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Immobilization method of Aspergillus niger

An Aspergillus niger, immobilized medium technology, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve problems such as affecting fermentation performance, and achieve reduced fermentation time and good transmission. The effect of quality performance, reducing the loss of cells

Active Publication Date: 2017-08-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using porous fiber material as the fixing material not only has stable process, non-toxic, cheap and easy to obtain, good adsorption effect, high reuse rate, but also has a good protective effect on cell activity. Many, it is easy to overgrow on the surface of the fiber bed, and finally wrap the fiber bed together, which seriously affects the fermentation performance, so few people use the adsorption method as a fixation method for Aspergillus niger

Method used

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  • Immobilization method of Aspergillus niger
  • Immobilization method of Aspergillus niger
  • Immobilization method of Aspergillus niger

Examples

Experimental program
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Effect test

Embodiment approach

[0032] (1) Wash the fiber material in deionized water for 1-3 hours, then soak the cleaned material in 10-50% (mass fraction) NaOH for 1-6 hours, and then wash the material in deionized water for 1 hour ~3h, until the NaOH is cleaned and the surface of the carrier is neutral;

[0033] (2) Place the porous fiber material treated in step (1) in 0.1-10% (volume fraction) aqueous solution of silane coupling agent, soak for 10-60min, then place the porous fiber material in deionized water to fully rinse Then put it in 120 ℃ drying, save for future use.

[0034]The silane coupling agent is vinyltriethoxysilane, vinyltrimethoxysilane, 1,2-bistrimethoxysilylethane, methylphenyldimethoxysilane, methyltriacetyl Any one or more of oxysilanes.

Embodiment 1

[0035] The preparation of embodiment 1 porous fibrous material immobilization medium

[0036] (1) Wash the polyester fiber in deionized water for 1 h, then soak it in 25% (mass fraction) NaOH for 3 h, then wash the polyester fiber in deionized water for 3 h until the NaOH is cleaned, and the surface of the carrier is neutral;

[0037] (2) Soak the polyester fiber in 6% (volume fraction) vinyltriethoxysilane aqueous solution for 30 minutes, then place the polyester fiber in deionized water for full rinsing, dry at 120°C, and store at 4°C for later use .

Embodiment 2

[0038] The preparation of embodiment 2 porous fibrous material immobilization medium

[0039] (1) Wash the polyethylene carrier in deionized water for 1.5h, then soak it in 25% (mass fraction) NaOH for 2h, then wash the polyethylene in deionized water for 2h until the NaOH is cleaned, and the surface of the carrier is neutral;

[0040] (2) Soak the polyethylene in a 3% (volume fraction) vinyltrimethoxysilane aqueous solution for 20 minutes, then rinse the polyethylene fully in deionized water, dry it at 120°C, and store it at 4°C for later use.

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Abstract

The invention discloses an immobilization method of Aspergillus niger. According to the immobilization method, a pretreated fibrous material is used as an immobilization medium, the Aspergillus niger is activated and then cultured in a fermentation container containing the immobilized medium, and the strain is adsorbed on the fibrous material in the culturing process. When an airlift-type fiber bed reactor with polyester fiber as the immobilization medium is used for continuously producing citric acid for 15 batches, the acid producing level is not reduced and the fermentation process is stable. Ultimately, the citric acid yield is 88.65g / L, the fermentation rate is 1.06g / L / h, and the yield rate (citric acid yield / glucose consumption) is 93.3% (w / w). Compared with free fermentation, the yield of immobilized fermentation is obviously increased, the fermentation period is shortened, the number of mycelia in a fermentation broth is obviously reduced, and the viscosity is decreased therewith, so that separation of a product and the mycelia at a later period is facilitated.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to an immobilization method of Aspergillus niger. Background technique [0002] Citric acid, also known as citric acid, chemical name 2-hydroxypropane-1,2,3-tricarboxylic acid, is an important organic acid, colorless crystal, often contains a molecule of crystal water, odorless, strong Sour taste, soluble in water. The physical properties of citric acid are generally colorless transparent or translucent crystals, or granular and granular powders, which are easily soluble in water, and the solubility increases with the increase of temperature. Citric acid is a tricarboxylic acid compound and thus has similar properties to other carboxylic acids. When heated to 175°C, it will decompose to produce carbon dioxide and water, leaving some white crystals. Citric acid is a strong organic acid with 3 H + It can be ionized; it can be decomposed into various products...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N11/08C12N11/12C12N11/14C12R1/685
CPCC12N11/02C12N11/08C12N11/12C12N11/14C12R2001/685C12N1/145
Inventor 应汉杰余斌陈勇张欣孙文俊赵南
Owner NANJING UNIV OF TECH
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