123 results about "Aspergillus mottae" patented technology

The invention belongs to the field of gene engineering, and discloses a recombination gene and a method for increasing aspergillus niger expressed saccharifying enzyme. The method for increasing aspergillus niger expressed saccharifying enzyme disclosed by the invention is as follows: by means of a homologous recombination method, a saccharifying enzyme expression cassette and selectable marker genes are integrated in an aspergillus niger An12g08830 gene locus in a targeted manner; then, saccharifying enzyme is obtained by recombining aspergillus niger through conventional resistance screening and fermentation cultivating. According to the invention, after the saccharifying enzyme expression cassette is integrated in the aspergillus niger An12g08830 gene locus in a targeted manner through the improved homologous recombination method, the conversion efficiency and the saccharifying enzyme expressing activity are obviously increased; furthermore, a lot of processes for screening converters are omitted.

Fungal glucoamylases from Aspergillus fumigatus - expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

The invention discloses a method for enhancing glucose conversion rate of an Aspergillus niger saccharifying enzyme. The composite of an agdB-gene-inactivated Aspergillus-niger-secreted saccharifying enzyme and a debranching enzyme is utilized to convert starch sugar. The alpha glucosaccharase agdB gene is inactivated to lower the saccharifying enzyme fermentation activity of Aspergillus niger, and the saccharifying enzyme can be directly compounded with the debranching enzyme to convert glucose, thereby obviously enhancing the DX value.

The invention discloses aspergillus niger and a method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger. The aspergillus niger FOS-0620 is an aerobe, has black spores and milk-white hyphae, and is collected in China General Microbiological Culture Collection Centre (CGMCC for short) on September 28, 2012. The method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger comprises the following steps of: (1) preparing aspergillus niger whole-cells by virtue of the aspergillus niger FOS-0620 according to claim 1; and (2) catalytically producing fructo-oligosaccharide by virtue of the aspergillus niger whole-cells. The product obtained by the method disclosed by the invention is detected that the content (occupying total solids) of the fructo-oligosaccharide is not less than 50% via high-pressure liquid chromatography; and the method disclosed by the invention is simple in process, convenient to operate, high in enzymatic activity, high in conversion efficiency, and important in industrial value.

The invention relates to preparation and application of aspergillus niger used for phosphate degrading, potassium degrading and cellulose degradation and a microbial agent of the aspergillus niger. The CS-1strain has the ability to degrade phosphorus and potassium and degrade cellulose efficiently; the CS-strain 1 can play a role in phosphate degrading, potassium degrading and cellulose degradation when the roots of wheat, cucumber, apple and other crops are stable; the microbial agent prepared from the strain can significantly promote the growth of the crops, can be applied to the productionof microbial fertilizers. The aspergillus niger has important significance in effectively reducing the use amount of chemical fertilizers, enhancing the soil fertility, increasing the crop yield and promoting the direct return of straw to the field.

The invention discloses an aspergillus niger alpha-glucosidase aglu<OP> and a high-efficiency expression method of the aspergillus niger alpha-glucosidase aglu<OP> in pichia pastoris. The nucleotide sequence of the optimized alpha-glucosidase gene is shown in SEQ ID No.1. The gene is constructed into a pichia pastoris expression vector, and the pichia pastoris expression vector and disulphide bond isomerase are transformed into the pichia pastoris for co-expression, and the enzyme activity of the alpha-glucosidase gene can be up to 10.1U/mL after induction is carried out for 110 hours in a 3L tank, and is 2.9 times of the enzyme cavity before optimization. The optimized alpha-glucosidase gene can be used for industrial production of the alpha-glucosidase.

The invention discloses lactic acid fermented soybean paste. The soybean paste is prepared from, by weight, 400-430 parts of soy beans, 130-150 parts of corn germ, 10-12 parts of lactobacillus bulgaricus, 10-15 parts of aspergillus oryzae, 7-10 parts of aspergillus niger and 7-8 parts of active dry yeast. A preparation method of the lactic acid fermented soybean paste comprises the steps of firstly, using lactic acid for fermenting part of the soy beans, obtaining lactic acid soy beans which are richer in nutrition, and then fabricating the soybean paste with the lactic acid soy beans and the remaining soy beans. The soybean paste is good in taste and rich in nutrition, extracting solutions of traditional Chinese medicinal materials such as mint, kudzu vine roots, cassia twigs and momordica grosvenori are added, a certain heat-clearing and detoxifying effect is achieved, and illness caused by excessive internal heat can be avoided when the soybean paste is eaten.

