531 results about "Animals experiments" patented technology

The invention relates to an antidepressant pharmaceutical composition and application thereof, and particularly discloses a pharmaceutical composition for treating depression as well as a preparation method and application thereof. The active pharmaceutical ingredients of the pharmaceutical composition are prepared from the following traditional Chinese medicines in parts by weight: 500 to 600 parts of radix bupleuri, 500 to 600 parts of fructus aurantii immaturus, 500 to 600 parts of radix aucklandiae, 500 to 600 parts of pericarpium citri reticulatae, 500 to 600 parts of rhizoma pinelliae preparata, 750 to 900 parts of herba taraxaci, 500 to 600 parts of scorched fructus crataegi, 300 to 360 parts of scorched semen arecae, 500 to 600 parts of paederia scandens, 300 to 400 parts of radix codonopsis, 300 to 400 parts of rhizoma corydalis and 500 to 600 parts of fried medicated leaven. The pharmaceutical composition has a remarkable curative effect on depression, animal experiments show that the pharmaceutical composition can remarkably enhance the social coefficient (showing that the pharmaceutical composition can improve social fear of depression patients) and improve sweet water preference (showing that the pharmaceutical composition is beneficial to transformation of negative emotions such as interest deficiency of the depression patients), and the pharmaceutical composition is safe to take, free of adverse reaction and toxic and side effects and free of toxic and side effects. And the composition can take effect quickly, and can take effect generally within 1-2 weeks after medication.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention relates to the technical field of microorganisms, and particularly discloses bifidobacterium adolescentis AGE17 capable of promoting growth and development of muscles and bones and application of the bifidobacterium adolescentis AGE17. The bifidobacterium adolescentis AGE17 is separated from excrement of healthy children and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33452, and the preservation date is January 17, 2025. The bifidobacterium adolescentis AGE17 has the advantages that the bifidobacterium adolescentis AGE17 can be used for preparing the biological organic fertilizer; animal experiments prove that the bifidobacterium adolescentis strain AGE17 provided by the invention has the function of promoting muscle and skeletal development, has more obvious and prominent effects compared with a model strain bifidobacterium adolescentis ATCC15703, and has important application value in increasing the muscle strength and height of children and adolescents.

The invention relates to the technical field of animal experiment equipment, and discloses an isolated culture device for mouse sweet water preference test in an IVC cage, which comprises a shell cover, the partition plate is fixedly installed on the inner side wall of the shell cover, and an inner cavity of the shell cover is divided into a living area and a functional area through the partition plate; the sliding cover is arranged in the living area of the shell cover and used for containing solid feed, and multiple sets of feeding trough openings which are arranged at equal intervals are formed in the bottom of the sliding cover; according to the device, an innovative cage-in-cage design is adopted, a single cage is converted into a plurality of independent experiment units on the premise that an existing IVC system is not changed, and cage position resources are greatly saved. The functions of isolation, double-bottle water drinking and independent feeding are integrated, and convenient operation is achieved through the pull-out type fixing plate and the sliding cover plate. The centrifugal tube is adopted to replace a special water bottle, so that the cost is reduced, the SPF environment is kept in the whole process, data accuracy and reliability are ensured, and the traditional SPT test problem is effectively solved.

The present invention relates to the technical field of the development and utilization of probiotics, specifically to a Weissella confusa DY12 with probiotic activity and its application. The Weissella confusa DY12 was isolated from sheep feces. The present invention identified the probiotic effect of Weissella confusa DY12 through animal experiments. The results showed that Weissella confusa DY12 could tolerate acid and bile salts and resist the gastrointestinal internal environment; its hydrophobicity was 39.47% and its auto-aggregation was 48.12%, demonstrating that the Weissella confusa DY12 of the present invention has good intestinal adhesion ability and has the potential of probiotics. The present invention verified that Weissella confusa DY12 can effectively regulate the immune function of the animal body, improve the antioxidant capacity of the body, as well as the content of lysozyme and the activity of alkaline phosphatase, and increase the daily weight gain, ultimately improving the growth performance of animals.

