203 results about "Animals experiments" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention discloses application of an active compound in an anti-cataract drug, and particularly relates to the field of drugs. The active compound is lenalidomide, mycophenolic acid, glycyrrhetinic acid or bromfenac sodium hydrate. Through a solution based on a CRYAB structure and animal experiment screening, it is found that the active compound can serve as an effective component of an anti-cataract drug, the progress of cataract can be remarkably relieved or inhibited, and a new strategy and potential drug selection are provided for treatment of cataract.

The invention discloses application of Trolox in preparation of a medicine for treating toxicity caused by valproic acid, and belongs to the technical field of biological medicine. The invention discovers for the first time that Trolox has a new medicine application of preventing and / or treating developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid; furthermore, animal experiments prove that Trolox can relieve zebra fish development malformation caused by valproic acid and also can relieve zebra fish redox level imbalance caused by valproic acid; this provides a new solution for the prevention and / or treatment of developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid.

The invention discloses application of Trolox in preparation of a medicine for treating autism, and belongs to the technical field of biological medicine. The invention finds that Trolox has the effect of preventing and / or treating autism for the first time, and further, animal experiments prove that Trolox prevents and / or treats autism by relieving repeated engraving behaviors of autism rats, rescuing social novelty disorders of the autism rats and reducing anxiety behaviors of the autism rats. The invention provides a theoretical basis and a new drug source for prevention and / or treatment of autism.

The application provides a method for analyzing the synergistic mechanism of hepatotoxicity and nephrotoxicity of non-steroidal anti-inflammatory drugs. The method comprises the following steps: preliminary toxicity prediction of NSAIDs and collection of toxicity target points, collection of liver and kidney disease target points, then cross and screening of the target points to obtain core target points and common core target points of NSAIDs induced liver and kidney diseases, and then constructing a protein interaction network of the common core target points; enrichment analysis of the common core target points to obtain the common action pathway of NSAIDs induced liver and kidney diseases; finally, further screening of the common core target points to obtain the key target points of NSAIDs induced liver and kidney diseases, and verification by using molecular docking technology. Compared with the traditional method, the advantages of the method are: first, the method does not depend on large-scale patient clinical data and a large number of animal or cell experiments, avoiding the ethical controversy in animal experiments and human experiments; second, the method can identify the potential cross-pathway and synergistic toxicity mechanism when a compound triggers multiple diseases, which is helpful for more comprehensive evaluation of the toxicity risk of NSAIDs.

The invention provides a traditional Chinese medicine composition for treating nephropathy as well as a preparation method and application thereof, and the anti-renal fibrosis effect is remarkably improved by optimizing a leech extraction process and a novel preparation technology. Animal experiments show that urine protein of model animals can be reduced by 67% and serum creatinine can be reduced by 42% after 12 weeks of continuous administration. Clinical tests prove that the traditional Chinese medicine composition has a definite curative effect on IgA nephropathy, membranous nephropathy and the like, and a new choice is provided for treating chronic kidney diseases.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

The application discloses application of tomato saponin in preparation of a uric acid reducing composition and belongs to the technical field of biological medicine. The uric acid reducing composition comprises 80-240 parts of tomato saponin, 50-120 parts of momordica charantia saponin, 25-100 parts of fruit and vegetable extract, 10-20 parts of schizonepeta turczanovii extract, 10-20 parts of terminalia catappa leaf extract and 40-100 parts of soluble dietary mixture. Animal experiment verification shows that the composition can effectively reduce blood uric acid level and improve kidney function through the synergistic effect of multi-target points such as inhibition of uric acid synthesis, promotion of uric acid excretion, anti-inflammatory kidney protection and metabolic regulation, and a safe and efficient solution is provided for hyperuricemia.

