383 results about "Animals experiments" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention relates to the technical field of microorganisms, and particularly discloses bifidobacterium adolescentis AGE17 capable of promoting growth and development of muscles and bones and application of the bifidobacterium adolescentis AGE17. The bifidobacterium adolescentis AGE17 is separated from excrement of healthy children and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33452, and the preservation date is January 17, 2025. The bifidobacterium adolescentis AGE17 has the advantages that the bifidobacterium adolescentis AGE17 can be used for preparing the biological organic fertilizer; animal experiments prove that the bifidobacterium adolescentis strain AGE17 provided by the invention has the function of promoting muscle and skeletal development, has more obvious and prominent effects compared with a model strain bifidobacterium adolescentis ATCC15703, and has important application value in increasing the muscle strength and height of children and adolescents.

The invention relates to the technical field of animal experiment equipment, and discloses an isolated culture device for mouse sweet water preference test in an IVC cage, which comprises a shell cover, the partition plate is fixedly installed on the inner side wall of the shell cover, and an inner cavity of the shell cover is divided into a living area and a functional area through the partition plate; the sliding cover is arranged in the living area of the shell cover and used for containing solid feed, and multiple sets of feeding trough openings which are arranged at equal intervals are formed in the bottom of the sliding cover; according to the device, an innovative cage-in-cage design is adopted, a single cage is converted into a plurality of independent experiment units on the premise that an existing IVC system is not changed, and cage position resources are greatly saved. The functions of isolation, double-bottle water drinking and independent feeding are integrated, and convenient operation is achieved through the pull-out type fixing plate and the sliding cover plate. The centrifugal tube is adopted to replace a special water bottle, so that the cost is reduced, the SPF environment is kept in the whole process, data accuracy and reliability are ensured, and the traditional SPT test problem is effectively solved.

The invention discloses application of an active compound in an anti-cataract drug, and particularly relates to the field of drugs. The active compound is lenalidomide, mycophenolic acid, glycyrrhetinic acid or bromfenac sodium hydrate. Through a solution based on a CRYAB structure and animal experiment screening, it is found that the active compound can serve as an effective component of an anti-cataract drug, the progress of cataract can be remarkably relieved or inhibited, and a new strategy and potential drug selection are provided for treatment of cataract.

The invention relates to the technical field of animal experiment devices, and discloses a spatial cognition enhancement device and method for three-dimensional rotational stimulation of a vestibular-hippocampus pathway, and the device comprises a rotational stimulation unit which is used for generating multi-axial angular acceleration stimulation; the animal adaptation unit is connected with the rotary stimulation unit and is used for fixing an experimental animal and blocking visual input; the control unit is in signal connection with the rotary stimulation unit and is used for adjusting stimulation parameters and collecting dynamic data; and the signal acquisition unit comprises a piezoresistive sensor and an analog-to-digital converter and is used for acquiring kinetic parameters of angular velocity and angular acceleration in real time. According to the space cognition enhancement device and method for three-dimensional rotation stimulation of the vestibular-hippocampus pathway, real three-dimensional space motion is simulated through multi-axial rotation, and targeted stimulation of the vestibular-hippocampus pathway is achieved in combination with adjustable rotating speed and visual shielding maintenance. The device is integrated with an acoustic intervention module and supports multi-mode intervention.

The invention discloses application of Trolox in preparation of a medicine for treating toxicity caused by valproic acid, and belongs to the technical field of biological medicine. The invention discovers for the first time that Trolox has a new medicine application of preventing and / or treating developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid; furthermore, animal experiments prove that Trolox can relieve zebra fish development malformation caused by valproic acid and also can relieve zebra fish redox level imbalance caused by valproic acid; this provides a new solution for the prevention and / or treatment of developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid.

