; and constructing a personalized colorimetric model according to the colorimetric matching value in combination with a historical colorimetric matching value, and performing health trend analysis and early warning.

A blood testing device for detecting hemolysis in a blood sample is described. The blood testing device comprises an housing for containing the blood sample. The housing has a treatment window and an optical zone formed therein. The blood testing device further includes an acoustic transducer positioned to selectively generate acoustic forces directed into the treatment window of the housing and a control unit for selectively actuating and deactuating the acoustic transducer to permit colorimetric analysis of plasma within the blood sample.

This invention relates to biodetection technology, disclosing a peptide-modified gold nanocluster, its preparation method, and its application in the detection of microplastics. The peptide-modified gold nanocluster comprises a gold nanocluster and a peptide LCI modified on the surface of the gold nanocluster, wherein the peptide LCI comprises an amino acid sequence as shown in SEQ ID NO: 1. The preparation method includes the following steps: providing functionalized modified gold nanoclusters; reacting the functionalized gold nanoclusters with the peptide LCI to attach the peptide LCI to the surface of the gold nanoclusters, forming the peptide-modified gold nanocluster. The microplastic detection method includes mixing the peptide-modified gold nanocluster with a test sample that may contain microplastics to obtain a detection solution, and performing colorimetric analysis or absorbance analysis on the detection solution. This gold nanocluster can specifically recognize polypropylene, exhibiting good specificity, low cost, and rapid detection of polypropylene microplastics.

The utility model belongs to the agricultural product pesticide residue detection technical field discloses a device for detecting agricultural product pesticide residue, including base frame, the base of base frame is opened and has colorimetric card placing groove and optical magnifying glass placing groove, broken jar, broken jar includes broken jar body and the jar cover of sleeve joint installation in the top of broken jar body, the broken structure is integrated on the jar cover, conveying chamber, conveying chamber is installed in the bottom of broken jar body, and the bottom of conveying chamber is threadedly matched and is connected with filter cover, filtrate container, filtrate container can be detachably installed on the base of base frame, and filtrate container is located the just below of conveying chamber, and the base of base frame is opened and has the clamping groove corresponding with filtrate container, and the clamping groove is adapted to filtrate container. The utility model breaks through the space limit of traditional laboratory equipment by integrating the compact base frame of broken, filter, colorimetric analysis function, supports the field fast screening of field, exempts power dependence simultaneously manual operation design, reduces use cost significantly.

The invention discloses an Hg < 2 + > detection method for enhancing Hg < 2 + > intrinsic laccase-like activity based on ZIF-8-Au. The invention finds that the mercury ions have laccase-like activity, the enzyme activity of the mercury ions is obviously enhanced under the action of ZIF-8-Au, and the color of the reaction catalyzed by the mercury ions is obviously changed along with the increase of the concentration of Hg < 2 + >, so that a colorimetric analysis method for detecting the mercury ions is developed on the basis, the Turn-On type visual analysis is realized, and the application prospect is wide. The method realizes simple, rapid, sensitive and high-selectivity detection of Hg < 2 + > residues in water and food. The invention provides a new method and principle for the fields of Hg < 2 + > environment monitoring and food safety, and provides a new direction for the development of a heavy metal ion detection technology.

The invention discloses fresh pesticide residue detection equipment and a use method thereof, and belongs to the technical field of food safety detection. The equipment comprises a rack structure, a charging barrel structure I, a charging barrel structure II and a detection box body, and integrates the functions of cleaning, crushing, extracting, filtering, reacting, colorimetric analysis and the like. A sample is subjected to rotary spraying type cleaning through the cleaning structure, is crushed through the crushing structure, is mixed with an extraction liquid, is subjected to negative pressure suction filtration, and finally enters the detection module to complete enzyme inhibition reaction and colorimetric detection. A detection result is output in real time through a built-in display screen, an alarm is automatically given when the detection result exceeds the standard, and data are supported to be uploaded to a cloud platform. The equipment is compact in structure, high in function integration degree, simple and convenient to operate and suitable for rapidly detecting various pesticide residues in agricultural products such as vegetables and fruits on site, the detection efficiency and precision are improved, and the rapid detection requirements of basic layers and circulation links are met.