The invention relates to a capsule type oxygen cabin for animal experiments, which adopts a modular structure, comprehensively considers the influence of multiple factors such as temperature, humidity, pressure and oxygen concentration, and also considers the influence of breathing oxygen consumption of experimental animals and the influence of a first / second accommodating part, and on the basis, the first / second accommodating part is centrally controlled through a touch screen; according to the method, the combination of multiple modes can be realized, so that the climate conditions of different regions can be accurately, efficiently and stably simulated, and accurate and scientific guidance can be provided for the research on'high reflection ', the work and life of personnel in high and cold climate regions and the pathology research of hospitals. Besides, the electrical parts are classified and placed in the first accommodating part, the second accommodating part, the touch screen and the bearing part, so that the cabin body can be transparent and visual, and the electrical parts in the first accommodating part and the second accommodating part are convenient to overhaul through detachable connection between the first partition plate and the second partition plate and the first accommodating part and between the first partition plate and the second partition plate and the second accommodating part.

The invention discloses an application of a prebiotic component in enhancing athletic ability and improving athletic performance. The prebiotic component is xylooligosaccharide. Animal experiments show that mice given with xylooligosaccharide can significantly improve the abundance of probiotics and enhance the muscle strength, constant-speed endurance and other movement functions of the mice. The xylooligosaccharide serving as an efficient prebiotic can be specifically utilized by specific probiotics in intestinal tracts, the abundance of the xylooligosaccharide is remarkably improved, growth of pathogenic bacteria is inhibited, then the micro-ecological environment of the intestinal tracts is optimized, and the exercise function improvement phenomena such as increase of muscle strength and delay of fatigue in endurance exercise are shown in behavioral tests. The cross-organ integration regulation and control from the intestinal microecology to the whole body physiological state and then to the athletic performance is reflected, so that the xylooligosaccharide has important significance in enhancing the athletic ability and improving the athletic performance.

The application provides a cage position and cage box intelligent identification method, a storage medium and an electronic device. The method comprises the following steps: inputting a picture of a cage frame into a pre-trained YOLOv8 detection model, obtaining a plurality of target images in the picture of the cage frame and pixel coordinates of each target image, and saving each target image according to a category of each target image; the category of each target image comprises an empty cage position, an empty cage box and cage card information; when the total number of cage positions is equal to the actual total number of cage frames, obtaining a target image corresponding to the cage card information and a pixel coordinate of the target image corresponding to the cage card information; and outputting each cage position information of the cage frame based on the obtained target image corresponding to the cage card information and the pixel coordinate of the target image corresponding to the cage card information. The cage position and cage box intelligent identification method provided by the application has the advantages of high detection precision, fast detection speed and high detection accuracy, can batch detect and identify cage positions and cage boxes, and effectively improves the management level of an animal experiment center.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The application discloses a kind of multi-camera-based animal vital sign monitoring method, medium, equipment, it is related to animal vital sign monitoring technical field, method includes: using DLC posture estimation network to train animal multi-view video, obtain key point information;According to the key point information of main visual angle, the centroid motion of animal is analyzed and compared with motion threshold, determine animal state, in the continuous image sequence of relative static state time period, according to key point information, obtain region of interest;Corner point is detected in region of interest, and corresponding motion signal is acquired by tracking corner point;Correlation analysis is carried out to motion signal, and sorting is carried out based on information gain, and vital sign signal is obtained by fusing motion signal higher than set threshold;Power spectrum is used to analyze vital sign signal, obtain the energy distribution of vital sign signal, and the value of respiratory rate and heart rate is calculated.The application can effectively reduce artificial intervention, and has the role of promoting to animal experiment method innovation and data analysis.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

The utility model relates to a mouse head fixing operation device, and belongs to the technical field of animal experiment equipment, the mouse head fixing operation device comprises a heatable bottom plate and a clamp assembly used for limiting upper incisor teeth and mouth of a mouse, two stand columns with holes are fixed on the heatable bottom plate, a cross rod is arranged between the two stand columns with holes in a sliding mode, and the cross rod slides in the vertical direction; long movable arms are arranged on the two stand columns with the holes, one ends of the long movable arms are arranged on the stand columns with the holes in a sliding mode in the vertical direction, the other ends of the long movable arms are arranged in an overhanging mode in the direction away from the clamp assembly, ear rods are arranged at the overhanging ends of the two long movable arms in a sliding mode, and the two ear rods slide in the direction close to or away from each other; the device has the advantages that the head of a mouse is further stabilized, secondary damage to the mouse caused by an operation device is avoided, the body temperature of the animal in the operation process is kept constant, and the success rate of the operation and the accuracy of experimental data are guaranteed.

