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Tissue culture method for Vaccinium bracteatum Thunb

A technology of tissue culture and black rice tree, applied in the field of plant cultivation, can solve problems such as difficulty in breeding black rice tree

Active Publication Date: 2013-11-27
江苏九久环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for tissue culture of black rice tree with high multiplication rate and fast growth rate for the difficult problem of black rice tree propagation

Method used

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  • Tissue culture method for Vaccinium bracteatum Thunb
  • Tissue culture method for Vaccinium bracteatum Thunb
  • Tissue culture method for Vaccinium bracteatum Thunb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1: the quick seedling raising method of black rice tree tissue culture, concrete steps are as follows:

[0099](1) Take the strong branches with axillary buds of the black rice tree in the same year and remove the leaves as explants. First soak in 1wt% washing powder aqueous solution for 15 minutes, then gently scrub with a soft brush, and then put them under running water. Rinse for 2 hours, then soak in 70% alcohol for 60s on the ultra-clean workbench, rinse with sterile water for 3 times; then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 8 times; blot the surface moisture Finally, cut the stem segment into a small segment with a length of 1 cm and 1 axillary bud.

[0100] (2) Inoculate the stem segments treated in step (1) into the induction medium, which is added with 2.0mg / L of ZT, 0.1mg / L of IBA and 0.2mg / L of WPM conventional medium at the same time. L's GA 3 ;Cultivate for 30 days under the conditions of tempera...

Embodiment 2

[0103] Embodiment 2: the quick seedling raising method of black rice tree tissue culture, concrete steps are as follows:

[0104] (1) Take the strong branches with axillary buds of the black rice tree in the same year and remove the leaves as explants. First soak in 1wt% washing powder aqueous solution for 12 minutes, then gently scrub with a soft brush, and then put them under running water. Rinse for 3 hours, then soak in 70% alcohol for 50s on the ultra-clean workbench, rinse with sterile water for 4 times; then disinfect with 0.1% mercury chloride for 15 minutes, rinse with sterile water for 6 times; blot the surface moisture Finally, the stem segment is cut into a small segment with a length of 1.3 cm and 1 axillary bud.

[0105] (2) Inoculate the stem segment treated in step (1) into the induction medium, which is added with 1.5mg / L ZT, 0.3mg / L IBA and 0.15mg / L L's GA 3 ;Cultivate for 35 days under the conditions of temperature 25°C, light intensity 2000Lux, and light ...

Embodiment 3

[0108] Embodiment 3: the fast seedling raising method of black rice tree tissue culture, concrete steps are as follows:

[0109] (1) Take the strong branches with axillary buds of the black rice tree in the same year and remove the leaves as explants. First soak in 1wt% washing powder aqueous solution for 15 minutes, then gently scrub with a soft brush, and then put them under running water. Rinse for 4 hours, then soak in 70% alcohol for 55s on the ultra-clean workbench, rinse with sterile water for 4 times; then disinfect with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 6 times; blot the surface moisture Finally, the stem segment is cut into a small segment with a length of 1.5 cm and 1 axillary bud.

[0110] (2) Inoculate the stem segment treated in step (1) into the induction medium, which is added with 1.0mg / L of ZT, 0.4mg / L of IBA and 0.15mg / L of WPM conventional medium at the same time. L's GA 3 ;Cultivate for 40 days under the conditions of te...

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Abstract

The invention relates to plant tissue culture and discloses a tissue culture method for Vaccinium bracteatum Thunb, belonging to the field of plant cultivation. The method comprises the following four steps: explant sterilization; induction culture; propagation culture; and rooting culture. The method has the advantages of a high propagation rate, a fast growth rate and high hereditary stability, overcomes the problem of difficult breeding of Vaccinium bracteatum Thunb, can realize large-scale production and industrial large-scale supply of finished seedlings, protects excellent wild plant resources and brings about good economic values.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to plant tissue culture, in particular to a method for tissue culture of black rice tree. Background technique [0002] Black rice tree, also known as Nanzhu, belongs to the evergreen shrub of Rhododendronaceae. It is mostly distributed in the southern part of my country. It is named after people steam black rice with its leaves every year on the third day of March and the eighth day of April in the lunar calendar. The shape of the black rice tree is ancient and strong, the root is taupe and reddish, the posture is graceful, simple and elegant, and it is a rare and excellent material for making bonsai; the black rice made from the leaves of the black rice tree has the functions of nourishing the spleen and kidney, relieving cough, and calming the nerves. , eyesight, black hair effect. Because people eat the leaves of the black rice tree every year, and the land reclamation destroys ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李峰邓艳高秋荣田力许超
Owner 江苏九久环境科技有限公司
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