The application discloses a kind of ratio fluorescent sensor based on smart phone and its preparation method and application, the preparation method includes: after mixing chloroauric acid, bovine serum albumin, sodium hydroxide, gold cluster reaction liquid is obtained;Sodium citrate, 3-aminopropyl triethoxysilane, glycerol are mixed to obtain silicon quantum dot solution;Silicon quantum dot solution, lipopolysaccharide aptamer, EDC, NHS are reacted in ultrapure water to obtain aptamer modified silicon quantum dots;Lipopolysaccharide aptamer modified silicon quantum dots and gold cluster are mixed to obtain ratio fluorescent sensor.The ratio fluorescent sensor based on smart phone prepared by the application has good stability, strong specificity, can exclude fluorescent interference in environmental matrix, and is used for lipopolysaccharide analysis and detection by combining the colorimetric analysis of color picker of smart phone, so that in-vitro naked-eye visible rapid quantitative detection of lipopolysaccharide can be realized, to meet the demand of on-site monitoring.

The invention relates to the technical field of water quality detection, and discloses a water quality fluoride detection method based on digital image colorimetric analysis, which comprises the following steps: constructing a zirconium-based nano-enzyme probe, and realizing fluorine ion specific recognition through Zr-Mn bimetal coordination and boric acid modification; developing a three-layer paper-based micro-fluidic chip, a fixed zirconium-based nano-enzyme probe, an integrated optical reference array and a distributed temperature control network; multiband light source switching is cooperatively controlled through the smart phone, and reflection and fluorescence bimodal images are synchronously collected; multi-color space features are extracted, and environment parameters are fused to construct a dynamic compensation matrix to correct matrix interference; the corrected features are processed, and the fluoride concentration and the confidence interval are output; and the detection data is encrypted and then written into the block chain, and data verification and standard exceeding alarm are realized through the smart contract. The device and the method have the advantages of simplicity and convenience in operation, low cost, high portability and on-site rapid detection, and are suitable for on-site rapid detection of fluorides in various water qualities.

The application discloses a nano-enzyme with peroxidase-like activity and a preparation method and application thereof. The nano-enzyme has characteristic peaks of Bi2O3 in an XRD pattern; and the nano-enzyme has characteristic peaks of Fe, C, Bi and O in an XPS full spectrum. The preparation method of the nano-enzyme comprises the following steps: (1) preparing FeBiMOFs by a solvothermal method; and (2) performing thermal activation treatment on the FeBiMOFs, so that the nano-enzyme is obtained. The preparation method is simple and easy to control. The heteroatom-doped Bi2O3-based nano-enzyme has great potential when used as a peroxidase mimic to detect organic phosphorus pesticides, and the application of Bi2O3 in the sensing field is expanded. The heteroatom-doped Bi2O3-based nano-enzyme is formed by accumulation of nanoparticles with a particle size of less than 10 nm, has a large specific surface area and multiple active sites for synergistic action. When applied to a colorimetric analysis method to detect AChE and organic phosphorus pesticides (OPs) such as DDVP, the nano-enzyme has the advantages of low detection limit, wide linear range and strong anti-interference ability, and has strong practicability.

A method of testing a saliva sample includes: providing a testing device having a color palette and multiple test areas, using colorimetric analysis and lateral flow assay tests; collecting at least a prescribed volume of a saliva sample; dispensing, onto each of multiple test areas of a testing device, a respective portion of the collected saliva sample, the testing device thereby developing a respective discernible reaction at each test area; capturing, by a computing device, an image of testing device; analyzing, through a testing platform, the image by colorimetric and liquid flow assay analysis; and displaying results of the colorimetric analysis, the displayed results representing oral health parameters. The displayed results including quantitative indices and trend identifiers related to oral health parameters. A kit for use in conducting the above method includes the sample reactive device.

The utility model discloses equipment for measuring total phosphate in boiler water and cooling water, and belongs to the technical field of devices for measuring phosphate. A device for measuring total phosphate in boiler water and cooling water comprises a pipette, a colorimetric tube, a spectrophotometer, filter paper and a beaker, and further comprises a water bath kettle used for heating the colorimetric tube; the placing groove is formed in the water bath kettle, and the placing groove is used for placing a colorimetric tube; the plurality of groups of hollowed-out hole baskets are arranged in the placing groove; a filtered sample is directly added into the colorimetric tube, is digested for half an hour in a water bath environment, and then is cooled for colorimetric, so that the risks of breakage and incomplete transfer caused by directly heating a flask with open fire are effectively avoided, the accuracy of an analysis result is ensured, and the threaded column and the jacking ring can be matched with a screw rod, so that the detection accuracy is improved. Furthermore, the position of the colorimetric tube is adjusted, so that the colorimetric tubes with different lengths can be conveniently heated.