The invention discloses application of an active compound in an anti-cataract drug, and particularly relates to the field of drugs. The active compound is lenalidomide, mycophenolic acid, glycyrrhetinic acid or bromfenac sodium hydrate. Through a solution based on a CRYAB structure and animal experiment screening, it is found that the active compound can serve as an effective component of an anti-cataract drug, the progress of cataract can be remarkably relieved or inhibited, and a new strategy and potential drug selection are provided for treatment of cataract.

The invention relates to the technical field of animal experiment devices, and discloses a spatial cognition enhancement device and method for three-dimensional rotational stimulation of a vestibular-hippocampus pathway, and the device comprises a rotational stimulation unit which is used for generating multi-axial angular acceleration stimulation; the animal adaptation unit is connected with the rotary stimulation unit and is used for fixing an experimental animal and blocking visual input; the control unit is in signal connection with the rotary stimulation unit and is used for adjusting stimulation parameters and collecting dynamic data; and the signal acquisition unit comprises a piezoresistive sensor and an analog-to-digital converter and is used for acquiring kinetic parameters of angular velocity and angular acceleration in real time. According to the space cognition enhancement device and method for three-dimensional rotation stimulation of the vestibular-hippocampus pathway, real three-dimensional space motion is simulated through multi-axial rotation, and targeted stimulation of the vestibular-hippocampus pathway is achieved in combination with adjustable rotating speed and visual shielding maintenance. The device is integrated with an acoustic intervention module and supports multi-mode intervention.

The invention provides an application of cichorium glandulosum polysaccharide in preparation, basic characterization and type 2 diabetes mellitus relieving medicines, and belongs to the technical field of medicines. Animal experiment results show that after the cichorium glandulosum polysaccharide is applied to type 2 diabetic mice, serum biochemical indexes can be adjusted, the blood sugar level can be reduced, organ injuries can be protected or repaired, and PI3K / AKT / mTOR signal channel related protein expression in the main glycometabolism target organ liver can be adjusted. Therefore, the prepared cichorium glandulosum polysaccharide can be used as a medicine for relieving type 2 diabetes mellitus.

The invention discloses application of Trolox in preparation of a medicine for treating toxicity caused by valproic acid, and belongs to the technical field of biological medicine. The invention discovers for the first time that Trolox has a new medicine application of preventing and / or treating developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid; furthermore, animal experiments prove that Trolox can relieve zebra fish development malformation caused by valproic acid and also can relieve zebra fish redox level imbalance caused by valproic acid; this provides a new solution for the prevention and / or treatment of developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid.

The invention discloses an animal experiment acupuncture point massage parameter self-correction method combined with deep learning, and relates to the technical field of animal intervention experiments. The specific operation of the animal experiment acupoint massage parameter self-correction method combined with deep learning comprises the steps of simulating human hand tactile characteristic device design, deep learning model construction and training and massage parameter self-correction operation flow, and the human hand tactile characteristic device design comprises a tactile sensing module and a force feedback execution module. The step of deep learning model construction and training comprises data acquisition and labeling, model structure design and model training and optimization, and the step of massage parameter self-correction operation comprises an experiment preparation stage, an experiment proceeding stage and an experiment ending stage. Spatial feature data, including pressure distribution and strain images, collected by a multi-mode sensor can be effectively processed.

The invention relates to the technical field of animal experiment analysis, in particular to an animal behavior monitoring method, device and equipment and a medium. The method comprises the following steps: acquiring video stream data for a target area; determining target information of each animal in the video frame, wherein the target information comprises number information, center point information and attitude information; and performing behavior monitoring on the animals according to the number information, the center point information and the attitude information of the animals in each video frame to obtain animal behavior monitoring information. According to the method, continuous behavior monitoring, such as trajectory tracking, posture change, position change and other state or behavior monitoring, is carried out on the animals with different serial number information in combination with continuous video frames through the serial number information, the center point information and the posture information of the animals; the problems that in the prior art, influence of non-experimental factors is likely to happen, and the monitoring efficiency is low are solved.

