Method for breeding and regenerating plants from dormant buds of rice

A technology for regenerating plants and dormant buds, applied in the fields of botanical equipment and methods, rice cultivation, horticultural methods, etc., can solve the problems of weak transplanting, albino tissue culture seedlings, cumbersome seed production process, etc. Significant difference, easy to promote and apply

Active Publication Date: 2014-10-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the fact that the existing rice regenerated plants are only regenerated by directly using rice piles in the field, the output is unstable, the yield and production benefit are generally low, and the production cost of hybrid rice is high and the seed production process is cumbersome and special. The propagation of the material is difficult, the rice tissue culture seedlings are prone to death, albinism, and the tissue culture seedlings are relatively weak when transplanted to the field. The survival rate cannot be guaranteed, and there are defects and deficiencies in the high cost of large-scale production. The purpose of the present invention is to provide a A rice breeding method capable of obtaining regenerated rice plants efficiently, quickly and at low cost

Method used

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  • Method for breeding and regenerating plants from dormant buds of rice
  • Method for breeding and regenerating plants from dormant buds of rice
  • Method for breeding and regenerating plants from dormant buds of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] FS (female fertility), fs (female sterility), CMS (cytoplasmic male sterility), Liyu B (conventional material), Dianza 37 (hybrid F 1 ) as the test material, the rice stalks with node dormant buds were spread and covered with soil, and the rice piles were directly transplanted as a control. Heavy investigations such as investigating the method of the present invention to some rice special materials, conventional materials, hybrid rice and other advantages such as emergence efficiency.

[0031] The specific method is as follows:

[0032] (1) Within 7 days after harvesting of FS, fs, CMS, Liyu B, and Dianza 37 off-season rice crops, the aboveground parts of FS, fs, CMS, Liyu B, and Dianza 37 off-season rice crops were intercepted with inverted 1 ~The rice stalks with dormant buds at 3 nodes, washed; The rice stalks with dormant buds at the inverted 1 and inverted 2 nodes, or the rice stalks with the dormant buds at the inverted 1, inverted 2, and inverted 3 nodes, consi...

Embodiment 2

[0038] FS, fs, CMS, Liyu B, and Dianza 37 were selected as test materials, and different gradients of KIN (kinin), 6-BA (6-benzyladenine), NAA (naphthaleneacetic acid) + KIN, 2, 4-D (2,4-dichlorophenoxyacetic acid) + KIN, NAA + 6-BA, 2,4-D + 6-BA solution concentration treatment, investigate the emergence rate and other items, compare different hormones, different hormones Combinations and emergence rates at different concentrations, etc., after several rounds of screening, the appropriate hormones, hormone combinations and concentration ratios were obtained.

[0039] The specific method is as follows:

[0040] (1) Within 7 days after harvesting of 37 heads of crop rice in FS, fs, CMS, Liyu B, and Dianza, intercept 37 off-ground parts of FS, fs, CMS, Liyu B, and Dianza with inverted 1 ~ Pour out the rice stalks with dormant buds at 3 nodes and wash them;

[0041] (2) The rice stalks of FS, fs, CMS, Liyu B, and Dianza 37 described in the step (1) were mixed in equal amounts, ...

Embodiment 3

[0082] Heline 42B (conventional rice maintainer), Liyu B (conventional rice maintainer), Dianza 38 (hybrid F 1 ) Rice stalks with dormant buds at the first to third nodes above the ground of the first crop rice were used as test materials, soaked in hormone solution 0.25mg / L 6-BA + 30mg / L NAA for 48 hours, and mixed with Heli 42B, Li The seeds of Yu B and Dianza 38 germinated and became seedlings as controls. The 3-4 leaf stage was transplanted to the experimental field, and the field cultivation and management were carried out according to the conventional field cultivation and management method of rice. Investigate and compare the emergence rate, dry matter weight at full tillering stage, leaf area index and chlorophyll content, panicle length at maturity, grain number, etc., to investigate the superiority of hormone-induced regeneration.

[0083] The specific method is as follows:

[0084] 1) Within 7 days after harvesting of Hexi 42B, Liyu B, and Dianza 37 off-season ri...

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Abstract

The invention discloses a method for breeding regeneration plants by rice dormant buds, and belongs to the technical field of crop seedling. According to the method, rice straws of rice with reciprocal 1 to reciprocal 3 sections of dormant buds are taken to be scattered and planted onto a seedling culture bed, soil is covered, or the rice straws are soaked by plant hormone, then, the rice straws are scattered and planted onto the seedling culture bed, the soil is covered, the seedling culture and the management are carried out according to the conventional seedling culture method, and the seedlings are transplanted to the field after the regeneration seedlings reach 3-leaf to 4-leaf period. The method has the advantages that the effective storage and breeding can be realized for obtaining a large number of special rice materials including female sterile, male sterile and the like, the difficult problem of breeding difficulty of special materials is solved, hybrid F1 can be directly utilized for obtaining a large number of F1 regeneration plants, the rice hybrid seed production cost can be effectively reduced, the breeding period is shortened, and the hybrid advantages can be perfectly maintained. According to the method, the rice overground parts with rice straws with reciprocal 1 to reciprocal 3 sections of dormant buds are adopted, both the regeneration efficiency of the rice straws treated by hormone and the regeneration efficiency of the rice straws which are not treated by hormone are obviously superior to that of rice pile direct regeneration, the method is simple, the implementation is easy, and the popularization and the application are convenient.

Description

technical field [0001] The invention relates to a method for obtaining regenerated plants by using dormant buds of rice, which belongs to the field of agricultural biotechnology, more specifically, belongs to the field of crop breeding technology. Background technique [0002] Rice ( Oryza sativa. L) is the third largest food crop in the world next to corn and wheat, and rice is the staple food for more than half of the population in the world. my country is a major rice production and consumption country in the world. Rice cultivation area accounts for 1 / 3 of the grain crop planting area, and its output accounts for nearly 1 / 2 of the total grain output. The per capita annual consumption of rice is more than 150kg. Therefore, rice production is related to the overall situation of national food security. With the continuous improvement of modern rice production technology, world-renowned results have been achieved in the improvement of rice yield and quality, which has great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G16/00A01G7/06A01G22/22
Inventor 李东宣陈丽娟谭学林吕永刚孙明姬朱有勇李成云
Owner YUNNAN AGRICULTURAL UNIVERSITY
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