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A method for extracting astaxanthin, yeast extract and glucan from yeast

A technology of yeast extract and astaxanthin, which is applied in food science, organic chemistry, etc., can solve the problems of unfavorable industrial production and application of products, hindering large-scale industrial production, and large consumption of carbon dioxide, so as to avoid adverse effects and facilitate The effect of obtaining and producing low cost

Active Publication Date: 2017-04-19
广东雅琪生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these solvents are toxic reagents, and the price is relatively high. There are safety hazards in the production process and in the product, which is not conducive to the industrial production and application of the product.
Although supercritical carbon dioxide extraction can effectively extract astaxanthin and avoid its degradation to the greatest extent, it requires high equipment, is expensive, and consumes a large amount of carbon dioxide. The content of astaxanthin in the extract is low, which hinders its large-scale production. scale industrial production
[0007] There are no reports on the efficient simultaneous production of multiple products (i.e., astaxanthin, yeast extract, and dextran) and the efficient extraction of astaxanthin from yeast and the preparation of yeast extract and dextran using the same yeast species craft

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The groping of embodiment 1 astaxanthin extraction conditions

[0045] 1. Design three test groups with different yeast cell contents

[0046] (1) The volume of the reaction system is 100mL, the wet cells of Phaffia rhodozyma (ATCC66270) are respectively 10g, 15g and 20g (wherein the dry cells of Phaffia rhodozyma are 12.25% of its wet cell mass), cellulase ( Celluclast 1.5L, Novozymes, Denmark) solution 4mL, add deionized water, use 3M hydrochloric acid to adjust the pH value to about 4.5, then use deionized water to adjust the volume to 100mL; react at 50°C for 6h at a stirring speed of 150rpm, Boil for 3 minutes to inactivate the enzyme activity.

[0047] (2) After the reaction, centrifuge at 10,000 rpm for 10 min, collect the precipitate, and wash the precipitate with deionized water.

[0048] (3) Add 100mL of edible ethanol to the yeast after enzymolysis, oscillate on a shaker with a rotating speed of 150rpm and extract in the dark for 60min, centrifuge at 10000r...

Embodiment 2

[0069] The groping of embodiment 2 yeast extract preparation conditions

[0070] 1. Design three test groups with different dosages of yeast extract enzymes

[0071] (1) The volume of the reaction system is 1 L, containing 100 g of wet cells of Phaffia rhodozyma (ATCC66270) and 60 mL of cellulase, the pH value is adjusted to about 4.5 with 3M hydrochloric acid, and the deionized water is constant to volume; at a stirring speed of 150 rpm, React at 50°C for 6 hours, boil for 3 minutes to inactivate the enzyme activity.

[0072] (2) After the reaction, centrifuge at 10,000 rpm for 10 min, collect the precipitate, and wash the precipitate with deionized water.

[0073] (3) Add ethanol 60mL / g dry cells to the yeast after enzymolysis, oscillate on a shaking table with a rotation speed of 150rpm and extract in the dark for 60min, centrifuge at 10000rpm for 10min, collect the supernatant and precipitate respectively; measure the astaxanthin in the supernatant, The extraction rate o...

Embodiment 3

[0082]The groping of embodiment 3 dextran preparation conditions

[0083] 1. Design three test groups with different concentrations of lye

[0084] (1) The volume of the reaction system is 1L, containing 100g of Phaffia rhodozyma (ATCC66270) cells and 60mL of cellulase, the pH value is adjusted to about 4.5 with 3M hydrochloric acid, and the volume is constant with deionized water; React at ℃ for 6h, boil for 3min to inactivate the enzyme activity.

[0085] (2) After the reaction, centrifuge at 10,000 rpm for 10 min to wash the precipitate.

[0086] (3) Add edible ethanol 60mL / g dry cells to the yeast after enzymolysis, oscillate on a shaker at 150rpm to extract in the dark for 60min, centrifuge at 10000rpm for 10min, collect the supernatant, and obtain an astaxanthin extraction rate of 91.93%.

[0087] (4) Add deionized water to the bacterial precipitate after extracting the pigment according to the solid-to-liquid ratio of 10%, add 3% potassium chloride and 3% yeast extrac...

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Abstract

The present invention discloses a method for extracting astaxanthin yeast, yeast extract and glucan from yeast. According to the method, cellulose is used for enzymolysis of yeast, then an organic solvent is added for dark shaking extraction and solid-liquid separation, and the resulting liquid is astaxanthin solution, obtained solid is mixed evenly with water, yeast extraction enzyme and inorganic salts are added for reacting at 55 DEG C, the yeast extract is obtained by solid-liquid separation and drying of obtained liquid, obtained solid is mixed evenly with alkaline liquor for reacting and solid-liquid separation, and obtained solid is washed and dried to obtain the yeast glucan. The method is short in time and mild in conditions; astaxanthin extraction rate is high, and the yeast extract and the glucan and other a plurality of products can be obtained, so that Phaffia rhodozyma utilization value can be improved, economic efficiency is increased, and a solid foundation is lay for industrial production.

Description

technical field [0001] The invention belongs to the extraction and development of natural products, in particular to a method for extracting astaxanthin, yeast extract and glucan from yeast. Background technique [0002] Phaffia rhodozym, an obligate aerobic microorganism capable of synthesizing a variety of carotenoids. Wild Phaffia rhodozyme can produce more than 10 kinds of carotenoids, mainly astaxanthin. Therefore, Phaffia rhodozyme is currently the most commonly used strain for the production of astaxanthin by microbial fermentation. However, due to the low yield of astaxanthin prepared by the current biological method, the production cost is high, resulting in low economic benefits. Therefore, there is an urgent need to find a method for comprehensively developing Phaffia rhodozyme to produce high value-added products, increase economic benefits, reduce production costs, and enable better industrial application of Phaffia rhodozyme. Because the total nitrogen conte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24A23L33/145C08B37/02
CPCC07C403/24C08B37/0009
Inventor 朱明军周玲燕
Owner 广东雅琪生物科技股份有限公司
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