The invention provides an animal multi-mode experiment control system for controlling the concentration of hydrogen and oxygen, an integrated sensor module, a gas concentration control system and a user interface. The sensor module monitors the temperature, the humidity, the hydrogen concentration and the oxygen concentration in the experiment module in real time and feeds back the data to the gas concentration control system, the actual flow value and the set flow value are compared through the central control PCB according to flow information provided by the float flowmeter, the opening degree of the valve is automatically adjusted, and the gas concentration is controlled. And the concentration of hydrogen and oxygen in the experiment module is maintained in a preset range. The high-precision sensor module can accurately capture key parameter changes. A PID algorithm is adopted for gas concentration control, a float flowmeter and an automatic regulating valve are combined, the concentration control precision within + / -1% is achieved, and the high-precision experiment requirement is met. According to the invention, a stable and accurate hydrogen and oxygen concentration management scheme is provided for animal experiments, and the controllability and data reliability of the experiments are improved.

The invention relates to the technical field of animal experiments, and provides a small animal experiment operation platform which comprises a platform body, the platform body comprises an operation area used for placing small animals for experiment operation, vertical rods are evenly arranged on the bottom side of the operation area in a penetrating mode, and each vertical rod can independently ascend and descend. The operation platform further comprises a plurality of pressing pieces used for being arranged on the vertical rods in a sleeving mode to press and fix the small animals. After a small animal to be experimented is placed in the operation area, a fixing part is determined according to the actual size of the small animal body and the experimental design, then the vertical rods near the fixing part are sequentially selected and moved upwards by a certain distance, and then the vertical rods moving upwards are sequentially sleeved with pressing pieces matched with the fixing part. The vertical rods which are uniformly distributed in a dot-matrix manner are used as fixing anchor points, and then the pressing sheet is sleeved on the fixing anchor points for pressing and fixing, so that the fixing requirements of different body sizes and custom fixing parts are met, and the flexibility and the accuracy of small animal experiment operation are greatly improved.

The invention provides application of bifidobacterium breve in preparation of a product for resisting gastric ulcer and gastric inflammation caused by helicobacter pylori, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve NKU BB1-2 is GDMCC NO: 65956, and the bifidobacterium breve NKU BB1-2 can antagonize helicobacter pylori colonization and relieve gastric ulcer aggravation caused by helicobacter pylori. The bifidobacterium breve NKU BB 1-2 has good helicobacter pylori antagonism ability in an in-vitro environment, and can significantly inhibit colonization growth of helicobacter pylori; animal experiments show that the bifidobacterium breve NKU BB 1-2 can relieve gastric ulcer aggravation caused by helicobacter pylori infection in vivo, and the gastric mucosa lesion is relieved, the stomach inflammation is relieved, and the stomach pH value is increased; in addition, the bifidobacterium breve NKU BB1-2 can also significantly improve the diversity of stomach microorganisms, regulate and improve the structure of the stomach microorganisms, and assist antagonism and removal of helicobacter pylori infection.

The invention provides an animal experiment time-limited feeding method and system. The animal experiment time-limited feeding method and system are applied to an intelligent door control system comprising a feeding box body, an electric control door control channel, a multi-mode sensor array and an intelligent control unit. According to the method, monitoring data streams of animal states and behaviors are obtained through a multi-modal sensor array; implementing execution control of the time-limited feeding mode based on the monitoring data stream and preset parameters; outputting a personalized time-limited feeding intervention strategy based on the execution data and the monitoring data stream; and management and analysis of experimental data are realized through the data management module. The invention further provides a corresponding time-limited feeding system, the system has dynamic adaptability, comprehensive monitoring dimension, high flexibility and deep data integration capacity, the problems that in the prior art, manual control accuracy is poor, intelligent adaptability and data deep mining are lacked are solved, and the system is suitable for large-scale popularization and application. And the accuracy, scientificity and animal welfare level of animal experiments are improved.

The invention belongs to the field of biological pharmacy, and relates to an alpha-amylase inhibitory peptide and application thereof. Specifically, the provided unreported peptide fragment LDKFLWT can effectively inhibit the activity of alpha-amylase, and animal experiments prove that the newly designed polypeptide has the effects of reducing blood sugar, reducing blood fat, improving insulin resistance and the like through polypeptide intervention; compared with a traditional hypoglycemic drug, the alpha-amylase inhibitory peptide disclosed by the invention has remarkable safety.