The invention provides application of bifidobacterium breve in preparation of a product for resisting gastric ulcer and gastric inflammation caused by helicobacter pylori, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve NKU BB1-2 is GDMCC NO: 65956, and the bifidobacterium breve NKU BB1-2 can antagonize helicobacter pylori colonization and relieve gastric ulcer aggravation caused by helicobacter pylori. The bifidobacterium breve NKU BB 1-2 has good helicobacter pylori antagonism ability in an in-vitro environment, and can significantly inhibit colonization growth of helicobacter pylori; animal experiments show that the bifidobacterium breve NKU BB 1-2 can relieve gastric ulcer aggravation caused by helicobacter pylori infection in vivo, and the gastric mucosa lesion is relieved, the stomach inflammation is relieved, and the stomach pH value is increased; in addition, the bifidobacterium breve NKU BB1-2 can also significantly improve the diversity of stomach microorganisms, regulate and improve the structure of the stomach microorganisms, and assist antagonism and removal of helicobacter pylori infection.

The invention provides an animal experiment time-limited feeding method and system. The animal experiment time-limited feeding method and system are applied to an intelligent door control system comprising a feeding box body, an electric control door control channel, a multi-mode sensor array and an intelligent control unit. According to the method, monitoring data streams of animal states and behaviors are obtained through a multi-modal sensor array; implementing execution control of the time-limited feeding mode based on the monitoring data stream and preset parameters; outputting a personalized time-limited feeding intervention strategy based on the execution data and the monitoring data stream; and management and analysis of experimental data are realized through the data management module. The invention further provides a corresponding time-limited feeding system, the system has dynamic adaptability, comprehensive monitoring dimension, high flexibility and deep data integration capacity, the problems that in the prior art, manual control accuracy is poor, intelligent adaptability and data deep mining are lacked are solved, and the system is suitable for large-scale popularization and application. And the accuracy, scientificity and animal welfare level of animal experiments are improved.

This invention belongs to the field of biomedicine and specifically relates to the use of β-hydroxybutyrate in the preparation of a drug for the treatment of endometriosis. In animal experiments, BHB treatment significantly reduced endometriosis lesions between days 4 to 18 and 28 after modeling. Clinical studies have also found decreased serum BHB levels in endometriosis patients, suggesting that BHB supplementation may improve endometriosis symptoms. This suggests that BHB has a beneficial therapeutic effect on endometriosis and holds promise for clinical application.

The invention discloses application of Trolox in preparation of a medicine for treating autism, and belongs to the technical field of biological medicine. The invention finds that Trolox has the effect of preventing and / or treating autism for the first time, and further, animal experiments prove that Trolox prevents and / or treats autism by relieving repeated engraving behaviors of autism rats, rescuing social novelty disorders of the autism rats and reducing anxiety behaviors of the autism rats. The invention provides a theoretical basis and a new drug source for prevention and / or treatment of autism.

The application provides a method for analyzing the synergistic mechanism of hepatotoxicity and nephrotoxicity of non-steroidal anti-inflammatory drugs. The method comprises the following steps: preliminary toxicity prediction of NSAIDs and collection of toxicity target points, collection of liver and kidney disease target points, then cross and screening of the target points to obtain core target points and common core target points of NSAIDs induced liver and kidney diseases, and then constructing a protein interaction network of the common core target points; enrichment analysis of the common core target points to obtain the common action pathway of NSAIDs induced liver and kidney diseases; finally, further screening of the common core target points to obtain the key target points of NSAIDs induced liver and kidney diseases, and verification by using molecular docking technology. Compared with the traditional method, the advantages of the method are: first, the method does not depend on large-scale patient clinical data and a large number of animal or cell experiments, avoiding the ethical controversy in animal experiments and human experiments; second, the method can identify the potential cross-pathway and synergistic toxicity mechanism when a compound triggers multiple diseases, which is helpful for more comprehensive evaluation of the toxicity risk of NSAIDs.

The invention provides a traditional Chinese medicine composition for treating nephropathy as well as a preparation method and application thereof, and the anti-renal fibrosis effect is remarkably improved by optimizing a leech extraction process and a novel preparation technology. Animal experiments show that urine protein of model animals can be reduced by 67% and serum creatinine can be reduced by 42% after 12 weeks of continuous administration. Clinical tests prove that the traditional Chinese medicine composition has a definite curative effect on IgA nephropathy, membranous nephropathy and the like, and a new choice is provided for treating chronic kidney diseases.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

