Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer

A technology of compound microbial agent and water-soluble fertilizer, which is applied in the direction of microorganisms, urea compound fertilizers, and methods based on microorganisms, can solve problems such as affecting soil fertility, crop toxicity, and soil microbial flora imbalance, and achieve the prevention of cropping diseases , inhibit growth, promote growth effect

Inactive Publication Date: 2016-04-20
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, when crops absorb nutrients from the soil to grow, they excrete some self-generated metabolic wastes, which will also cause toxicity to th

Method used

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  • Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer
  • Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer
  • Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The composite microbial bacterial agent and the composite microbial water-soluble fertilizer of the present invention are prepared according to the following steps.

[0032] 1) Inoculate Bacillus subtilis, low-temperature bacteria VT16, and low-temperature bacteria VT19 in liquid NA medium, yeast inoculate in liquid PDA medium, and culture the yeast on a shaker at 200 r / min for 2 days at 15°C. Bacillus subtilis was cultured on a 100r / min shaker at 15°C for 1 day, and the low-temperature bacteria VT16 and VT19 were cultured on a 100r / min shaker at 15°C for 1 day. The OD600 value of single strain liquid culture reached 2.0, Produce first-class seeds;

[0033] PDA medium formula:

[0034] Potatoes 200g, sucrose 20g, agar 20g, water 1000mL, pH natural, sterilized at 121°C for 20min.

[0035] NA medium formula:

[0036] Beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH7.0, sterilized at 121°C for 20min.

[0037] 2) Secondary seed cultivation: The volume ra...

Embodiment 2

[0047] The composite microbial bacterial agent and the composite microbial water-soluble fertilizer of the present invention are prepared according to the following steps.

[0048] 1) Inoculate Bacillus subtilis, low-temperature bacteria VT16, and low-temperature bacteria VT19 in liquid NA medium, inoculate yeast in liquid PDA medium, and culture the yeast on a shaker at 200 r / min for 6 days at 38°C. Bacillus subtilis Bacillus was cultured on a shaker at 200r / min for 3 days at 42°C, low-temperature bacteria VT16 and VT19 were cultured on a shaker at 200r / min at 25°C for 3 days, and the OD600 value of single strain liquid culture reached 4.0. primary seed;

[0049] PDA medium formula:

[0050] Potatoes 200g, sucrose 20g, agar 15g, water 1000mL, pH natural, sterilized at 121°C for 20min.

[0051] NA medium formula:

[0052] Beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH7.0, sterilized at 121°C for 20min.

[0053] 2) Secondary seed cultivation: The volume r...

Embodiment 3

[0064] The composite microbial bacterial agent and the composite microbial water-soluble fertilizer of the present invention are prepared according to the following steps.

[0065] 1) Inoculate Bacillus subtilis, low-temperature bacteria VT16, and low-temperature bacteria VT19 in liquid NA medium, yeast inoculate in liquid PDA medium, and culture the yeast on a shaker at 150 r / min for 4 days at 30°C, and Bacillus subtilis The bacilli were cultured on a shaker at 150r / min for 2 days at 37°C, and the low-temperature bacteria VT16 and VT19 were cultured on a shaker at 150r / min at 20°C for 2 days. primary seed;

[0066] PDA medium formula:

[0067] Potatoes 200g, sucrose 20g, agar 15g, water 1000mL, pH natural, sterilized at 121°C for 20min.

[0068] NA medium formula:

[0069] Beef extract 3g, peptone 10g, NaCl 5g, agar 15g, water 1000mL, pH 7.1, sterilized at 121°C for 20min.

[0070] The difference between solid medium and liquid medium is that a proper amount of agar is ad...

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PUM

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Abstract

The invention discloses a compound microbial agent, a water-soluble fertilizer containing the same and applications of the compound microbial agent and the water-soluble fertilizer. The compound microbial agent is a mixture of high organic matter yeast fermentation liquid and traditional Chinese medicine extraction waste liquid, wherein the high organic matter yeast fermentation liquid is produced through microbial fermentation and is rich in amino acid. A preparation method is simple, dispenses with aseptic conditions and is low in cost. The compound microbial agent is prepared through mixed inoculated fermentation by taking bacillus subtilis, saccharomycetes, a cold-adapted bacterium VT16 and a cold-adapted bacterium VT19 as a composite strain and utilizing the high organic matter liquid produced through fermentation and rich in amino acid and the traditional Chinese medicine extraction waste liquid as raw materials, is comprehensive in nutrients and can not only provide nutrient elements required by crop growth but also inhibit reproduction of harmful microbes. The compound microbial agent is then combined with nutrients N, P and K essential to crops, wherein the added nutrients can meet the requirements of plant growth and have high absorptivity and good absorption effects; the added microbes can conduce to inhibiting growth of plant pathogens, preventing continuous cropping diseases, promoting growth of crops and inducing plants to become resistant.

Description

technical field [0001] The invention relates to the technical field of agricultural fertilizers, in particular to a composite microbial agent, a water-soluble fertilizer containing the composite microbial agent and applications thereof. Background technique [0002] Water-soluble fertilizer is a multi-component compound fertilizer that can be quickly and completely dissolved in water. The advantages of water-soluble fertilizer are fast dissolution, no residue, and high absorption and utilization rate of crops. The research on water-soluble fertilizers abroad is earlier. At present, in some industrially developed countries, water-soluble fertilizers have been widely used to irrigate vegetables, fruit trees, flowers planted in greenhouses, lawns of golf courses, landscaping plants, etc., and some even Used to maintain home green plants. [0003] Compared with traditional calcium superphosphate and granulated compound fertilizers, water-soluble fertilizers have obvious advanta...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05G3/00C05G3/04C12R1/125C05G3/80
CPCC05B7/00C05G3/00C05G3/80C12N1/16C12N1/20C05C9/00C05F11/02
Inventor 任莉
Owner 北京沃土天地生物科技股份有限公司
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