Food-grade aspergillus niger strain and application of strain in zearalenone degradation

A technology of zearalenone and Aspergillus niger, which is applied in the field of Aspergillus niger, can solve the problems of nutrient composition destruction, time-consuming and labor-consuming, loss of animal feed nutrients, etc., and achieve the effect of reducing the content

Active Publication Date: 2014-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical detoxification method will also cause the loss of animal feed nutrients while removing the zearalenone toxin; and although the chemical detoxification method can remove some grain feeds with severe mildew, it is time-consuming and labor-intensive, and it is harmful to the environment. Nutrients cause great damage, and may also produce some chemicals that are harmful to animal health. It is worth noting that the EU does not allow the application of chemical methods to eliminate mycotoxins in food production.

Method used

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  • Food-grade aspergillus niger strain and application of strain in zearalenone degradation
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  • Food-grade aspergillus niger strain and application of strain in zearalenone degradation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the screening method of Aspergillus niger in sauce unstrained spirits

[0019] The non-toxin-producing filamentous fungi in the fermented sauce were qualitatively isolated from the fermented sauce with β-cyclodextrin-containing PDA medium, and then the toxin-producing ability of the isolated strains was quantitatively analyzed by ELISA method, and finally Screen out non-toxigenic molds. Re-screen the toxin-producing bacteria that can inhibit Aspergillus flavus, and screen out the strains that can prevent and control mycotoxins.

[0020] Through the observation of colony characteristics and individual morphology, the 18S rDNA of the strain was compared with the GenBank database ( http: / / blast.ncbi.nlm.nih.gov / Blast.cgi ) and found that its homology with Aspergillus niger was 100%. Combined with the morphological characteristics and molecular biological identification of the strain, it can be determined that the strain is Aspergillus niger. This strain w...

Embodiment 2

[0021] Embodiment 2: Degradation of Zearalenone by Aspergillus niger

[0022] The isolated strain FS-Z1 was inoculated into 60ml of sterile PDB medium at a 2% inoculum size, cultured on a shaker at 28°C and 150rpm for 5 days. Add zearalenone to the fermentation broth to make the final concentration reach 2ppm, use sterile PDB culture fluid plus zearalenone as a blank control, and after culturing for 48 hours, draw 2ml of liquid from two groups of samples and add 4ml of trichloro Methane, extracted 3 times, dried with nitrogen, then redissolved with 1ml of mobile phase [acetonitrile: water = 50:50 (v / v)], passed through a 0.22μm organic filter membrane and carried out by high performance liquid chromatography (HPLC) detection.

[0023] The chromatographic detection conditions are: chromatographic column: C18 column 6mm×150mm×5um; mobile phase: acetonitrile:water=50:50; detection temperature: 25°C; flow rate: 1ml / min; detection wavelength: excitation wavelength: 274nm; emission...

Embodiment 3

[0025] Embodiment 3: Aspergillus niger FS-Z1 is to the control of the corn steep liquor of zearalenone toxin pollution

[0026] The isolated strain FS-Z1 was inoculated into 60ml of sterile PDB medium at a 2% inoculum size, cultured on a shaker at 28°C and 150rpm for 5 days. Filter the mycelium of Aspergillus niger with four layers of sterile gauze to obtain the fermentation liquid of Aspergillus niger. Mix the fermented liquid with corn steep liquor [1:1 (v / v)] and shake evenly, mix the sterile PDB medium with equal volume of corn steep liquor as a blank control, culture at 28°C and 150rpm for 48h, filter through glass fiber filter paper Filter 1 to 2 times until the filtrate is clear, extract zearalenone in the sample according to the above method, and detect the content of residual zearalenone toxin by high performance liquid chromatography.

[0027] Measured by high-performance liquid chromatography, it was found that the concentration of zearalenone in 0h corn steep liqu...

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Abstract

The invention discloses a food-grade aspergillus niger strain FS-Z1 which is separated out from sauce grains and capable of preventing and treating zearalenone toxin. The collection number of the food-grade aspergillus niger strain FS-Z1 is CCTCC NO: M 2013703. The strain has excellent degradation effect on the zearalenone and the degradation rate reaches 89.56%. Besides, animal experiments in rats verify that the zearalenone in the corn steep liquor degraded by using an aspergillus niger fermentation liquor has no toxicity.

Description

technical field [0001] The invention relates to a strain of Aspergillus niger, in particular to a food-grade bacterial strain capable of preventing and treating zearalenone toxin isolated from fermented soy sauce. Background technique [0002] Zearalenone (ZEA) is an estrogen-like mycotoxin produced by Fusarium fungus. It is the most widely polluted Fusarium toxin in the world. heavily polluted. In China, poor grain harvesting conditions and oil processing have aggravated the pollution of ZEA. Low-quality corn and by-products of grain processing, especially the widespread use of unconventional raw materials, have caused the pollution of mycotoxins such as ZEA to far exceed foreign countries. The primary toxicity of ZEA and its metabolites has been attributed to their estrogen and anabolic activity. A large number of studies have confirmed that ZEA and its metabolites can cause mycotoxin poisoning in many animals, affect the development of internal organs, and can also caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/00A23L1/015C12R1/685A23K10/00A23L5/20
Inventor 孙秀兰何兴兴徐丹张银志
Owner JIANGNAN UNIV
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