The invention relates to the field of animal experiments, and discloses an animal experiment system based on concealed transportation and self-purification and a management method.The animal experiment system comprises a feeding module and a control module, the feeding module comprises a plurality of independent cages, and temperature and humidity sensors, miniature air supply and exhaust modules and cameras are arranged in the independent cages; the transportation module comprises a concealed rail, and the concealed rail is used for connecting the feeding module and the operation module; the operation module comprises a lifting type operation table, the lifting type operation table is provided with a mechanical arm and an isolation barrier, and the operation module further comprises a health monitoring station. Low-noise and stable hidden transportation is achieved through the magnetic suspension driving technology of a hidden track and a sound insulation shielding layer covering the outside, the bottom of each independent cage is provided with a microbial degradation layer composed of an immobilized bacillus subtilis film and a porous ceramic substrate, pollutants can be continuously and efficiently decomposed, peculiar smells and bacterium breeding are reduced, and the environment is protected. Therefore, experimental data can truly and accurately reflect the influence of experimental variables.

The utility model belongs to the field of blood sampling and positioning for animal experiments, particularly relates to blood sampling and positioning equipment, and aims to solve the problems that although the neck of an animal can be clamped through a clamping block in use in the prior art, the clamping pressure cannot be detected in the clamping process, and discomfort or injury is easily caused when the animal is fixed. The device comprises a base, a frame body is welded to the top of the base, the frame body is in a U shape, moving grooves are formed in the inner walls of the two sides of the frame body, the inner walls of the two moving grooves are slidably connected with the same frame body, and a second concave plate is fixedly arranged on the inner wall of one side of the frame body; the inner wall of the top and the inner wall of the bottom of the frame body are slidably connected with the same first concave plate, the neck of an animal can be clamped through the clamping mechanism, the height of the clamping block can be adjusted through the adjusting mechanism, and the clamping force of the clamping block is detected through the detection mechanism, so that unnecessary damage to the animal is avoided.

1. The name of the design product: animal experiment operating table. 2. The use of the design product: worktable for animal experiment operation. 3. The design points of the design product: the combination of shape and color. 4. The picture or photo that best shows the design points: perspective view 1. 5. The design claimed contains color.

The application discloses application of tomato saponin in preparation of a uric acid reducing composition and belongs to the technical field of biological medicine. The uric acid reducing composition comprises 80-240 parts of tomato saponin, 50-120 parts of momordica charantia saponin, 25-100 parts of fruit and vegetable extract, 10-20 parts of schizonepeta turczanovii extract, 10-20 parts of terminalia catappa leaf extract and 40-100 parts of soluble dietary mixture. Animal experiment verification shows that the composition can effectively reduce blood uric acid level and improve kidney function through the synergistic effect of multi-target points such as inhibition of uric acid synthesis, promotion of uric acid excretion, anti-inflammatory kidney protection and metabolic regulation, and a safe and efficient solution is provided for hyperuricemia.

The invention relates to a capsule type oxygen cabin for animal experiments, which adopts a modular structure, comprehensively considers the influence of multiple factors such as temperature, humidity, pressure and oxygen concentration, and also considers the influence of breathing oxygen consumption of experimental animals and the influence of a first / second accommodating part, and on the basis, the first / second accommodating part is centrally controlled through a touch screen; according to the method, the combination of multiple modes can be realized, so that the climate conditions of different regions can be accurately, efficiently and stably simulated, and accurate and scientific guidance can be provided for the research on'high reflection ', the work and life of personnel in high and cold climate regions and the pathology research of hospitals. Besides, the electrical parts are classified and placed in the first accommodating part, the second accommodating part, the touch screen and the bearing part, so that the cabin body can be transparent and visual, and the electrical parts in the first accommodating part and the second accommodating part are convenient to overhaul through detachable connection between the first partition plate and the second partition plate and the first accommodating part and between the first partition plate and the second partition plate and the second accommodating part.

