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LDL-T strain as attenuated vaccine strain for avian infectious bronchitis and application thereof

A bronchitis and chicken infectious technology, applied in the field of prevention and treatment of chicken infectious bronchitis, can solve the problems of unsatisfactory IB vaccine control effect and inability to effectively prevent the attack of popular strains of bronchitis virus, and achieve good immunity Protective, good protective effect, good safety effect

Active Publication Date: 2017-07-14
HARBIN WEIKE BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main technical problem to be solved by the present invention is to overcome the defects that the existing vaccine strains cannot effectively prevent the attack of TW I type chicken infectious bronchitis virus epidemic strains, and the current existing IB vaccines have unsatisfactory control effects, and provide a A new chicken infectious bronchitis attenuated vaccine strain, the attenuated vaccine strain can effectively resist the attack of TW type I infectious bronchitis virus, and can provide good immune protection effect

Method used

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  • LDL-T strain as attenuated vaccine strain for avian infectious bronchitis and application thereof
  • LDL-T strain as attenuated vaccine strain for avian infectious bronchitis and application thereof
  • LDL-T strain as attenuated vaccine strain for avian infectious bronchitis and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The cultivation and identification of chicken infectious bronchitis attenuated vaccine strain LDL-T strain

[0026] 1 Materials and methods

[0027] 1.1 Gradient cold adaptation of virus strain ck / CH / LDL / 140520 weakened

[0028]In order to ensure that infectious bronchitis virus can rapidly and stably attenuate the virus through the continuous passage of chicken embryos, 9-10 days old SPF chicken embryos were used to conduct continuous gradient cooling on the strong IBV strain ck / CH / LDL / 140520 Cold-adapted subculture. During the subculture process, 5 chicken embryos were inoculated through the allantoic cavity of chicken embryos, and each embryo was inoculated with 0.1ml. Incubate for 72 hours. After 72 hours, take out the chicken embryos and place them at 2-8°C. After cooling for 18-24 hours, observe the lesions of the chicken embryos, collect the allantoic fluid of the chicken embryos aseptically, and carry out the next passage. Incubation temperature t...

Embodiment 2

[0061] Example 2 Gradient cold-adapted attenuated vaccine strain LDL-T strain immune efficacy and protective effect experiment

[0062] 1. Experimental method

[0063] Forty 1-day-old SPF chickens were randomly divided into 2 groups (20 in each group), and were raised in a negative pressure isolator, and the chicks had free access to food and water. At the age of 5 days, one group of chicks received LDL-T (P75, 10 3 EID 50) for inoculation with 100 μL intranasally; another group of chicks was used as a control group with 100 μL normal allantoic fluid intranasally. From the date of inoculation, observe and record the morbidity and mortality of the vaccinated flock every day, and 2 groups of chicks are simultaneously treated with homologous virulent ck / CH / LDL / 140520P2 (10 days) 20 days after immunization. 6 EID 50 / 0.1ml) for nasal and eye drop challenge, 100 μL for each bird; 5 days after the challenge, each chicken took a throat swab to isolate the virus respectively, and ...

Embodiment 3

[0075] The preparation of embodiment 3 TW I type chicken infectious bronchitis attenuated vaccine

[0076] Take the attenuated vaccine strain (LDL-T strain), restore the culture condition to 37°C, proliferate the virus seeds for production, dilute 100,000 times with sterilized PBS, inoculate the allantoic cavity of 9-day-old SPF chicken embryos, and inoculate 0.1ml per embryo. After inoculation, the pinholes were sealed and incubated at 37°C. The chicken embryos were inoculated for 24 hours and the embryos were illuminated, the dead embryos were discarded, and the air chamber of the chicken embryos incubated at 37°C for 36-72 hours was upward, and placed at 4°C to cool for 24 hours. Harvest the virus allantoic fluid, mix several chicken embryo allantoic fluids into a group, put them in a sterilized bottle, store them at 2-8°C, and do a sterility test at the same time, and the virus content should be ≥ 10 6.0 EID 50 / 0.1ml.

[0077] The virus allantoic fluid that has passed ...

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Abstract

The invention discloses an LDL-T strain as an attenuated vaccine strain for avian infectious bronchitis and application thereof, which belong to the field of prevention and treatment of the avian infectious bronchitis. The attenuated live vaccine strain CGMCC No.12549 for the avian infectious bronchitis provided by the invention has good safety and immune protection, the immune protection rate reaches 80 percent or more, and the LDL-T strain has good protective efficacy on TW I type avian infectious bronchitis viruses, and can effectively protect chickens against the attack of the TW I type infectious bronchitis viruses with strong virulent; moreover, the LDL-T strain is safe for chickens and laying breeders, side reaction does not exist, and the safety is good. The cold-adapted attenuated vaccine strain disclosed by the invention can be applied to the preparation of single vaccines, mixed vaccines and the like for preventing and treating the avian infectious bronchitis viruses.

Description

technical field [0001] The invention relates to attenuated chicken infectious bronchitis vaccine strain LDL-T strain and application thereof, belonging to the field of prevention and treatment of chicken infectious bronchitis. Background technique [0002] Avian Infectious Bronchitis (IB) is an acute, highly contagious, viral respiratory disease caused by Avian Infectious Bronchitis Virus (IBV). There are huge differences depending on the type. The "respiratory lesion type" mainly manifests as coughing, sneezing, and tracheal rales in sick chickens; Large, pale, dilated ureters, a large amount of urate deposits, and the appearance of a typical "mottled kidney"; "reproductive system lesion type" in addition to the initial clinical symptoms of "respiratory lesion type", the adult hen after infection of chicks showed The reproductive system such as fallopian tube produces pathological change, and becomes " false hen ", causes heavy economic loss to poultry industry. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
CPCA61K39/12A61K2039/543A61K2039/552C12N7/00C12N2770/20021C12N2770/20034
Inventor 刘胜旺韩宗玺
Owner HARBIN WEIKE BIOTECH DEV
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