The application provides a peritoneal dialysis liquid detection data intelligent analysis method based on visual recognition, which comprises the following steps: acquiring a test paper image to be recognized, and performing test paper region segmentation, color correction and test paper detection region brightness equalization processing on the test paper image to obtain a processed test paper detection region; acquiring a standardized color feature vector of a test paper color developing region from the test paper detection region; mapping and matching the standardized color feature vector with a standard colorimetric database to obtain a colorimetric matching value, performing color abnormality degree recognition according to the colorimetric matching value, obtaining a test paper colorimetric analysis result according to the color abnormality degree and a preset abnormal value, and performing self-adaptive correction on the test paper colorimetric analysis result with an abnormality; and constructing a personalized colorimetric model according to the colorimetric matching value combined with a historical colorimetric matching value to perform health trend analysis and early warning.

The utility model belongs to the technical field of colorimetric analysis devices, and particularly discloses a universal analysis device for a rotary cuvette and a colorimetric tube, which comprises a motor fixing plate, a rotary disc base and an analysis groove, the rotary disc base is arranged above the motor fixing plate, a rotary disc fixing hole is arranged in the middle of the rotary disc base, and the rotary disc fixing hole is communicated with the analysis groove. A stepping motor is arranged below the motor fixing plate, a driving shaft of the stepping motor is connected with a motor bearing, the motor bearing penetrates through the rotating disc fixing hole and is connected with a cylindrical fixing piece, and the rotating disc base is connected with the motor bearing through the cylindrical fixing piece. A plurality of analysis grooves are uniformly formed above the rotating disc base, light holes are formed in the side edges of the analysis grooves, and stainless steel elastic pieces are arranged in the analysis grooves; the cuvette or the colorimetric tube is inserted into the analysis tank, and the stainless steel elastic sheet is used for extruding and limiting the cuvette or the colorimetric tube; the stepping motor drives the rotating disc base to rotate, and the positions of different analysis grooves are changed.

The present invention relates to colorimetric analysis and detection technology, and discloses a method for detecting lead ions based on the CIRSPR-Cas system. The method comprises the following steps: mixing a specific enzyme containing a target DNA fragment with a sample to be tested that may contain lead ions to form a first mixture to be tested; mixing the first mixture to be tested with a CRISPR-Cas12a system solution to carry out a mixing reaction to obtain a second mixture to be tested; mixing the second mixture to be tested with a biosensor containing single-stranded DNA to carry out a mixing reaction to obtain a reaction solution to be tested, mixing the precipitate obtained by solid-liquid separation of the reaction solution to be tested with a color-developing agent to carry out a color-developing reaction to obtain a color-developing reaction solution, and carrying out colorimetric analysis or absorbance value detection analysis on the color-developing reaction solution; wherein, the target DNA can activate the Cas12a protein in the CRISPR-Cas12a system. The method has high detection sensitivity for heavy metals, can effectively shorten the detection time, and has strong practicability.

Reaction of bleach with a soluble bromide salt to produce brightly colored hypobromite ion enables easy colorimetric measurement of active chlorine, or NaOCl content of bleach. Water of dilution and a suitable buffer are combined with bleach to provide a stable color over a wide range of bleach composition. This analytical method is well suited for use outside the laboratory at sites where bleach is stored or used; for example, but not limited to industrial sites that use bleach, places where bleach is used to destroy microbes and / or hazardous substances in water, and commercial laundries. In addition to analyzing bleach itself, process water streams containing some amount of bleach or another source of active chlorine can be analyzed over a wide range of NaOCl concentration.

A calibration method and related systems for colorimetric chemical assay and a fluid sensor apparatus using the same are provided. Reference measurements are taken from both a digital camera and a spectrometer. The digital camera is installed in the fluid sensor apparatus. Subsequently, color measurements from the digital camera are then converted and compared to prior spectrometric measurements to perform a colorimetric analysis.

A blood testing device for detecting hemolysis in a blood sample is described. The blood testing device comprises an housing for containing the blood sample. The housing has a treatment window and an optical zone formed therein. The blood testing device further includes an acoustic transducer positioned to selectively generate acoustic forces directed into the treatment window of the housing and a control unit for selectively actuating and deactuating the acoustic transducer to permit colorimetric analysis of plasma within the blood sample.

