Vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof

A porcine circovirus and swine fever virus technology, applied in antiviral agents, virus antigen components, medical preparations containing active ingredients, etc., can solve the problems of cumbersome seed virus preparation process, reduced immune effect, and large batch-to-batch differences , to avoid immune stress response and reduce the immune effect, simplify the production process, and achieve the effect of high titer content

Active Publication Date: 2014-03-12
PU LIKE BIO ENG
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The preparation process of this method is cumbersome, the controllability is poor, the difference between batches is large, the production cycle is long, and it can only prevent and treat one disease
[0007] Therefore, the problem that exists at present is to need research and development a kind of pro

Method used

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  • Vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof
  • Vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof
  • Vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] 1) Preparation of swine fever virus antigen;

[0037] 2) Preparation of porcine circovirus type 2 subunit antigen;

[0038] 3) The ratio of swine fever virus antigen and porcine circovirus type 2 subunit antigen is mixed and lyophilized to prepare a dual vaccine composition against swine fever virus and porcine circovirus type 2 infection;

[0039] 4) The dual vaccine composition against swine fever virus and porcine circovirus type 2 infection and the two vaccines were separately immunized with animals to evaluate their efficacy.

[0040] Scheme 2 is to use the prepared porcine circovirus type 2 whole virus antigen inactivated vaccine as a diluent, dilute the swine fever virus vaccine to obtain a combination vaccine composition against swine fever virus and porcine circovirus type 2 infection The preparation of a dual vaccine composition kit against swine fever virus and porcine circovirus type 2 infection includes the following specific implementation steps:

[0041] 1) Prepar...

Example Embodiment

[0050] Example 1: Mixing swine fever virus and porcine circovirus type 2 subunit antigens and freeze-drying to prepare a dual vaccine composition against swine fever virus and porcine circovirus type 2 infection

[0051] 1. Preparation of swine fever virus antigen solution

[0052] (1) The ST cells (purchased from ATCC) (American type culture collection) that are highly sensitive to classical swine fever virus grown into a good monolayer were used 0.125wt% pancreatin and 0.03wt% EDTA ( Ethylenediaminetetraacetic acid) digestion solution was digested and dispersed, the cells were counted, and inoculated at an appropriate density, MEM containing 3wt%~5wt% FBS (fetal bovine serum) (purchased from PAA company, batch number A15110-1462) was added (Purchased from GIBCO company, batch number 856833), and at the same time add seed toxin according to MOI (multiplicity of infection)=0.1~0.2 ingestion dose, and place it in a 34~37℃ incubator for culture.

[0053] (2) Carry out the first collec...

Example Embodiment

[0089] Example 2: Using the prepared porcine circovirus type 2 whole virus antigen inactivated vaccine as a diluent to dilute the swine fever virus vaccine to obtain a combination vaccine composition against swine fever virus and porcine circovirus type 2 infection To prepare a dual vaccine composition kit against swine fever virus and porcine circovirus type 2 infection.

[0090] 1. Preparation of swine fever virus antigen solution

[0091] (1) Digest and disperse the ST cells (derived from ATCC) that have grown into a good monolayer and are highly sensitive to classical swine fever virus with a digestion solution of 0.125% trypsin and 0.03% EDTA. After counting the cells, they are inoculated at an appropriate density. , Add 3wt%~5wt% FBS (purchased from PAA company, batch number A15110-1462) MEM (purchased from GIBCO, batch number 856833), and at the same time add seed poison according to MOI=0.1~0.2 ingestion dose, place at 34~37℃ Cultivate in an incubator.

[0092] (2) Carry ou...

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Abstract

The invention relates to a bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof. According to the method, a porcine circovirus 2 subunit antigen and a swine fever virus antigen are mixed and lyophilized to prepare the bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection; or a porcine circovirus 2 totivirus antigen inactivated vaccine is used as a diluent to dilute a swine fever virus vaccine, so as to prepare a bivalent vaccine composition kit for resisting swine fever virus and porcine circovirus 2 infection. The bivalent vaccine composition does not produce immunity interference; after immunization, the swine fever virus antigen speeds up in immunoreaction; the protective antibodies provided by the vaccine composition reach an immunization protective dose one week earlier than a single vaccine antibody; and the antibodies produced by the porcine circovirus vaccine in the bivalent vaccine have similar effects as that produced by single vaccine immune. The method has the advantages of simple vaccine production process and high production efficiency. The animal experiments prove that the effect of the vaccine composition is better that that of single vaccine immune, therefore the invention has great application value.

Description

technical field [0001] The invention belongs to the technical field of animal biopharmaceuticals, and relates to a vaccine composition for pigs, in particular to a dual vaccine composition against classical swine fever virus and porcine circovirus type 2 infection and its preparation and application. Background technique [0002] Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV), which seriously endangers the swine industry. The mortality rate of this disease is as high as 80% to 90%. The World Organization for Animal Health (OIE) lists it as an OIE disease list, and my country also lists it as a first-class infectious disease. The prevention and control of swine fever has always been a subject that the countries with swine fever in the world attach great importance to. For this reason, all countries have invested a lot of manpower and material resources and achieved great results. Vaccination is still an important ...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/20A61K39/12A61K39/187
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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