A use of liquiritin in the preparation of a drug for treating atopic dermatitis. Animal experiments show that liquiritin can remarkably lessen the skin lesion severity of an atopic dermatitis mouse model, reduce the ear thickness and the epidermal thickness, and reduce the infiltration degree of dermal lymphocytes, thereby demonstrating that liquiritin has a significant therapeutic effect on atopic dermatitis, has no toxic and side effects on the liver, the spleen and the kidney, and has good clinical application prospects and enormous social benefits, and expanding a new application field for a conventional leguminous plant liquorice extract, i.e., liquiritin.

This invention belongs to the field of biomedicine and specifically relates to the use of β-hydroxybutyrate in the preparation of a drug for the treatment of endometriosis. In animal experiments, BHB treatment significantly reduced endometriosis lesions between days 4 to 18 and 28 after modeling. Clinical studies have also found decreased serum BHB levels in endometriosis patients, suggesting that BHB supplementation may improve endometriosis symptoms. This suggests that BHB has a beneficial therapeutic effect on endometriosis and holds promise for clinical application.

The invention discloses application of Trolox in preparation of a medicine for treating autism, and belongs to the technical field of biological medicine. The invention finds that Trolox has the effect of preventing and / or treating autism for the first time, and further, animal experiments prove that Trolox prevents and / or treats autism by relieving repeated engraving behaviors of autism rats, rescuing social novelty disorders of the autism rats and reducing anxiety behaviors of the autism rats. The invention provides a theoretical basis and a new drug source for prevention and / or treatment of autism.

The application provides a method for analyzing the synergistic mechanism of hepatotoxicity and nephrotoxicity of non-steroidal anti-inflammatory drugs. The method comprises the following steps: preliminary toxicity prediction of NSAIDs and collection of toxicity target points, collection of liver and kidney disease target points, then cross and screening of the target points to obtain core target points and common core target points of NSAIDs induced liver and kidney diseases, and then constructing a protein interaction network of the common core target points; enrichment analysis of the common core target points to obtain the common action pathway of NSAIDs induced liver and kidney diseases; finally, further screening of the common core target points to obtain the key target points of NSAIDs induced liver and kidney diseases, and verification by using molecular docking technology. Compared with the traditional method, the advantages of the method are: first, the method does not depend on large-scale patient clinical data and a large number of animal or cell experiments, avoiding the ethical controversy in animal experiments and human experiments; second, the method can identify the potential cross-pathway and synergistic toxicity mechanism when a compound triggers multiple diseases, which is helpful for more comprehensive evaluation of the toxicity risk of NSAIDs.

The invention provides a traditional Chinese medicine composition for treating nephropathy as well as a preparation method and application thereof, and the anti-renal fibrosis effect is remarkably improved by optimizing a leech extraction process and a novel preparation technology. Animal experiments show that urine protein of model animals can be reduced by 67% and serum creatinine can be reduced by 42% after 12 weeks of continuous administration. Clinical tests prove that the traditional Chinese medicine composition has a definite curative effect on IgA nephropathy, membranous nephropathy and the like, and a new choice is provided for treating chronic kidney diseases.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

The invention relates to application of tripterygium glycosides tablets to improvement of central sensitization pain. The invention provides application of a tripterygium glycoside tablet in preparation of a product. The product is used for preventing or treating central sensitization pain. Based on animal experiment verification, the inventor of the invention finds that the tripterygium glycoside tablet can be used for treating the central sensitization type pain, and a new treatment means is provided for the central sensitization type pain.

The invention relates to the technical field of animal experiments, and discloses a mouse cerebral infarction model establishment method and system.The mouse cerebral infarction model establishment method comprises the following steps that in the mouse cerebral infarction model operation process, mouse operation data are obtained in real time, and anomaly detection feedback is carried out, specifically, the anomaly detection feedback comprises generation of a suture insertion anomaly signal; if the suture insertion abnormal signal is generated, performing similarity analysis of curve shapes and characteristics in combination with a historical database of the mouse cerebral infarction model surgery, and constructing a significant similar curve set; and analyzing the effectiveness of the mouse cerebral infarction model operation in the significant similar curve set, and determining the adjustability of the current mouse cerebral infarction operation abnormality. According to the method, the pressure and depth data are collected in real time, the anomaly detection coefficient is calculated, immediate early warning of the suture insertion anomaly is carried out, whether adjustment is needed or an operation is ended is analyzed after early warning, the subjectivity of traditional experience judgment is reduced, and the success rate of the operation is increased.