The application relates to the technical field of water quality detection, and discloses a water quality fluoride detection method based on digital image colorimetric analysis, which comprises the following steps: constructing a zirconium-based nanoscale enzyme probe, realizing specific recognition of fluorine ions through Zr-Mn bimetal coordination and boric acid modification; developing a three-layer paper-based microfluidic chip, fixing the zirconium-based nanoscale enzyme probe, integrating an optical reference array and a distributed temperature control network; synchronously collecting double-mode images of reflection and fluorescence through intelligent mobile phone collaborative control of multi-band light source switching; extracting multi-color space features, fusing environmental parameters to construct a dynamic compensation matrix to correct matrix interference; processing the corrected features to output fluoride concentration and a confidence interval; and writing the detection data into a block chain after encryption, and realizing data verification and over-limit alarm through an intelligent contract. The application has the advantages of simple operation, low cost, strong portability and on-site rapid detection, and is suitable for on-site rapid detection of various water quality fluorides.

The present application relates to the field of agricultural biotechnology and plant science technology, and discloses a winter melon seedling salt tolerance identification method based on multi-modal characteristics, which comprises the following steps: uniformly germinating winter melon seeds, selecting seedlings with consistent growth vigor, setting up a clear water control group and a salt stress group with a response concentration of NaCl, and collecting multi-modal data of photosynthetic fluorescence, photosynthetic absorption, colorimetric analysis and morphology at a preset time point. After the multi-modal data is standardized and normalized, it is reduced in dimension by principal component analysis, and the principal component scores are dimensionless by the membership function method. The weight is calculated by combining the contribution rate, and the comprehensive salt tolerance evaluation value is obtained by weighting. According to the comprehensive salt tolerance evaluation value, the mean value and the standard deviation, the salt tolerance is divided into six levels: normal, high resistance, medium resistance, medium sensitivity, sensitivity and high sensitivity, so that scientific identification is realized, and support is provided for winter melon salt-tolerant germplasm screening and variety breeding.

The invention discloses a composite driving system based on combination of a surfactant and cyclodextrin and application of the composite driving system. The nucleic acid aggregate is formed by electrostatic adsorption and hydrophobic interaction between the cationic surface active agent and nucleic acid molecules, so that efficient enrichment of nucleic acid is realized; separation of nucleic acid is realized by separating the aggregate and the supernatant of the solution; and then cyclodextrin is added, and the charge neutralization effect of the surfactant on nucleic acid is weakened by utilizing the inclusion effect of a hydrophobic cavity of cyclodextrin on an alkyl chain of the surfactant, so that the nucleic acid aggregate is controllably released. The composite system can realize reversible enrichment of nucleic acid, is simple and convenient to operate, can be compatible with various detection means (including fluorescence, electrochemistry and colorimetric analysis), and is particularly suitable for a miniaturized and on-site nucleic acid detection platform. The invention provides a dynamic controllable nucleic acid enrichment strategy, and provides a new technical approach for rapid and sensitive molecular detection.

Pathology Laboratory Equipment for Colorimetric Analysis of Urine Test Strips

The present application belongs to the field of analytical chemistry and electrochemistry, and particularly relates to an electrochemical sensor, a preparation method and application thereof in detection of sweat triglyceride content. A wearable sensor is prepared based on C-ZIF-67 / KB nanocomposite material, the sensor realizes detection of sweat triglyceride for the first time, and it is verified that sweat triglyceride level has high correlation with blood triglyceride level (correlation coefficient is 0.813, n=35). Through verification of a commercial colorimetric analysis kit, the sensor obtains a high correlation coefficient of 0.853 in detection of sweat triglyceride, which shows excellent accuracy.

The present invention discloses an application of a bifunctional platinum-modified nickel-iron layered double metal hydroxide in the quantitative and qualitative detection of polyphenols. The present invention prepares Pt / NiFe‑LDH, which can be used as a nanozyme material for colorimetric analysis and a matrix for LDI MS. Among them, the abundant hydroxyl groups on LDH can selectively capture hydroxyl-containing molecules such as polyphenols, and Pt / NiFe‑LDH exhibits high peroxidase activity, which can catalyze the decomposition of hydrogen peroxide to oxidize 3,3',5,5'‑tetramethylbenzidine to blue, and the antioxidant properties of polyphenols can affect the color depth of the system, and the linear relationship between the absorbance under the detection signal and the polyphenol content in the system can be used to achieve polyphenol quantification. On the other hand, Pt / NiFe‑LDH-assisted LDI MS has good signal intensity feedback in the monomer detection of polyphenols, and can monitor the oxidation process of polyphenols by virtue of a short detection time interval (a few seconds).

