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Cold-adapted cellulase-producing bacillus subtilis and screening method thereof

A technology of Bacillus subtilis and cellulase, which is applied in the field of microbiology, can solve the problems that the screening method of Bacillus subtilis has not been reported yet, and achieve the effects of improving the micro-ecosystem, enhancing air permeability, and increasing the emergence rate of seeds

Active Publication Date: 2019-12-10
LIAONING ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In conclusion, a low-temperature cellulase-producing Bacillus subtilis and its screening method have not been reported

Method used

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  • Cold-adapted cellulase-producing bacillus subtilis and screening method thereof
  • Cold-adapted cellulase-producing bacillus subtilis and screening method thereof
  • Cold-adapted cellulase-producing bacillus subtilis and screening method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment example

[0066] CK: 1 ton of corn stover adjusts the water content to 50%.

[0067] TY-1: Add bacterial agent TY-16kg on the basis of CK.

[0068] Composting time: 2019.3.13-2019.4.25.

[0069] Compost location: Shenyang.

[0070] Specific process: For each pile of 1000kg of compost, pile the corn stalks neatly parallel to the ground. When every 20cm rises, sprinkle a small amount of inoculant on the surface of the compost. At the same time, step on it tightly, keep the pile height 1.5, and cover the outer surface with agricultural plastic film , A total of 45d.

[0071] Indicators: Determine the temperature of straw composting and the lignin and cellulose content of straw in different periods.

[0072] The results showed that: under the condition of spring average temperature of 6℃, by adding low-temperature cellulase-producing Bacillus subtilis, the average temperature of straw compost reached 47.3℃, which was 50.7% higher than the control, lignin was 31.5% lower than the control, and the cell...

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Abstract

The invention relates to cold-adapted cellulase-producing bacillus subtilis and a screening method thereof. The cold-adapted cellulase-producing bacillus subtilis comprises bacillus subtilis with a preservation mechanism being General Microbiological Culture Center for China Committee for Culture Collection of Microorganisms, a preservation number being CGMCC No. 18001 and a preservation date being June 19, 2019. For the cold-adapted cellulase-producing bacillus subtilis and the screening method, efficient cold-adapted cellulase bacillus subtilis is separated and screened from soil in different low-temperature areas, and the biological characteristics of the bacillus subtilis are as follows: a bacterial colony on an LB culture medium is relatively small, round, wet, smooth and sticky, is easy to pick and has typical bacterial colony characteristics; and in addition, the influences of the conditions, including temperature, carbon sources, nitrogen sources and a pH value, of the bacterial colony on cellulase are studied. By applying the cold-adapted cellulase-producing bacillus subtilis to straw returning in northeast low-temperature cold areas, the influence of straw incineration inthe northeast areas on environment is reduced, and a solid foundation is laid for sustainable development of agriculture.

Description

Technical field [0001] The invention belongs to the field of microbiology, in particular to a low-temperature cellulase-producing Bacillus subtilis and a screening method thereof. Background technique [0002] Low-temperature microorganisms refer to groups of microorganisms that have the ability to grow and reproduce in a low-temperature environment, and they grow and reproduce in various low-temperature environments. According to the selection of the growth temperature of microorganisms, low-temperature microorganisms are divided into two types, psychrophilic microorganisms and cold-tolerant microorganisms. Among them, psychrophilic microorganisms can grow and reproduce, and the optimum temperature is about 15℃, which can survive. The temperature is about 20℃; cold-tolerant microorganisms are microorganisms whose survival temperature is about 0℃ and the optimum temperature for growth and reproduction is about 20℃. There are very rich microbial resources and low-temperature micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C12Q1/34C12Q1/04C12R1/125
CPCC12N9/2437C12Y302/01004C12Q1/34C12Q1/04G01N2333/942C12R2001/125C12N1/205
Inventor 何志刚隋国民孙文涛刘惠屿刘艳
Owner LIAONING ACAD OF AGRI SCI
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