Method for breeding and regenerating plants from dormant buds of rice
A technology for regenerating plants and dormant buds, applied in the fields of botanical equipment and methods, rice cultivation, horticultural methods, etc., can solve the problems of weak transplanting, albino tissue culture seedlings, cumbersome seed production process, etc. Significant difference, easy to promote and apply
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Embodiment 1
[0030] FS (female fertility), fs (female sterility), CMS (cytoplasmic male sterility), Liyu B (conventional material), Dianza 37 (hybrid F 1 ) as the test material, the rice stalks with node dormant buds were spread and covered with soil, and the rice piles were directly transplanted as a control. Heavy investigations such as investigating the method of the present invention to some rice special materials, conventional materials, hybrid rice and other advantages such as emergence efficiency.
[0031] The specific method is as follows:
[0032] (1) Within 7 days after harvesting of FS, fs, CMS, Liyu B, and Dianza 37 off-season rice crops, the aboveground parts of FS, fs, CMS, Liyu B, and Dianza 37 off-season rice crops were intercepted with inverted 1 ~The rice stalks with dormant buds at 3 nodes, washed; The rice stalks with dormant buds at the inverted 1 and inverted 2 nodes, or the rice stalks with the dormant buds at the inverted 1, inverted 2, and inverted 3 nodes, consi...
Embodiment 2
[0038] FS, fs, CMS, Liyu B, and Dianza 37 were selected as test materials, and different gradients of KIN (kinin), 6-BA (6-benzyladenine), NAA (naphthaleneacetic acid) + KIN, 2, 4-D (2,4-dichlorophenoxyacetic acid) + KIN, NAA + 6-BA, 2,4-D + 6-BA solution concentration treatment, investigate the emergence rate and other items, compare different hormones, different hormones Combinations and emergence rates at different concentrations, etc., after several rounds of screening, the appropriate hormones, hormone combinations and concentration ratios were obtained.
[0039] The specific method is as follows:
[0040] (1) Within 7 days after harvesting of 37 heads of crop rice in FS, fs, CMS, Liyu B, and Dianza, intercept 37 off-ground parts of FS, fs, CMS, Liyu B, and Dianza with inverted 1 ~ Pour out the rice stalks with dormant buds at 3 nodes and wash them;
[0041] (2) The rice stalks of FS, fs, CMS, Liyu B, and Dianza 37 described in the step (1) were mixed in equal amounts, ...
Embodiment 3
[0082] Heline 42B (conventional rice maintainer), Liyu B (conventional rice maintainer), Dianza 38 (hybrid F 1 ) Rice stalks with dormant buds at the first to third nodes above the ground of the first crop rice were used as test materials, soaked in hormone solution 0.25mg / L 6-BA + 30mg / L NAA for 48 hours, and mixed with Heli 42B, Li The seeds of Yu B and Dianza 38 germinated and became seedlings as controls. The 3-4 leaf stage was transplanted to the experimental field, and the field cultivation and management were carried out according to the conventional field cultivation and management method of rice. Investigate and compare the emergence rate, dry matter weight at full tillering stage, leaf area index and chlorophyll content, panicle length at maturity, grain number, etc., to investigate the superiority of hormone-induced regeneration.
[0083] The specific method is as follows:
[0084] 1) Within 7 days after harvesting of Hexi 42B, Liyu B, and Dianza 37 off-season ri...
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