The invention provides a method for producing ethyl alcohol through crop stalks by use of recombinant zymomonas mobilis. According to the method, the crop stalks are used as a base material, aspergillus niger and trichoderma are mixed and fermented to produce a crude enzyme solution containing cellulase, the crop stalks are processed through the crude enzyme solution to enable cellulose in the crop stalks to be decomposed to produce reducing sugar, and finally recombinant zymomonas mobilis is used for fermenting the enzymatically-decomposed reducing sugar to produce ethyl alcohol. In the method, fermentation products of aspergillus niger and trichoderma are mixed according to the ratio of 1: 1 to obtain the cellulase crude enzyme solution, so that the product feedback inhibition is effectively controlled, and the cellulase crude enzyme with higher activity and yield is obtained. As zymomonas mobilis is inoculated in the enzymatically-decomposed fermentation liquor containing reducing sugar, the ethyl alcohol production efficiency through the crop stalks by use of zymomonas mobilis is greatly improved, and defects that the use range of a zymomonas mobilis substrate is narrow, and the crop stalks cannot be effectively utilized are overcome. According to the method, the conversion rate of ethyl alcohol through the crop stalks is 5%, that is, 100 g of the crop stalks can produce 5 g of ethyl alcohol.

The invention discloses an acid protease containing compound enzyme of soy sauce. The acid protease containing compound enzyme of the soy sauce is prepared by taking proteases containing high-activity acid proteases and other food-grade enzymic preparations as main materials and scientifically compounding the main materials with high-activity acid proteases, mould cultures, plant extracts containing various plant enzyme systems, spice extracts, novasil, a protective agent, an activating agent, an antioxidant and the like, wherein the mould cultures have functions of inhibition of growth and propagation of aspergillus flavus and escherichia coli and decomposition of aflatoxin; the spice extracts are effective in inhibition of aspergillus flavus, bacillus subtilis, staphylococcus aureus and escherichia coli and not effective in inhibition of aspergillus niger and aspergillus oryzae and have functions of aroma enhancement and flavoring; the protective agent is capable of evidently improving storage stability of the compound enzyme of the soy sauce. The prepared compound enzyme of the soy sauce has the advantages of complete enzyme system, high enzyme activity, high stability, high potency and high food safety. The soy sauce yield can be remarkably increased by 24%, and a total nitrogen utilization rate and an amino acid generation rate of brew sauce are increased by 16.5% and 18.4% respectively.

The invention relates to an Aspergillus niger and a cultivation method and application thereof. The Aspergillus niger BLCY-02 is collected in the China General Microbiological Culture Collection Center on 15th, July in 2014 and has the collection number of CGMCC No.9449. The invention also relates to a cultivation method and application of the Aspergillus niger.

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

The invention belongs to the technical field of bioengineering and discloses a method for increasing yield of itaconic acid produced by fermentation of aspergillus terreus. By adoption of acid-induced promoters derived from aspergillus niger, lateral respiratory chain protein AOX of the aspergillus niger is introduced into the aspergillus terreus to improve supply of an intracellular NAD+ bank, and accordingly glycolytic pathway can be enhanced to realize increase of itaconic acid yield. The itaconic acid yield of recombinant aspergillus terreus strains obtained by the method is increased by about 20% to reach 60g/L. The method provides a fresh idea for itaconic acid production.

The present invention discloses high xylanase yield Aspergillus niger and an application thereof, wherein the Aspergillus niger SM24/a has the preservation number of CGMCC No.8671. According to the present invention, the Aspergillus niger SM24/a can produce xylanase providing good tolerance for ethanol, acetic acid, furfural, vanillin, ferulic acid and a mixture of the above materials, the xylanase production activity of the strain is high, the solid fermentation enzyme activity of the xylanase can be up to 10801 IU/g (catalysis production of 1 [mu]mol of xylose within 1 min is defined as an enzyme unit), the optimum pH value is 5.6, the optimum reaction temperature is 37 DEG C, and the 50 DEG C enzyme activity remaining rate is 79.01%; and 5-hydroxymethyl furfural provides an inhibition effect for the enzyme, and the concentration and the enzyme inhibition rate present a certain linear relationship when the 5-hydroxymethyl furfural exists in a separate or mixed manner, such that the xylanase can further be used as the qualitative even quantitative indicator of the 5-hydroxymethyl furfural concentration.

