Antarctic cold-adapted microbial extracellular polysaccharide capable of improving body immunity

A technology of extracellular polysaccharides and immunity, applied in the field of exopolysaccharides of cold-adapted microorganisms in Antarctica

Active Publication Date: 2011-09-07
博德生物技术(德州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The fermented liquid of Antarctic cold-adapted bacteria Pseudoal termonas sp.S-5 can be applied to the cold resistance of plants and crops, such as the Chinese patent with publication number CN101775361A (application number 200910256034.6), in which the antifreeze active substance is Antarctic cold-adapted The exopolysaccharide produced by the bacterium Pseudoaltermonas sp.S-5 has a molecular weight of about 39,7046Da, but the efficacy of this exopolysaccharide in improving the body's immunity has not been reported

Method used

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  • Antarctic cold-adapted microbial extracellular polysaccharide capable of improving body immunity
  • Antarctic cold-adapted microbial extracellular polysaccharide capable of improving body immunity
  • Antarctic cold-adapted microbial extracellular polysaccharide capable of improving body immunity

Examples

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Effect test

Embodiment 1

[0032] A method for preparing an exopolysaccharide of cold-adapted microorganisms in Antarctica capable of improving body immunity, comprising the steps of:

[0033] (1) After activating the Antarctic cold-adapted bacterium (Pseudoaltermonnas sp.) S-5 with the strain preservation number CGMCC NO.3433, inoculate it in Zobell 2216E liquid medium, cultivate it at 9°C for 24 hours, and inoculate it at 1 wt%. Zobell 2216E liquid medium is used to expand the culture, and the bacteria can be multiplied;

[0034] (2) by the inoculum amount of 10wt% by the multiplication bacterial classification that step (1) makes, be inoculated in the fermentation medium in the liquid fermentation tank, after adding the sterilized soybean oil of fermentation medium weight 0.3% in the fermentation medium , 7-9°C aerobic stirring culture for 50-60h, stirring speed 160-170r / min, to obtain a liquid fermentation broth;

[0035] (3) Centrifuge the liquid fermentation broth prepared in step (2) for 15min a...

Embodiment 2

[0045] Example 2 Effect of in vitro administration of exopolysaccharides from cold-adapted microorganisms on the active proliferation of mouse spleen lymphocytes

[0046] The mice were sacrificed by cervical dislocation, and the spleen was taken aseptically to prepare splenic lymphocytes, which were made into 1×10 6 / ml cell suspension. Mouse 1 x 10 6 / ml splenocyte suspension was added to a 96-well cell culture plate, 100 μl per well, and the experiment was set up as a control group, exopolysaccharide (final concentrations were 0.1, 0.25, 0.5, 1, 2.5, 5, 10 μg / ml) stimulation group, For ConA (final concentration: 5 μg / ml) stimulation group, six replicate wells were set up for each concentration in each group, and the blank control group was replaced by an equal volume of RPMI 1640. At 37°C, 5% CO 2 Cultivate for 72 hours in a saturated humidity incubator. 10 μl of MTT solution was added to each well 4 hours before the end of the culture, and the culture was continued unti...

Embodiment 3

[0052] Example 3: Influence of in vitro administration of exopolysaccharides from cold-adapted microorganisms on the activity of acid phosphatase in mouse macrophages

[0053] Mice were intraperitoneally injected with 1ml of 4% starch solution, and sacrificed by cervical dislocation 3 days later, aseptically collected peritoneal macrophages of mice to prepare cell suspension; the peritoneal cell suspension was added to a 96-well cell culture plate, 100 μl per well. At 37°C, 5% CO 2 CO 2 Incubate for 4 hours in the incubator. Remove the supernatant, and the adherent cells are macrophages. The experiment set up control group, exopolysaccharide (0.1, 0.25, 0.5, 1, 2.5, 5, 10 μg / ml final concentration) stimulation group, LPS (1 μg / ml final concentration) stimulation group, 100 μl per hole, each group Six replicate wells were set up for each concentration. At 37°C, 5% CO 2 CO 2 Incubate for 24 hours in the incubator. Take it out, remove the supernatant, add 100 μl of pNPP so...

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Abstract

The invention relates to an Antarctic cold-adapted microbial extracellular polysaccharide capable of improving body immunity, and belongs to the technical field of medicaments and health-care food. The cold-adapted microbial extracellular polysaccharide is prepared by culturing, fermenting, separating and purifying Antarctic cold-adapted bacteria (Pseudoaltermonas sp.) S-5, the strain collection number of which is CGMCC NO.3433. The molecular weight of the polysaccharide is 39,7046Da, and the polysaccharide consists of mannose, dextrose and galactose in a molar ratio of 4.8: 50.9: 44.3. The prepared Antarctic cold-adapted microbial extracellular polysaccharide capable of improving the body immunity can promote the proliferation of in vitro spleen lymphocytes, enhance the activity of macrophages and improve the body immunity.

Description

technical field [0001] The invention relates to an exopolysaccharide of Antarctic cold-adapted microorganisms capable of improving body immunity, and belongs to the technical field of medicine and health food. Background technique [0002] The Antarctic ocean is covered with ice and snow all year round, and the geographical environment and climate characteristics are very special. The microorganisms living in this extreme environment have a high degree of diversity. This diversity provides abundant resources for the development of active substances with special application value. The exopolysaccharide produced by Antarctic microorganisms can alleviate the damage of low temperature and high salt to the bacteria, and is a good antifreeze protectant, which plays an important role in low temperature protection for microorganisms to adapt to the harsh environment of Antarctic low temperature and high salt. The exopolysaccharides of Antarctic microorganisms have novel structures a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/736A61P37/04A23L1/30C12R1/01
Inventor 陈靠山白云贵张鹏英陈国创禹文茜
Owner 博德生物技术(德州)有限公司
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