The invention relates to the field of animal experiments, and discloses an animal experiment system based on concealed transportation and self-purification and a management method.The animal experiment system comprises a feeding module and a control module, the feeding module comprises a plurality of independent cages, and temperature and humidity sensors, miniature air supply and exhaust modules and cameras are arranged in the independent cages; the transportation module comprises a concealed rail, and the concealed rail is used for connecting the feeding module and the operation module; the operation module comprises a lifting type operation table, the lifting type operation table is provided with a mechanical arm and an isolation barrier, and the operation module further comprises a health monitoring station. Low-noise and stable hidden transportation is achieved through the magnetic suspension driving technology of a hidden track and a sound insulation shielding layer covering the outside, the bottom of each independent cage is provided with a microbial degradation layer composed of an immobilized bacillus subtilis film and a porous ceramic substrate, pollutants can be continuously and efficiently decomposed, peculiar smells and bacterium breeding are reduced, and the environment is protected. Therefore, experimental data can truly and accurately reflect the influence of experimental variables.

The present invention relates to the field of food and medicine, and in particular to a red apple and cherry plum composition with anti-atherosclerotic efficacy, wherein the weight ratio of the red apple and cherry plum is 1:10-10:1. Mouse animal experiments show that the red apple and cherry plum composition has a synergistic effect in anti-atherosclerotic efficacy, and the efficacy of the composition in preventing or treating atherosclerosis is better than that of using red apple or cherry plum alone. The product forms of the red apple and cherry plum composition include juice, jam, fruit powder, oral liquid products, capsules, tablets, etc. The red apple and cherry plum composition also has a certain synergistic effect when used in combination with statins and other drugs.

The invention discloses application of LW6 in resisting vomitoxin, and belongs to the field of biological agents. Animal experiments are carried out on male C57BL mice, LW6 and DON are injected in a gavage mode to explore the DON tolerance influence of the mice, it is found that the LW6 can reduce harm to animal bodies caused by DON toxicity, and the DON tolerance of animals is improved. Therefore, the LW6 can be applied to a feed additive or developed into an anti-DON drug so as to reduce the toxic influence of DON on animals. The invention provides a potential solution way for preventing adverse reaction caused by DON, and provides a theoretical basis for preventing and controlling harm of DON to pig industry.

The present invention relates to the field of medical technology, and specifically to the use of salvianolic acid B in the preparation of drugs for treating IPEX. The present invention proves through in vivo animal experiments that salvianolic acid B has a good anti-inflammatory effect and can effectively alleviate the autoimmune reaction of Treg-deficient SF mice. In the present invention, the cations in the salvianolic acid B salts can be iron ions, calcium ions, sodium ions, potassium ions or magnesium ions, preferably magnesium ions; the dosage form of the drug for treating IPEX can be any dosage form described in pharmacy, and can be an oral liquid form, an injection form, a tablet form or a capsule form. By utilizing the key role of salvianolic acid B in IPEX disease, it can be prepared into a drug, providing a theoretical basis and a new treatment idea for the treatment of primary immunodeficiency diseases with traditional Chinese medicine; salvianolic acid B is easy to obtain, can be quickly put into production and obtain a high-efficiency preparation, and has important application value.

The utility model belongs to the field of blood sampling and positioning for animal experiments, particularly relates to blood sampling and positioning equipment, and aims to solve the problems that although the neck of an animal can be clamped through a clamping block in use in the prior art, the clamping pressure cannot be detected in the clamping process, and discomfort or injury is easily caused when the animal is fixed. The device comprises a base, a frame body is welded to the top of the base, the frame body is in a U shape, moving grooves are formed in the inner walls of the two sides of the frame body, the inner walls of the two moving grooves are slidably connected with the same frame body, and a second concave plate is fixedly arranged on the inner wall of one side of the frame body; the inner wall of the top and the inner wall of the bottom of the frame body are slidably connected with the same first concave plate, the neck of an animal can be clamped through the clamping mechanism, the height of the clamping block can be adjusted through the adjusting mechanism, and the clamping force of the clamping block is detected through the detection mechanism, so that unnecessary damage to the animal is avoided.