The invention belongs to the technical field of medicines, and particularly relates to a phenanthroindolizidine alkaloid compound and application thereof. The invention discloses two kinds of phenanthroindolizidine alkaloid compounds, namely (-)-(R)-13a alpha-secoxanthin and (-)-(R)-13a alpha-6-O-desmylanthin, which are derived from a traditional Chinese medicine cynanchum atratum, and discloses the obvious anti-neuritis activity of the phenanthroindolizidine alkaloid compounds for inhibiting the generation of nitric oxide. Animal experiments prove that by intraperitoneal injection of the (-)-(R)-13a alpha-secoxanthin and the (-)-13a alpha-6-O-desmylanthin, the autonomous movement ability of a depression mouse is remarkably improved, the exploration behavior of the mouse is recovered, the anxiety symptom of the mouse is relieved, and the despair behavior of the mouse is relieved. The invention provides a new lead compound for research and development of new antidepressant drugs.

The utility model relates to the technical field of electric coagulation haemostatic forceps, and discloses a miniature electric coagulation haemostatic forceps for animal test surgery, which comprises two connecting rods, the left sides of the two connecting rods are fixedly connected with extension rods, the exteriors of the two extension rods are connected with sliding sleeves in a sliding manner, elastic assemblies are arranged in the extension rods, and the elastic assemblies are connected with the sliding sleeves in a sliding manner. A sliding groove is formed in the left side of the interior of the extension rod, a hemisphere is fixedly connected to the bottom of the elastic assembly, a plurality of clamping grooves are formed in the bottom side of the interior of the sliding sleeve, an outer clamp body is fixedly connected to the left side of one connecting rod, and an inner clamp body is fixedly connected to the left side of the other connecting rod. According to the haemostatic forceps, after the hemisphere moves to the designated clamping groove position, the spring releases elastic potential energy, the hemisphere is pushed to reset and be clamped in the clamping groove in the bottom of the sliding sleeve, and therefore the length of the haemostatic forceps is locked. The length of the hemostatic forceps can be flexibly adjusted according to the requirements of different operation scenes, and a doctor can operate and use the hemostatic forceps conveniently.

The present application belongs to the technical field of nutritional functional food and health food, and particularly relates to a preparation method of marine fish skin collagen oligopeptide beneficial to promoting skin health, and a product and application thereof. The method comprises the following steps: after deep sea fish skin is pretreated by salt dissolution with a NaCl solution, trisodium citrate is added as an enzymolysis aid, and neutral protease (from Trichoderma reesei) and papain are sequentially used for two-step enzymolysis, and after enzyme inactivation, impurities are removed, sterilization is performed, and concentration, drying are performed to obtain the product. In vitro cell experiment verification shows that the product can significantly promote human skin fibroblast proliferation; in vivo animal experiment verification shows that the product can significantly increase skin moisture content, reduce transdermal water loss, increase hydroxyproline content, improve SOD activity and reduce MDA content. The product is suitable for the field of nutritional functional food and health food, and has a good application prospect in promoting skin health.

The invention relates to a preparation method of an animal anti-diarrhea feed additive containing delinted moxa powder and essential oil. The preparation method of the animal anti-diarrhea feed additive containing the delinted moxa powder and the essential oil comprises the following steps: mixing essential oil components thymol and cinnamyl aldehyde, and stirring at 35-65 DEG C until the essential oil components are dissolved to obtain an essential oil solution; the preparation method comprises the following steps: mixing antioxidants BHA and PG, citric acid and ethanol, and stirring until dissolving to obtain an ethanol solution; mixing the ethanol solution with stone powder, and drying to remove ethanol to obtain a solid mixture; mixing the solid mixture with the essential oil solution, adding delinted moxa powder, and stirring uniformly; according to the preparation method of the animal anti-diarrhea feed additive containing the delinted moxa powder and the essential oil, stable combination of the essential oil and the delinted moxa powder is achieved, the diarrhea morbidity and mortality are remarkably reduced in animal experiments, and the effect is better than that of independent use of the essential oil.