The invention discloses a strain of Aspergillus oryzae ZA116. The Aspergillus oryzae ZA116 is preserved in Guangdong Microbiological Culture Collection Center on November 15, 2018, the preservation number is GDMCC 60479, and the preservation address is 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The invention further provides application of the Aspergillus oryzae ZA116 in foodfermentation or koji making or soy sauce making. The strain ZA116 obtained through mutagenesis screening is high in autolysis rate in the fermentation stage, high in glutamic acid content in soy sauceand clearer in crude oil; the Aspergillus oryzae ZA116 with the high autolysis rate is adopted for improving the quality of soy sauce by fully releasing enzymes and nutrients in bacteria, the changeof an existing production process and replacement of production equipment are not involved, the production cost is low, and industrialized popularization is facilitated.

The invention discloses an immobilization method of Aspergillus niger. According to the immobilization method, a pretreated fibrous material is used as an immobilization medium, the Aspergillus niger is activated and then cultured in a fermentation container containing the immobilized medium, and the strain is adsorbed on the fibrous material in the culturing process. When an airlift-type fiber bed reactor with polyester fiber as the immobilization medium is used for continuously producing citric acid for 15 batches, the acid producing level is not reduced and the fermentation process is stable. Ultimately, the citric acid yield is 88.65g/L, the fermentation rate is 1.06g/L/h, and the yield rate (citric acid yield/glucose consumption) is 93.3% (w/w). Compared with free fermentation, the yield of immobilized fermentation is obviously increased, the fermentation period is shortened, the number of mycelia in a fermentation broth is obviously reduced, and the viscosity is decreased therewith, so that separation of a product and the mycelia at a later period is facilitated.

The invention aims at providing a trichoderma reesei bacterial strain for producing saccharifying enzyme. The trichoderma reesei bacterial strain is trichoderma reesei HDGAU-1 (Trichoderma reesei HDGAU-1) with a preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013584. The trichoderma reesei bacterial strain disclosed by the invention can efficiently express the saccharifying enzyme, and fermentation enzyme activity is as high as 4000 U/ml which is improved by 54% in comparison with that before mutation, and protein amount exceeds 2.1 g/L which is improved by about 50% in comparison with that before mutation. According to the invention, production cost of the saccharifying enzyme can be greatly lowered by utilizing the trichoderma reesei bacterial strain to produce the saccharifying enzyme, and wide application of the saccharifying enzyme is facilitated. Moreover, the saccharifying enzyme recombinant and expressed by the trichoderma reesei bacterial strain disclosed by the invention has an optimum acting temperature of 70 DEG C and an optimum acting pH of 5.5, has catalytic efficiency higher than that of the common saccharifying enzyme from aspergillus niger at present, is more high temperature resistant, can effectively shorten saccharifying time and can lower production cost.

The invention discloses a method for preparing a yeast culture through distillers' grains and an application of the yeast culture. The method comprises the following steps of S1, supplementing a carbon source to distillers' grains to obtain pretreated distillers' grains, inoculating mixed bacterium liquid to the pretreated distillers' grains, adding mixed enzyme liquid, preparing a fermentation base material, and performing fermentation culture for 96-120h; and S2, after fermentation is completed, performing inoculation with bacillus subtilis, continuing to perform fermentation culture for 24h, and performing baking, crushing and screening so as to obtain the yeast culture, wherein the mixed bacterium liquid comprises 3 strains of saccharomyces cerevisia, lactic acid bacteria and aspergillus niger, and the mixed enzyme liquid comprises liquefying amylase, glucoamylase, phytase and proteases. According to the method disclosed by the invention, the distillers' grains are used as substrates, the mixed bacterium liquid and the mixed enzyme liquid are added for heap fermentation, so that the yeast culture is obtained; and the distillers' grains are turned into wealth from waste, and besides, a feed rich in nutrient components is provided for animals, so that improvement of the immunity of the animals can be facilitated.