The utility model discloses an animal experiment rotating rod fatigue instrument convenient to disassemble, and belongs to the technical field of fatigue instruments, the animal experiment rotating rod fatigue instrument convenient to disassemble comprises a fatigue instrument body, a plurality of partition plates are arranged in the fatigue instrument body at equal intervals, and a same rotating rod is arranged in the middles of the partition plates in a penetrating mode; a collecting frame is slidably connected to the bottom of the fatigue instrument body, a supporting frame is fixedly connected to the rear side of the fatigue instrument body, a clamping mechanism is arranged on the inner side of the supporting frame, a pair of clamping blocks is fixedly connected to the rear end of a partition plate, and a driving motor and a threaded cylinder are arranged on the two sides of a rotating rod correspondingly; according to the scheme, the rotating rod assembly with a simple structure is arranged, the dismounting work can be quickly carried out, the subsequent cleaning and disinfection work of the fatigue instrument is facilitated, and the clamping assembly is arranged, so that the partition piece can be quickly clamped, and the adjustment of the experiment distance is facilitated, so that different experiment conditions are adapted.

The invention discloses an application of a prebiotic component in enhancing athletic ability and improving athletic performance. The prebiotic component is xylooligosaccharide. Animal experiments show that mice given with xylooligosaccharide can significantly improve the abundance of probiotics and enhance the muscle strength, constant-speed endurance and other movement functions of the mice. The xylooligosaccharide serving as an efficient prebiotic can be specifically utilized by specific probiotics in intestinal tracts, the abundance of the xylooligosaccharide is remarkably improved, growth of pathogenic bacteria is inhibited, then the micro-ecological environment of the intestinal tracts is optimized, and the exercise function improvement phenomena such as increase of muscle strength and delay of fatigue in endurance exercise are shown in behavioral tests. The cross-organ integration regulation and control from the intestinal microecology to the whole body physiological state and then to the athletic performance is reflected, so that the xylooligosaccharide has important significance in enhancing the athletic ability and improving the athletic performance.

The application provides a cage position and cage box intelligent identification method, a storage medium and an electronic device. The method comprises the following steps: inputting a picture of a cage frame into a pre-trained YOLOv8 detection model, obtaining a plurality of target images in the picture of the cage frame and pixel coordinates of each target image, and saving each target image according to a category of each target image; the category of each target image comprises an empty cage position, an empty cage box and cage card information; when the total number of cage positions is equal to the actual total number of cage frames, obtaining a target image corresponding to the cage card information and a pixel coordinate of the target image corresponding to the cage card information; and outputting each cage position information of the cage frame based on the obtained target image corresponding to the cage card information and the pixel coordinate of the target image corresponding to the cage card information. The cage position and cage box intelligent identification method provided by the application has the advantages of high detection precision, fast detection speed and high detection accuracy, can batch detect and identify cage positions and cage boxes, and effectively improves the management level of an animal experiment center.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The application belongs to the technical field of toxicity test, and discloses a high-repetitive and high-precision exposure and poisoning animal experiment system and method. The exposure and poisoning animal experiment system comprises an animal exposure and poisoning experiment unit. The aerosol gas inlet sleeve, the poisoning exposure cabin, the isolation mouse cage, the horn-shaped upper guide cylinder and the cylindrical lower guide cylinder jointly form two mutually isolated poisoning exposure channels. Each channel independently inputs poisoning exposure mixed gas into a poisoning exposure chamber. The animal poisoning exposure experiment in the oral-nasal exposure mode or the whole-body exposure mode can be completed by using one set of experiment system. Through the synchronous improvement of the experiment system structure and the experiment method, the oral-nasal or whole-body exposure mode can be flexibly switched, and the micro-sample high-concentration exposure experiment of multiple poisoning exposure chambers can be simultaneously performed. The accuracy and repeatability of the animal poisoning exposure experiment are significantly improved. The application breaks through the limitations of the existing experiment system and method, and has a wide application prospect.

The application discloses a kind of multi-camera-based animal vital sign monitoring method, medium, equipment, it is related to animal vital sign monitoring technical field, method includes: using DLC posture estimation network to train animal multi-view video, obtain key point information;According to the key point information of main visual angle, the centroid motion of animal is analyzed and compared with motion threshold, determine animal state, in the continuous image sequence of relative static state time period, according to key point information, obtain region of interest;Corner point is detected in region of interest, and corresponding motion signal is acquired by tracking corner point;Correlation analysis is carried out to motion signal, and sorting is carried out based on information gain, and vital sign signal is obtained by fusing motion signal higher than set threshold;Power spectrum is used to analyze vital sign signal, obtain the energy distribution of vital sign signal, and the value of respiratory rate and heart rate is calculated.The application can effectively reduce artificial intervention, and has the role of promoting to animal experiment method innovation and data analysis.