The invention discloses a multi-mode simulation device and method for intraocular pressure. The simulation device comprises a track vertically arranged on the base table, a clamp is connected to the track in a sliding mode, a first liquid storage pipe with an opening in the top is installed on the clamp, the first liquid storage pipe is connected with a second liquid storage pipe through a flexible connecting pipe, and the first liquid storage pipe, the second liquid storage pipe and the flexible connecting pipe are filled with testing liquid; a temperature control module comprising a temperature sensor and a heater is arranged on the outer wall of the second liquid storage pipe; a simulated intraocular pressure measuring end is arranged at the top of the second liquid storage tube; and a distance measurement display module is arranged right above the first liquid storage pipe. The problems of mechanical pressure drifting and slow dynamic response are solved while intraocular pressure simulation precision and dynamics are guaranteed, multi-mode pressure control is achieved, practicability is high, a reliable platform is provided for medical instrument testing and pathological research, and animal experiments can be effectively replaced.

The application discloses application of a natural oyster zinc peptide product in preparation of a medicine for preventing and treating enteritis. The application verifies the function of the natural oyster zinc peptide product in preventing and treating enteritis through animal experiments, the natural oyster zinc peptide product can improve the apparent symptoms of ulcerative colitis of mice caused by DSS by regulating the oxidation stress level, relieving the inflammatory reaction, maintaining the intestinal barrier and reducing the cell apoptosis, and the natural oyster zinc peptide product can also regulate the intestinal flora and metabolic products of the intestinal flora of the enteritis mice, so that the intestinal inflammation is relieved. The natural oyster zinc peptide product used in the application has good safety, compared with common anti-inflammatory and immunity-enhancing medicines, the natural oyster zinc peptide product has lower cost and no side effect, and the effect of relieving enteritis is remarkable. Therefore, the natural oyster zinc peptide product has a good prospect in development of medicines for preventing and treating enteritis.

The utility model discloses an electronic cage card for an experimental animal rearing cage, and belongs to the technical field of electronic cage cards for animal experiments. Comprising an electronic display label information display screen and a rearing cage card, the back face of the electronic display label information display screen is fixedly connected with an electronic display label control panel and a battery bin, an LED state prompting lamp is installed in the electronic display label information display screen, and a correction hole is formed in the rearing cage card. An experimental cage buckle is fixedly connected to the back face of the rearing cage card, four penetrating holes are formed in the rearing cage card, an electronic display tag technology is combined with an existing rearing cage card, informatization of data, a rearing cage, the cage card, experimental animals and the like can be achieved, traditional management efficiency is improved, manpower, material resources and time cost are reduced, and the rearing cage card is convenient to use. Task communication is optimized. The electronic cage card can update information in time and at will, many tedious processes are omitted, and data errors are avoided.

The invention discloses application of chlorogenic acid in preparation of a product with functions of protecting intestinal mucosa and regulating intestinal flora, and belongs to the technical field of medical health care products. Animal experiments prove that the chlorogenic acid can effectively relieve intestinal mucosa barrier damage caused by hypoxia, reverse abnormal increase of alpha-diversity of intestinal bacteria and optimize the flora structure in allusion to the fact that the regulation effect of the chlorogenic acid on the intestinal mucosa damage and intestinal flora disorder induced by hypoxia is not involved in the prior art. The product is suitable for preventing and treating intestinal mucosal barrier damage and intestinal microecological imbalance caused by acute and chronic hypoxia or drug stimulation.

The utility model relates to the technical field of animal experiment equipment, and discloses a universal semi-automatic adjustment strike model device laser positioner, which comprises a base, a laser positioner and a remote control adjustment module, the top of the base is fixedly provided with an installation shell, and the inner side of the top of the installation shell is rotatably provided with an adjustment shaft; a spherical seat is fixedly connected to the adjusting shaft, a supporting rod is fixedly connected to the top of the spherical seat, a mounting seat is mounted at the top end of the supporting rod, and a protective shell is mounted on the mounting seat. According to the utility model, the light spot position of the laser positioner can be conveniently and electrically adjusted according to the specification and model of the striking model device, and the angle of the laser positioner is manually adjusted, so that the accurate adjustment of the up-down and left-right angles of a laser positioning point is realized, an experimenter can obtain higher positioning accuracy during operation, and the working efficiency is improved. Therefore, semi-automatic positioning adjustment of the laser positioner is realized, errors caused by manual operation are reduced, and positioning consistency and experiment efficiency are improved.