The invention relates to an aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as a culture method and application thereof. An aspergillus oryzae FS4 strain is collected in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2014, with the collection number of CGMCC No. 9087 and the address of Institute of Microbiology, Chinese Academy of Sciences, Building 3, NO.1 Beichen West Road, Chaoyang District, Beijing. The invention further relates to the culture method and application of the aspergillus oryzae strain. The aspergillus oryzae FS4 strain obtained by screening has the advantages of simple culture, stable genetic nature, high synthesis efficiency and stable enzyme activity, can be applied to the field of synthesis of levan type oligofructoses and the like by a modern bio-engineering technical enzyme method, and further has broad application prospects.

The invention discloses a method for manufacturing lactobacillus fermented soybean rich in gamma-aminobutyric acid. The method includes: sequentially subjecting soybeans to cleaning, soaking, draining and steaming, and performing bacterial powder inoculation for starter propagation and fermentation to obtain fermented soybean, wherein before starter propagation, mixed bacterial powder of aspergillus oryzae and aspergillus niger is adopted for inoculation in a mass weight of 1.5-4:1, and after starter propagation, lactobacillus plantarum containing bacteria liquor is adopted for inoculation prior to fermentation. By adoption of the mixed bacterial powder of aspergillus oryzae and aspergillus niger for starter propagation, adoption of the lactobacillus plantarum containing bacteria liquor for fermentation, and coordination of appropriate starter propagation and fermentation processes, protease activity of finished starter, content of amino nitrogen generated in fermentation and content of gamma-aminobutyric acid are increased remarkably, and finished products of fermented soybean are healthier and greater in quality and flavor. In addition, the preparation process is simple, a fermentation period is greatly shortened, and production cost is reduced.

The invention provides a new bacterial strain-Aspergillus oryzae 2013-DP1, and applications in preparation of feed additives through microbial fermentation. The strain is preserved in China Center for Type Culture Collection. The address is Wuhan University, Wuhan, China. The postcode is 430072. The preservation date is June 21th, 2013. The preservation number is CCTCC No: M2013276. Through fermentation and enzymatic hydrolysis conversion of low-value cellulose substances of straws and the like, the strain raises the protein content properly, raises nutritive values, can promote development of feed sources, raises the utilization rate of waste biological resources, and has obvious economic and ecological significances.

The invention discloses an Aspergillus niger strain and an application thereof. The strain is named Aspergillus niger ZJUQH2012147, which is collected in China General Microbiological Culture Collection Center (CGMCC) on May 23, 2012 with the collection number of CGMCC No. 6152. The invention further discloses a method for producing feruloyl esterase by using the strain, and the method comprises the following steps: (1) inoculating the Aspergillus niger ZJUQH201217 into a seed culture medium for propagation culture to obtain seed liquid; and (2) inoculating the seed liquid into a fermentationculture medium for fermentation culture. The method for producing feruloyl esterase by using the Aspergillus niger strain disclosed by the invention has high activity and is easy to realize industrial production of the feruloyl esterase. The process for producing the feruloyl esterase disclosed by the invention adopts soybean meal, wheat bran and other crude raw materials as fermentation raw materials, pretreatment of the crude raw materials is not required, and the production cost is low.

The invention discloses aspergillus niger for largely producing xylanase and application thereof. The collection number of the Aspergillus niger SM751 is CGMCC No. 8670. The xylanase generated by the Aspergillus niger SM751 is activated in enzyme activity under the action of mixing of multiple inhibitors; compared with blank control, the maximal activation rate of the enzyme activity achieves 33.43%. The Aspergillus niger SM751 provided by the invention can be used for largely producing xylanase which has high enzyme activity and can tolerate the pretreated fermented inhibitors, thereby having wide application in the field of lignocellulose enzymolysis.

The invention discloses an aspergillus niger hydrolytic enzyme AnCu3. The amino acid sequence of the aspergillus niger hydrolytic enzyme AnCu3 is shown as SEQID No.1. The invention further discloses an application of the aspergillus niger hydrolytic enzyme AnCu3 to catalyzing and hydrolyzing of plasticizer dimethyl glycol phthalate (2-ethylhexyl) ester (DEHP) in a water phase system. The inventionprovides an enzyme which can catalyze and hydrolyze the dimethyl glycol phthalate (2-ethylhexyl) ester, and a coding gene for the first time, and a new way is provided for hydrolyzing of the dimethylglycol phthalate ( 2-ethylhexyl) ester.