The invention discloses a feed for promoting egg laying of laying hens and a preparation method thereof. The feed comprises the following components by weight: 62-65 parts of beer yeast slurry, 2.4-2.7 parts of slaked lime, 170-175 parts of soybean meal, 19-24 parts of rice bran meal, 32-36 parts of onion residue, 15-17 parts of pear seed powder, 4-5 parts of apple vinegar, 23-26 parts of celery powder, 14-18 parts of shrimp powder, 34-37 parts of mung bean dregs, 23-26 parts of buckwheat bran, 3-4 parts of peanut butter, 2-3 parts of sea fish oil, 5-6 parts of perilla leaf, 3-4-4 parts of motherwort, 3-4 parts of Epimedium, 2-3 parts of red dates skin, 14-18 parts of plasma protein powder, 2-3 parts of mango juice, 2-3 parts of medicinal fermented mass and1-2 parts of cashew oil. The feed has rich nutrition, good palatability, good feeding attraction, delicate fragrance and good taste, can promote the hen to lay eggs, promote follicular development of hens, improve the reproductive performance of hens, improve the rate of egg production, improve egg quality, and increase egg nutrition.