The utility model provides an experimental animal grabbing device. The experimental animal grabbing device comprises a box body with an inner cavity, a guide pipe arranged in the inner cavity, a first clamping assembly arranged at the tail end of the guide pipe, a second clamping assembly connected to the bottom of the box body in a sliding mode, and a first driving part for driving the second clamping assembly to slide. The first clamping assembly is used for clamping the head of an experimental sample, and a sensor for monitoring the experimental sample is arranged at the tail of the guide tube. A storage assembly is further arranged in the box body, a rubber sleeve is arranged in the storage assembly, a sleeving assembly for driving the rubber sleeve to move to the head of the experimental sample is arranged in the box body, and an elastic connecting piece connected with the rubber sleeve is arranged at the top of the box body. A first linear module is arranged at the top of the box body and drives the sleeving assembly to slide in the length direction of the box body. The second clamping assembly is used for clamping the body of the experimental sample. According to the animal experiment sample grabbing device, one-time grabbing is firm, animal samples are prevented from being damaged, the effectiveness of animal experiment sample grabbing is guaranteed, and the personal safety of operators is effectively protected.

The application discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and cantharides polypeptide at a mass ratio of 1:1. Animal experiments show that, when meat chickens are fed with 60 mg of the biological additive per 1 kg of basic feed, the growth of the meat chickens can be promoted, and more importantly, the feather development of the meat chickens can be significantly and synergistically promoted, and the length of the main wing feathers is obviously increased. Cytological studies further prove that the biological additive can synergistically promote the proliferation of poultry hair follicle epidermal stem cells, and the effect is better than that of an EGF positive control. Therefore, the biological additive developed by the application is safe, efficient and green, effectively solves the problems of drug residues and drug resistance possibly caused by traditional chemical additives, provides a sustainable solution for poultry breeding, and has important economic and application values.

The utility model provides a breathable comfortable rearing cage, which relates to the field of animal experiments and comprises a cage body, an opening is arranged at the upper end of the cage body, a turnover cover plate is mounted and connected at the opening, a fastener is arranged at one end of the turnover cover plate, an air inlet is arranged on the side wall of one end of the cage body, and an air outlet is arranged on the side wall of one end of the cage body. And one end of the external expansion pipe is fixedly connected with the air inlet. The small fan is started to enable external air to flow into the external expansion pipe, the air can be uniformly heated to a proper temperature by the aid of the electric heating piece, the heat conduction head and the porous heat conduction sheet plate, constant-temperature preheating treatment can be performed on the flowing-in air, the feeding cage effectively adapts to various temperature environments, comfortable and stable temperature conditions are provided for animals in the feeding cage, and the feeding cage is convenient to use. Healthy growth and survival of animals are facilitated, the problems of diseases or poor growth and the like caused by inappropriate temperature are reduced, and the practicability and applicability of the rearing cage are improved.

The application relates to a mouse metabolic behavior detection intervention device and an intervention method, and belongs to the technical field of animal experiment equipment. The device comprises a shell, an intervention cabin and a metabolism cabin are arranged in the shell, the intervention cabin comprises a cabin body located in the shell, an inducer for guiding experimental mice to spontaneously enter the cabin body is arranged at one end of the cabin body away from an entrance end of the cabin body, a self-motion mechanism is located between the inducer and the entrance end of the cabin body, is used for carrying the mice and generating reciprocating motion in response to spontaneous movement of the mice along the length direction of the cabin body, a monitoring mechanism is located at the periphery of the cabin body, is used for monitoring the position, state and motion track of the experimental mice in the cabin body, and a sealing mechanism is located at the entrance end of the cabin body and is used for opening or closing the entrance of the cabin body according to a detection signal of the monitoring unit. The mice spontaneously enter the intervention cabin through the inducer and autonomously run on the conveying belt, traditional forced motion means such as electric shock, a push rod and high-pressure airflow are completely abandoned, and the accuracy of monitoring data is improved.