Zinc-rich saccharomyces cerevisiae and production method thereof

A zinc-rich yeast and production method technology, applied in the field of microorganisms, can solve the problems of low yield, low organic zinc content, low zinc utilization rate, etc., and achieve the effects of stable zinc resistance, high biological zinc content, and fast growth rate

Active Publication Date: 2009-11-11
ANGEL NUTRITECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been many studies on zinc-rich yeast at home and abroad, but the content is not very high. The main problems are: (1) The strains of zinc-rich yeast are not screened enough, and the strains screened out are not strong in enriching zinc. , the zinc content in the yeast is not high (2) the organic zinc content is low, and some of them are simply adsorbed or mixed with yeast and zinc, and the inorganic zinc is not converted into organic zinc; (3) some methods are not suitable for large-scale production, and the repeatability is very high Poor, some can be produced, but the yield is very low and the production cost is high; (4) The utilization rate of zinc is low

Method used

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the present invention is to the screening of saccharomyces cerevisiae strain

[0033] 1) Screening of yeast strains resistant to high zinc: Inoculate Saccharomyces cerevisiae into zinc-containing medium with different concentrations, and screen strains that can grow in high zinc medium. The strains resistant to zinc were selected as domesticated strains.

[0034] 2) Inoculate zinc-resistant strains on a medium with high concentration of zinc for cultivation, and gradually increase the concentration of zinc in the medium to maintain a higher concentration of zinc. Select high-zinc-resistant and stable strains as mutagenic strains.

[0035] 3) inoculating the selected strains into culture media with different concentrations of lithium chloride for mutagenesis.

[0036] 4) Separation and screening of the mutagenized strains, using shake flasks and small fermenters for fermentation to select high-zinc-resistant yeast strains with high zinc content, fast grow...

Embodiment 2

[0041] Embodiment 2: the production of zinc-enriched yeast product

[0042] The production process of producing zinc-rich yeast with high zinc content: slant strain cultivation→first-level liquid seed cultivation→secondary liquid seed cultivation→fermentation tank seed cultivation→fermentation tank cultivation→separation→drying→packaging. The specific process is as follows:

[0043] 1) Slant strain culture: inoculate the zinc-rich yeast strain resistant to high zinc onto the wort solid slope with a sugar content of 10%, a zinc (amino acid chelated zinc) concentration of 150 μg / ml, and a pH value of 5.5. Incubate at 30°C for 24 hours.

[0044] 2) First-level liquid seed culture: Inoculate 3 rings of yeast strains on the inclined plane to 250ml of sugar (wort and molasses half and half) amount of 10%, zinc (amino acid chelated zinc) concentration of 150 μg / ml, Incubate at 30°C for 24 hours in special wort culture bottles with a pH value of 5.5.

[0045] 3) Secondary liquid se...

Embodiment 3

[0051] Embodiment 3: the production of zinc-enriched yeast product

[0052] The production process of producing zinc-rich yeast with high zinc content: slant strain cultivation→first-level liquid seed cultivation→secondary liquid seed cultivation→fermentation tank seed cultivation→fermentation tank cultivation→separation→drying→packaging. The specific process is as follows:

[0053] 1) Slant strain culture: Inoculate the zinc-rich yeast strain resistant to high zinc onto the wort solid slope with a sugar content of 10%, a zinc (amino acid chelated zinc) concentration of 500 μg / ml, and a pH value of 5.5. Incubate at 30°C for 24 hours.

[0054] 2) First-level liquid seed culture: Inoculate 4 rings of yeast strains on the inclined plane to 500 ml of sugar-containing (starch hydrolyzed sugar) content of 10%, zinc (amino acid chelated zinc) concentration of 500 μg / ml, and a pH of 500 ml. 5.5 in the wort special culture bottle, cultured at 30 ℃ for 24 hours.

[0055] 3) secondary...

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Abstract

The invention provides a zinc-rich saccharomyces cerevisiae Z1.4CCTCC M 205126. The zinc-rich saccharomyces cerevisiae has stable zinc resistance, fast growing speed and high utility ratio of zinc and contains zinc as high as 80,000mg / kg, wherein the content of biological zinc can reach 90 percent.

Description

technical field [0001] The invention relates to a microorganism, in particular to a zinc-rich yeast, and also relates to a breeding method for the strain and a method for industrial production using the strain. Background technique [0002] Zinc is an essential trace element for the human body and animals. In the living body, zinc is not only a component of many enzymes, but also can affect the institutional configuration of some non-enzyme organic molecular ligands, which plays a very important role in maintaining the normal physiological functions of the human body. important role. Zinc deficiency in the human body can cause many diseases, such as dwarfism, diabetes, high blood pressure, hypoplasia of genitals and secondary sexual characteristics, male infertility and other diseases. The normal physiological metabolism of the human body will lose a part of zinc, and the zinc supplemented in the food is not enough to meet the normal metabolic zinc of the human body, thus l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12R1/865
Inventor 俞学锋李知洪余明华姚娟张彦朱娅敏
Owner ANGEL NUTRITECH CO LTD
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