The utility model provides a firm animal experiment binding device, and belongs to the technical field of animal experiments. Comprising an arc-shaped base, a C-shaped frame is arranged at the top of the arc-shaped base, the top of the C-shaped frame is in threaded connection with a first screw rod, and the bottom end of the first screw rod is rotationally connected with an arc-shaped clamping base. The four limbs of an experimental animal can be clamped and fixed between the clamping plate and the side wall of the binding seat through the four limb binding assembly, the neck of the experimental animal can be clamped and fixed between the arc-shaped base and the arc-shaped clamping seat through the neck clamping assembly, and the body of the experimental animal can be bound through the hook-and-loop fastener and the hook-and-loop fastener. An experimental animal can be firmly bound to the top of the bottom plate, the situation that the experimental process is affected by twisting and shaking of four limbs, the neck and the body of the animal in the experimental process is effectively avoided, and the using effect is greatly improved.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

The utility model relates to a mouse head fixing operation device, and belongs to the technical field of animal experiment equipment, the mouse head fixing operation device comprises a heatable bottom plate and a clamp assembly used for limiting upper incisor teeth and mouth of a mouse, two stand columns with holes are fixed on the heatable bottom plate, a cross rod is arranged between the two stand columns with holes in a sliding mode, and the cross rod slides in the vertical direction; long movable arms are arranged on the two stand columns with the holes, one ends of the long movable arms are arranged on the stand columns with the holes in a sliding mode in the vertical direction, the other ends of the long movable arms are arranged in an overhanging mode in the direction away from the clamp assembly, ear rods are arranged at the overhanging ends of the two long movable arms in a sliding mode, and the two ear rods slide in the direction close to or away from each other; the device has the advantages that the head of a mouse is further stabilized, secondary damage to the mouse caused by an operation device is avoided, the body temperature of the animal in the operation process is kept constant, and the success rate of the operation and the accuracy of experimental data are guaranteed.

The invention belongs to the technical field of medicines, and particularly relates to a phenanthroindolizidine alkaloid compound and application thereof. The invention discloses two kinds of phenanthroindolizidine alkaloid compounds, namely (-)-(R)-13a alpha-secoxanthin and (-)-(R)-13a alpha-6-O-desmylanthin, which are derived from a traditional Chinese medicine cynanchum atratum, and discloses the obvious anti-neuritis activity of the phenanthroindolizidine alkaloid compounds for inhibiting the generation of nitric oxide. Animal experiments prove that by intraperitoneal injection of the (-)-(R)-13a alpha-secoxanthin and the (-)-13a alpha-6-O-desmylanthin, the autonomous movement ability of a depression mouse is remarkably improved, the exploration behavior of the mouse is recovered, the anxiety symptom of the mouse is relieved, and the despair behavior of the mouse is relieved. The invention provides a new lead compound for research and development of new antidepressant drugs.

The invention discloses a brain surgery auxiliary spreader based on animal experiments, and relates to the technical field of spreader, the brain surgery auxiliary spreader comprises two spreading frames and a hinge frame, the spreading frames are hollow special-shaped plates, the number of the spreading frames is two, and the two spreading frames are movably hinged to the hinge frame; the hinging frame is connected with the two opening frames through a driving part, and the driving part is used for driving the two opening frames to do opening and closing movement; an auxiliary plate with a hollow interior is fixed at the bottom of each opening frame far away from the hinging frame, and air holes are formed in the auxiliary plates close to the inner layer of the skin; a hollow bin is fixed to the position, away from the auxiliary plate, of each opening frame, each hollow bin is a hollow plate body and communicates with the interior of the corresponding opening frame, and the interiors of the opening frames communicate with the interiors of the auxiliary plates. A push-pull part is positioned in the hollow bin and used for increasing negative pressure in the distraction frame, the shape of the distraction skin can be better kept unchanged in the operation process, the skin is not prone to slipping, and safety in the operation process is ensured.