The invention belongs to the technical field of gas chemical sensing and fluorescence / colorimetric analysis, and particularly discloses a preparation method and application of carbon dots for detecting carbon monoxide. The preparation method of the carbon dots for detecting carbon monoxide comprises the following steps: dissolving amino acid and copper salt in water to obtain a mixed solution; placing the mixed solution in a high-pressure reactor for heating reaction to obtain a carbon dot crude product; and purifying the carbon dot crude product to obtain the carbon dot for detecting carbon monoxide. The carbon dots prepared by the method have the advantages of simplicity in preparation, abundant surface functional groups, uniform particle size distribution, good water solubility and stability and the like, and rapid fluorescence detection of CO is realized under mild conditions; and the characteristic has a specific fluorescence and colorimetric bimodal response effect on CO, and qualitative and semi-quantitative detection of CO can be realized.

The invention discloses a preparation method of a cobalt-doped prussian blue analogue (Co-PBA) nano-enzyme for detecting DA (diphenylamine) and TYR (triphenylamine). The Co-PBA nano-enzyme shows excellent laccase-like activity, and can efficiently catalyze DA oxidation to generate dopaquinone; then, the dopaquinone and a chromogenic substrate 4-aminoantipyrine (4-AP) are subjected to a specific condensation reaction, a pink product N-(1-phenyl-3-methyl-5-pyrazolone)-dopaquinone imine with a pi-pi conjugated structure is formed, and signal transduction is achieved. Furthermore, through a cascade reaction that TYR catalyzes tyramine hydrochloride to oxidize to generate DA, the detection system is successfully expanded to TYR activity analysis. The Co-PBA nano-enzyme sensing platform has the advantages of simplicity and convenience in operation and visual reading, and provides a new strategy for on-site rapid detection of DA and TYR in combination with a smartphone colorimetric analysis technology.

This invention relates to the field of electrochemical-colorimetric analysis and detection technology, specifically to a bifunctional Ru / MoB MBene sensing probe, its preparation method, sensor, and applications. The key technical points are: mixing surface-modified Ru with a two-dimensional MoB MBene nanosheet suspension, and preparing a Ru / MoB MBene composite material via electrostatic self-assembly as a bifunctional sensing probe. This probe is used to construct an electrochemical-colorimetric dual-mode sensor, employing a dual-mode signal output strategy to fully leverage the advantages of electrochemical methods in terms of sensitivity, linear range, and precise quantification. It also integrates rapid colorimetric visualization and on-site screening capabilities. The two modes can operate independently or mutually verify each other, significantly improving the reliability of detection results, resistance to matrix interference, and practical applicability. This overcomes the problems of poor portability, long detection cycles, and insufficient sensitivity to trace micro / nanoplastics found in traditional equipment.

This invention discloses a method for identifying photomask defects, belonging to the field of photomask inspection technology. The method includes: S1, acquiring a surface defect image; S2, preprocessing the surface defect image based on the imaging characteristics of the defect; S3, correcting the preprocessed surface defect image using a filtering algorithm; S4, performing colorimetric analysis on the corrected surface defect image to obtain particle regions; S5, segmenting the particle regions based on the morphological features of the defect to obtain defect islands; S6, performing morphological analysis on the defect islands to obtain the defect type and size. The photomask defect identification method provided by this invention automatically processes surface defect images and extracts defect islands by combining morphological and colorimetric analysis methods, improving the accuracy of defect identification, overcoming the limitations of differences in imaging systems across different devices, achieving automatic batch processing, and saving manpower and material resources in mass production.

The preparation method comprises the following steps: mixing a zinc salt aqueous solution with a 2-methylimidazole aqueous solution to prepare ZIF-8 nano-particles, dispersing the ZIF-8 nano-particles into an alcoholic solution to obtain a ZIF-8 alcoholic solution, mixing the ZIF-8 alcoholic solution with a polyphenol aqueous solution, and carrying out colorimetric analysis on the ZIF-8 alcoholic solution to obtain the manganese-based nano-enzyme. The preparation method comprises the following steps: preparing polyphenol cross-linked ZIF-8 nano-particles, dispersing the polyphenol cross-linked ZIF-8 nano-particles into water to obtain a polyphenol cross-linked ZIF-8 aqueous solution, and mixing the polyphenol cross-linked ZIF-8 aqueous solution with a potassium permanganate aqueous solution to obtain the manganese-based nano-enzyme. The method comprises the following steps: mixing 2, 4-dichlorophenol, 4-aminoantipyrine and manganese-based nano-enzyme, adding a buffer solution to obtain a mixed solution, mixing a tobacco shred leachate with the mixed solution to obtain a reaction solution, and rapidly detecting glyphosate in tobacco shreds by observing the color change of a reaction system and the change of an ultraviolet absorbance value. The method saves a lot of time for detecting the glyphosate in the tobacco shreds.